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Wyświetlanie 1-13 z 13
Tytuł:
Activity of specific IgG, IgM and IgE antibodies in human trichinellosis
Aktywność swoistych przeciwciał IgG, IgM i IgE we włośnicy człowieka
Autorzy:
Tomasik, J.
Grzybowski, J.
Golińska, Z.
Prokopowicz, D.
Powiązania:
https://bibliotekanauki.pl/articles/2152263.pdf
Data publikacji:
1992
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
wlosnica
choroby inwazyjne
przeciwciala swoiste
parazytologia
immunologia
aktywnosc immunologiczna
pasozyty czlowieka
Opis:
The activity of IgG, IgM and IgE antibodies to somatic antigen of Trichinella spiralis in the sera of patients with trichinellosis at various intervals after infection was examined by means of ELISA. Mathematical analysis of the dose-response curves was used. Elevated level of IgG and IgM antibodies of relatively high avidity and of rather low IgE avidity was documented. Amount or avidity ofIgG antibodies was found to be most useful for the diagnosis of trichinellosis (85% positive results in patients' sera). The isotype of IgM avidity constitutes a better diagnostic value than the amount of it (60% and 35% of positive results, respectively).
Źródło:
Wiadomości Parazytologiczne; 1992, 38, 3-4; 127-133
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Activity of specific IgG, IgM and IgE antibodies in human trichinellosis
Aktywnosc swoistych przeciwcial IgG, IgM i IgE we wlosnicy czlowieka
Autorzy:
Tomasik, J
Grzybowski, J.
Golinska, Z.
Prokopowicz, D.
Powiązania:
https://bibliotekanauki.pl/articles/841299.pdf
Data publikacji:
1992
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
wlosnica
choroby inwazyjne
przeciwciala swoiste
parazytologia
immunologia
aktywnosc immunologiczna
pasozyty czlowieka
Opis:
The activity of IgG, IgM and IgE antibodies to somatic antigen of Trichinella spiralis in the sera of patients with trichinellosis at various intervals after infection was examined by means of ELISA. Mathematical analysis of the dose-response curves was used. Elevated level of IgG and IgM antibodies of relatively high avidity and of rather low IgE avidity was documented. Amount or avidity ofIgG antibodies was found to be most useful for the diagnosis of trichinellosis (85% positive results in patients' sera). The isotype of IgM avidity constitutes a better diagnostic value than the amount of it (60% and 35% of positive results, respectively).
Źródło:
Annals of Parasitology; 1992, 38, 3-4; 127-133
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Zastosowanie awidnosci swoistych przeciwcial IgG dla roznicowania aktywnego i przewleklego okresu zarazenia Toxocara canis
Autorzy:
Rychlicki, W.
Powiązania:
https://bibliotekanauki.pl/articles/837130.pdf
Data publikacji:
2004
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
Toxocara canis
toksokaroza
pomiary awidnosci
choroby pasozytnicze
przeciwciala swoiste
parazytologia
przeciwciala IgG
diagnostyka
infekcja pasozytnicza
Źródło:
Annals of Parasitology; 2004, 50, 2; 229-236
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Zastosowanie awidności swoistych przeciwciał IgG dla różnicowania aktywnego i przewlekłego okresu zarażenia Toxocara canis
Use of specific immunoglobulin G antibody avidity in the differential diagnosis of active and chronic Toxocara canis infections.
Autorzy:
Rychlicki, W.
Powiązania:
https://bibliotekanauki.pl/articles/2146457.pdf
Data publikacji:
2004
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
Toxocara canis
toksokaroza
pomiary awidnosci
choroby pasozytnicze
przeciwciala swoiste
parazytologia
przeciwciala IgG
diagnostyka
infekcja pasozytnicza
Opis:
To differentiate between acute and past Toxocara canis infections, a modified enzyme-linked immunosorbent assay, based on the dissociation of antigen-antibody complex with 6 M urea solution was used to measure levels of avidity of specific immunoglobulin G antibody against Toxocara canis excretory-secretory antigen. IgG avidity index was determined in the sera of 212 patients, divided into two groups with various stages of Toxocara infection. Patients were classified according to time period, from the onset of clinical symptoms or first positive serology result. Low IgG avidity index was found in the sera of 6.6% of patients in the acute phase, and all samples from the chronic stage of infection were characterized by high avidity values above 0.5 (mean 0.74). It was documented, that the avidity test for specific IgG antibodies is a valuable diagnostic method that helps to distinguish the early from the later phase of Toxocara canis infection. Indications for anti-parasitic treatment according to immunological activity of Toxocara infection were discussed.
Źródło:
Wiadomości Parazytologiczne; 2004, 50, 2; 229-236
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Analiza stezen sIgA w tresci kanalu szyjki macicy i jamy ustnej u kobiet z Candida lub bez grzybow w ontocenozach pochwy i jamy ustnej
Autorzy:
Kurnatowska, A
Magnowski, J.
Powiązania:
https://bibliotekanauki.pl/articles/838699.pdf
Data publikacji:
2002
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
stezenie immunoglobulin
pochwa
grzyby
przeciwciala swoiste
szyjka macicy
Candida
parazytologia
jama ustna
immunoglobulina A
kobiety
Opis:
Analysis of sIgA concentrations in the contents of the cervical canal of the uterus and of the oral cavily in women with Candida or without fungi in ontocenoses of these organs. The aim of the study was to search for fungi in ontocenoses of genital organs and oral cavity (the fungal reservoir for multifocal infections) in women; evaluation of the concentration of sIgA in the contents of the cervical canal of the uterus and of the oral cavity. 102 women (age: 18- 35 years) were examined. Fungi were isolated from ontocenoses of the vagina and the oral cavity; axenic strains were differentiated with API 20 C and API 20 C AUX tests (bioMerieux). The concentrations of sIgA in the content of the cervical canal of the uterus and from the oral cavity were evaluated by LC-Partigen IgA (Behring) tests. Candida occurence in the oral cavity was significant (p<0,02) higher than in the vagina. Candida albicans (6 codes) was the predominat species; there were also C. tropicalis, C. kefyr, C. krusei, C. guilliermondii and C. glabrata. There were no significant differences between sIgA concentrations and the presence or absence of fungi in the vagina or oral cavity.
Źródło:
Annals of Parasitology; 2002, 48, 3; 271-276
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Analiza stężeń sIgA w treści kanału szyjki macicy i jamy ustnej u kobiet z Candida lub bez grzybów w ontocenozach pochwy i jamy ustnej
Autorzy:
Kurnatowska, A.
Magnowski, J.
Powiązania:
https://bibliotekanauki.pl/articles/2147733.pdf
Data publikacji:
2002
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
stezenie immunoglobulin
pochwa
grzyby
przeciwciala swoiste
szyjka macicy
Candida
parazytologia
jama ustna
immunoglobulina A
kobiety
Opis:
Analysis of sIgA concentrations in the contents of the cervical canal of the uterus and of the oral cavily in women with Candida or without fungi in ontocenoses of these organs. The aim of the study was to search for fungi in ontocenoses of genital organs and oral cavity (the fungal reservoir for multifocal infections) in women; evaluation of the concentration of sIgA in the contents of the cervical canal of the uterus and of the oral cavity. 102 women (age: 18- 35 years) were examined. Fungi were isolated from ontocenoses of the vagina and the oral cavity; axenic strains were differentiated with API 20 C and API 20 C AUX tests (bioMerieux). The concentrations of sIgA in the content of the cervical canal of the uterus and from the oral cavity were evaluated by LC-Partigen IgA (Behring) tests. Candida occurence in the oral cavity was significant (p<0,02) higher than in the vagina. Candida albicans (6 codes) was the predominat species; there were also C. tropicalis, C. kefyr, C. krusei, C. guilliermondii and C. glabrata. There were no significant differences between sIgA concentrations and the presence or absence of fungi in the vagina or oral cavity.
Źródło:
Wiadomości Parazytologiczne; 2002, 48, 3; 271-276
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Ocena porownawczego profilu immunologicznego swoistych przeciwcial w plynie srodocznym i krwi obwodowej pacjentow podejrzanych o toksoplazmoze lub toksokaroze oczna
Autorzy:
Paul, M.
Stefaniak, J.
Pecold, K.
Twardosz-Pawlik, H.
Powiązania:
https://bibliotekanauki.pl/articles/838308.pdf
Data publikacji:
2004
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
toksoplazmoza
badania porownawcze
przeciwciala swoiste
toksokaroza oczna
parazytologia lekarska
pacjenci
profil immunologiczny
plyn srodoczny
krew obwodowa
Źródło:
Annals of Parasitology; 2004, 50, 3; 425-433
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Ocena porównawczego profilu immunologicznego swoistych przeciwciał w płynie śródocznym i krwi obwodowej pacjentów podejrzanych o toksoplazmozę lub toksokarozę oczną
Analysis of comparative immunological profiles of intraocular fluid and serum samples in patients suspected of ocular toxoplasmosis or toxocarosis.
Autorzy:
Paul, M.
Stefaniak, J.
Pecold, K.
Twardosz-Pawlik, H.
Powiązania:
https://bibliotekanauki.pl/articles/2147389.pdf
Data publikacji:
2004
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
toksoplazmoza
badania porownawcze
przeciwciala swoiste
toksokaroza oczna
parazytologia lekarska
pacjenci
profil immunologiczny
plyn srodoczny
krew obwodowa
Opis:
High seroprevalence of Toxoplasma and Toxocara spp. in populations of children and adults in Poland constitutes a significant risk of supradiagnosed parasitic eye infections. We described the clinical characteristics in relation to the analysis of comparative immunological profiles of T. gondii-specific antibodies in aqueous humour and serum samples in patients with reactivated retinochoroiditis, and of Toxocara spp. ones in cases with posterior granuloma, fibrotic and calcified tumor-like masses simulating retinoblastoma, detected by ophthalmoscopy and echography. Intraocular synthesis of specific IgG antibodies was detected in anterior eye chamber fluid in 1/2 and 2/3 of patients respectively, strongly suspected of ocular toxoplasmosis or toxocarosis. The evidence of a local production of specific antibodies in intraocular fluid shown by the Western blot seems to be a valuable immunodiagnostic method for a final confirmation of eye lesions of parasitic origin and crucial in the choice of an appropriate treatment regimen.
Źródło:
Wiadomości Parazytologiczne; 2004, 50, 3; 425-433
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Znaczenie kliniczne oznaczania awidnosci swoistych przeciwcial IgG w bablowicy watroby wywolanej przez Echinoccocus granulosus
Autorzy:
Stefaniak, J
Kacprzak, E.
Paul, M.
Powiązania:
https://bibliotekanauki.pl/articles/840892.pdf
Data publikacji:
2001
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
immunoglobulina G
choroby pasozytnicze
przeciwciala swoiste
parazytologia lekarska
badania kliniczne
pasozyty
Echinococcus granulosus
poziom awidnosci
bablowica watroby
Opis:
Role of E. granulosus-specific IgG antibody avidity in evaluation of a natural history of the parasite cysts in the liver, duration of infection and immunogenic cysts activity was studied. Thirty-one patients with liver cysts suspected of echinococcosis were examined; E. Granulosus infection was finally confirmed by a fine needle aspiration biopsy or surgery in 29 cases (93.5%) and two non-parasitic simple cysts in the liver were diagnosed. E. granulosus-specific IgG antibody was found in 21 patients (67.7%). High IgG avidity values were observed in 14 patients with liver cysts in a degcneration stage confirmed by imaging techniques. Low IgG avidity values were mostly detected in children with hypoechogenic echinococcal cysts being in a recent stage of infection. Determination of anti-E. Granulosus immunoglobulin G antibody avidity is a very usefull diagnostic tool for a determination of parasitic cysts activity and their developmental age.
Źródło:
Annals of Parasitology; 2001, 47, 3; 477-482
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Znaczenie kliniczne oznaczania awidności swoistych przeciwciał IgG w bąblowicy wątroby wywołanej przez Echinoccocus granulosus
Autorzy:
Stefaniak, J.
Kacprzak, E.
Paul, M.
Powiązania:
https://bibliotekanauki.pl/articles/2148038.pdf
Data publikacji:
2001
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
immunoglobulina G
choroby pasozytnicze
przeciwciala swoiste
parazytologia lekarska
badania kliniczne
pasozyty
Echinococcus granulosus
poziom awidnosci
bablowica watroby
Opis:
Role of E. granulosus-specific IgG antibody avidity in evaluation of a natural history of the parasite cysts in the liver, duration of infection and immunogenic cysts activity was studied. Thirty-one patients with liver cysts suspected of echinococcosis were examined; E. Granulosus infection was finally confirmed by a fine needle aspiration biopsy or surgery in 29 cases (93.5%) and two non-parasitic simple cysts in the liver were diagnosed. E. granulosus-specific IgG antibody was found in 21 patients (67.7%). High IgG avidity values were observed in 14 patients with liver cysts in a degcneration stage confirmed by imaging techniques. Low IgG avidity values were mostly detected in children with hypoechogenic echinococcal cysts being in a recent stage of infection. Determination of anti-E. Granulosus immunoglobulin G antibody avidity is a very usefull diagnostic tool for a determination of parasitic cysts activity and their developmental age.
Źródło:
Wiadomości Parazytologiczne; 2001, 47, 3; 477-482
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Zastosowanie metody ISAGA w wykrywaniu swoistych przeciwcial IgM, IgA, IgE w nabytej i wrodzonej toksoplazmozie
Autorzy:
Paul, M
Powiązania:
https://bibliotekanauki.pl/articles/839324.pdf
Data publikacji:
1997
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
toksoplazmoza
choroby pasozytnicze
przeciwciala swoiste
metody serologiczne
immunoglobulina M
parazytologia
immunoglobulina A
immunoglobulina E
metoda ISAGA
wykrywanie
Toxoplasma gondii
pasozyty czlowieka
Opis:
Immunocapture assays ISAGA PLUS IgA/IgM (bioMerieux) and IgE ISAGA were used to determine their usefulness in the diagnosis of acquired and congenital toxoplasmosis. Specific IgM, IgA and IgE antibodies were tested in 134 patients, namely pregnant women who seroconverted during gestation (n= 20), children with congenital toxoplasmosis (n= 5), patients with toxoplasmic lymphadenitis (n= 56) and immunocompetent individuals with chronic Toxoplasma gondii infection (n= 53). Altogether 172 sera were examined. Specific IgM antibodies were detected in all sera from pregnant women (100%) with recent T. gondii infection (1- 8 weeks after seroconversion), in all patients with toxoplasmic lymphadenopathy (1-3 months after onset of symptoms) and in their control examinations after 2 and 5 months (100%) and also in 35 (66%) out of 53 patients with chronic infection. In infants with congenital toxoplasmosis IgM were found only in one new-born; equivocal results were obtained in 3 children during the asymptomatic serological reactivation in the second year of life. Specific IgA antibodies were present in sera from 15 (75%) out of 20 women seroconverted during pregnancy; in 3 cases the results were equivocal. IgA antibodies were detected in sera from 30 (81.1%) out of 37 patients with toxoplasmic lymphadenitis examined once; in 19 patients examined 3 times IgA antibodies were present in all the cases in the first serological examination performed when clinical symptoms were first observed (100%), in 17 patients after 2 months (89.5%) and in 11 patients after 5 months (57.9%). IgA antibodies were also detected in 21 sera (39.6%) from patients with chronic T. gondii infection. In children with congenital toxoplasmosis IgA antibodies were found in 3 cases during serological reactivation after discontinuation of pyrimethamine-sulfadiazine therapy; in these cases equivocal results of IgM antibodies were present, and positive result of IgE antibodies in one case. Specific IgE antibodies were detected in sera from 17 (85%) out of 20 women with seroconversion and in 18 patients with lymphadenopathy (32.1%); in the last group IgE antibodies were not present in the follow-up examination after 5 months. IgE antibodies were detected only in 5 cases (9.4%) with chronic infection. IgA and IgE antibodies in ISAGA begin to appear about a week later than IgM antibodies; in sera collected between the 2nd and 3rd week after invasion the positive results were obtained in all cases (100%). Therefore, ISAGA PLUS IgA/IgM (bioMerieux) is useful for the diagnosis of recent T. gondii infection especially in women with suspected seroconversion during pregnancy. ISAGA PLUS IgA/IgM is more sensitive than any conventional method routinely used and so far is a specially eflicient technique for newborns and infants suspected for congenital infection and/or in diagnosing congenital toxoplasmosis during immunological recrudescence. This test has a limited value in toxoplasmosis with lymphadenopathy by reason of possibility of a long persistence of IgM and IgA antibodies detected by ISAGA. Detection of specific IgE antibodies using ISAGA technique may be useful for differential diagnosis of acute and chronic phase of T. gondii infection and also in some cases of serological reactivation of congenital toxoplasmosis.
Źródło:
Annals of Parasitology; 1997, 43, 1; 39-51
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Zastosowanie metody ISAGA w wykrywaniu swoistych przeciwciał IgM, IgA, IgE w nabytej i wrodzonej toksoplazmozie
IMMUNOSORBENT AGGLUTINATION ASSAY (ISAGA) IN DETECTION OF SPECIFIC IgM, IgA, AND IgE ANTIBODIES IN ACQUIRED AND CONGENITAL TOXOPLASMOSIS
Autorzy:
Paul, M.
Powiązania:
https://bibliotekanauki.pl/articles/2148892.pdf
Data publikacji:
1997
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
toksoplazmoza
choroby pasozytnicze
przeciwciala swoiste
metody serologiczne
immunoglobulina M
parazytologia
immunoglobulina A
immunoglobulina E
metoda ISAGA
wykrywanie
Toxoplasma gondii
pasozyty czlowieka
Opis:
Immunocapture assays ISAGA PLUS IgA/IgM (bioMerieux) and IgE ISAGA were used to determine their usefulness in the diagnosis of acquired and congenital toxoplasmosis. Specific IgM, IgA and IgE antibodies were tested in 134 patients, namely pregnant women who seroconverted during gestation (n= 20), children with congenital toxoplasmosis (n= 5), patients with toxoplasmic lymphadenitis (n= 56) and immunocompetent individuals with chronic Toxoplasma gondii infection (n= 53). Altogether 172 sera were examined. Specific IgM antibodies were detected in all sera from pregnant women (100%) with recent T. gondii infection (1- 8 weeks after seroconversion), in all patients with toxoplasmic lymphadenopathy (1-3 months after onset of symptoms) and in their control examinations after 2 and 5 months (100%) and also in 35 (66%) out of 53 patients with chronic infection. In infants with congenital toxoplasmosis IgM were found only in one new-born; equivocal results were obtained in 3 children during the asymptomatic serological reactivation in the second year of life. Specific IgA antibodies were present in sera from 15 (75%) out of 20 women seroconverted during pregnancy; in 3 cases the results were equivocal. IgA antibodies were detected in sera from 30 (81.1%) out of 37 patients with toxoplasmic lymphadenitis examined once; in 19 patients examined 3 times IgA antibodies were present in all the cases in the first serological examination performed when clinical symptoms were first observed (100%), in 17 patients after 2 months (89.5%) and in 11 patients after 5 months (57.9%). IgA antibodies were also detected in 21 sera (39.6%) from patients with chronic T. gondii infection. In children with congenital toxoplasmosis IgA antibodies were found in 3 cases during serological reactivation after discontinuation of pyrimethamine-sulfadiazine therapy; in these cases equivocal results of IgM antibodies were present, and positive result of IgE antibodies in one case. Specific IgE antibodies were detected in sera from 17 (85%) out of 20 women with seroconversion and in 18 patients with lymphadenopathy (32.1%); in the last group IgE antibodies were not present in the follow-up examination after 5 months. IgE antibodies were detected only in 5 cases (9.4%) with chronic infection. IgA and IgE antibodies in ISAGA begin to appear about a week later than IgM antibodies; in sera collected between the 2nd and 3rd week after invasion the positive results were obtained in all cases (100%). Therefore, ISAGA PLUS IgA/IgM (bioMerieux) is useful for the diagnosis of recent T. gondii infection especially in women with suspected seroconversion during pregnancy. ISAGA PLUS IgA/IgM is more sensitive than any conventional method routinely used and so far is a specially eflicient technique for newborns and infants suspected for congenital infection and/or in diagnosing congenital toxoplasmosis during immunological recrudescence. This test has a limited value in toxoplasmosis with lymphadenopathy by reason of possibility of a long persistence of IgM and IgA antibodies detected by ISAGA. Detection of specific IgE antibodies using ISAGA technique may be useful for differential diagnosis of acute and chronic phase of T. gondii infection and also in some cases of serological reactivation of congenital toxoplasmosis.
Źródło:
Wiadomości Parazytologiczne; 1997, 43, 1; 39-51
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Trafność diagnostyczna oznaczeń alergenowo swoistych przeciwciał IgE w zawodowej alergii dróg oddechowych na czynniki o dużej masie cząsteczkowej
Diagnostic accuracy of specific IgE antibodies measurements in occupational airway allergy to high molecular weight agents
Autorzy:
Zgorzelska-Kowalik, Joanna
Walusiak-Skorupa, Jolanta
Nowakowska-Świrta, Ewa
Wiszniewska, Marta
Powiązania:
https://bibliotekanauki.pl/articles/2164218.pdf
Data publikacji:
2017-02-28
Wydawca:
Instytut Medycyny Pracy im. prof. dra Jerzego Nofera w Łodzi
Tematy:
diagnostyka
alergia zawodowa
alergenowo swoiste przeciwciała IgE
rolnicy
piekarze
alergia dróg oddechowych
diagnostics
occupational allergy
serum specific IgE antibodies
farmers
bakers
airway allergy
Opis:
Wstęp Test swoistej prowokacji wziewnej – metoda referencyjna w diagnostyce alergii zawodowej – u niektórych chorych nie może być przeprowadzony z powodu przeciwwskazań zdrowotnych. Ponieważ ustalenie rzeczywistej przydatności innych testów jest niezwykle ważne, podjęto badanie, którego celem było określenie trafności i przydatności diagnostycznej dostępnych komercyjnie odczynników do oznaczania alergenowo swoistych przeciwciał immunoglobuliny E (asIgE) w surowicy w diagnostyce IgE-zależnej alergii dróg oddechowych na najczęstsze alergeny pochodzenia roślinnego. Materiał i metody Badaniem objęto grupę 141 pacjentów – 110 piekarzy i 31 rolników – z podejrzeniem zawodowej alergii dróg oddechowych. U wszystkich badanych oznaczono asIgE dla alergenów zawodowych w surowicy odczynnikami firm Phadia i Allergopharma: u piekarzy dla mieszaniny mąk i α-amylazy, u rolników dla alergenów naskórka krowy, naskórka świni i mieszaniny piór. Metodę referencyjną do oceny powyższych testów diagnostycznych stanowił wykonany u wszystkich badanych test swoistej prowokacji wziewnej z alergenami z miejsca pracy. Wyniki Najwyższą czułością charakteryzowały się oznaczenia asIgE dla alergenów mąk (Phadia – 95,6% i Allergopharma – 88,3%), przy stosunkowo niskiej swoistości (Phadia – 47,8% i Allergopharma – 25%). Rozbieżności między wynikami uzyskanymi przy użyciu zestawów Phadia vs Allergopharma dotyczyły oznaczeń asIgE zarówno dla pojedynczych alergenów (k87, e4, e83), jak i mieszanin alergenów (fx901, fx20, ex71). Wnioski Oznaczanie asIgE w surowicy nie charakteryzuje się wystarczającą czułością, swoistością i wartością predykcyjną, żeby zastąpić test swoistej prowokacji wziewnej w diagnostyce zawodowej alergii dróg oddechowych. Med. Pr. 2017;68(1):31–43
Background The performance of specific inhalation challenge test (SICT) – reference method in diagnostics of occupational allergy – has some limitations due to health status of a particular patient. Therefore, it is extremely important to identify usefulness of other tests, and the evaluation of diagnostic accuracy of commercially available serum specific immunoglobulin E (sIgE) kits to the most common high molecular weight agents has been launched. Material and Methods The study group comprised 141 subjects – 110 bakers and 31 farmers – with suspicion of occupational airway allergy. All patients underwent evaluation of serum sIgE to occupational allergens with the use of Phadia and Allergopharma kits: in bakers to flour mix and α-amylase, in farmers to epithelium of cow, pig and feathers. Specific inhalation challenge test with workplace allergens performed in all subjects was a reference method for further analysis. Results Serum specific IgE to flour mix had the highest sensitivity (Phadia – 95.6%, Allergopharma – 88.3%), while its specificity was relatively low (Phadia – 47.8%, Allergopharma – 25%). There were numerous discrepancies between the results of sIgE estimation for particular single allergens (k87, e4, e83), as well as for their mixtures (fx901, fx20, ex71), performed with the kits of both companies (Phadia vs. Allergopharma). Conclusions Evaluation of serum specific IgE is characterized by inadequate sensitivity, specificity and predictive value to take the place of specific inhalation challenge test in diagnostics of occupational respiratory allergy. Med Pr 2017;68(1):31–43
Źródło:
Medycyna Pracy; 2017, 68, 1; 31-43
0465-5893
2353-1339
Pojawia się w:
Medycyna Pracy
Dostawca treści:
Biblioteka Nauki
Artykuł
    Wyświetlanie 1-13 z 13

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