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Wyświetlanie 1-15 z 46
Tytuł:
Lipids and signal transduction in the nucleus.
Autorzy:
Dygas, Anna
Barańska, Jolanta
Powiązania:
https://bibliotekanauki.pl/articles/1044152.pdf
Data publikacji:
2001
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
signal transduction
nucleus
Opis:
During the last few years a growing amount of data has accumulated showing phospholipid participation in nuclear signal transduction. Very recent data strongly support the hypothesis that signal transduction in the nucleus is autonomic. Local production of inositol polyphosphates, beginning with the activation of phospholipase C is required for their specific function in the nucleus. Enzymes which modify polyphosphoinositols may control gene expression. Much less information is available about the role of other lipids in nuclear signal transduction. The aim of this minireview is to stress what is currently known about nuclear lipids with respect to nuclear signal transduction.
Źródło:
Acta Biochimica Polonica; 2001, 48, 2; 541-549
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Hydrolysis of cyclic GMP in rat peritoneal macrophages.
Autorzy:
Witwicka, Hanna
Kobiałka, Marcin
Gorczyca, Wojciech
Powiązania:
https://bibliotekanauki.pl/articles/1043691.pdf
Data publikacji:
2002
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
signal transduction
phosphodiesterases
cyclic nucleotides
macrophages
Opis:
Intact rat peritoneal macrophages (rPM) treated with 3-isobutyl-1-methylxanthine (IBMX), an inhibitor of phosphodiesterases (PDEs), accumulated more cGMP than untreated cells. A PDE activity toward [3H]cGMP was detected in the soluble and particulate fractions of rPM. The hydrolysis of cGMP was Ca2+/calmodulin-independent but increased in the presence of cGMP excess. Similar results were obtained when [3H]cAMP was used as a substrate. The hydrolytic activity towards both nucleotides was inhibited in the presence of IBMX. Therefore, the PDEs of families 2, 5, 10 and 11 are potential candidates for cGMP hydrolysis in the rPM. They may not only regulate the cGMP level in a feedback-controlled way but also link cGMP-dependent pathways with those regulated by cAMP.
Źródło:
Acta Biochimica Polonica; 2002, 49, 4; 891-897
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Optimization of a retroviral vector for transduction of human CD34 positive cells
Autorzy:
Szyda, Anna
Paprocka, Maria
Krawczenko, Agnieszka
Lenart, Katarzyna
Heimrath, Jerzy
Grabarczyk, Piotr
Mackiewicz, Andrzej
Duś, Danuta
Powiązania:
https://bibliotekanauki.pl/articles/1041181.pdf
Data publikacji:
2006
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
transduction optimization
retroviral vector
CD34+ cells
Opis:
Human stem and progenitor cells have recently become objects of intensive studies as an important target for gene therapy and regenerative medicine. Retroviral vectors are among the most effective tools for genetic modification of these cells. However, their transduction efficiency strongly depends on the choice of the ex vivo transduction system. The aim of this study was to elaborate a system for retroviral vector transduction of human CD34 positive cells isolated from cord blood. The retroviral vector pMINV EGFP was chosen for transduction of two human erythroblastoid cell lines: KG-1a (CD34 positive) and K562 (CD34 negative). For vector construction, three promoters and two retroviral vector packaging cell lines were used. To optimize the physicochemical conditions of the transduction process, different temperatures of supernatant harvesting, the influence of centrifugation and the presence of transduction enhancing agents were tested. The conditions elaborated with KG-1a cells were further applied for transduction of CD34 positive cells isolated from cord blood. The optimal efficiency of transduction of CD34 positive cells with pMINV EGFP retroviral vector (26% of EGFP positive cells), was obtained using infective vector with LTR retroviral promoter, produced by TE FLY GA MINV EGFP packaging cell line. The transduction was performed in the presence of serum, at 37°C, with co-centrifugation of cells with viral supernatants and the use of transduction enhancing agents. This study confirmed that for gene transfer into CD34 positive cells, the detailed optimization of each element of the transduction process is of great importance.
Źródło:
Acta Biochimica Polonica; 2006, 53, 4; 815-823
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The basic biology of erbB-2 and its participation in colorectal cancers
Autorzy:
Stawińska, Magdalena
Bryś, Magdalena
Krajewska, Wanda M
Powiązania:
https://bibliotekanauki.pl/articles/764806.pdf
Data publikacji:
2005
Wydawca:
Uniwersytet Łódzki. Wydawnictwo Uniwersytetu Łódzkiego
Tematy:
ErbB-2
colorectal cancers
signal transduction
tumour growth
Opis:
ErbB-2 is one of Tour cell surface growth factor receptors involved in transmission of signals controlling normal cell growth and differentiation. A range of growth factors serve as ligands, but none is specific for the ErbB-2 receptor. Ligand binding to ErbB-1, ErbB-3 and ErbB-4 induces rapid receptor dimerization, with a marked preference for ErbB-2 as a dimer partner. When ErbB-2 is overexpressed multiple ErbB-2 heterodimers are formed and cell signalling is stronger, resulting in enhanced responsiveness to growth factors and malignant growth. This explains why ErbB-2 overexpression is an indicator of poor prognosis in colorectal cancers and may be predictive of response to treatment. ErbB-2 is a highly specific and promising target for new colon cancer treatments.
Źródło:
Acta Universitatis Lodziensis. Folia Biologica et Oecologica; 2005, 2
1730-2366
2083-8484
Pojawia się w:
Acta Universitatis Lodziensis. Folia Biologica et Oecologica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Signal transmission via G protein-coupled receptors in the light of rhodopsin structure determination.
Autorzy:
Ciarkowski, Jerzy
Drabik, Piotr
Giełdoń, Artur
Kaźmierkiewicz, Rajmund
Ślusarz, Rafał
Powiązania:
https://bibliotekanauki.pl/articles/1044085.pdf
Data publikacji:
2001
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
signal transduction
neurohypophyseal receptors
molecular modeling
GPCRs
Opis:
G protein-coupled receptors (GPCRs) transducing diverse external signals to cells via activation of heterotrimeric GTP-binding (G) proteins, estimated to mediate actions of 60% of drugs, had been resistant to structure determination until summer 2000. The first atomic-resolution experimental structure of a GPCR, that of dark (inactive) rhodopsin, thus provides a trustworthy 3D prototype for antagonist-bound forms of this huge family of proteins. In this work, our former theoretical GPCR models are evaluated against the new experimental template. Subsequently, a working hypothesis regarding the signal transduction mechanism by GPCRs is presented.
Źródło:
Acta Biochimica Polonica; 2001, 48, 4; 1203-1207
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Slowing down aging from within: mechanistic aspects of anti-aging hormetic effects of mild heat stress on human cells.
Autorzy:
Rattan, Suresh
Gonzalez-Dosal, Regina
Nielsen, Elise
Kraft, David
Weibel, Jens
Kahns, Søren
Powiązania:
https://bibliotekanauki.pl/articles/1043285.pdf
Data publikacji:
2004
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
aging
signal transduction
anti-aging
longevity
proteasome
heat shock
Opis:
Since aging is primarily the result of a failure of maintenance and repair mechanisms, various approaches are being developed in order to stimulate these pathways and modulate the process of aging. One such approach, termed hormesis, involves challenging cells and organisms by mild stress that often results in anti-aging and life prolonging effects. In a series of experimental studies, we have reported that repeated mild heat stress (RMHS) has anti-aging hormetic effects on growth and various cellular and biochemical characteristics of human skin fibroblasts undergoing aging in vitro. These beneficial effects of repeated challenge include the maintenance of stress protein profile, reduction in the accumulation of oxidatively and glycoxidatively damaged proteins, stimulation of the proteasomal activities for the degradation of abnormal proteins, improved cellular resistance to other stresses, and enhanced levels of cellular antioxidant ability. In order to elucidate the molecular mechanisms of hormetic effects of RMHS, we are now undertaking studies on signal transduction pathways, energy production and utilisation kinetics, and the proteomic analysis of patterns of proteins synthesised and their posttranslational modifications in various types of human cells undergoing cellular aging in vitro. Human applications of hormesis include early intervention and modulation of the aging process to prevent or delay the onset of age-related conditions, such as sarcopenia, Alzheimer's disease, Parkinson's disease, cataracts and osteoporosis.
Źródło:
Acta Biochimica Polonica; 2004, 51, 2; 481-492
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Certain protein transducing agents convert translocated proteins into cell killers
Autorzy:
Tcherniuk, Siergiej
Fiser, Anne-Laure
Derouazi, Madiha
Toussaint, Bertrand
Wang, Yan
Wojtal, Izabela
Kondo, Eisaku
Szolajska, Ewa
Chroboczek, Jadwiga
Powiązania:
https://bibliotekanauki.pl/articles/1039725.pdf
Data publikacji:
2012
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
cytotoxic protein
cell transduction
protein delivery
cell translocation
transducing peptides
Opis:
The majority of proteins are unable to translocate into the cell interior. Hence for peptide- and protein-based therapeutics a direct intracytoplasmic delivery with the aid of transducing agents is an attractive approach. We wanted to deliver to the cell interior a putatively cytotoxic protein VPg. Protein transduction was achieved in vitro with three different commercial products. However, in our hands, delivery of various control proteins without known deleterious effects, as well as of protein VPg, always induced cell death. Finally, we used a novel transducing peptide Wr-T, which was not toxic to cultured cells, even in a quite large range of concentrations. Most importantly, control protein delivered to cells in culture did not display any toxicity while VPg protein exerted a strong cytotoxic effect. These data show that results obtained with cell-penetrating agents should be interpreted with caution.
Źródło:
Acta Biochimica Polonica; 2012, 59, 3; 433-439
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Structure and functions of plant calcium-dependent protein kinases
Autorzy:
Klimecka, Maria
Muszyńska, Grażyna
Powiązania:
https://bibliotekanauki.pl/articles/1041065.pdf
Data publikacji:
2007
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
signal transduction
stress responses
cross-talk
calcium
plant protein kinases
Opis:
Calcium ions as second messengers play an essential role in many important cellular processes. In plants, transient changes in calcium content in the cytosol (calcium signatures) have been observed during growth, development and under stress conditions. Such diverse functions require many different calcium sensors. One of the largest and most differentiated group of calcium sensors are protein kinases, among them calcium-dependent protein kinases (CDPKs) which were identified only in plants and protists. CDPKs have a regulatory domain which is able to bind calcium ions. For regulation of CDPKs activities not only calcium ions but also specific phospholipids and autophosphorylation are responsible. CDPKs have many different substrates, which reflects the diversity of their functions. Potential protein substrates of CDPK are involved in carbon and nitrogen metabolism, phospholipid synthesis, defense responses, ion and water transport, cytoskeleton organization, transcription and hormone responses. Presently, participation of CDPKs in stress signal transduction pathways (e.g., cold, drought, high salinity, wounding) is intensively studied in many laboratories. An intriguing, but still not fully clarified problem is the cross-talk via CDPKs among different signaling pathways that enables signal integration at different levels and ensure appropriate downstream responses.
Źródło:
Acta Biochimica Polonica; 2007, 54, 2; 219-233
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Molecular cloning, characterization and expression of a guanylyl cyclase (HpGC1) involved in the stress signalling in Hippeastrum x hybr.
Autorzy:
Swiezawska, B.
Szewczuk, P.
Pawelek, A.
Jaworski, K.
Szmid-Jaworska, A.
Powiązania:
https://bibliotekanauki.pl/articles/81222.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
conference
molecular cloning
expression
characteristics
guanylyl cyclase
signal transduction
light signal transduction
biotic stress
abiotic stress
stress tolerance
Hippeastrum x hybridum
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 3
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of aluminium on plant growth and metabolism.
Autorzy:
Mossor-Pietraszewska, Teresa
Powiązania:
https://bibliotekanauki.pl/articles/1044097.pdf
Data publikacji:
2001
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
aluminium
signal transduction pathway
phytotoxicity
plant response
oxidative stress
Opis:
Aluminium toxicity is one of the major factors that limit plant growth and development in many acid soils. Root cells plasma membrane, particularly of the root apex, seems to be a major target of Al toxicity. However, strong interaction of Al3+, the main Al toxic form, with oxygen donor ligands (proteins, nucleic acids, polysaccharides) results in the inhibition of cell division, cell extension, and transport. Although the identification of Al tolerance genes is under way, the mechanism of their expression remains obscure.Soil chemical factors that limit root growth in acid soils, diminish crop production, include Al, Mn and various cations, and also deficiency or unavailability of Ca, Mg, P, Mo, and Si. These effects are further complicated by interactions of Al with other ions in different plant genotypes and under stress conditions (Foy, 1992). Cytotoxicity of Al has been well documented in plants (Delhaize & Ryan, 1995; Horst et al., 1999; Kollmeier et al., 2000; Marienfeld et al., 2000). It is generally known that plants grown in acid soils due to Al solubility at low pH have reduced root systems and exhibit a variety of nutrient-deficiency symptoms, with a consequent decrease in yield. In many countries with naturally acid soils, which constitute about 40% of world arable soil (LeNoble et al., 1996), Al toxicity is a major agricultural problem, and is intensively studied in plant systems.The effects of aluminium on plant growth, crop yield, uptake and nutrients distribution in vegetative and reproductive parts are still not fully understood. This review discusses recent information on aluminium toxicity with an emphasis on plant response to Al stress.
Źródło:
Acta Biochimica Polonica; 2001, 48, 3; 673-686
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Particulate guanylyl cyclases: multiple mechanisms of activation.
Autorzy:
Kobiałka, Marcin
Gorczyca, Wojciech
Powiązania:
https://bibliotekanauki.pl/articles/1044281.pdf
Data publikacji:
2000
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
signal transduction
calcium-binding proteins
guanylyl cyclases
cyclic nucleotides
Opis:
Cyclic GMP (cGMP), a key messenger in several signal transduction pathways, is synthesized from GTP by a family of enzymes termed guanylyl cyclases, which are found in two forms: cytosolic (soluble) and membrane-bound (particulate). The past decade has brought significant progress in understanding the molecular mechanisms that underlie the regulation of particulate guanylyl cyclases and new members of their family have been identified. It has become more evident that the basic mechanism of catalysis of guanylyl cyclases is analogous to that recognized in adenylyl cyclases. Here we review the known basic mechanisms that contribute to the regulation of particulate guanylyl cyclases.
Źródło:
Acta Biochimica Polonica; 2000, 47, 3; 517-528
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Arabidopsis thaliana MKKK18 is involved in abscisic acid signalling
Autorzy:
Mitula, F.
Tajdel, M.
Sadowski, J.
Ludwikow, A.
Powiązania:
https://bibliotekanauki.pl/articles/81146.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
conference
Arabidopsis thaliana
abscisic acid
MAP kinase inhibitor
signal transduction
gfp gene
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 2
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Development of novel cellular model for affinity studies of histamine H4 receptor ligands
Autorzy:
Karcz, Tadeusz
Kieć-Kononowicz, Katarzyna
Powiązania:
https://bibliotekanauki.pl/articles/1039499.pdf
Data publikacji:
2013
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
retroviral transduction system
histamine H4 receptor
radioligand binding studies
recombinant proteins
Opis:
The G protein-coupled histamine H4 receptor (H4R) is the last member of histamine receptors family discovered so far. Its expression pattern, together with postulated involvement in a wide variety of immunological and inflammatory processes make histamine H4 receptor an interesting target for drug development. Potential H4R ligands may provide an innovative therapies for different immuno-based diseases, including allergy, asthma, pruritus associated with allergy or autoimmune skin conditions, rheumatoid arthritis and pain. However, none of successfully developed selective and potent histamine H4 receptor ligands have been introduced to the market up to date. For that reason there is still a strong demand for pharmacological models to be used in studies on potent H4R ligands. In current work we present the development of novel mammalian cell line, stably expressing human histamine H4 receptor, with use of retroviral transduction approach. Obtained cell line was pharmacologically characterized in radioligand binding studies and its utility for affinity testing of potent receptor ligands was confirmed in comparative studies with the use of relevant insect cells expression model. Obtained results allow for statement that developed cellular model may be successfully employed in search for new compounds active at histamine H4 receptor.
Źródło:
Acta Biochimica Polonica; 2013, 60, 4; 823-827
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Learning cross-lingual phonological and orthographic adaptations : a case study in improving neural machine translation between low-resource languages
Autorzy:
Jha, Saurav
Sudhakar, Akhilesh
Singh, Anil Kumar
Powiązania:
https://bibliotekanauki.pl/articles/103899.pdf
Data publikacji:
2019
Wydawca:
Polska Akademia Nauk. Instytut Podstaw Informatyki PAN
Tematy:
neural machine translation
Hindi
Bhojpuri
word transduction
low resource language
attention model
Opis:
Out-of-vocabulary (OOV) words can pose serious challenges for machine translation (MT) tasks, and in particular, for low-resource language (LRL) pairs, i.e., language pairs for which few or no paralel corpora exist. Our work adapts variants of seq2seq models to perform transduction of such words from Hindi to Bhojpuri (an LRL instance), learning from a set of cognate pairs built from a bilingual dictionary of Hindi-Bhojpuri words. We demonstrate that our models can be effectively used for language pairs that have limited paralel corpora; our models work at the character level to grasp phonetic and orthographic similarities across multiple types of word adaptations, whether synchronic or diachronic, loan words or cognates. We describe the training aspects of several character level NMT systems that we adapted to this task and characterize their typical errors. Our method improves BLEU score by 6.3 on the Hindi-to-Bhojpuri translation task. Further, we show that such transductions can generalize well to other languages by applying it successfully to Hindi-Bangla cognate pairs. Our work can be seen as an important step in the process of: (i) resolving the OOV words problem arising in MT tasks; (ii) creating effective parallel corpora for resource constrained languages; and (iii) leveraging the enhanced semantic knowledge captured by word-level embeddings to perform character-level tasks.
Źródło:
Journal of Language Modelling; 2019, 7, 2; 101-142
2299-856X
2299-8470
Pojawia się w:
Journal of Language Modelling
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
A logical approach to grammar description
Autorzy:
Clément, L.
Kirman, J.
Salvati, S.
Powiązania:
https://bibliotekanauki.pl/articles/103895.pdf
Data publikacji:
2015
Wydawca:
Polska Akademia Nauk. Instytut Podstaw Informatyki PAN
Tematy:
grammar description
logic
Finite State Automata
logical transduction
lambda calculus
Abstract Categorial Grammars
Opis:
In the tradition of Model Theoretic Syntax, we propose a logical approach to the description of grammars. We combine in one formalism several tools that are used throughout computer science for their power of abstraction: logic and lambda calculus. We propose then a high-level formalism for describing mildly context sensitive grammars and their semantic interpretation. As we rely on the correspondence between logic and finite state automata, our method combines conciseness with effectivity. We illustrate our approach with a simple linguistic model of several interleaved linguistic phenomena involving extraction. The level of abstraction provided by logic and lambda calculus allows us not only to use this linguistic model for several languages, namely English, German, and Dutch, but also for semantic interpretation.
Źródło:
Journal of Language Modelling; 2015, 3, 1; 87-143
2299-856X
2299-8470
Pojawia się w:
Journal of Language Modelling
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-15 z 46

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