- Tytuł:
- Protein thermal stabilization in aqueous solutions of osmolytes
- Autorzy:
-
Bruździak, Piotr
Panuszko, Aneta
Jourdan, Muriel
Stangret, Janusz - Powiązania:
- https://bibliotekanauki.pl/articles/1038842.pdf
- Data publikacji:
- 2016
- Wydawca:
- Polskie Towarzystwo Biochemiczne
- Tematy:
-
water structure
protein stability
circular dichroism
FT-IR spectroscopy
DFT calculations - Opis:
- Proteins' thermal stabilization is a significant problem in various biomedical, biotechnological, and technological applications. We investigated thermal stability of hen egg white lysozyme in aqueous solutions of the following stabilizing osmolytes: Glycine (GLY), N-methylglycine (NMG), N,N-dimethylglycine (DMG), N,N,N-trimethylglycine (TMG), and trimethyl-N-oxide (TMAO). Results of CD-UV spectroscopic investigation were compared with FTIR hydration studies' results. Selected osmolytes increased lysozyme's thermal stability in the following order: Gly>NMG>TMAO≈DMG>TMG. Theoretical calculations (DFT) showed clearly that osmolytes' amino group protons and water molecules interacting with them played a distinctive role in protein thermal stabilization. The results brought us a step closer to the exact mechanism of protein stabilization by osmolytes.
- Źródło:
-
Acta Biochimica Polonica; 2016, 63, 1; 65-70
0001-527X - Pojawia się w:
- Acta Biochimica Polonica
- Dostawca treści:
- Biblioteka Nauki
Artykuł