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Wyszukujesz frazę "embryogenic tissue" wg kryterium: Temat


Wyświetlanie 1-5 z 5
Tytuł:
Structure of embryogenic tissues and accumulation of storage materials in somatic embryos of Picea abies and P. omorika
Autorzy:
Hazubska-Przybyl, T
Bojarczuk, K.
Guzicka, M.
Powiązania:
https://bibliotekanauki.pl/articles/41109.pdf
Data publikacji:
2008
Wydawca:
Polska Akademia Nauk. Instytut Dendrologii PAN
Tematy:
Picea abies
Picea omorica
tissue
spruce
development
starch grain
lipid body
embryogenic tissue
storage material
somatic embryo
plant tissue
Opis:
Microscopic analysis of embryogenic tissues of Picea abies and P. omorika showed that the tissues differed in proportions of various types of proembryogenic masses, and in presence/absence of early somatic embryos, which suggests that they differed in embryogenic potential. On the basis of this study, embryogenic competence of tissues can be assessed at early stages of somatic embryogenesis, and thus the most efficient lines can be selected (one Picea abies and two P. omorika). In the course of development of somatic embryos embryogenic tissue was placed on a medium with abscisic acid (ABA), which affects an increase in osmotic pressure at the stage of maturation of somatic embryos and finally on the regeneration of cotyledonary embryos. In this stage of embryos development, storage materials were accumulated, which are used in specific metabolic processes during embryo development.
Źródło:
Dendrobiology; 2008, 60; 19-28
1641-1307
Pojawia się w:
Dendrobiology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Norway spruce (Picea abies) genetic transformation with modified Cry3A gene of Bacillus thuringiensis
Autorzy:
Bříza, Jindřich
Pavingerová, Daniela
Vlasák, Josef
Niedermeierová, Hana
Powiązania:
https://bibliotekanauki.pl/articles/1039537.pdf
Data publikacji:
2013
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
Cry3A gene modification
Picea abies
Agrobacterium tumefaciens and biolistic transformation
embryogenic tissue
somatic embryogenesis
Opis:
Modified versions of the Cry3A gene of Bacillus thuringiensis (Bt) were transferred into Norway spruce (Picea abies). Both the biolistic approach and Agrobacterium tumefaciens mediated procedure were employed for transformation of embryogenic tissue (ET) cultures. The latter method proved to be more efficient yielding 70 transgenic embryogenic tissue lines compared with 18 lines obtained by biolistics. The modified Cry3A genes were driven by a 35S promoter and the nptII screenable selection marker gene was used in all vectors. The transgenic ETs were molecularly characterized and converted into mature somatic embryos. Germinating embryos formed plantlets which were finally planted into perlite and their Cry3A gene transcription activities were demonstrated by RT-PCR.
Źródło:
Acta Biochimica Polonica; 2013, 60, 3; 395-400
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Embryogenic callus induction and differentiation in silver fir (Abies alba Mill.) tissue cultures
Autorzy:
Nawrot-Chorabik, K
Powiązania:
https://bibliotekanauki.pl/articles/41643.pdf
Data publikacji:
2008
Wydawca:
Polska Akademia Nauk. Instytut Dendrologii PAN
Tematy:
silver fir
Abies alba
tissue culture
embryogenic suspensor mass
somatic embryogenesis
callus induction
differentiation
Opis:
The research was conducted on explants of silver fir (Abies alba Mill.) deriving from several forest districts in southern Poland. The study encompassed the influence of the origin of plant material, type of explants, kind of substances used for explants sterilization, PPM and the type of medium on the ability to form embryogenic callus and to develop somatic embryos in silver fir explants. From the plant material collected in three sites, 57 clones were obtained from mature zygotic embryos; this produced an embryogenesis frequency of 6%. Embryogenic callus was obtained with a diameter of 65–70 mm depending on the material origin. The best medium for development of callus inducted on embryos isolated from mature silver fir seeds was the SH medium. Somatic embryos were formed in a globular stadium (24 pieces) on this medium. The 10% solution of NaOCl (used for 15 minutes) turned out to be the most effective substance for seed sterilization.
Źródło:
Dendrobiology; 2008, 59; 31-40
1641-1307
Pojawia się w:
Dendrobiology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Somatic embryogenesis and plant regeneration from hypocotyl and leaf explants of Brassica oleracea var. botrytis (cauliflower)
Autorzy:
Siong, P.K.
Taha, R.M.
Rahiman, F.A.
Powiązania:
https://bibliotekanauki.pl/articles/19314.pdf
Data publikacji:
2011
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
somatic embryogenesis
plant regeneration
hypocotyl
leaf explant
Brassica oleracea var.botrytis
cauliflower
embryogenic callus
callus
tissue culture
Opis:
We investigated direct and indirect formation of somatic embryogenesis in Brassica oleracea var. botrytis (cauliflower), a very important vegetable crop worldwide. Direct somatic embryogenesis, which is rather rare, was achieved in culture of 2-week-old hypocotyl explants of Brassica oleracea var. botrytis on MS medium supplemented with 1.0 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D) and 0.5; 1.0; and 1.5 mg/l kinetin. Initial induction of embryogenic callus was achieved on MS supplemented with very low concentrations of 2,4-D (0.05 mg/l and 0.1 mg/l). Indirect somatic embryogenesis from leaf sections was obtained on MS supplemented with 0.05 or 0.1 mg/l 2,4-D. We examined various stages of somatic embryos (globular, heart, torpedo, cotyledonary). More embryos per explant were produced through the indirect pathway (23–25) than through the direct pathway (14–19). The number of embryos produced was high. There is a potential for recurrent, repeated or secondary somatic embryogenesis, possibly an unlimited source for mass propagation and ideal for synthetic seed production in this species. Plant regeneration was achieved on half-strength MS medium without any hormones.
Źródło:
Acta Biologica Cracoviensia. Series Botanica; 2011, 53, 1
0001-5296
Pojawia się w:
Acta Biologica Cracoviensia. Series Botanica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Apoplastic markers of cell differentiation status during somatic embryogenesis in Arabidopsis thaliana
Autorzy:
Potocka, I.
Sala, K.
Kurczynska, E.U.
Powiązania:
https://bibliotekanauki.pl/articles/81256.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
conference
somatic embryogenesis
Arabidopsis thaliana
plant tissue
embryogenic culture
immature zygotic embryo
cotyledonary stage
meristematic cell
arabinogalactan protein
lipid transfer protein
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 3
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
    Wyświetlanie 1-5 z 5

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