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    Wyświetlanie 1-5 z 5
    Tytuł:
    Expression of three diadinoxanthin de-epoxidase genes of Phaeodacylum tricornutum in Escherichia coli Origami b and BL21 strain
    Autorzy:
    Bojko, Monika
    Olchawa-Pajor, Monika
    Tuleja, Urszula
    Kuczyńska, Paulina
    Strzałka, Wojciech
    Latowski, Dariusz
    Strzałka, Kazimierz
    Powiązania:
    https://bibliotekanauki.pl/articles/1039507.pdf
    Data publikacji:
    2013
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    diadinoxanthin cycle
    marine diatoms
    violaxanthin
    de-epoxidation
    Opis:
    In the diadinoxanthin cycle the epoxy group is removed from diadinoxanthin and diatoxanthin is created. This conversion takes place e.g. in diatoms with the involvement of the enzyme diadinoxanthin de-epoxidase. In one of the diatom species, Phaeodactylum tricornutum (CCAP 1055/1 strain with genome sequenced) three de-epoxidase genes (PtVDE, PtVDL1, PtVDL2) have been identified, but only one of them (PtVDE) corresponds to violaxanthin de-epoxidase, an enzyme which is commonly found in higher plants. In these studies, the expression of two de-epoxidase genes of another Phaeodactylum tricornutum strain (UTEX 646), which is commonly used in diatom studies, were obtained in Origami b and BL21 E. coli strains. The molecular masses of the mature proteins are about 49 kDa and 60 kDa, respectively, for VDE and VDL2. Both enzymes are active with violaxanthin as a substrate.
    Źródło:
    Acta Biochimica Polonica; 2013, 60, 4; 857-860
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Violaxanthin and diadinoxanthin de-epoxidation in various model lipid systems
    Autorzy:
    Latowski, Dariusz
    Goss, Reimund
    Bojko, Monika
    Strzałka, Kazimierz
    Powiązania:
    https://bibliotekanauki.pl/articles/1039787.pdf
    Data publikacji:
    2012
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    lipids
    de-epoxidation
    diadinoxanthin cycle
    violaxanthin cycle
    Opis:
    The xanthophyll cycle is an important photoprotective process functioning in plants. One of its forms, the violaxanthin (Vx) cycle, involves interconversion between: Vx, antheraxanthin (Ax) and zeaxanthin (Zx). Another kind of the xanthophyll cycle is the diadinoxanthin (Ddx) cycle in which interconversion between Ddx and diatoxanthin (Dtx) occurs. In this study an information on molecular mechanism and regulation of these two types of the xanthophyll cycle is presented. The influence of lipids on the de-epoxidation of the xanthophyll cycle pigments was investigated, with special focus put on the significance of physical properties of the aggregates formed by inverted lipid micelles, which are necessary for activity of the xanthophyll cycle enzymes. In particular, thickness of the hydrophobic fraction of the aggregates, size of the inverted micelles, suggested by mathematical description of the structures and solubility of Vx and Ddx in various kind of lipids were studied. Obtained results show that the rate of de-epoxidation is strongly dependent on the physicochemical properties of the lipids used. The key role for enzyme activation play non-bilayer lipids and the parameters of inverted micelles such as thickness, fluidity of hydrophobic core and their diameter. The presented results show that MGDG and other non-lamellar lipids like different forms of phosphatidylethanolamine are necessary for the Vx and Ddx de-epoxidation because they provide the three-dimensional structures, which are needed for the binding of de-epoxidases and for the accessibility of Vx and Ddx to these enzymes.
    Źródło:
    Acta Biochimica Polonica; 2012, 59, 1; 101-103
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Determination of epoxides in Escherichia coli expressing recombinant zeaxanthin epoxidase
    Autorzy:
    Kuczynska, P.
    Leskiewicz, S.
    Strzalka, W.
    Olchawa-Pajor, M.
    Bojko, M.
    Latowski, D.
    Strzalka, K.
    Powiązania:
    https://bibliotekanauki.pl/articles/81277.pdf
    Data publikacji:
    2013
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    conference
    zeaxanthin epoxidase
    overexpression
    violaxanthin
    antheraxanthin
    Escherichia coli
    spectrofluorometry
    Źródło:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 3
    0860-7796
    Pojawia się w:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Occurrence of neoxanthin and lutein epoxide cycle in parasitic Cuscuta species
    Autorzy:
    Kruk, Jerzy
    Szymańska, Renata
    Powiązania:
    https://bibliotekanauki.pl/articles/1040836.pdf
    Data publikacji:
    2008
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    violaxanthin de-epoxidase
    Cuscuta
    xanthophyll cycle
    zeaxanthin epoxidase
    neoxanthin
    lutein epoxide cycle
    Opis:
    In the present study, xanthophyll composition of eight parasitic Cuscuta species under different light conditions was investigated. Neoxanthin was not detected in four of the eight species examined, while in others it occurred at the level of several percent of total xanthophylls. In C. gronovii and C. lupuliformis it was additionally found that the neoxanthin content was considerably stimulated by strong light. In dark-adapted plants, lutein epoxide level amounted to 10-22% of total xanthophylls in only three species, the highest being for C. lupuliformis, while in others it was below 3%, indicating that the lutein epoxide cycle is limited to only certain Cuscuta species. The obtained data also indicate that the presence of the lutein epoxide cycle and of neoxanthin is independent and variable among the Cuscuta species. The xanthophyll cycle carotenoids violaxanthin, antheraxanthin and zeaxanthin were identified in all the examined species and occurred at the level found in other higher plants. The xanthophyll and lutein epoxide cycle pigments showed typical response to high light stress. The obtained results also suggest that the ability of higher plants to synthesize lutein epoxide probably does not depend on the substrate specificity of zeaxanthin epoxidase but on the availability of lutein for the enzyme.
    Źródło:
    Acta Biochimica Polonica; 2008, 55, 1; 183-190
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Content of carotenoids in needles of Pinus sylvestris L. growing in a polluted area
    Autorzy:
    Matysiak, R.
    Powiązania:
    https://bibliotekanauki.pl/articles/41281.pdf
    Data publikacji:
    2001
    Wydawca:
    Polska Akademia Nauk. Instytut Dendrologii PAN
    Tematy:
    beta-carotene
    violaxanthin
    polluted area
    carotenoid content
    needle
    neoxanthin
    growing
    Pinus sylvestris
    environment pollution
    lutein
    thin-layer chromatography
    heavy metal
    Opis:
    Carotenoids (neoxanthin, violaxanthin, lutein and β-carotene) present in plant cell play a role in photoprotection. Industrial pollution causes oxidative stress in plants, while carotenoids react with free radicals and dissipate the excess of excitation energy. In this way carotenoids prevent the negative influence of free radicals on metabolism and can even restore some of the damages. This is confirmed by results of our analysis of the level of xanthophylls in 16 and 17-year-old trees of Scots pine (Pinus sylvestris L.). Developed from seed collected in north-eastern, the trees grow in a relatively unpolluted control site, and in a polluted site located 2 km for away from the Phosphorus Fertilizer Works. In the polluted site the environment is contaminated with SO2, NOx and F, Al, F, Pb, Cu. The needles analysed in this study were visually undamaged. Material was collected in experimental plot from 6 trees in October'98 and April'99, between 12.00 and 13.00 hours, at full sunlight. The pigments were analysed quantitatively and qualitatively by thin-layer chromatography. The paper presents results of content and distribution of neoxanthin, violaxanthin, lutein and β-carotene of Scots pine needles from a healthy control tree and a stressed tree. Marked differences in pigment levels depended on the stage of needle development and level of pollution.
    Źródło:
    Dendrobiology; 2001, 46
    1641-1307
    Pojawia się w:
    Dendrobiology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-5 z 5

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