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    Wyświetlanie 1-4 z 4
    Tytuł:
    Protoplast isolation and plant regeneration in two cultivated coriander varieties, Co-1 and RS
    Autorzy:
    Ali, M.
    Mujib, A.
    Zafar, N.
    Tonk, D.
    Powiązania:
    https://bibliotekanauki.pl/articles/80271.pdf
    Data publikacji:
    2018
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    plant regeneration
    Coriandrum sativum
    coriander
    cultural variety
    plant growth regulator
    2,4-dichlorophenoxyacetic acid
    6-benzyladenine
    alpha-naphthalene acetic acid
    gibberellic acid
    callus induction
    somatic embryogenesis
    Źródło:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2018, 99, 4
    0860-7796
    Pojawia się w:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Quantification of taxol by high-performance thin layer chromatography in Taxus wallichiana callus cultivated in vitro
    Autorzy:
    Mujib, A.
    Tonk, D.
    Gulzar, B.
    Maqsood, M.
    Ali, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/2096321.pdf
    Data publikacji:
    2020
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    antioxidant enzymes
    callus induction
    stress
    taxol
    Taxus wallichiana
    2
    4-dichlorophenoxyacetic
    acid
    Opis:
    In the present study, the induction of callus, callus biomass growth and the yield of taxol were investigated in Taxus wallichiana. This is the first report of a quantification study of taxol in in vitro grown tissues of Taxus obtained from Jammu and Kashmir provinces, India. For callus induction, three different explant types (leaf, cone and stem) were cultured in media supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D), naphthaleneacetic acid (NAA) and 6-benzylaminopurine (BAP). The stem was most responsive to callusing, and the maximum callus induction frequency of 71.5% was noted on medium amended with 2.0 mg/l 2,4-D. The medium amended with 2,4-D and ascorbic acid (AA) showed better callus growth and the maximum biomass (0.082 g, fresh weight). The yield of taxol was quantified in the callus grown on a medium supplemented with different plant growth regulators (PGRs) by using high-performance thin layer chromatography (HPTLC). The taxol yield was maximum (1.053 μg/g dry weight) in 2,4-D-stimulated callus, followed by callus treated with NAA in which 0.896 μg/g dry weight taxol was detected. The maximum taxol yield (56.6%) was obtained in the callus grown on the medium amended with 2,4-D, and a 33.3% increased yield was noted on the NAA-supplemented medium. As a stress marker, the activities of the antioxidant enzymes superoxide dismutase (SOD) and ascorbate peroxidase (APX) and the level of proline were measured in an auxin (2,4-D and NAA)-supplemented medium. The callus grown on the 2,4-D-supplemented medium had high levels of SOD (3.91 min-1 * mg-1 protein), APX (1.61 min -1 * mg-1 protein) and proline (6.57 mg/g), thus suggesting a higher stress level; the callus grown on the NAA-supplemented medium had slightly lower levels of SOD and APX enzyme activities and proline content (3.01 min-1 * mg -1 protein, 1.04 min-1 * mg -1 protein and 5.90 mg/g, respectively). BAP had little influence on stress parameters. The present study thus indicates a good taxol yield in the callus cultured in 2,4-D, which functioned as a signalling element and a stressor. The taxol yield in response to PGRs was analysed in in vitro cultivated tissues.
    Źródło:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2020, 101, 4; 337-347
    0860-7796
    Pojawia się w:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    In vitro propagation of Althaea officinalis: the role of plant growth regulators in morphogenesis
    Autorzy:
    Mujib, A.
    Pipal, T.
    Ali, M.
    Tonk, D.
    Zafar, N.
    Gulzar, B.
    Powiązania:
    https://bibliotekanauki.pl/articles/79918.pdf
    Data publikacji:
    2017
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    Althaea officinalis
    marshmallow
    in vitro propagation
    clonal multiplication
    2,4-dichlorophenoxyacetic acid
    plant growth regulator
    morphogenesis
    rooting
    Źródło:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2017, 98, 3
    0860-7796
    Pojawia się w:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-4 z 4

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