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Wyświetlanie 16-30 z 34
Tytuł:
Cyrano de Bergerac
Autorzy:
Rostand, Edmond
Współwytwórcy:
Zagórski, Włodzimierz
Choromańska, Paulina
Konopnicka, Maria
Kotwica, Wojciech
Kopeć, Aleksandra
Data publikacji:
2019-09-12
Wydawca:
Fundacja Nowoczesna Polska
Tematy:
Komedia
Dramat
Modernizm
Opis:
Publikacja zrealizowana w ramach projektu Wolne Lektury (http://wolnelektury.pl). Dofinansowano ze środków Ministra Kultury i Dziedzictwa Narodowego.
Źródło:
Edmund Rostand, Cyrano de Bergerac, Nakładem Redakcyi "Gazety Polskiej", Warszawa 1898.
Dostawca treści:
Wolne Lektury
Książka
Tytuł:
Isolation of wheat germ ribosomes free of high molecular weight inhibitors of the natural messenger translation
Autorzy:
Rychlik, Wojciech
Matie-Zabala, Minehaha
Zagórski, Włodzimierz
Powiązania:
https://bibliotekanauki.pl/articles/1046229.pdf
Data publikacji:
1979
Wydawca:
Polskie Towarzystwo Biochemiczne
Źródło:
Acta Biochimica Polonica; 1979, 26, 1-2; 115-123
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Antibody response to DNA vaccine against H5N1 avian influenza virus in broilers immunized according to three schedules
Autorzy:
Stachyra, Anna
Góra-Sochacka, Anna
Zagórski-Ostoja, Włodzimierz
Król, Ewelina
Sirko, Agnieszka
Powiązania:
https://bibliotekanauki.pl/articles/1039267.pdf
Data publikacji:
2014
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
Avian influenza
H5N1
DNA vaccine
broilers
Opis:
Broiler type chickens were immunized intramuscularly with a DNA vaccine encoding hemagglutinin (HA) from H5N1 avian influenza virus. The chickens were divided into four groups: control group which was not immunized, a group which obtained only one dose, and two groups which were immunized twice, one group with a boost two weeks after the priming and the other four weeks. Blood samples were collected at several time points and the dynamics of the humoral response to the vaccine was studied. High level of anti-HA antibodies was detected only in the last two groups, that is in chickens immunized according to the prime-boost strategy, regardless of the schedule. An additional interesting observation of this study was detection of the cross-reactivity of an anti-H5 HA positive serum with H5N2 and H1N1 viruses, suggesting that the DNA vaccine tested can induce antibodies of a broad specificity.
Źródło:
Acta Biochimica Polonica; 2014, 61, 3; 593-596
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Virus-like particles of potato leafroll virus as potential carrier system for nucleic acids.
Autorzy:
Sułuja, Elżbieta
Strokowskaja, Ludmiła
Zagórski-Ostoja, Włodzimierz
Pałucha, Andrzej
Powiązania:
https://bibliotekanauki.pl/articles/1041380.pdf
Data publikacji:
2005
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
encapsidation
Virus-like particles
Opis:
Potato leafroll virus is a member of the polerovirus genus. The isometric virion is formed by a coat protein encapsidating single-stranded, positive-sense, mono-partite genomic RNA with covalently attached viral protein at the 5' end. The coat protein of the virus exists in two forms: i) a 23 kDa protein, the product of the coat protein gene, and ii) a 78 kDa protein, the product of the coat protein gene and an additional open reading frame expressed by read-through of the coat protein gene stop codon. The aim of this work was the expression of potato leafroll virus coat protein-based proteins that would be able to assemble into virus-like particles in insect cells. These modified particles were tested for their ability to encapsidate nucleic acids. Two types of N-terminally His-tagged coat protein constructs were used for the expression in insect cells: one, encoding a 23 kDa protein with the C-terminal amino-acid sequence corresponding to the wild type coat protein and the second with additional clathrin binding domain at the C-terminus. The expression of these two proteins by a recombinant baculovirus was characterized by Western immunoblotting with antibodies directed against potato leafroll virus. The protection or putative encapsidation of nucleic acids by these two coat protein derivatives was shown by DNase I and RNase A protection assays.
Źródło:
Acta Biochimica Polonica; 2005, 52, 3; 699-702
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Expression of avian influenza haemagglutinin (H5) and chicken interleukin 2 (chIL-2) under control of the ptcB promoter in Lactococcus lactis
Autorzy:
Szatraj, Katarzyna
Szczepankowska, Agnieszka
Sączyńska, Violetta
Florys, Katarzyna
Gromadzka, Beata
Łepek, Krzysztof
Płucienniczak, Grażyna
Szewczyk, Bogusław
Zagórski-Ostoja, Włodzimierz
Bardowski, Jacek
Powiązania:
https://bibliotekanauki.pl/articles/1039270.pdf
Data publikacji:
2014
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
Lactococcus lactis
ptcB promoter
heterologous gene expression
avian influenza H5N1
H5 haemagglutinin
chicken interleukin-2
Opis:
Gram-positive and nonpathogenic lactic acid bacteria (LAB) are considered to be promising candidates for the development of new, safe systems of heterologous protein expression. Recombinant LAB has been shown to induce specific local and systemic immune response against selected pathogens, and could be a good alternative to classical attenuated carriers. The main goal of our study was to express the avian influenza haemagglutinin (H5) and chicken interleukin 2 (chIL-2) in Lactococcus lactis. Results of this study were anticipated to lead to construction of lactococcal strain(s) with potential vaccine properties against the avian influenza A (H5N1) virus. Expression of the cloned H5 gene, its His-tagged variant and chIL-2 gene, under the control of the ptcB gene promoter was attested by RT-PCR on transcriptional level and Western or dot blot analysis on translational level, demonstrating that system can be an attractive solution for production of heterologous proteins. The results of the preliminary animal trial conducted in mice are a promising step toward development of a vaccine against avian bird flu using Lactococcus lactis cells as antigen carriers.
Źródło:
Acta Biochimica Polonica; 2014, 61, 3; 609-614
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Digoxigenin-labelled molecular probe for the simultaneous detection of three potato pathogens: potato spindle tuber viroid (PSTVd), potato virus Y (PVY), and potato leafroll virus (PLRV)
Autorzy:
Wełnicki, Marek
Żekanowski, Cezary
Zagórski, Włodzimierz
Powiązania:
https://bibliotekanauki.pl/articles/1045314.pdf
Data publikacji:
1994
Wydawca:
Polskie Towarzystwo Biochemiczne
Źródło:
Acta Biochimica Polonica; 1994, 41, 4; 473-475
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Analysis of potyvirus terminal protein VPg-transgenic Arabidopsis thaliana plants
Autorzy:
Wojtal, Izabela
Piontek, Paulina
Grzela, Renata
Jarmołowski, Artur
Zagórski, Włodzimierz
Chroboczek, Jadwiga
Powiązania:
https://bibliotekanauki.pl/articles/1039885.pdf
Data publikacji:
2011
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
VPg protein
Potyvirus
pathogen-derived resistance
transgenic plant
Opis:
Virus-coded VPg protein of Potato virus Y (PVY) does not have homologs apart from other VPgs. Since VPg is indispensable for the potyvirus life cycle, it appeared a good candidate for eliciting pathogen-derived resistance to PVY. Following agroinfection used to obtain PVY VPg-transgenic Arabidopsis thaliana plants, only few transgenic seeds were recovered giving rise to six transgenic plants that contained the VPg gene with the correct sequence. They generated VPg mRNA, but VPg protein was not detected. Some plants were immune to PVY infection suggesting post-transcriptional gene silencing. However, the likely PVY VPg toxicity exerted at an early stage of transformed seeds development precludes its use for engineering pathogen-derived resistance.
Źródło:
Acta Biochimica Polonica; 2011, 58, 3; 349-353
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Piotr Słonimski co-founder of molecular genetics – in memoriam
Autorzy:
Zagórski, Włodzimierz
Powiązania:
https://bibliotekanauki.pl/articles/703689.pdf
Data publikacji:
2009
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Piotr Słonimski
yeast molecular biology
non-mendelian genetics
mosaic genes
genomic
Opis:
Piotr Słonimski, full professor of genetics at University Paris 6 and associate professor on Institute of Biochemistry and Biophysics P.A.S. Born in Warsaw 9.11.1922 died in Paris 25.04.2009. He was full member of French Academy of Science and foreign member of Polish Academy of Sciences, Polish Academy of Arts and Sciences as well as of several others European Academies and dr. hc of many European Universities. He was honored by number of scientific prizes, and was holder of several high decorations, French and Polish. Piotr Słonimski studied medicine during the II World War in Warsaw Underground University, and started after the war his scientific career getting in 1946 his M.D. title at Jagiellonian University in Cracow. Being under occupation the active member of Polish underground and soldier of Warsaw 44 insurrection against Nazis, in Poland incorporated into soviet block he did not escaped the repressions falling on the anti-nazi independence fighters and was arrested by communist secret services. After a few months in prison he was liberated and left Poland for France, were in laboratory of Boris Ephrussi he started his career in CNRS, where from 1971 to 1992 was Director of Centre de Genetique Moleculaire. In line with solid french tradition yeast S. cerevisiae was preferred model of his studies. Here he demonstrated non-standard heritage of mitochondrial traits and become one of co-founders of non-mendelian genetics in Eucatyota. By subtle genetic analysis of selected mitochondrial genes he was first to demonstrate the existence of mosaic genes and pointed towards the regulatory function of information carried on by introns. In nineties Piotr Słonimski was a spiritus movens of European yeast genomics program, acknowledged by special issue of Nature, giving full gene repertoire of the first eucaryotic organism. His scientific interest stayed then in genomes. Here with advanced cryptology methods he was looking for the existence of non-trivial rhythms in coding DNA sequences. His death left in sorrow not only his family but also all the scientist of “yeast community” as well as his comrades in arms from underground and Solidarity movement.
Źródło:
Nauka; 2009, 4
1231-8515
Pojawia się w:
Nauka
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 16-30 z 34

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