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    Wyświetlanie 1-8 z 8
    Tytuł:
    The photosynthetic activity of Paramecium bursaria endosymbiotic algae in varying temperature conditions
    Aktywność fotosyntetyczna endosymbiotycznych glonów Paramecium bursaria w zróżnicowanych warunkach temperatury
    Autorzy:
    Możdżeń, Katarzyna
    Zagata-Leśnicka, Patrycja
    Ślęczka, Mateusz
    Greczek-Stachura, Magdalena
    Powiązania:
    https://bibliotekanauki.pl/articles/2179192.pdf
    Data publikacji:
    2017-12-31
    Wydawca:
    Uniwersytet Pedagogiczny im. Komisji Edukacji Narodowej w Krakowie
    Tematy:
    emission fluorescence
    high temperatures
    PSII activity
    spectrofluorimetry
    Chlorella vulgaris
    wysoka temperatura
    aktywność PSII
    szczepy Chlorella vulgaris
    spektrofluorymetria
    Opis:
    The aim of this study was to investigate the effect of higher temperatures on the photosynthesis of endosymbiotic Chlorella sp. of two Paramecium bursaria Ehrenberg 1831 strains originating from regions with a warmer and colder climate (Ardmore - USA and Kamchatka - Russia, respectively). After seven days of protozoa incubation at 18°C (control), 21°C, 24°C, 27°C, 30°C and 33°C, the chlorophyll a fluorescence measurements were carried out and fluorescence spectra were measured in blue-green and red light. As a result of the studies, a significant effect of higher temperature on the photosynthesis process of P. bursaria endosymbionts was observed. Weekly incubation at 33°C was lethal for both protozoan strains in comparison to the control temperature (18°C). The blue-green fluorescence spectra were characterised by marked peaks at 450 nm and 490 nm. Within the red light range, the peak was observed at about 690 nm with a lesser arm at 730 nm. Endosymbionts from Kamchatka were more sensitive to the temperature increase than algae from areas with relatively warm climates.
    Celem niniejszej pracy było zbadanie wpływu podwyższonej temperatury na przebieg procesu fotosyntezy endosymbiontów z gatunku Chlorella sp. dwóch szczepów Paramecium bursaria Ehrenberg 1831, pocho-dzących z terenów o niskich i wysokich temperaturach powietrza (Ardmore – USA i Kamczatka – Rosja). Po 7 dniach inkubacji pierwotniaków w każdej z temperatur 18°C (kontola), 21°C, 24°C, 27°C, 30°C i 33°C przeprowadzono pomiary fuorescencji chloroflu a i wyznaczono widma emisji fuorescencji w zakresie niebiesko-zielonym i czerwonym.W wyniku przeprowadzonych badań zaobserwowano istotny wpływ podwyższonej temperatury na proces fotosyntezy endosymbiontów P. bursaria. Tygodniowa inkubacja w temperaturze 33°C była letalna dla obu szczepów pierwotniaka, w porównaniu z temperaturą kontrolną (18°C). Widma emisji fuorescencji niebiesko-zielonej charakteryzowały wyraźnymi pikami przy 450nm i 490 nm. W zakresie czerwonym pik zaobserwowano przy około 690 nm z mało wyraźnym ramieniem przy 730 nm. Endosymbionty szczepu pochodzącego z Kamczatki były bardziej wrażliwe na wzrost temperatury od glonów pochodzących z terenów o stosunkowo ciepłym klimacie
    Źródło:
    Annales Universitatis Paedagogicae Cracoviensis Studia Naturae; 2017, 2; 69-80
    2543-8832
    2545-0999
    Pojawia się w:
    Annales Universitatis Paedagogicae Cracoviensis Studia Naturae
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Predicting emission spectra of fluorescent materials from their absorbance spectra using the artificial neural network
    Autorzy:
    Shams-Nateri, A
    Piri, N
    Powiązania:
    https://bibliotekanauki.pl/articles/173176.pdf
    Data publikacji:
    2015
    Wydawca:
    Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
    Tematy:
    fluorescence
    prediction
    absorbance
    emission
    neural networks
    Opis:
    Artificial neural networks have been shown to be able to approximate any continuous nonlinear functions and have been used to build data based empirical models for nonlinear processes. This work studies primarily the performance of neural networks as a tool for predicting the emission spectra of fluorescent materials from their absorbance, and further, tends to the determination of the optimal topology of the neural network for this purpose. In order to do this, spectral data were initially analyzed by a principal component analysis technique. The first four principal components were used as input nodes of neural networks with various training algorithms – namely cascade- and feed-forward algorithms – and also, various numbers of hidden layers and nodes. The obtained results indicate that the RMS error in a testing data set decreased with increasing the number of neurons and the minimal network architecture for a data prediction problem consists of two hidden layers, respectively with 9 and 1 nodes for both neural networks. Additionally, a better performance was obtained with the cascade-forward neural network, especially in a small number of nodes. The obtained results indicate that the neural networks can be used to provide a relationship between the absorbance as an input and the emission as a target.
    Źródło:
    Optica Applicata; 2015, 45, 4; 545-557
    0078-5466
    1899-7015
    Pojawia się w:
    Optica Applicata
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Engine fuel characterisation based on excitation-emission spectra
    Autorzy:
    Baszanowska, E.
    Otremba, Z.
    Powiązania:
    https://bibliotekanauki.pl/articles/246580.pdf
    Data publikacji:
    2017
    Wydawca:
    Instytut Techniczny Wojsk Lotniczych
    Tematy:
    fuel
    diesel
    engine
    excitation-emission spectra
    fluorescence
    Opis:
    Considering that correctly functioning of diesel ship engine depends on the composition of applied fuel, it is necessary to easy characterise fuels in terms of quality. Therefore taking into account above mentioned, appropriate analyses have been undertaken and the intention of this article is to inform that characterisation of fuel used in marine transport can be described by the specific parameter based on fluorescence excitation-emission spectroscopy. Therefore, for this study fuel typically used in ship engine – Diesel fuel – is considered. For analysis, Diesel fuel dissolved in n-hexane was used and several oil samples for various oil concentration were prepared. Spectrofluorometer Aqualog Horiba was used to record the excitation-emission spectra (EEMs). EEM spectra determined for each of oil concentration were described by two specific peaks for this kind of oil. Based on registered EEMs, the specific indicator - the wavelength-independent fluorescence maximum – coded in two specific wavelengths – excitation and emission, respectively, described as Exmax/Emmax was determined for considered oil concentration. Obtained results indicate the independence from oil concentration only for one detected peak described by Exmax/Emmax = 240/332. Taking into account obtained results, we can conclude that the wavelength-independent fluorescence maximum detected for excitation wavelength at 240 nm corresponds to the emission wavelength at 332 nm due to the independence from the oil concentration, could be a good indicator to characterise this kind of oil – Diesel fuel. We can conclude that the changes in the composition of fuel could be detected in EEM spectra by the change of detected peaks position.
    Źródło:
    Journal of KONES; 2017, 24, 2; 25-30
    1231-4005
    2354-0133
    Pojawia się w:
    Journal of KONES
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Comparison of fluorometric signatures of oil residues in both marine environment and in organic diluent
    Autorzy:
    Otremba, Z.
    Baszanowska, E.
    Powiązania:
    https://bibliotekanauki.pl/articles/243211.pdf
    Data publikacji:
    2015
    Wydawca:
    Instytut Techniczny Wojsk Lotniczych
    Tematy:
    oil pollution
    seawater
    excitation-emission spectra
    fluorescence spectroscopy
    Opis:
    The significant part of petroleum substances is transferred to the marine environment after technical activities (inevitable exploitive spills, illegal discharges). However a certain part of petroleum in seawater coming from the natural sources. Therefore, to develop the safety of natural marine environment it is necessary to track not only the anthropogenic pollutants but also the natural sources of petroleum. To distinguish both source of oil the fluorescence properties of oil can be used as the tools to oil identification. The aim of the study is to describe the optical properties of crude oil after it contact with aquatic environment based on fluorescence spectroscopy. For the study, crude oil ‘Petrobaltic’ type potentially found in marine environment due to coming from natural source was applied. To dissolve oil, two different solvent – hexane and Baltic seawater form the Gdynia coast were used. To measure the excitation-emission spectra (EEMs) of oil spectrofluorometer Hitachi F-7000 FL was applied. In the paper, we discuss the changes of oil EEMs in two cases for oil dissolved in n-hexane and for oil dissolved in seawater for various oil concentration. Obtained EEMs for oil dissolved in seawater indicate that EEMs spectrum contain the main peak – describing by the wavelength – independent fluorescence maximum, which was detected for oil dissolved in n-hexane (typical for this kind of oil). However, the intensity of oil fluorescence decreases after oil contact with seawater. This approach could allow expanding the description of complex mixtures of oils as a possible tool proposed for identification the type and origin of oil found in marine environment.
    Źródło:
    Journal of KONES; 2015, 22, 3; 175-179
    1231-4005
    2354-0133
    Pojawia się w:
    Journal of KONES
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Spectrofluorimetry of chosen distillates of oil after pyrolysis of scrap tires
    Autorzy:
    Otremba, Z.
    Ambrosewicz-Walacik, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/245271.pdf
    Data publikacji:
    2018
    Wydawca:
    Instytut Techniczny Wojsk Lotniczych
    Tematy:
    fuels
    used car tires
    pyrolysis
    fluorescence
    excitation-emission spectra
    Opis:
    One of way to obtain liquid hydrocarbons can be pyrolysis of scrap tires. The hydrocarbon substances produced by this method may be an addition to traditional fuels, i.e. gasolines or fuels for diesel engines. In laboratory conditions at Department of Mechatronics of University of Warmia and Mazury there were obtained pyrolytic products and prepared three distillates related to temperatures 160, 204 and 350ºC. Analyses of shapes of fluorescence spectra were carried out in Department of Physics of Gdynia Maritime University. Spectra of fluorescence were prepared applying the spectrofluorimeter Hitachi F-7000 FL, which allows applying of excitation wavelength from 200 nm until 600 nm, whereas analysing of emitted, can be performed until 750 nm. In here reported study excitation and emission range for the most intense fluorescence are presented. There were prepared four solutions of pyrolytic products in the n-hexane: 4 ppm, 20 ppm, 100 ppm and 500 ppm. In order to show dependence of intensity fluorescence and shapes of excitation-emission spectra on type of distillate and concentration both kind of visualization – three-dimensional and as contour maps – are shown in this article. In analysed range of wavelengths, intensity of fluorescence grows with concentration of oil, whereas structure of the shape of spectra simplify when concentration increases. The presented effects associated with the fluorescence of pyrolysis products allow to predict the possibility of developing a method for determining the content of these substances in mixtures with petroleum refining products and other liquid hydrocarbons.
    Źródło:
    Journal of KONES; 2018, 25, 2; 262-268
    1231-4005
    2354-0133
    Pojawia się w:
    Journal of KONES
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Fluorescence spectra of oil after it contacts with aquatic environment
    Autorzy:
    Baszanowska, E.
    Otremba, Z.
    Toczek, H.
    Rohde, P.
    Powiązania:
    https://bibliotekanauki.pl/articles/245054.pdf
    Data publikacji:
    2013
    Wydawca:
    Instytut Techniczny Wojsk Lotniczych
    Tematy:
    oil pollution
    fluorescence spectroscopy
    excitation-emission spectra
    seawater
    environment protection
    Opis:
    The main task for natural marine environment protection is to prevent the inflow of various contaminants including oil substances and the real challenge is the ability to rapidly detect these pollutants. Moreover important is to find the source or the maker of the oil spill. In this paper, we consider fundamental physical aspects in the area of possibility of monitoring the natural marine ecosystem based on fluorescence spectroscopy. We utilize the fluorescence ability of numerous oil components – mainly polycyclic hydrocarbons. The paper concerns the spectrofluorimetric characterization of oils have been in use during exploitation of the marine fleet, i.e. lubricate oil, fuels, transformer and hydraulic oils as well as crude oils or their residues. Every kind of oil has a chance to enter the marine environment, especially in a case of ship emergency or after collision with other vessel ore shoreline structure as well as when ship enters the stranding. After discharge of oil, some of oil components are dissolved in the water, bacteria or photochemical reactions, which results in transformation of composition of oil, degrade some. Fluorescence spectrometer Perkin Elmer LS55 was applied to obtain the fluorescence spectra using different excitation wavelength in the range from 240 nm to 500 nm. We discuss the changes of the shapes of excitationemission spectra (EEMs) of various types of oil and the EEMs spectra after contact of oil with seawater as an oil-inwater emulsion, which is the most frequent form of oil in the water column. Significant changes in the shape of spectra and displacement of the peaks are observed.
    Źródło:
    Journal of KONES; 2013, 20, 3; 29-34
    1231-4005
    2354-0133
    Pojawia się w:
    Journal of KONES
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Properties of the Baltic crude oil in the oil-in-water emulsion form : excitation-emission spectra
    Autorzy:
    Baszanowska, E.
    Otremba, Z.
    Powiązania:
    https://bibliotekanauki.pl/articles/246400.pdf
    Data publikacji:
    2018
    Wydawca:
    Instytut Techniczny Wojsk Lotniczych
    Tematy:
    crude oil
    excitation-emission spectra
    fluorescence
    seawater
    ropa naftowa
    fluorescencja
    woda morska
    Opis:
    Due to the fact that marine environment contamination by oil substances are not uncommon, it is necessary to improve the ability of determine their origin. Therefore, research on the possibility of recognizing the type of oil using its individual characteristic manifested in the processes of fluorescence excitation has been undertaken. Oil pollutants present in seawater mainly coming from ship drives, tankers, pipelines or sea bottom seeps, moreover oil leaks from offshore extraction equipment also are possible. For this reason, the crude oil extracted from the Baltic Sea deposit in the Polish Maritime Areas has been chosen for tests. Fluorescence properties of water containing small amounts of oil (concentrations from 5.59 to 55.4 ppm) in the form of oil-in-water emulsion were tested. Individual samples were assigned matrices of fluorescent values for different wavelengths, while excited by monochromatic light also for different wavelengths. The obtained matrices were visualized as a contour maps and 3D charts. For considered concentrations of crude oil dispersed in water, the wavelength-independent fluorescence maximum was determined. Obtained result indicates that in the studied wavelength range, the total fluorescence intensity is proportional to the oil concentration only for the lowest oil concentrations. The analysis of the objective parameter of the difference between the shapes of spectra indicates the similarity of the spectral shape for the lowest oil concentrations. These results are the methodological suggestion, that for the purpose of identifying the type of oil dispersed in water, spectra should be determined for sequences of different dilutions, until the excitation-emission spectra shapes become independent from the oil concentration.
    Źródło:
    Journal of KONES; 2018, 25, 1; 7-14
    1231-4005
    2354-0133
    Pojawia się w:
    Journal of KONES
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Spectrofluorimetric characteristics of used lubricating oil
    Spektrofluorymetryczna charakterystyka zużytego oleju smarnego
    Autorzy:
    Baszanowska, E.
    Otremba, Z.
    Powiązania:
    https://bibliotekanauki.pl/articles/328239.pdf
    Data publikacji:
    2014
    Wydawca:
    Polska Akademia Nauk. Polskie Towarzystwo Diagnostyki Technicznej PAN
    Tematy:
    fluorescence
    excitation-emission spectra of oil
    lubricate oil
    ship engine
    fluorescencja
    zupełne widmo fluorescencji
    olej smarny
    silnik okrętowy
    Opis:
    Deterioration of lubricate oil in combustion engines enforces measurements of parameters essential for proper operation of define driving system or to certain engine malfunctions indicate. Since the fluorescence phenomenon is known for oil excited by ultraviolet light - the question arises whether fluorescence spectra evolving with the time of exploitation of oil in the engine. In this paper the introductory studies of excitation-emission spectra (EEMs) for chosen lubricate oil are described. Both fresh and used oil (after 593 h of work in a ship engine), was used to measure EEMs. As a result of comparison of EEMs for both fresh and used lubricate oils more significant transformations in fluorescence spectra of oil caused by their exploitation are indicated. Obtained results allow to predict that spectrofluorimetry method could supplement present methods of used lubricating oil testing.
    Pogarszanie się właściwości oleju smarnego w silniku spalinowym skutkuje koniecznością monitorowania jego eksploatacyjnych parametrów. Z racji wykazywania przez oleje fluorescencji w świetle ultrafioletowym - powstaje pytanie, czy widma fluorescencji ewoluują wraz z czasem przebywania oleju w obiegu smarnym silnika. W artykule opisane są wstępne analizy zmian zupełnych widm fluorescencji (EEMs) dla różnych stężeń wybranego oleju smarnego. Do pomiaru EEMs oleju użyto dwóch form oleju – świeżego i przepracowanego w silniku okrętowym w czasie 593 h. W następstwie porównania widm uzyskanych dla świeżego i przepracowanego oleju wskazano na zachodzące najbardziej znaczące zmiany we fluorescencji oleju smarnego. Uzyskane wyniki pozwalają sądzić, że spektrofluorymetria mogłaby uzupełnić obecne metody testowania przepracowanego oleju.
    Źródło:
    Diagnostyka; 2014, 15, 4; 65-71
    1641-6414
    2449-5220
    Pojawia się w:
    Diagnostyka
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-8 z 8

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