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    Wyświetlanie 1-4 z 4
    Tytuł:
    Effects of Bee Venom on Activity and Expression of 15-Lipoxygenase-1 in Human HT29 Colon Cancer
    Autorzy:
    soukhtanloo, Mohammad
    Zare, Maryam
    Khayatzadeh, Jina
    Sadegh Nia, Hamidreza
    Mojarad, Majid
    Baghbani, Fatemeh
    Sargolzaei, Javad
    Sadeghian, Hamid
    Sisakhti, Mohsen
    Powiązania:
    https://bibliotekanauki.pl/articles/895438.pdf
    Data publikacji:
    2019-02-28
    Wydawca:
    Polskie Towarzystwo Farmaceutyczne
    Tematy:
    apoptosis
    HPLC
    15-lipoxygenase-1
    Bee Venom
    HT-29
    Colerectal Cancer
    Opis:
    Colorectal cancer has a high incidence rate with significant mortality and morbidity. The 15-lipoxygenase-1 (15-LOX-1) is a crucial enzyme as well as a tumor suppressor, especially in colon cancer. Bee venom, by the induction of apoptosis, is a promising new anti-cancer agent. Thus, the effects of bee venom on the expression of 15-LOX-1 m-RNA and its activities in the HT-29 cell line have been investigated in the current study. Human HT-29 colon cancer cells were treated with increasing concentrations of bee venom (1.87-30 µg/mL), and cisplatin (5 µg/mL) as the positive control for 24- and 48-hours. Then, cell viability and apoptosis were measured using the MMT assay and flow cytometry, respectively. Enzyme activity and expression was assayed using the kinetic method and real-time PCR, respectively. The results showed that the main content of bee venom was melittin with a retention time of 60 minutes. The IC50 values of bee venom at 24- and 48-hours were 6.01 µg/mL and 4.44 µg/mL, respectively. Expression of 15-LOX-1 in cancer cells treated with bee venom increased (p < 0.0005), as well as the activity of the enzyme in the presence of bee venom (p < 0.01). The current study revealed the apoptotic and cytotoxic effects of bee venom against the human colon cancer HT-29 cell line that was not seen in fibroblast cells. Findings suggest that bee venom may have therapeutic effects against the HT-29 colon cancer cell through the induction of the15-LOX-1 pathway. However, further studies are needed in this regard.
    Źródło:
    Acta Poloniae Pharmaceutica - Drug Research; 2019, 76, 1; 75-82
    0001-6837
    2353-5288
    Pojawia się w:
    Acta Poloniae Pharmaceutica - Drug Research
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Quantitative analysis of the level of p53 and p21WAF1 mRNA in human colon cancer HT-29 cells treated with inositol hexaphosphate
    Autorzy:
    Węglarz, Ludmiła
    Molin, Izabela
    Orchel, Arkadiusz
    Parfiniewicz, Beata
    Dzierżewicz, Zofia
    Powiązania:
    https://bibliotekanauki.pl/articles/1041248.pdf
    Data publikacji:
    2006
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    p21WAF1
    inositol hexaphosphate
    RT-PCR
    HT-29 cells
    p53
    Opis:
    The aim of this study was to analyze the molecular mechanism of inositol hexaphosphate (InsP6) action through which it may inhibit proliferation of colon cancer cells and cell cycle progression. A kinetic study of p53 and p21WAF1 mRNA increase was performed on human colon cancer HT-29 cells after treatment with 1, 5 and 10 mM InsP6 for 6, 12, 24 and 48 h. Real-time-QPCR based on TaqMan methodology was applied to analyze quantitatively the transcript levels of these genes. The transcription of β-actin and GAPDH genes was assessed in parallel to select the control gene with least variability. The 2-ΔΔCt method was used to analyze the relative changes in gene transcription. InsP6 stimulated p53 and p21WAF1 expression at the mRNA level, with the highest increase in p21WAF1 mRNA occurring at 24 h, i.e., following the highest increase in p53 mRNA observed at 12 h. Based on these studies it may be concluded that the ability of InsP6 to arrest the cell cycle may be mediated by the transcriptional up-regulation of the p53-responsive p21WAF1 gene.
    Źródło:
    Acta Biochimica Polonica; 2006, 53, 2; 349-356
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    An integrated gas removing system for microfluidic application. Design and evaluation
    Autorzy:
    Hofman, I. M.
    Ziółkowska, K.
    Dybko, A.
    Brzózka, Z.
    Powiązania:
    https://bibliotekanauki.pl/articles/115931.pdf
    Data publikacji:
    2013
    Wydawca:
    Fundacja na Rzecz Młodych Naukowców
    Tematy:
    gas removing system
    debubbler
    debubbling system
    lab-on-a-chip
    microfluidics
    long-term cell culture
    cell spheroids
    HT-29 cells
    Opis:
    Microfluidic systems are used in a wide range of applications, including medical diagnostics, cell engineering and bioanalytics. In this work we focused on “Lab-on-a-chip” microsystems for cell cultivation. A troublesome problem of gas bubbles entering microdevices causing signal interferences and cells damage was emphasized. A novel, integrated debubbler in the form of cylindrical traps covered with thin PDMS membrane was designed and manufactured. Demonstrated debubbler was successfully applied in a long-lasting culture of HT-29 cell aggregates.
    Źródło:
    Challenges of Modern Technology; 2013, 4, 3; 10-15
    2082-2863
    2353-4419
    Pojawia się w:
    Challenges of Modern Technology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Ion and Electron Beam Induced Luminescence οf Rare Earth Doped YAG Crystals
    Autorzy:
    Gawlik, G.
    Sarnecki, J.
    Jóźwik, I.
    Jagielski, J.
    Pawłowska, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/1504113.pdf
    Data publikacji:
    2011-07
    Wydawca:
    Polska Akademia Nauk. Instytut Fizyki PAN
    Tematy:
    78.60.Hk
    52.59.Bi
    79.20.Rf
    61.80.Lj
    41.75.Ak
    41.75.Cn
    61.72.S-
    61.72.-y
    29.40.-n
    77.84.Bw
    79.60.Ht
    87.53.Bn
    78.55.-m
    78.60.-b
    77.55.Px
    77.55.-g
    25.40.Lw
    Opis:
    The aim of this work was the evaluation of ion-beam induced luminescence for the characterization of luminescent oxide materials containing rare earth elements. The yttrium aluminium garnet epilayers doped with Nd, Pr, Ho, and Tm atoms were used. The ion-beam induced luminescence spectra were excited using 100 keV $H_2^{+}$ ion beam and were recorded in the wavelengths ranging from 300 nm up to 1000 nm. The separate parts of the surface of the same samples were used for ion-beam induced luminescence and cathodoluminescence experiments. Cathodoluminescence spectra have been recorded in the range from 370 nm up to 850 nm at 20 keV e-beam in scanning electron microscope equipped with a grating spectrometer coupled with a photomultiplier. The observed narrow ion-beam induced luminescence lines can be ascribed to the well known radiative transitions in the rare-earth ions in the YAG crystals. The cathodoluminescence spectra reveal essentially the same emission lines as ion-beam induced luminescence. The decrease of the ion-beam induced luminescence lines intensity has been observed under the increasing ion fluences. The ion-beam induced luminescence may be used for characterization of transparent luminescent materials as an alternative method for cathodoluminescence and can be especially useful for observation of ion-beam damage formation in crystals.
    Źródło:
    Acta Physica Polonica A; 2011, 120, 1; 181-183
    0587-4246
    1898-794X
    Pojawia się w:
    Acta Physica Polonica A
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-4 z 4

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