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Wyszukujesz frazę "nodal explant" wg kryterium: Temat


Wyświetlanie 1-5 z 5
Tytuł:
Propagation of goldenrod (Solidago canadensis L.) from leaf and nodal explants
Autorzy:
Li, J.
Kang, Y.
Qiang, S.
Peng, G.
Powiązania:
https://bibliotekanauki.pl/articles/56457.pdf
Data publikacji:
2012
Wydawca:
Polskie Towarzystwo Botaniczne
Tematy:
propagation
goldenrod
Solidago canadensis
leaf
nodal explant
callus formation
micropropagation
adventitious shoot
tissue culture
Opis:
Goldenrod (Solidago canadensis L.) is an invasive plant species in many countries except North America but a cut-flower species worldwide. There is a need to generate and propagate goldenrod clones efficiently for research and commercial purposes. A callus induction and plantlet regeneration system was developed by studying the influence of explant type and different concentrations of plant growth regulators. The highest callus production from leaf segments was obtained on Murashige and Skoog's medium (MS medium) supplemented with 1.0 mg/L naphthalene acetic acid (NAA) and 1.0 mg/L 6-benzylaminopurine (BA). Adventitious shoots could be regenerated directly from leaf explants without an intermediate callus phase with the highest shoot induction percentage of 87.2%. The largest number of adventitious shoots per leaf explant (3.2) was obtained on MS medium supplemented with 0.4 mg/L NAA and 2.0 mg/L BA. MS medium supplemented with 0.1 mg/L NAA and 1.0 mg/L BA was the best medium for axillary shoot regeneration from nodal segments. The highest root number and longest roots occurred on half-strength MS without the addition of any growth regulator. Rooted plantlets were then transferred to a soil-based growth medium, placed in a greenhouse, and acclimatized with 100% success. All surviving plants grew normally without showing any morphological variation when compared to those grow from seed. This regeneration protocol may be used to produce certain biotypes of goldenrod suitable for genetic transformation, rapid propagation of goldenrod for commercial purposes or for screening fungi and toxins as potential biocontrol agents against this weed.
Źródło:
Acta Societatis Botanicorum Poloniae; 2012, 81, 1
0001-6977
2083-9480
Pojawia się w:
Acta Societatis Botanicorum Poloniae
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Lofty frequency and reproducible plant regeneration from mature nodal explants of ‘‘Egusi’’melon (Citrullus colocynthis L.)
Autorzy:
Thangavel, P.
Natarajan, S.
Shanmugam, V.
Sulaiman, S.M.
Ramasamy, R.
Powiązania:
https://bibliotekanauki.pl/articles/80168.pdf
Data publikacji:
2019
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
nodal explant
Citrullus colocynthis
melon
sterilization
mercuric chloride
shoot bud
benzylaminopurine
multiple shoot culture
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2019, 100, 3
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Micropropagation of Pluchea lanceolata (Oliver and Hiern.) using nodal explant
Autorzy:
Kher, M.M.
Joshi, D.
Nekkala, S.
Nataraj, M.
Raykundaliya, D.P.
Powiązania:
https://bibliotekanauki.pl/articles/2051.pdf
Data publikacji:
2014
Wydawca:
Instytut Ogrodnictwa
Tematy:
Pluchea lanceolata
micropropagation
nodal explant
thidiazuron
6-benzylaminopurine
kinetin
2-isopentenyladenine
medicinal plant
Compositae
antiinflammatory activity
Źródło:
Journal of Horticultural Research; 2014, 22, 1
2300-5009
Pojawia się w:
Journal of Horticultural Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Fast direct regeneration of plants from nodal explants of Stevia rebaudiana Bert.
Autorzy:
Doliński, R.
Kowalczyk, K.
Powiązania:
https://bibliotekanauki.pl/articles/12611010.pdf
Data publikacji:
2019
Wydawca:
Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
Tematy:
plant cultivation
Stevia rebaudiana
plant regeneration
plant use
medicinal plant
nodal explant
micropropagation
in vitro culture
auxin concentration
Opis:
In the preceding research, stevia has been typically cloned in vitro using two media, on which the shoots were formed (3–6 weeks), and on the other they were rooted (3–5 weeks). This study aimed at finding the possibility for rapid stevia propagation from large nodal explants using the MS basal medium [Murashige and Skoog 1962], with low auxin concentrations (0.5, 1 and 2 mg.dm–3). The plants were obtained as soon as after three weeks. The best results were obtained from media with various concentrations of the indole-3-acetic acid (IAA) and the highest concentration of phenylacetic acid (PAA). Plants were formed by 83.9–86.0% of explants, they had high weight (234–253 mg), two shoots measuring 2.07–2.37 cm and 5.8–8.3 roots measuring 1.00–1.24 cm. Mean plant weight was the lowest on media with indole-3-butyric acid (IBA) (185–192 mg). Both explant buds formed single shoots, but their development was typically uneven. The differences in the length and weight of shoots were the lowest on media with IAA and at lower PAA concentrations. Plants from the media with IAA and the control medium were distinguished by higher number of nodes. The percentage share of shoots in the total plant weight was the highest on media with PAA (62.1–62.7%), and the lowest at higher concentrations of α-naphthaleneacetic acid (NAA) (47.9 and 48.9%). Parts of explants immersed in media developed callus, and the highest amounts of this tissue were found in the media with NAA. 92.3% of plants survived the acclimatization. The applied procedure may be used for rapid in vitro cloning of selected stevia genotypes. The use of one medium enables reduction of seedling production costs. Moreover, cyclical cloning and extending the production scale is possible.
Źródło:
Acta Scientiarum Polonorum. Hortorum Cultus; 2019, 18, 5; 95-103
1644-0692
Pojawia się w:
Acta Scientiarum Polonorum. Hortorum Cultus
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Micropropagation of Merremia quinquefolia (l.) Hallier F. from nodal explants
Autorzy:
Kher, M.M.
Nataraj, M.
Parmar, H.D.
Buchad, H.
Powiązania:
https://bibliotekanauki.pl/articles/1890.pdf
Data publikacji:
2015
Wydawca:
Instytut Ogrodnictwa
Tematy:
Merremia quinquefolia
medicinal plant
Convolvulaceae
shoot multiplication
axillary shoot
cytokinin
6-benzylaminopurine
kinetin
thidiazuron
nodal explant
indole-3-butyric acid
in vitro propagation
Źródło:
Journal of Horticultural Research; 2015, 23, 1
2300-5009
Pojawia się w:
Journal of Horticultural Research
Dostawca treści:
Biblioteka Nauki
Artykuł
    Wyświetlanie 1-5 z 5

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