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Wyświetlanie 1-15 z 42
Tytuł:
Primary breast angiosarcoma – a case report
Autorzy:
Ostańska, Elżbieta
Aebisher, David
Kaznowska, Ewa
Bartusik-Aebisher, Dorota
Powiązania:
https://bibliotekanauki.pl/articles/2040145.pdf
Data publikacji:
2020-12-30
Wydawca:
Uniwersytet Rzeszowski. Wydawnictwo Uniwersytetu Rzeszowskiego
Tematy:
breast biopsy histopathological examination
immunohistochemistry
Opis:
Introduction. Angiosarcoma is a rare breast cancer that can be primary or secondary after surgery or after breast cancer radiotherapy. It is important that breast angiosarcoma belongs to tumors with a non-specific clinical and radiological picture. Aim. The study of the biopsies contained aggressive vasomotor hyperplasia. Description of the case. The presented case concerns the primary angiosarcoma of the right breast in a 56-year-old woman who had never had a surgical procedure before, nor radiotherapy in the area of the breast. Conclusion. Histopathological examination supported by immunohistochemistry is a reliable and indispensable diagnostic element in the diagnosis of vascular sarcoma.
Źródło:
European Journal of Clinical and Experimental Medicine; 2020, 4; 331-334
2544-2406
2544-1361
Pojawia się w:
European Journal of Clinical and Experimental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Epidemiological profile and distribution of prognostic factors in invasive breast cancer among Algerian women
Autorzy:
Elbasyouni, Amel
Saadi, Leila
Baha, AbdelKarim
Powiązania:
https://bibliotekanauki.pl/articles/2047226.pdf
Data publikacji:
2021-09-22
Wydawca:
Medical Education
Tematy:
breast cancer
epidemiology
biomarkers
immunohistochemistry
prognostic
Opis:
Although the widespread of early screening and advanced medical therapies, the breast cancer incidence rate continues to rise among Algerian women. This retrospective study investigated mammary lesions’ epidemiological profile and histopathological characteristics and evaluated primary invasive breast cancer prognostic factors. We found that the incidence of breast cancer increases in middle- aged women between 40 and 60 years. Scarff Bloom Richardson grade II predominates in invasive breast cancer samples. In this study, molecular profiling shows that 82.1% of invasive tumours are hormone receptor-positive. A significant correlation is observed between the age of the patient and the SBR grade (p = 0.001) and with the hormone receptor expression (p = 0.001). In addition, the tumour grade is significantly correlated to oestrogen and progesterone receptor expression (p = 0.000; p = 0.000, respectively). Twenty-two per cent of cases were human epidermal growth factor receptor 2-positive. The Ki-67 proliferation index is expressed in 91% of breast cancer patients and was significantly associated with Scarff Bloom Richardson grade (p = 0.030), the progesterone receptor expression (p = 0.029) and with human epidermal growth factor receptor 2-positivity (p = 0.023). Primary breast cancer with a high grade is more frequent (31%) in young women under 40 years old, presenting 17% of our population. In summary, breast cancer patients in Algeria develop an unfavourable profile. Immunohistochemistry assay has played a pivotal role in assessing breast cancer predictive biomarkers improving the tumour behaviour and response to treatment.
Źródło:
OncoReview; 2021, 11, 4; 95-101
2450-6125
Pojawia się w:
OncoReview
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The inflammatory reaction and the expression of pro- and anti-inflammatory cytokines to a novel polyester-polyurethane aortic prosthesis evaluated in dog sat 6 and 12 months post-implantation
Autorzy:
Mālniece, A.
Auzāns, A.
Powiązania:
https://bibliotekanauki.pl/articles/2087649.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
polyester
polyurethane
inflammation
immunohistochemistry
aortic prosthesis
Źródło:
Polish Journal of Veterinary Sciences; 2018, 21, 4; 655-664
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Protective effectiveness of anise against testicular ischemia and reperfusion injury: An experimental study in rats
Autorzy:
Yildizhan, E.
Akkus, M.
Ulger, B.V.
Asır, F.
Söker, S.
Gündüz, E.
Rençber, M.
Barçin, M.
Powiązania:
https://bibliotekanauki.pl/articles/16647584.pdf
Data publikacji:
2023
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
Anise
testicle
ischemia
reperfusion
immunohistochemistry
Opis:
Testicular torsion is a frequently encountered clinical condition that requires urgent treatment. The aim of this study is to investigate the efficacy of Anise (Pimpinella anisum L.) in treating the pathological condition due to ischemia and reperfusion injury by using biochemical, histopathological and immunohistochemical methods. A total of 6 groups were formed with 8 male Wistar Albino rats in each group. Group 1 (n=8): control group, Group 2 (n=8): Anise aqueous solution was given orally 5 ml/kg by gavage for 30 days. Group 3 (n=8): Ischemia and Reperfusion (I/R) group, bilateral testicles were rotated 270° and reperfused after 30 minutes of ischemia. Group 4 (n=8): I/R+ Anise group, Group 5 (n=8): Anise+ I/R group and Group 6 (n=8): Anise+ I/R+ Anise group. The results of the Anise group and the Control group were similar. However, the damage in the I/R group was considerably more severe than in any of the other study groups. While it was observed that spermatogenic cells started to regenerate in the I/R+Anise group, edema and congestion were observed in the Anise+I/R group. In the Anise+I/R+Anise group, all histological findings and biochemical parameters were similar to those of the control group. It was observed that anise had protective effects in ischemia and reperfusion injury in rat testicles.
Źródło:
Polish Journal of Veterinary Sciences; 2023, 26, 2; 249-255
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Immunohistochemical evaluation of the influence of L-arginine on biomarkers of environmental (cellular) stress in the kidneys of pregnant female rats
Autorzy:
Pedrycz, Agnieszka
Siermontowski, Piotr
Powiązania:
https://bibliotekanauki.pl/articles/989964.pdf
Data publikacji:
2017
Wydawca:
Instytut Medycyny Wsi
Tematy:
nitric oxide
cellular stress
kidneys
pregnancy
immunohistochemistry
Opis:
Introduction. Kidney damage during pregnancy constitutes a diagnostic and therapeutic challenge. However, it is not entirely known whether a kidney condition recognised before the pregnancy releases an organism’s response to pregnancy, or whether pregnancy itself worsens kidney function. Objective. The aim of the study was immunohistochemical evaluation of cells of kidneys of pregnant rats under the influence of nitric oxide (NO), with measurement of the immunoexpression of cellular stress markers (p-53, HSP 70). The dose of administered L-arginine (NO substrate) was approximated to that applied in obstetrics in gestosis prevention and treatment in pregnant women. Materials and method. 60 female rats used in experiment were divided into 6 groups: 3 experimental and 3 control. The females from experimental groups were administered L-arginine (40g/kg, per os) every other day starting from the seventh day or pregnancy. The animals were decapitated on the 10th, 20th day of pregnancy, and 10 days after the delivery. Kidneys taken from decapitated rats were evaluated using the immunohistochemical three step method. HSP 70 and p-53 proteins were detected. Conclusions. L-arginine increased the expression of p-53 protein – on the 10th day of pregnancy, which increased at the end of pregnancy; however, 10 days after delivery the level dropped below that observable during physiological pregnancy. Hormonal changes in physiological pregnancy cause an increase in expression of the p-53 (cell stress marker) in the epithelial cells of renal tubules, mainly at the end of pregnancy (20th day). 10 days after the delivery, this expression decreases. The expression of HSP-70 protein increases already on the 10th day of pregnancy and maintains a similar level throughout the pregnancy, but is reduced after the puerperium.
Źródło:
Annals of Agricultural and Environmental Medicine; 2017, 24, 1
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Significance of mucin expression in pancreatic intraepithelial neoplasia (PanIN) – precursor lesions of pancreatic ductal adenocarcinoma (PDAC)
Autorzy:
Zińczuk, J.
Zaręba, K.
Pryczynicz, A.
Kuczyńska, P.
Boroń, Z.
Ustymowicz, W.
Maciorkowska, M.
Ćwiklińska-Dworakowska, M.
Baszun, M.
Jelski, S.
Guzińska-Ustymowicz, K.
Powiązania:
https://bibliotekanauki.pl/articles/1918638.pdf
Data publikacji:
2018-06-18
Wydawca:
Uniwersytet Medyczny w Białymstoku
Tematy:
Mucin
immunohistochemistry
pancreatic intraepithelial neoplasia
PanIN
Opis:
Purpose: To evaluate chosen mucins (mucin 1, 4, 5AC) expression in pancreatic intraepithelial neoplasia, which is a precursor lesion of pancreatic ductal adenocarcinoma. Materials and methods: The study group included 70 patients operated on due to inflammation, cysts and pancreatic ductal adenocarcinoma with pancreatic intraepithelial neoplasia revealed additionally. Mucin 1, 4 and 5AC expression was assessed by immunohistochemical method using polyclonal antibodies. Results: Statistical analysis proved a positive correlation between the expression of mucin 1, 4 and 5AC proteins and the presence and staging of pancreatic intraepithelial neoplasia (p<0.001). Statistically significant correlations were determined between mucin 1, 4 and 5AC and the location of PanIN lesion in the pancreas. Positive correlations were found between mucin 5AC expression and the type of a basic disease (p=0.014). Differences in the expression of MUC 1, 4 and 5AC proteins between healthy pancreatic ducts and various stages of pancreatic intraepithelial neoplasia were statistically significant (p<0.001). Conclusions: Overexpression of mucin 1, 4 and 5AC is related to the presence of pancreatic intraepithelial neoplasia. This suggests that overproduction of mucus is a phenomenon occurring early in the process of carcinogenesis in the pancreas and has its beginning in precancerous lesions of an early stage.
Źródło:
Progress in Health Sciences; 2018, 8(1); 63-73
2083-1617
Pojawia się w:
Progress in Health Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Horners syndrome during spindle cell lipoma of theneck–a case report
Autorzy:
Kaczmarczyk, Dariusz
Kubiak, Marcin
Komoń-Kotecka, Ewa
Bartoszewicz, Robert
Bruzgielewicz, Antoni
Powiązania:
https://bibliotekanauki.pl/articles/1401930.pdf
Data publikacji:
2014
Wydawca:
Index Copernicus International
Tematy:
Spindle cell lipoma
Immunohistochemistry
Horner's syndrome
Opis:
Horner's syndrome is most often caused by a tumour compressing the sympathetictrunk. Spindle cell lipoma (SCL) is a rare tumour, corresponding to just 1.5% of the benigntumours of soft tissues. Its most common localisation is subcutaneous tissue in theupper part of the body. This paper presents a rare case of Horner's syndrome caused bya SCL of the neck.A 42-year-old male patient was admitted to the ENT Ward of the Mazovian SpecialHospital in Siedlce because of a neck tumour on the left side. Laryngological examinationrevealed only a hard tumour with a limited mobility on the left side of the neck. Oph-thalmological and neurological consultations indicated Horner's syndrome. Fine-needleaspiration biopsy gave no clear diagnosis. The patient was qualified for surgery. Thetumour was radically excised and sent for histopathological examination. Thefinal his-topathological diagnosis was: SCL. Symptoms of the Horner's syndrome disappearedwithin 3 weeks after the surgery.The discussed case should be noted because of untypical SCL localisation within thecervical perivascular space, and also because of presence of symptoms of Horner's synd-rome caused by compression of the tumour on the sympathetic trunk that disappearedfollowing the surgical treatment.
Źródło:
Polski Przegląd Otorynolaryngologiczny; 2014, 3, 4; 222-225
2084-5308
2300-7338
Pojawia się w:
Polski Przegląd Otorynolaryngologiczny
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Immunohistochemical study on the development of cholinergic and nitrergic nerve structures in the bovine esophageal groove
Autorzy:
Marszałek, M.
Serzysko, T.
Sienkiewicz, W.
Powiązania:
https://bibliotekanauki.pl/articles/16539106.pdf
Data publikacji:
2022
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
esophageal groove
acetylcholine
nitric oxide
immunohistochemistry
development
Opis:
The proper functioning of the perinatal sucking reflex in calves is essential for the prevention of milk leakage into the rumen. The complex process behind its regulation is mediated at the gut level via multiple excitatory and inhibitory neurotransmitters, of which acetylcholine and nitric oxide are of fundamental importance. The aim of our study was to depict age-related alterations in the cholinergic and nitrergic innervation of the esophageal groove (EG) using immunohistochemistry and Real-Time PCR methods. We found out that the highest number of cholinergic nerve cells was present in the second trimester fetuses. From this developmental stage onward, their amount was gradually decreasing and reached the lowest value in 4-year-old cows. The same developmental pattern was observed for nitrergic nerve structures with the highest percentage of nitrergic neurons in the third trimester fetuses. Our observations prove that both neuronal populations are crucial for a proper closure of EG in calves. Therefore, their contribution to a general neuronal activity in the ENS diminishes with age as the high motility of a gastric groove is not necessarily required in older cattle.
Źródło:
Polish Journal of Veterinary Sciences; 2022, 25, 1; 165-174
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Expression of follicle-stimulating hormone receptor (FSHR), protein kinase B-2 (AKT2) and adapter protein with PH domain, PTB domain, and leucine zipper (APPL1) in pig ovaries
Autorzy:
Ning Zhou
Ning Wang
Xiang Qin
Qian Liu
Juhua Wang
Hui Dong
Jie Zhou
Fugui Fang
Powiązania:
https://bibliotekanauki.pl/articles/2087861.pdf
Data publikacji:
2017
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
pigs
immunohistochemistry
FSHR
AKT2
APPL1
Źródło:
Polish Journal of Veterinary Sciences; 2017, 4; 661-667
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
High expression of CD133 – stem cell marker for prediction of clinically agressive type of colorectal cancer
Autorzy:
Kostovski, Ognen
Antovic, Svetozar
Trajkovski, Gjorgji
Kostovska, Irena
Jovanovic, Rubens
Jankulovski, Nikola
Powiązania:
https://bibliotekanauki.pl/articles/1391698.pdf
Data publikacji:
2020
Wydawca:
Index Copernicus International
Tematy:
CD133
colorectal cancer
immunohistochemistry
stem cells
Opis:
Background: Colorectal cancer (CRC) is one of the most common malignancies in the world. The cancer stem cell (CSC) markers are associated with aggressive cancer types and poor prognosis. The objective of the study was to evaluate the CD133 expression and to correlate it with clinicopathological features in patients with CRC. Material and Methods: Our study included ninety patients with CRC who underwent curative surgical resection from 2012 to 2017 at the University Clinic for Digestive Surgery, Skopje, North Macedonia. Tumor samples were first analyzed with standard histopathological methods and then the CD133 expression was investigated immunohistochemically. The level of expression of CD133 was classified semiquantitatively. Low positivity was defined as positive immunoreactivity in <50% of tumor glands, and high positivity was defined as positive immunoreactivity in ≥50% of tumor glands. Furthermore, clinicopathological features of patients were retrospectively reviewed. Results: High expression of CD133 was found in 47.8% of patients’ CRC samples. In 69.6% of patients with metastatic lesions in visceral organs we found high expression of CD133. We found statistically significant differences in the expression of CD133 between patients with and without visceral metastatic lesions (P = 0.0153), between patients with a different T category (P = 0.0119), N status (P = 0.0066) and grade (G) (P = 0.0115). Our results showed that the stage of disease has the greatest impact on expression of CD133 (P < 0.00001). Conclusion: High expression of CD133 is a useful marker for prediction of the clinically aggressive type of CRC and can be routinely implemented in standard pathohistological diagnostics.
Źródło:
Polish Journal of Surgery; 2020, 92, 3; 9-14
0032-373X
2299-2847
Pojawia się w:
Polish Journal of Surgery
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The comparison between the two most common histological subtypes of breast cancer - invasive ductal and invasive lobular breast carcinoma
Autorzy:
Fudalej, Marta
Sobiborowicz-Sadowska, Aleksandra
Mormul, Agata
Jopek, Sylwia
Borowiec, Agnieszka
Sikorski, Piotr
Patera, Janusz
Deptała, Andrzej
Badowska-Kozakiewicz, Anna M.
Powiązania:
https://bibliotekanauki.pl/articles/44426576.pdf
Data publikacji:
2024-04-07
Wydawca:
Medical Education
Tematy:
immunohistochemistry
invasive lobular carcinoma
invasive ductal carcinoma
Opis:
Introduction: Invasive lobular carcinoma (ILC) occurs in 5–15% of all cases of breast cancer. In most studies, it is found to be more common among older patients, form larger tumours and present with ill-defined margins, in comparison to invasive ductal carcinoma (IDC). Material and methods: Histological preparations were obtained from 651 patients suffering from breast cancer. Preparations stained with hematoxylin and eosin were used to identify tumour type and grading. Samples underwent a basic molecular profile evaluation encompassing ER (oestrogen receptor), PR (progesterone receptor) and human epidermal growth factor receptor 2 (HER2) expression. Results: 592 cases of IDC and 59 cases of ILC were detected. The median age was 60 in both groups. While there were no statistically significant differences between IDC and ILC in nodal status and tumour size for all age groups, IDC was more frequently diagnosed at higher grading (G3). G3 accounted for 32% of all IDC specimens compared to only 13% of ILC specimens. In both groups, the most prevalent combination of hormone receptors was ER+/PR+/HER2-. The differences in ER and PR expression were statistically significant; both were assessed as positive in most ILC cases and just over half of IDC. No HER2 amplification was noted in most cases in both cancer subtypes. Conclusions: In our study, IDC and ILC showed no difference with respect to patients’ median age at the diagnosis and local disease advancement defined by TNM. ILC cases were hormone-dependent and HER2-negative more frequently than IDC. Grade 3 tumours accounted for a higher proportion of IDC cases. This was in line with several other clinicopathological analyses of breast cancer. However, there are also several papers indicating ILC’s association with favourable prognostic features, not only in terms of hormone receptors and HER2 expression but also tumour size and nodal involvement. This underlines the fact that clear differences between IDC and ILC prognosis still cannot be established.
Źródło:
OncoReview; 2024, 14, 1; 9-15
2450-6125
Pojawia się w:
OncoReview
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Immunohistochemical characterization of nerve elements in porcine intrinsic laryngeal ganglia
Autorzy:
Sienkiewicz, W.
Klimczuk, M.
Gulbinowicz-Gowkielewicz, M.
Lepiarczyk, E.
Kaleczyc, J.
Powiązania:
https://bibliotekanauki.pl/articles/16539221.pdf
Data publikacji:
2022
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
larynx
intrinsic neurons
pig
immunohistochemistry
biologically active substances
Opis:
The present study investigated the chemical coding of neurons and nerve fibres in local laryngeal ganglia in pigs (n=5) using double-labelling immunohistochemistry. Virtually all the neurons were cholinergic in nature (ChAT- or VAChT-positive). Only very solitary, small nerve cells (presumably representing interneurons) stained intensely for adrenergic marker, DβH. Many neurons also contained immunoreactivity for NOS (91%), VIP (62.7%), NPY (24.7%), galanin (10%), SP (1.3%) and CGRP (5.3%). No neurons expressing somatostatin or Leu-enkephalin were observed. Nearly all the neuronal somata were densely supplied with varicose cholinergic nerve terminals, which presumably represented preganglionic axons, and some of them were also closely apposed with CGRP- and/or SP-positive varicose nerve endings, which were putative collaterals of extrinsic primary sensory fibres. In conclusion, this study has revealed that intrinsic neurons in the porcine larynx, like in many other mammalian species studied, should be classified as parasympathetic cholinergic neurons expressing biologically active substances, predominantly NOS and VIP. Furthermore, they are likely to receive inputs from not only preganglionic neurons but also primary sensory nerve cells. Finally, it appears that the information on the occurrence of the local laryngeal ganglia should be regularly included in textbooks dealing with the cranial portion of the parasympathetic nervous system in mammals.
Źródło:
Polish Journal of Veterinary Sciences; 2022, 25, 2; 325-334
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Dispersal and immunohistochemical characteristics of neurons in the stem of the porcine vagus nerve
Autorzy:
Sienkiewicz, W.
Klimczuk, M.
Majewska, M.
Kaleczyc, J.
Powiązania:
https://bibliotekanauki.pl/articles/16647386.pdf
Data publikacji:
2022
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
vagus nerve
intravagal neurons
neurotransmitter markers
immunohistochemistry
pig
Opis:
The present study investigated the distribution and chemical properties of nerve cell bodies within the trunk of the vagus nerve in juvenile female pigs (n=4) using double-labelling immunofluorescence. The neurons appeared mostly as single cells or formed streaks of cells or small ganglia. Many of the perikarya were cholinergic (VAChT-positive; VAChT+) or adrenergic (DβH+) in nature and no SP+ or CGRP+ neurons were encountered. There were no distinct left-right differences regarding the number and chemical coding of the neuronal somata, however, these characteristics significantly varied between particular nerve segments investigated. The vagosympathetic trunks, and thoracic and abdominal segments of the vagus nerve contained on average (the numerical values represent the means for both the left and right corresponding nerve segments) 142, 236, and 111 PGP 9.5-positive neurons, respectively. Proportions of cholinergic and adrenergic neurons were as follows: 0% and 100%, 54.2% and 33.2%, and 52.8% and 35.4%, respectively. Relatively many neurons in the thoracic and abdominal segments stained also for NOS (39.2% and 39.9%, respectively). It remains to be determined whether the porcine intravagal neurons represent a developmental relic, or whether they have any specific functional significance.
Źródło:
Polish Journal of Veterinary Sciences; 2022, 25, 4; 617-619
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Immunohistochemical properties of motoneurons supplying the trapezius muscle in the rat
Autorzy:
Dudek, A.
Sienkiewicz, W.
Marczak, M.
Kaleczyc, J.
Powiązania:
https://bibliotekanauki.pl/articles/30477.pdf
Data publikacji:
2011
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
immunohistochemical property
motoneuron
trapezius muscle
rat
tracing
immunohistochemistry
Opis:
Combined retrograde tracing (using fluorescent tracer Fast blue) and double-labelling immunofluorescence were used to study the distribution and immunohistochemical characteristics of neurons projecting to the trapezius muscle in mature male rats (n=9). As revealed by retrograde tracing, Fast blue-positive (FB+) neurons were located within the ambiguous nucleus and accessory nucleus of the grey matter of the spinal cord. Immunohistochemistry revealed that nearly all the neurons were cholinergic in nature [choline acetyltransferase (ChAT)-positive]. Retrogradely labelled neurons displayed also immunoreactivities to calcitonin gene-related peptide (CGRP; approximately 60% of FB+ neurons), nitric oxide synthase (NOS; 50%), substance P (SP; 35%), Leu5-Enkephalin (LEnk; 10%) and vasoactive intestinal polypeptide (VIP; 5%). The analysis of double-stained tissue sections revealed that all CGRP-, VIP- and LEnk-immunoreactive FB+ perikarya were simultaneously ChAT-positive. The vast majority of the neurons expressing SP- or NOS-immunoreactivity were also cholinergic in nature; however, solitary somata were ChAT-negative. FB+ perikarya were surrounded by numerous varicose nerve fibres (often forming basket-like structures) immunoreactive to LEnk or SP. They were also associated with some CGRP-, NOS- and neuropeptide Y-positive nerve terminals.
Źródło:
Polish Journal of Veterinary Sciences; 2011, 14, 2
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Określenie wartości diagnostycznej wybranych badań immunohistochemicznych u chorych na mięsaki macicy w aspekcie ich przydatności terapeutycznej i prognostycznej
Determination of diagnostic value of selected immunohistochemical tests in patients with uterine corpus sarcoma and their therapeutic and prognostic usefulness
Autorzy:
Jońska-Gmyrek, Joanna
Nasierowska-Guttmejer, Anna
Bidziński, Mariusz
Dańska-Bidzińska, Anna
Powiązania:
https://bibliotekanauki.pl/articles/908070.pdf
Data publikacji:
2007
Wydawca:
Medical Communications
Tematy:
corpus uteri sarcoma
immunohistochemistry
markers
treatment
diagnosis
immunohistochemia
Opis:
Uterine sarcomas represent a rare and heterogenous group of tumours, sometimes demonstrating multidirectional differentiation. Histological type of the sarcoma influences the choice of therapeutic modality. Aim of paper: Determination of diagnostic value of selected immunohistochemical studies (MIB1, caldesmon, desmin, SMA, SrMa, CKAE1/3) in the definition of histological type of tumour. Assessment of expression of the protein c-kit (CD117) MIB1 and inhibin and their prognostic role. Material and method: Study material consisted of a group of patients with uterine sarcoma, treated at the Center of Oncology in Warsaw, Poland, since 1999 thru 2006, from whom surgical specimens and paraffin blocks were obtained. Of 120 eligible patients, complete clinical data and specimens suitable for foreseen studies were available in 66 cases and this group became the subject of further analyses. Immunohistochemical (IHC) tests were performed using a panel of antibodies (SMA, SrMa, desmin, h-caldesmon, CD10, CKAE, MIB1, inhibin and CD117). Diagnostic value of IHC studies was assessed by descriptive statistical tests. Original histopathological findings were confronted with the results of validated IHC tests. Survival was assessed based on Kaplan-Meier curves and follow-up time – by an inverse Kaplan-Meier curve. Prognostic factors were analysed by the Cox method. Threshold of statistical significance has been set at p=0.05. Results: Original microscopic findings have had to be modified as to histological type of sarcoma in about 1/3 of the cases. Histological diagnosis was an independent prognostic factor, influencing both overall survival rate and time to tumour recurrence. Prognostic factors influencing recurrence-free survival were: invasion of perivascular lymph space (lymph space invasion, LSI), proliferative index (MB1) and severity of tumour cell necrosis. Conclusions: Application of a panel of suitable IHC tests contributes to a more precise determination of tumour type, assisting in an optimal choice of adjuvant treatment modality and thus in improvement of the patients’ prognosis.
Mięsaki macicy stanowią rzadko występującą, heterogenną grupę nowotworów, wykazującą niekiedy wielokierunkowe różnicowanie. Typ histopatologiczny mięsaka ma wpływ na wybór metody leczenia. Cel: Określenie przydatności diagnostycznej wybranych badań immunohistochemicznych (MIB1, kaldesmon, desmi-na, SMA, SrMa, CKAE1/3) w ocenie typu histopatologicznego. Ocena ekspresji białka c-kit (CD117), MIB1, oraz inhibiny w aspekcie przydatności prognostycznej. Materiał i metoda: Materiał badawczy stanowiła grupa chorych na mięsaki macicy leczonych w Centrum Onkologii - Instytucie im. Marii Skłodowskiej--Curie w Warszawie w latach 1999-2006, od których uzyskano preparaty i bloczki parafinowe. Spośród 120 chorych pełne dane kliniczne i preparaty nadające się do zastosowania badań uzyskano od 66 chorych, która to grupa stała się przedmiotem analizy. Wykonano badania immunohistochemiczne z użyciem panelu przeciwciał: SMA, SrMa, desmina, h-kaldesmon, CD10, CKAE, MIB1, inhibina oraz CD117. Do oceny wartości diagnostycznej badań immunohistochemicznych (IHC) zastosowano statystykę opisową. Zestawiono wynik pierwotnego badania histopatologicznego z wynikiem zweryfikowanym badaniami IHC. Do oceny przeżycia zastosowano krzywą Kaplana-Meiera, czas obserwacji mierzono odwrotną metodą Kaplana-Me-iera. Do analizy czynników predykcyjnych zastosowano metodę Coksa. Istotność statystyczną określono na poziomie p=0,05. Wyniki: W stosunku do rozpoznania pierwotnego typ histopatologiczny mięsaków został zmieniony w około 1/3 przypadków. Rozpoznanie histopatologiczne okazało się niezależnym czynnikiem rokowniczym mającym wpływ zarówno na przeżycie ogólne, jak i czas do nawrotu choroby. Czynnikami rokowniczymi w aspekcie przeżycia bez nawrotu choroby okazały się: zajęcie naczyniowej przestrzeni chłonnej (LSI), indeks proliferacyjny (MIB1), oraz stopień martwicy w komórkach guza. Wnioski: Zastosowanie panelu odpowiednich badań immunohistochemicznych ma wpływ na precyzyjne określenie charakteru guza, co niesie za sobą wybór odpowiedniej metody leczenia uzupełniającego, a tym samym, być może poprawę rokowania.
Źródło:
Ginekologia Onkologiczna; 2007, 5, 2; 75-86
1731-5379
Pojawia się w:
Ginekologia Onkologiczna
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-15 z 42

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