Temat: desaturase - Katalog OPAC zbiorów

Skocz do pozycji: 1.

Tytuł:: A novel Δ12-fatty acid desaturase gene from methylotrophic yeast Pichia pastoris GS115
Autorzy:: Wei, D
Li, M
Zhang, X
Zhou, H
Xing, L
Powiązania:: https://bibliotekanauki.pl/articles/1041169.pdf
Data publikacji:: 2006
Wydawca:: Polskie Towarzystwo Biochemiczne
Tematy:: fatty acid desaturase gene
Pichia pastoris
Saccharomyces cerevisiae
linoleic acid
Opis:: The methylotrophic yeast Pichia pastoris GS115, a widely used strain in production of various heterologous proteins, especially membrane-bound enzymes, can also produce linoleic and linolenic acids, which indicates the existence of membrane-bound Δ12 and Δ15-fatty acid desaturases. This paper describes the cloning and functional characterization of a novel Δ12-fatty acid desaturase gene from this methylotrophic yeast. The open reading frame of the gene (named Pp-FAD12) is 1263 bp in size and encodes a 420-amino-acid peptide. The deduced Pp-FAD12 protein shows high identity (50-67%) with Δ12-fatty acid desaturases from other fungi. It also shows a high identity (57%) with Δ15-fatty acid desaturase (named Sk-FAD15) from Saccharomyces kluyveri. Expression of Pp-FAD12 in polyunsaturated fatty acids non-producing yeast Saccharomyces cerevisiae demonstrated that its product converted oleic acid (18 : 1) to linoleic acid (18 : 2). This result suggests that Pp-FAD12 encodes a novel Δ12-fatty acid desaturase in P. pastoris GS115. This is the first report about the cloning and functional characterization of Δ12-fatty acid desaturase gene in methylotrophic yeast.
Źródło:: Acta Biochimica Polonica; 2006, 53, 4; 753-759
0001-527X
Pojawia się w:: Acta Biochimica Polonica
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 2.

Tytuł:: Yellow lupine gene encoding stearoyl-ACP desaturase - organization, expression and potential application.
Autorzy:: Zaborowska, Żaneta
Starzycki, Michał
Femiak, Iwona
Świderski, Michał
Legocki, Andrzej
Powiązania:: https://bibliotekanauki.pl/articles/1043803.pdf
Data publikacji:: 2002
Wydawca:: Polskie Towarzystwo Biochemiczne
Tematy:: nitrogen fixation
root nodules
stearoyl-ACP desaturase
fatty acids
yellow lupine
Opis:: A gene for the Δturase specific to stearoyl-ACP (acyl carrier protein) was identified from yellow lupine (Lupinus luteus) cDNA and genomic libraries through the differential display method. The desaturase transcript appears in plants infected with Bradyrhizobium sp. (Lupinus) as revealed by Northern hybridization, RT-PCR and expression of β-glucuronidase under the desaturase promoter. A small amount of desaturase transcript was also detected in uninfected plants, which suggests that the gene does not belong to the strict nodule-specific sequences. The desaturase provides unsaturated fatty acids for additional cell membrane synthesis. During nodule and symbiosome development a peribacteroid membrane is formed and the requirement for membrane surface increases, thus the level of desaturase expression is also higher. Transgenic plants of Nicotiana tabacum with overexpression of the full-length lupine stearoyl-ACP desaturase sequence were obtained. They revealed higher content of unsaturated fatty acids (especially oleic acid) in comparison with control plants.
Źródło:: Acta Biochimica Polonica; 2002, 49, 1; 29-42
0001-527X
Pojawia się w:: Acta Biochimica Polonica
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 3.

Tytuł:: Deficiency of long-chain polyunsaturated fatty acids in phenylketonuria: a cross-sectional study
Autorzy:: Drzymała-Czyż, Sławomira
Kałużny, Łukasz
Krzyżanowska-Jankowska, Patrycja
Walkowiak, Dariusz
Mozrzymas, Renata
Walkowiak, Jarosław
Powiązania:: https://bibliotekanauki.pl/articles/1038407.pdf
Data publikacji:: 2018
Wydawca:: Polskie Towarzystwo Biochemiczne
Tematy:: phospholipids
docosahexaenoic acid
dietary intake
inborn error of metabolism
metabolic diseases
desaturase
Opis:: The etiology of altered blood fatty acid (FA) profile in phenylketonuria (PKU) is understood only partially. We aimed to determine whether FAs deficiency is dependent on the diet or metabolic disturbances. The study comprised 40 PKU patients (20 female, 20 male; aged 11 to 35 years; 12 children and 28 adults) and 40 healthy subjects (HS; 20 female, 20 male, aged 18 to 33 years). We assessed the profile of FAs (gas chromatography/mass spectrometry) and analyzed the 72-hour dietary recalls. The amount of C14:0, C16:0 and C16:1n-7, C18:1n-9 did not differ between the analyzed groups. The percentage of C18:0 was higher, while C20:3n-9, C18:2n-6, C20:2n-6, C20:4n-6, C22:4n-6, C22:5n-6 and C22:6n-3 was lower in PKU than in HS. However, C18:3n-6, C18:3n-3 and n-6/n-3 ratio were higher in PKU patients. The C20:4n-6/C20:3n-6 ratio (reaction catalyzed by Δ5-desaturase), the C22:5n-6/C22:4n-6 and the C22:6n-3/C22:5n-3 ratio (both reactions catalyzed by Δ6 desaturase) were significantly lower in PKU patients. Therefore, the deficiency of long-chain polyunsaturated fatty acids in PKU patients may result not only from inadequate supply but also from metabolic disturbances.
Źródło:: Acta Biochimica Polonica; 2018, 65, 2; 303-308
0001-527X
Pojawia się w:: Acta Biochimica Polonica
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 4.

Tytuł:: Variation in the activity of stearoyl-CoA desaturase enzyme and endogenous precursors of unsaturated fatty acids in cows milk during grazing season
Wpływ okresu wypasu pastwiskowego na poziom indeksu enzymu stearoilo-CoA desaturazy i jego endogennych prekursorów nienasyconych kwasów tłuszczowych w mleku krowim
Autorzy:: Kuczynska, B.
Puppel, K.
Nalecz-Tarwacka, T.
Czub, M.
Powiązania:: https://bibliotekanauki.pl/articles/2706.pdf
Data publikacji:: 2015
Wydawca:: Szkoła Główna Gospodarstwa Wiejskiego w Warszawie. Wydawnictwo Szkoły Głównej Gospodarstwa Wiejskiego w Warszawie
Tematy:: milk quality
nutritional quality
stearoyl-CoA desaturase
enzyme
unsaturated fatty acid
cow milk
grazing season
Opis:: Variation in the activity of stearoyl-CoA desaturase enzyme and endogenous precursors of unsaturated fatty acids in cow's milk during grazing season. The aim of this study was to determine variation in the activity of stearoyl-CoA desaturase enzyme and endogenous precursors of unsaturated fatty acids in cow's milk during the grazing season. The study was carried out in 10 certified organic farms The major criterions of choosing organic farms to the experiment were as follows: obtaining certificate for at least 5 years and abidance of the organic farming standards, possessing a herd of Black and White Polish Holstein-Friesian cows (numbering more than 30 cow. The highest concentrations of CLA cis9, trans11 isomer, TVA and LA in milk fat has been reported in June, slightly lower in July and August. While the lowest level has been recorded in May, at the beginning of grazing period. Significant correlations between MUFA and SCD (r2= 0.828 p<0.01) has been recorded. We concluded, that monitoring of SCD activity can be used as a tool to achieve high unsaturation of milk fat, during grazing period.
Wpływ okresu wypasu pastwiskowego na poziom indeksu enzymu stearoilo-CoA desaturazy i jego endogennych prekursorów nienasyconych kwasów tłuszczowych w mleku krowim. Celem pracy było określenie zmienności w aktywności enzymu 9 desaturazy SCD i koncentracji endogennych prekursorów nienasyconych kwasów tłuszczowych w mleku krów w okresie wypasu pastwiskowego. Badania przeprowadzono w 10 certyfikowanych gospodarstwach ekologicznych. Główne kryteria wyboru gospodarstw ekologicznych do eksperymentu to: posiadanie certyfikatu od co najmniej 5 lat i przestrzeganie standardów z ekologicznej produkcji, utrzymywanie minimum 30 krów rasy polskiej holsztyńsko-fryzyjskiej w stadach. Najwyższe koncentracje: izomeru CLA c9, t11, TVA i LA w tłuszczu mlekowym wykazano w czerwcu, nieco niższe w lipcu i sierpniu. Natomiast najniższe poziomy zaobserwowano w maju, w początkowym okresie wypasu pastwiskowego. Wykazano istotną korelację (p≤0,01) między MUFA i SCD (r2=0,828). Podsumowując, monitorowanie aktywności SCD może być użyteczne jako narzędzie w celu zwiększania nienasycenia tłuszczu mleka pozyskiwanego w trakcie wypasu pastwiskowego.
Źródło:: Annals of Warsaw University of Life Sciences - SGGW. Animal Science; 2015, 54[1]
1898-8830
Pojawia się w:: Annals of Warsaw University of Life Sciences - SGGW. Animal Science
Dostawca treści:: Biblioteka Nauki