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Wyszukujesz frazę "Sikorski, Aleksander" wg kryterium: Autor

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Wyświetlanie 1-10 z 15
Tytuł:
Application of genetic semihomology algorithm to theoretical studies on various protein families.
Autorzy:
Leluk, Jacek
Hanus-Lorenz, Beata
Sikorski, Aleksander
Powiązania:
https://bibliotekanauki.pl/articles/1044159.pdf
Data publikacji:
2001
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
multiple alignment
cryptic mutations
genetic semihomology
sequence consensus
Opis:
Several protein families of different nature were studied for genetic relationship, correct alignment at non-homologous fragments, optimal sequence consensus construction, and confirmation of their actual relevance. A comparison of the genetic semihomology approach with statistical approaches indicates a high accuracy and cognition significance of the former. This is particularly pronounced in the study of related proteins that show a low degree of homology. The sequence multiple alignments were verified and corrected with respect to the questionable, non-homologous fragments. The verified alignments were the basis for consensus sequence formation. The frequency of six-codon amino acids occurrence versus position variability was studied and their possible role in amino acid mutational exchange at variable positions is discussed.
Źródło:
Acta Biochimica Polonica; 2001, 48, 1; 21-33
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Phosphatidic acid - a simple phospholipid with multiple faces
Autorzy:
Zegarlińska, Jolanta
Piaścik, Magda
Sikorski, Aleksander
Czogalla, Aleksander
Powiązania:
https://bibliotekanauki.pl/articles/1038384.pdf
Data publikacji:
2018
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
phosphatidic acid
protein-lipid interaction
signaling
membrane curvature
membrane model systems
Opis:
Phosphatidic acid (PA) is the simplest glycerophospholipid naturally occurring in living organisms, and even though its content among other cellular lipids is minor, it is drawing more and more attention due to its multiple biological functions. PA is a precursor for other phospholipids, acts as a lipid second messenger and, due to its structural properties, is also a modulator of membrane shape. Although much is known about interaction of PA with its effectors, the molecular mechanisms remain unresolved to a large degree. Throughout many of the well-characterized PA cellular sensors, no conserved binding domain can be recognized. Moreover, not much is known about the cellular dynamics of PA and how it is distributed among subcellular compartments. Remarkably, PA can play distinct roles within each of these compartments. For example, in the nucleus it behaves as a mitogen, influencing gene expression regulation, and in the Golgi membrane it plays a role in membrane trafficking. Here, we discuss how a biophysical experimental approach enabled PA behavior to be described in the context of a lipid bilayer and to what extent various physicochemical conditions may modulate the functional properties of this lipid. Understanding these aspects would help to unravel specific mechanisms of PA-driven membrane transformations and protein recruitment and thus would lead to a clearer picture of the biological role of PA.
Źródło:
Acta Biochimica Polonica; 2018, 65, 2; 163-171
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Attaching a spin to a protein - site-directed spin labeling in structural biology
Autorzy:
Czogalla, Aleksander
Pieciul, Aldona
Jezierski, Adam
Sikorski, Aleksander
Powiązania:
https://bibliotekanauki.pl/articles/1041067.pdf
Data publikacji:
2007
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
protein structure
site-directed spin labeling
electron paramagnetic resonance (EPR) spectroscopy
Opis:
Site-directed spin labeling and electron paramagnetic resonance spectroscopy have recently experienced an outburst of multiple applications in protein science. Numerous interesting strategies have been introduced for determining the structure of proteins and its conformational changes at the level of the backbone fold. Moreover, considerable technical development in the field makes the technique an attractive approach for the study of structure and dynamics of membrane proteins and large biological complexes at physiological conditions. This review focuses on a brief description of site-directed spin labeling-derived techniques in the context of their recent applications.
Źródło:
Acta Biochimica Polonica; 2007, 54, 2; 235-244
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Optimization of flotation assay conditions for syndapin binding to phosphatidic acid containing liposomes
Autorzy:
Piaścik, Magda
Zegarlińska, Jolanta
Sikorski, Aleksander F.
Czogalla, Aleksander
Powiązania:
https://bibliotekanauki.pl/articles/764838.pdf
Data publikacji:
2017
Wydawca:
Uniwersytet Łódzki. Wydawnictwo Uniwersytetu Łódzkiego
Tematy:
protein-lipid interactions
LUVs
density gradient
ultracentrifugation
Opis:
Flotacja jest jedną z najefektywniejszych metod wstępnej identyfikacji oddziaływań białko-błony lipidowe. W większości przypadków wykorzystuje się w niej małe jednowarstwowe pęcherzyki lipidowe, które służą jako modele błonowe i nie wymagają dodatkowych nośników, takich jak membrany czy nanocząstki polimerowe, które są często używane w innych metodach mających na celu identyfikację oddziaływań białkolipid. W poniższej pracy prezentujemy wyniki uzyskane podczas badań oddziaływań kwasu fosfatydowego i syndapiny. Omawiamy także niektóre techniczne aspekty metody, kładąc nacisk na to jak małe zmiany w warunkach metody mogą wpłynąć na otrzymywane wyniki.
Flotation is one of the best method for preliminary identification of protein-lipid interactions. In most widely used approach it utilizes large unilamellar vesicles, that are excellent models of freestanding membranes and do not require any additional components, like solid supports or beads that are needed in other methods commonly used for protein-lipid binding studies. Here we present results obtained during our studies on phosphatidic acid - syndapin interactions and discuss some technical aspects of this method underlying how relatively small changes in the conditions can influence the results.
Źródło:
Acta Universitatis Lodziensis. Folia Biologica et Oecologica; 2017, 13; 9-17
1730-2366
2083-8484
Pojawia się w:
Acta Universitatis Lodziensis. Folia Biologica et Oecologica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Interaction of membrane skeletal proteins with membrane lipid domain.
Autorzy:
Sikorski, Aleksander
Hanus-Lorenz, Beata
Jezierski, Adam
Dluzewski, Anton
Powiązania:
https://bibliotekanauki.pl/articles/1044290.pdf
Data publikacji:
2000
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
non-erythroid cells
membrane skeleton
membrane lipid domains
cytoskeleton
red blood cells
Opis:
The object of this paper is to review briefly the studies on the interaction of red blood cell membrane skeletal proteins and their non-erythroid analogues with lipids in model systems as well as in natural membranes. An important question to be addressed is the physiological significance and possible regulatory molecular mechanisms in which these interactions are engaged.
Źródło:
Acta Biochimica Polonica; 2000, 47, 3; 565-578
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Expression, purification and functional characterization of recombinant human acyl-CoA-binding protein (ACBP) from erythroid cells
Autorzy:
Augoff, Katarzyna
Kolondra, Adam
Chorzalska, Anna
Lach, Agnieszka
Grabowski, Krzysztof
Sikorski, Aleksander
Powiązania:
https://bibliotekanauki.pl/articles/1040319.pdf
Data publikacji:
2010
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
acyl-CoA-binding protein
PC
DBI
ACBP
phosphatidylcholine
Opis:
Fatty acyl-CoA esters are extremely important in cellular homeostasis. They are intermediates in both lipid metabolism and post-translational protein modifications. Among these modification events, protein palmitoylation seems to be unique by its reversibility which allows dynamic regulation of the protein hydrophobicity. The recent discovery of an enzyme family that catalyze protein palmitoylation has increased the understanding of the enzymology of the covalent attachment of fatty acids to proteins. Despite that, the molecular mechanism of supplying acyl-CoA esters to this reaction is yet to be established. Acyl-coenzyme A-binding proteins are known to bind long-chain acyl-CoA esters with very high affinity. Therefore, they play a significant role in intracellular acyl-CoA transport and pool formation. The purpose of this work is to explore the potential of one of the acyl-CoA-binding proteins to participate in the protein palmitoylation. In this study, a recombinant form of ACBP derived from human erythroid cells was expressed in E. coli, purified, and functionally characterized. We demonstrate that recombinant hACBP effectively binds palmitoyl-CoA in vitro, undergoing a shift from a monomeric to a dimeric state, and that this ligand-binding ability is involved in erythrocytic membrane phosphatidylcholine (PC) remodeling but not in protein acylation.
Źródło:
Acta Biochimica Polonica; 2010, 57, 4; 533-540
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-10 z 15

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