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Wyświetlanie 16-30 z 34
Tytuł:
Isolation of wheat germ ribosomes free of high molecular weight inhibitors of the natural messenger translation
Autorzy:
Rychlik, Wojciech
Matie-Zabala, Minehaha
Zagórski, Włodzimierz
Powiązania:
https://bibliotekanauki.pl/articles/1046229.pdf
Data publikacji:
1979
Wydawca:
Polskie Towarzystwo Biochemiczne
Źródło:
Acta Biochimica Polonica; 1979, 26, 1-2; 115-123
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Cyrano de Bergerac
Autorzy:
Rostand, Edmond
Współwytwórcy:
Zagórski, Włodzimierz
Choromańska, Paulina
Konopnicka, Maria
Kotwica, Wojciech
Kopeć, Aleksandra
Data publikacji:
2019-09-12
Wydawca:
Fundacja Nowoczesna Polska
Tematy:
Komedia
Dramat
Modernizm
Opis:
Publikacja zrealizowana w ramach projektu Wolne Lektury (http://wolnelektury.pl). Dofinansowano ze środków Ministra Kultury i Dziedzictwa Narodowego.
Źródło:
Edmund Rostand, Cyrano de Bergerac, Nakładem Redakcyi "Gazety Polskiej", Warszawa 1898.
Dostawca treści:
Wolne Lektury
Książka
Tytuł:
Mapping of the influenza A hemagglutinin serotypes evolution by the ISSCOR method
Autorzy:
Radomski, Jan
Słonimski, Piotr
Zagórski-Ostoja, Włodzimierz
Borowicz, Piotr
Powiązania:
https://bibliotekanauki.pl/articles/1039244.pdf
Data publikacji:
2014
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
ISSCOR descriptors
phylogenetic analysis
influenza virus
hemagglutinin
phylogenetic maps
Opis:
Analyses and visualizations by the ISSCOR method of influenza virus hemagglutinin genes of different A-subtypes revealed some rather striking temporal relationships between groups of individual gene subsets. Based on these findings we consider application of the ISSCOR-PCA method for analyses of large sets of homologous genes to be a worthwhile addition to a toolbox of genomics - allowing for a rapid diagnostics of trends, and ultimately even aiding an early warning of newly emerging epidemiological threats.
Źródło:
Acta Biochimica Polonica; 2014, 61, 3; 441-451
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Co-inoculation with two non-infectious cDNA copies of potato spindle tuber viroid (PSTVd) leads to the appearance of novel fully infectious variants.
Autorzy:
Podstolski, Wojciech
Góra-Sochacka, Anna
Zagórski, Włodzimierz
Powiązania:
https://bibliotekanauki.pl/articles/1041466.pdf
Data publikacji:
2005
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
non-coding RNA
viroid
circular RNA
PSTVd
recombination
Opis:
Potato spindle tuber viroid (PSTVd) is one of the smallest (about 360 nt) infectious plant agents. It is composed of a single-stranded circular non-coding RNA molecule. In the course of previous passage experiments with two intermediate PSTVd variants I2 and I4, three non-infectious clones (I2-50, I4-37 and I4 VI-17) were found. When inoculated separately as cDNAs on tomato "Rutgers" test plants these variants did not induce any visible disease symptoms and did not produce progeny. The presence of such non-infectious variants raises several questions about their origin and biology and to answer them, mixed co-infections with cDNA copies of two non-infectious variants (I2-50, I4-37) were performed. PSTVd infection was observed in seven out of 30 inoculated plants. The progeny isolated from three separate plants contained novel variants, together with the parental I2 and I4 sequences. It is conceivable that the appearance of repaired PSTVd molecules, clearly capable of cell-to-cell movement leading to the systemic infection, results from recombination events. An analysis of the recombinant molecules and comparison with databases identified the specific sites responsible for the restricted infectivity of the I2-50 and I4-37 PSTVd variants. In parallel experiments in which (+) strand PSTVd infectious transcripts were used, no recombinants were observed, and the original I2-50 and I4-37 non-infectious sequences were not detected in the progeny.
Źródło:
Acta Biochimica Polonica; 2005, 52, 1; 87-98
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Nucleotide sequence of RNA of a Polish isolate of potato leafroll luteovirus
Autorzy:
Pałucha, Andrzej
Sadowy, Ewa
Kujawa, Alicja
Juszczuk, Marek
Zagórski, Włodzimierz
Hulanicka, Danuta
Powiązania:
https://bibliotekanauki.pl/articles/1045304.pdf
Data publikacji:
1994
Wydawca:
Polskie Towarzystwo Biochemiczne
Źródło:
Acta Biochimica Polonica; 1994, 41, 4; 405-414
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Community cohesion, well-being, and local development
Autorzy:
Okrasa, Włodzimierz
Rozkrut, Dominik
Domański, Czesław
Zagórski, Krzysztof
Data publikacji:
2021-12
Wydawca:
Główny Urząd Statystyczny
Opis:
This publication features a collection of papers discussing the factors which have an impact on the quality of life and the subjective sense of well-being of local communities. The articles also provide empirical explanations as to what extent the developmental levels of the regions studied affect quality life and well-being. The publication is a product of the Community Cohesion and Well-Being and Innovative Local Development international scientific conference, organised jointly by the Institute of Sociology of the Cardinal Stefan Wyszyński University in Warsaw and Statistics Poland (held on 4 December 2018). The innovative character of this scientific event is mutually beneficial for its organisers. The joint initiative of the university and the main producer of statistical data deserves recognition, and any future project of this kind should be supported, as it provides a direct opportunity for the different potentials of both institutions to be brought together to discuss and explore advanced statistical, sociological and regional research. The 2018 conference was in fact its third edition, which indicates that the tradition of such a collaboration has already been established and gives hope to be upheld in the future. Despite the delay between the time of the conference and the publication date of the monograph, it still raises highly topical issues, and the presented analytical approaches combine the concern for scientific matters with policy objectives, assuming that the well-being of the citizens is the ultimate goal of community development. A wide spectrum of processes and their relevant factors, analysed at individual and community levels, embraces the elements of social, human and material capital along with innovative forms of their use by various agents, contributing to the cohesion and development of local communities. The variety of methodological approaches used by the authors makes their articles interesting and recommendable to all researchers and decision-makers, as well as to students of the subjects discussed in this publication.
Dostawca treści:
Biblioteka Nauki
Książka
Tytuł:
Engineered resistance against proteinases
Autorzy:
Milner, Malgorzata
Chroboczek, Jadwiga
Zagorski-Ostoja, Wlodzimierz
Powiązania:
https://bibliotekanauki.pl/articles/1040935.pdf
Data publikacji:
2007
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
fusion proteins
proteinase inhibitors
protein protection
Opis:
Exogenous proteinase inhibitors are valuable and economically interesting protective biotechnological tools. We examined whether small proteinase inhibitors when fused to a selected target protein can protect the target from proteolytic degradation without simultaneously affecting the function and activity of the target domain. Two proteinase inhibitors were studied: a Kazal-type silk proteinase inhibitor (SPI2) from Galleria mellonella, and the Cucurbita maxima trypsin inhibitor I (CMTI I). Both inhibitors target serine proteinases, are small proteins with a compact structure stabilized by a network of disulfide bridges, and are expressed as free polypeptides in their natural surroundings. Four constructs were prepared: the gene for either of the inhibitors was ligated to the 5' end of the DNA encoding one or the other of two selected target proteins, the coat protein (CP) of Potato potyvirus Y or the Escherichia coli β-glucuronidase (GUS). CMTI I fused to the target proteins strongly hampered their functions. Moreover, the inhibitory activity of CMTI I was retained only when it was fused to the CP. In contrast, when fused to SPI2, specific features and functions of both target proteins were retained and the inhibitory activity of SPI2 was fully preserved. Measuring proteolysis in the presence or absence of either inhibitor, we demonstrated that proteinase inhibitors can protect target proteins used either free or as a fusion domain. Interestingly, their inhibitory efficiency was superior to that of a commercial inhibitor of serine proteinases, AEBSF.
Źródło:
Acta Biochimica Polonica; 2007, 54, 3; 523-536
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Elements regulating Potato leafroll virus sgRNA1 translation are located within the coding sequences of the coat protein and read-through domain
Autorzy:
Łoniewska-Lwowska, Adrianna
Chełstowska, Sylwia
Zagórski-Ostoja, Włodzimierz
Pałucha, Andrzej
Powiązania:
https://bibliotekanauki.pl/articles/1040473.pdf
Data publikacji:
2009
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
polerovirus
subgenomic RNA
in vitro translation
Opis:
Translation of viral proteins from subgenomic RNAs (sgRNAs) is a common strategy among positive-stranded RNA viruses. Unlike host mRNA, sgRNA of Potato leafroll virus (PLRV) does not possess a cap at its 5' end nor a poly(A) tail at the 3' terminus, both of which are known to be crucial for translation of RNA in eukaryotic cells. Here, we demonstrate, that in wheat germ extract (WGE) truncation of the sgRNA1 5' UTR increases translation efficiency, as it has previously been observed in rabbit reticulocyte lysate (RRL), whereas removal of the 3' UTR does not affect translation. We also describe two regulatory elements located within the coding sequence of the coat protein (CP) gene and its read-through domain (RTD) and are responsible for regulation of in vitro translation of the PLRV sgRNA1. The first element is composed of the purine sequence AAAGGAAA located between the AUG codons of the CP and 17K genes. Deletion of this domain or its substitution by pyrimidines reduced by half the translation of both genes, whereas deletion of the RTD resulted in a 3.6-fold reduction in translation efficiency. This is the first report of translation regulatory elements of plant viruses located within a coding region.
Źródło:
Acta Biochimica Polonica; 2009, 56, 4; 619-625
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Expression, purification and characterization of glycosylated influenza H5N1 hemagglutinin produced in Pichia pastoris
Autorzy:
Kopera, Edyta
Dwornyk, Angela
Kosson, Piotr
Florys, Katarzyna
Sączyńska, Violetta
Dębski, Janusz
Cecuda-Adamczewska, Violetta
Szewczyk, Bogusław
Zagórski-Ostoja, Włodzimierz
Grzelak, Krystyna
Powiązania:
https://bibliotekanauki.pl/articles/1039268.pdf
Data publikacji:
2014
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
Avian influenza
H5N1
recombinant hemagglutinin
Pichia pastoris
Opis:
The A/swan/Poland/305-135V08/2006 (H5N1-subtype) hemagglutinin (HA) gene was cloned and expressed in yeast Pichia pastoris (P. pastoris). The HA cDNA lacking the C-terminal transmembrane anchor-coding sequence was fused to an α-factor leader peptide and placed under control of the methanol-inducible P. pastoris alcohol oxidase 1 (AOX1) promoter. Two P. pastoris strains: SMD 1168 and KM 71 were used for protein expression. Recombinant HA protein was secreted into the culture medium reaching an approximately 15 mg/L (KM 71 strain). Fusion protein with a His6 tag was purified to homogeneity in one step affinity chromatography. SDS-PAGE and MS/MS analysis indicated that the protein is cleaved into HA1 and HA2 domains linked by a disulfide bond. Analysis of the N-linked glycans revealed that the overexpressed HA is fully glycosylated at the same sites as the native HA in the vaccine strain. Immunological activity of the hemagglutinin protein was tested in mice, where rHA elicited a high immune response.
Źródło:
Acta Biochimica Polonica; 2014, 61, 3; 597-602
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Kolejny wielki genom poznany przy udziale polskich laboratoriów: genom ziemniaka zsekwencjonowany
Next eukaryotic genome revealed with cooperation of the Polish laboratories. Potato genome sequenced
Autorzy:
Gromadka, Robert
Gawor, Jan
Szczęsny, Paweł
Zagórski, Włodzimierz
Powiązania:
https://bibliotekanauki.pl/articles/1195370.pdf
Data publikacji:
2011
Wydawca:
Polskie Towarzystwo Przyrodników im. Kopernika
Opis:
W połowie lipca 2011, w Nature ukazał się artykuł "Sekwencja genomu ziemniaka i jej analiza" autorstwa Konsorcjum Sekwencjonowania Genomu Ziemniaka (PGSC). W skład tego Konsorcjum wchodziły 32 zespoły z 14 krajów - znaczących producentów ziemniaków. Współautorami ze strony polskiej, tej wielo autorskiej pracy (94 badaczy, 25 zespołów wiodących) byli członkowie zespołu z Instytutu Biochemii i Biofizyki PAN. Prace Konsorcjum, rozpoczęte w 2007 roku, wspierane były przez rządy państw uczestniczących w programie, w tym Polskiego Ministerstwa Nauki i Szkolnictwa Wyższego, (projekt 47/PGS/2006/01). To trzeci wielki genom (po S. cerevisiae i P. caudatum) w którego poznaniu uczestniczyli pracownicy IBB PAN. Konsorcjum zsekwencjonowało dwa szczepy ziemniaka DM1-3 516 R44 and RH 89-039-16. Szczep DM zsekwencjonowano metodą "shotgun" wykorzystując m. in. platformę sekwencjonowań genomowych IBB PAN (sekwenator Roche GS FLX Titanium 454). Metodami ab initio określono zasób genów kodowanych przez otrzymane sekwencje. Wyniki zweryfikowano analizami transkryptomu objawiającego się w różnych tkankach, czy stadiach rozwojowych oraz w wyniku kontrolowanego stresu. Sumarycznie zidentyfikowano w otrzymanej sekwencji 39031 genów kodujących białka. 25,3% tych genów koduje transkrypty mogące podlegać alternatywnemu składaniu, a więc kontrolujące średnio 2-3 odmienne białka. Wydaje się więc, że w genomie ziemniaka zakodowana jest informacja na temat syntezy ok. 100,000 różnych białek. Porównanie sekwencji DM and RH dowodzi, że ziemniak to roślina wysoce heterozygotyczna. Mutacje punktowe (SNP) występują średnio co 40 nukleotydów, a insercje lub delecje co 394 pary zasad. Wskazuje to wyraźnie, że genom ziemniaka jest niestabilny, co zapewne znajduje swoje odbicie w łatwej degeneracji odmian uprawnych.
In mid-July 2011 Nature published the paper "Genome sequence and analysis of the tuber crop potato" by the Potato Genome Sequencing Consortium - PGSC. This international consortium consisted of 32 teams from 14 countries that are significant potato producers are active in potato breeding programs. The Polish team, representing the PAS Institute of Biochemistry and Biophysics, co-authored the paper, which was signed by 94 consortium members from 25 leading institutions. The work, begun in 2007, was funded by the participating countries' governments - including Poland's Ministry of Science and Higher Education - within the 47/PGS/2006/01. After the yeast and paramecium genomes this is the third large genome sequencing in which IBB teams have participated. Consortium sequenced two strains of potato DM1-3 516 R44 and RH 89-039-16. The DM strain was sequenced by the consortium using the "Shotgun method" on genome sequencing platforms, one of which was created in Warsaw (Roche GS FLX Titanium 454 sequencer).The second strain sequenced was a laboratory S. tuberosum heterodiploid RH 89-039-16. Ab initio predictions of genes and their functions were verified by RNA transcriptome analysis done for various tissues, development stages and under stress. This allowed for the identification of 39031 gene-coding proteins. 25,3% of these genes produce RNA that undergoes splicing, coding for an average of 2-3 different proteins. It therefore seems that the potato genome codes for approximately 100 000 different proteins. Comparisons of the DM and RH sequences show that the potato exhibits high heterozygosity. Single-nucleotiste polymorphisms (SNP) are encountered on average every 40 nucleotides, while insertions or deletions (so-called indel) of an average length of 12.8 nucleotides are encountered on average every 394 base pairs. This data clearly shows that gene damage in the potato genome is a frequent occurrence, which accounts for the easy degeneration of industrial strains.
Źródło:
Kosmos; 2011, 60, 3-4; 491-497
0023-4249
Pojawia się w:
Kosmos
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 16-30 z 34

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