- Tytuł:
- Influence of change in the proportion of H1 histone variants on microsporogenesis and development of male gametophyte in transgenic plants of tobacco [Nicotiana tabacum L.]
- Autorzy:
-
Slusarczyk, J
Wierzbicki, A.
Przewloka, M.
Tykarska, T.
Jerzmanowski, A.
Kuras, M. - Powiązania:
- https://bibliotekanauki.pl/articles/57064.pdf
- Data publikacji:
- 2003
- Wydawca:
- Polskie Towarzystwo Botaniczne
- Tematy:
-
sterile pollen grain
male
development
transgenic plant
gametophyte
tobacco
microsporogenesis disorder
histone H1
Nicotiana tabacum - Opis:
- As continuation of investigations in to the mechanism of the role of the H1 histone, which is a crucial protein component chromosomes of all eukaryotes, transgenic tobacco plants with different levels of the H1 histone variants were examined. Tobacco has six sequential variants of the H1 histone: two major ones (H1A and H1B), constituting ca. 90% of all H1, and four minor ones (H1C, H1D, H1E and H1F), occurring in very small quantities. The following groups of plants were examined: K - control group with a full set of histone variants; -AB -with the A and B variants removed; -ABCD - with the A, B, C and D variants removed; and -CD - with the C and D variants removed. The analysis of microsporogenesis in those plants, based on preparations squeezed in acetoorcein, revealed the asynchronous course of meiosis in -AB and -ABCD plants, occurrence of chromosomal aberration, and, consequently, the formation of sterile pollen grains (accordingly: 84,4% and 81,4%). In -CD plants, the percentage of aberration and sterile pollen grains was similar to the control material. Electron microscope observations of microsporogenesis showed ultrastructural changes. In -AB and -ABCD plants, a major portion of the pollen grains were degraded. The smallest number of degraded pollen grains, in comparison with the control, was found in the -CD group.
- Źródło:
-
Acta Societatis Botanicorum Poloniae; 2003, 72, 1
0001-6977
2083-9480 - Pojawia się w:
- Acta Societatis Botanicorum Poloniae
- Dostawca treści:
- Biblioteka Nauki
Artykuł