- Tytuł:
- pH Profile of cytochrome c-catalyzed tyrosine nitration
- Autorzy:
-
Kambayashi, Yasuhiro
Hitomi, Yoshiaki
Kodama, Norio
Kubo, Masayuki
Okuda, Junna
Takemoto, Kei
Shibamori, Masafumi
Takigawa, Tomoko
Ogino, Keiki - Powiązania:
- https://bibliotekanauki.pl/articles/1041220.pdf
- Data publikacji:
- 2006
- Wydawca:
- Polskie Towarzystwo Biochemiczne
- Tematy:
-
pseudo-peroxidase
pH
cytochrome c
nitrite
nitrotyrosine - Opis:
- In the present study, we investigated how cytochrome c catalyzed the nitration of tyrosine at various pHs. The cytochrome c-catalyzed nitration of tyrosine occurred in proportion to the concentration of hydrogen peroxide, nitrite or cytochrome c. Thecytochromec-catalyzed nitration of tyrosine was inhibited by catalase, sodium azide, cystein, and uric acid. These results show that the cytochrome c-catalyzednitrotyrosine formation was due to peroxidase activity. The rate constant between cytochrome c and hydrogen peroxide within the pH range of 3 - 8 was the largest at pH 6 (37°C). The amount of nitrotyrosine formed was the greatest at pH 5. At pH 3, onlycytochromec-independent nitration of tyrosine occurred in the presence of nitrite. At this pH, the UV as well as visible spectrum of cytochrome c was changed by nitrite, even in the presence of hydrogen peroxide, probably via the formation of a heme iron - nitric oxide complex. Due to this change, the peroxidase activity of cytochrome c was lost.
- Źródło:
-
Acta Biochimica Polonica; 2006, 53, 3; 577-584
0001-527X - Pojawia się w:
- Acta Biochimica Polonica
- Dostawca treści:
- Biblioteka Nauki
Artykuł