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    Wyświetlanie 1-9 z 9
    Tytuł:
    Effect of zinc oxide nanoparticles on the expression of proinflammatory proteins in murine macrophages raw 264.7 cells
    Autorzy:
    OLBERT, Magdalena
    GRYC, Karolina
    SROCZYŃSKA, Katarzyna
    ZAJĄC, Anna
    LIPKOWSKA, Anna
    LIBROWSKI, Tadeusz
    GDULA-ARGASIŃSKA, Joanna
    Powiązania:
    https://bibliotekanauki.pl/articles/1033697.pdf
    Data publikacji:
    2017
    Wydawca:
    Zakład Opieki Zdrowotnej Ośrodek Umea Shinoda-Kuracejo
    Tematy:
    COX-2
    Nrf2
    inflammation
    zinc oxide nanoparticles
    Opis:
    Zinc is a microelement essential for the body. It has a great impact on the proper development and renewal of tissues, reproductive system, skin condition, or immune processes. Zincis involved practically in all aspects of the immune system and the production and activation of white blood cells. This work aimed to determine the effect of zinc oxide nanoparticles (ZnONP) on the expression of pro-inflammatory proteins in murine macrophages RAW 264.7, activated with lipopolysaccharide (LPS). Using the immunodetection technique the expression of cyclooxygenase 2 (COX-2), prostaglandin E2 synthase (cPGES), prostaglandin F2α receptor (FP receptor) and nuclear factor Nrf2 was determined. Statistically the highest expression of COX-2, cPGES, and FP receptor was observed in LPS-activated macrophages. RAW 264.7 cells supplementation with ZnONP 100 nmol and 500 nmol and LPS activation resulted in repression of COX-2 and cPGES, and an increased expression of Nrf2 protein when compared to control. The results suggest an anti-inflammatory effect and activation of the antioxidant system by ZnONP in RAW 264.7 macrophages. It seems appropriate to conduct further research on the molecular mechanism of action of ZnONP in eukaryotic cells.
    Źródło:
    Medicina Internacia Revuo; 2017, 28, 109; 262-265
    0465-5435
    Pojawia się w:
    Medicina Internacia Revuo
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Novel Aminoketooxime Ligand and Its Cu(II) and Mn(II) Complexes: Synthesis, Characterization and Molecular Docking Studies
    Autorzy:
    Gorgulu, G.
    Cicek, M.
    Dede, B.
    Powiązania:
    https://bibliotekanauki.pl/articles/1029789.pdf
    Data publikacji:
    2018-02
    Wydawca:
    Polska Akademia Nauk. Instytut Fizyki PAN
    Tematy:
    oxime
    dinuclear complex
    antiferromagnetic
    docking
    VEGFR-2
    COX-2
    Opis:
    A novel ligand, N,N"-(4-methyl-1,2-phenylene)bis(2-(biphenyl-4-yl)-N'-hydroxy-2-oxoacetimidamide) (H₂L) with its Cu(II) and Mn(II) complexes were synthesized in this study. All compounds synthesized were also characterized by ¹H- and ¹³C-NMR, the Fourier transform infrared, elemental analysis, inductively coupled plasma optical emission spectrometry, molar conductivity, magnetic susceptibility measurements and thermogravimetric analysis. Vascular endothelial growth factor-2 (VEGFR-2) and cyclooxygenase-2 (COX-2) inhibition is often used as a parameter for being a potent anticancer agent in docking studies. For this purpose, synthesized and characterized ligand was investigated by molecular docking study to test its inhibitory effect against angiogenic factors VEGFR-2 and COX-2.
    Źródło:
    Acta Physica Polonica A; 2018, 133, 2; 250-255
    0587-4246
    1898-794X
    Pojawia się w:
    Acta Physica Polonica A
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    A paradigm shift in the role of NSAIDs in COVID-19: new pathological mechanisms and potential treatment targets
    Autorzy:
    Ilie, Petre Cristian
    Stefanescu, Simina
    Veronese, Nicola
    Powiązania:
    https://bibliotekanauki.pl/articles/1491092.pdf
    Data publikacji:
    2020-09-20
    Wydawca:
    Atena Research Association
    Tematy:
    Paradigm Shift
    NSAIDs
    Covid-19
    COX-2 inhibitors
    Opis:
    Objectives: The pathogeny of SARS-COV2 infection is currently not well defined. In this paper, we present a new perspective of how the SARS-CoV2 infection can lead to severe cases and potential pathways of preventing such cases. Methods: In the first part, we describe the role of cyclooxigenase 2 and in the second part, we describe the role of hypoxia inducible factor (HIF). Findings: We hypothesize that cyclooxigenase 2 and hypoxia inducible factor with subsequent inflammation and hypoxia can up-regulate each other in a vicious circle of feed-forward that can ultimately lead to “cytokine storm”. Conclusions: Breaking this cycle early potentially will have beneficial effects either by improving oxygenation (oxygen being given earlier in the course of the treatment) or by inhibiting COX-2. We also believe, that the use of COX-2 inhibitors early in the course of the disease can improve the outcome and clinical trial are urgently needed. 
    Źródło:
    Atena Journal of Public Health; 2020, 2; 6-6
    2695-5911
    Pojawia się w:
    Atena Journal of Public Health
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Effect of high fat diet on structure of liver and gallbladder of adult male mice – an experimental study
    Autorzy:
    Hegazy, Abdelmonem Awad
    Qenawy, Noura M.
    Aziz, Nada M. Abdel
    El-Bestawy, Emtethal M.
    Powiązania:
    https://bibliotekanauki.pl/articles/2040247.pdf
    Data publikacji:
    2021-12-30
    Wydawca:
    Uniwersytet Rzeszowski. Wydawnictwo Uniwersytetu Rzeszowskiego
    Tematy:
    COX-2
    fatty liver
    gallbladder epithelium
    immunoexpression
    Oil Red O staining
    Opis:
    Introduction. High fat diet (HFD) intake induces obesity and adversely affects different body organs including liver and gallbladder. Aim. It was to clarify the effects of HFD on the liver and gallbladder structure using light microscopic (LM) examination. Material and methods. 16 healthy adult male mice were equally divided into 2 groups. Control group mice were fed normal diet. HFD group was fed using HFD. At the end of the 8-week experiment, mice were anesthetized. Liver and gallbladder were removed and prepared to histological processing. Sections were stained with hematoxylin and eosin (H&E) and immunostaining for cyclooxygenase-2 (COX-2) cellular localization. Oil Red O (ORO)-stained frozen liver sections were prepared. Results. H&E-stained sections of HFD group revealed rounded swollen hepatic cells with pale cytoplasm suggesting cellular ballooning. Dilated congested sinusoids and portal vein, cellular degeneration and collection of inflammatory cells were observed between hepatic cells and in portal region. Gallbladder sections showed epithelial stratification and cellular vacuolation. Strong immunoexpression of COX-2 was observed in Kupffer and hepatic cells of the liver and gallbladder mucosal epithelial cells. Conclusion. HFD is suggested to alter the normal histological features of liver and gallbladder represented by fatty liver and gallbladder epithelial hyperplasia and inflammatory reaction.
    Źródło:
    European Journal of Clinical and Experimental Medicine; 2021, 4; 291-298
    2544-2406
    2544-1361
    Pojawia się w:
    European Journal of Clinical and Experimental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The influence of selective COX-2 inhibitor on phase of healing surgical wounds: proliferation and secretion of bFGF by endothelial cells
    Autorzy:
    Jasiak, Łukasz
    Kowalczyk, Mateusz
    Mazan, Paula
    Kowalczyk, Edward
    Sienkiewicz, Monika
    Jóźwiak-Bębnista, Marta
    Wiktorowska-Owczarek, Anna
    Powiązania:
    https://bibliotekanauki.pl/articles/763865.pdf
    Data publikacji:
    2017
    Wydawca:
    Uniwersytet Marii Curie-Skłodowskiej. Wydawnictwo Uniwersytetu Marii Curie-Skłodowskiej
    Tematy:
    angiogenesis, selective COX-2 inhibitor, fibroblast growth factor, vascular endothelial cell
    Opis:
    The process of wound healing consists of the following phases: inflammation, proliferation, remodeling. Non-steroidal antiinflammatory drugs may be important in this process, especially in a stage called angiogenesis. For this reason, it was decided to investigate the effect of selective COX-2 (cyclooxygenase 2) inhibitor (NS-398) on the proliferation of endothelial cells and their ability to secrete bFGF (fibroblast growth factor) for vascular endothelial cells (HMEC-1). For determination of the secretion of bFGF in a cell line HMEC-1 immunosorbent ELISA assays were used. In turn, the cell proliferation assay was performed using the MTT method. Using MTT method, it was found that NS-398 at 10 μM did not affect cell viability. Whereas selective COX-2 inhibitor at 100 μM decreased cell viability in a statistically significant manner and inhibited the proliferative effect of 100 μg/mL LPS at concentrations of 10 and 100 μM. In the further step, application of NS-398 (10 and 100 μM) with LPS (100 μg/mL; inflammatory environment) reduced the secretion of bFGF in a statistically significant manner. The investigations showed that NS-398 has an antiangiogenic effect which is based on reducing the proliferation of vascular endothelial cells and inhibiting the secretion of bFGF- factor responsible for angiogenesis during wound healing.
    Źródło:
    Annales Universitatis Mariae Curie-Sklodowska, sectio C – Biologia; 2017, 72, 1
    2083-3563
    0066-2232
    Pojawia się w:
    Annales Universitatis Mariae Curie-Sklodowska, sectio C – Biologia
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Cyklooksygenaza-2 i jej rola w kancerogenezie
    Autorzy:
    Sitarz, Robert
    Kolasińska-Bzoma, Małgorzata
    Skórzewska, Magdalena
    Polkowski, Wojciech P.
    Maciejewski, Ryszard
    Powiązania:
    https://bibliotekanauki.pl/articles/1034192.pdf
    Data publikacji:
    2012
    Wydawca:
    Uniwersytet Jagielloński. Wydawnictwo Uniwersytetu Jagiellońskiego
    Tematy:
    cyklooksygenaza
    enzym cyklooksygenazy
    neoangiogeneza
    rak
    reakcje katalizowane przez COX-2 oraz COX-1
    syntazą prostaglandyny H
    terapia chorób nowotworowych
    Opis:
    Cyclooxygenase is an enzyme involved in many physiological and pathological processes. It catalyzes the formation of prostaglandins, prostacyclins and thromboxanes from arachidonic acid. It has been reported that cyclooxygenase-2 (COX-2) modulates the secretion of proinflammatory mediators and its expression is associated with the promotion of the process of tumorigenesis initiation, transformation, progression and cancer metastasis. Increased expression of COX-2 in premalignant lesions makes it a key element in a chain of changes leading to cancer. So far, advances in the evaluation of COX -2 molecular mechanisms did not bring spectacular discoveries. Pathomechanisms of COX-2 activities are still not sufficiently understood and therefore, require further research. Currently, COX-2 inhibitors are one of the most commonly used medications. Unfortunately, their long-term use causes many side effects. The first report about the use of plant substances with anti-inflammatory and analgesic activity (COX-2 inhibitors) was reported by Hippocrates in the 5th century BC. The present review discusses the most important mechanisms by which a particular COX-2 isomer leads to the formation and development of cancer.
    Źródło:
    Zeszyty Naukowe Towarzystwa Doktorantów Uniwersytetu Jagiellońskiego. Nauki Ścisłe; 2012, 5; 19-39
    2082-3827
    2084-977X
    Pojawia się w:
    Zeszyty Naukowe Towarzystwa Doktorantów Uniwersytetu Jagiellońskiego. Nauki Ścisłe
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Influence of αlpha-linolenic acid supplementation on murine macrophages RA W 264.7 activated with lipopolisaccharide
    Autorzy:
    ZAJĄC, Anna
    SROCZYŃSKA, Katarzyna
    LIPKOWSKA, Anna
    OLBERT, Magdalena
    LIBROWSKI, Tadeusz
    SAŁAT, Kinga
    MALIKOWSKA, Natalia
    GDULA-ARGASIŃSKA, Joanna
    Powiązania:
    https://bibliotekanauki.pl/articles/1033804.pdf
    Data publikacji:
    2017
    Wydawca:
    Zakład Opieki Zdrowotnej Ośrodek Umea Shinoda-Kuracejo
    Tematy:
    COX-2
    FP receptor
    cPGES
    lipopolysaccharide
    macrophage RAW 264.7
    α – linolenic acid
    Opis:
    Chronic inflammation is characterized by excessive production of cytokines and eicosanoids and is associated with unsufficient resolution. Supplementation with n-3 fatty acids may result in a lower incidence of many inflammatory diseases. The aim of this study was to determine the effect of α – linolenic acid (ALA) on the fatty acids profile of cell membranes and on the pro-inflammatory proteins cyclooxygenase – 2 (COX-2), prostaglandin E2 synthase (cPGES) and prostaglandin F2α receptor (FP) expression in murine RAW 264.7 macrophages, activated with lipopolysaccharide (LPS). It has been shown that COX-2, cPGES as well as FP receptor expression was highest in cells activated by LPS. In macrophages supplemented with ALA and activated with LPS a pro-inflammatory protein levels were significantly reduced, suggesting anti-inflammatory activity of α-linolenic acid. There were also statistically significant changes in the fatty acid profile after incubation of the RAW 264.7 cells for 48 hours with ALA. A deficiency or excess of specific fatty acids affect the cellular membrane fluidity, can also cause changes in cell morphology. Therefore it is appropriate to carry out further research on the ALA properties.
    Źródło:
    Medicina Internacia Revuo; 2017, 28, 108; 176-181
    0465-5435
    Pojawia się w:
    Medicina Internacia Revuo
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Flavanols from Japanese quince (Chaenomeles japonica) fruit suppress expression of cyclooxygenase-2, metalloproteinase-9, and nuclear factor-kappaB in human colon cancer cells
    Autorzy:
    Owczarek, Katarzyna
    Hrabec, Elżbieta
    Fichna, Jakub
    Sosnowska, Dorota
    Koziołkiewicz, Maria
    Szymański, Jacek
    Lewandowska, Urszula
    Powiązania:
    https://bibliotekanauki.pl/articles/1038623.pdf
    Data publikacji:
    2017
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    flavanols
    Japanese quince (Chaenomeles japonica)
    colon cells
    COX-2
    MMP-9
    NF-κB
    Opis:
    Natural polyphenols and polyphenol-rich extracts have been found to possess preventive and therapeutic potential against several types of cancers, including colorectal cancer (CRC), which is an example of an inflammation-associated cancer. This study examines the chemopreventive effect of a Japanese quince (Chaenomeles japonica) fruit flavanol preparation (JQFFP) on colon cancer SW-480 cells. JQFFP, rich in procyanidin monomers and oligomers, was found to inhibit the SW-480 cell viability by 40% at 150 µM catechin equivalents (CE) after 72 h incubation when compared to control, but it was non-toxic to normal colon fibroblast CCD-18Co cells. Furthermore, 100 µM CE JQFFP suppressed COX-2 mRNA expression to 36.7% of control values and protein expression to 77%. In addition, JQFFP reduced the MMP-9 protein expression (to 24% vs. control at 100 µM CE) and caused inhibition of its enzymatic activity (to 35% vs. control at 100 µM CE). Not only did JQFFP inhibit the COX-2 and MMP-9 levels, but it also reduced the NF-κB protein expression (to 65% of control) and phosphorylation of its p65 subunit (to 51%) at 100 µM CE. These results provide the first evidence that JQFFP inhibits COX-2, MMP-9, and NF-κB expression, suggesting that it has cytotoxic, anti-inflammatory, and anti-metastatic activities towards the colon cancer SW-480 cells.
    Źródło:
    Acta Biochimica Polonica; 2017, 64, 3; 567-576
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Czy poziom ekspresji COX-2 oraz iNOS może być czynnikiem prognostycznym w diagnozie mięśniaków macicy?
    Can the level of expression of COX be a prognostic factor in the diagnosis uterine leiomymatosis?
    Autorzy:
    Morek, Michał
    Bogunia, Edyta
    Plewka, Andrzej
    Miśkiewicz, Adam
    Jakubiec-Bartnik, Beata
    Powiązania:
    https://bibliotekanauki.pl/articles/1038587.pdf
    Data publikacji:
    2012
    Wydawca:
    Śląski Uniwersytet Medyczny w Katowicach
    Tematy:
    cox-2
    inos
    macica
    mięśniak macicy
    wiek reprodukcyjny
    wiek okołomenopauzalny
    uterus
    uterine myomas
    reproductive age
    perimenopausal age
    Opis:
    INTRODUCTION Fibroids are the most common tumors of benign nature of the female reproductive organ. Before the age of 40 this problem aff ects nearly 30% of female Caucasian population. Diagnosis of fi broids is based on a gynecological examination and ultrasound. Additionally the diagnosis also applies histerosonography, CT scan and MRI scan. COX-2 is induced isoform synthesized in response to the physical, chemical or biological stimulation. Is expressed, inter alia, in tumor tissues. Stimulation of iNOS and NO separation occurring in the pathological changes play an important role in the development of cancer. AIM OF STUDY Therefore it seems to be justifi ed to determine the expression of COX-2 and iNOS in the benign tumor cells of uterine fi broids. For exact analysis of the expression of these factors also decided to get outskirts fragments of fi broids and shows a comparison of observed expressions with the results obtained with fi broids. MATERIAL AND METHODS Pure control comprised 20 patients in whom there were no fi broidal changes, both in the reproductive age and perimenopausal (10 patients each). To the research qualifi ed 20 women in childbearing age and 20 women in perimenopausal age all with fi broidal changes in uterus. In the studied biopsied tissue evaluated the optical density of cells with the expression of selected proteins like cyclooxygenase-2 (COX-2) and induced nitric oxide synthase (iNOS). RESULTS AND CONCLUSIONS Expression of COX-2 and iNOS was higher in the myoma and in the surrounding myometrium fi broids compared with the expression of these proteins in healthy women uterus myometrium. Increased expression of COX-2 and iNOS at the periphery of uterine fi broids can decide the appearance of new tumors. Therefore, you should consider the use of the expression levels of COX-2 and iNOS as a prognostic factor.
    WSTĘP Mięśniaki macicy są najczęstszymi łagodnymi nowotworami żeńskiego narządu rodnego. Przed 40 rokiem życia problem ten dotyczy blisko 30% kobiet populacji kaukaskiej. Diagnostyka mięśniaków opiera się na badaniach ginekologicznym oraz ultrasonograficznym. Pomocniczo stosuje się również histerosonografi ę, tomografi ę komputerową oraz rezonans magnetyczny. Cyklooksygenaza 2 (COX-2) jest izoformą indukowalną, syntetyzowaną w odpowiedzi na stymulację fizyczną, chemiczną lub biologiczną. Ulega ekspresji m.in. w tkankach nowotworowych. Stymulacja indukowalnej syntazy tlenku azotu (iNOS) oraz wydzielanie NO zachodzące w zmianach patologicznych, odgrywają istotną rolę w rozwoju nowotworu. CEL PRACY Uzasadnione zatem wydaje się określenie ekspresji COX-2 i iNOS w niezłośliwych komórkach nowotworowych mięśniaków macicy. W celu dokładnej analizy ekspresji tych czynników zdecydowano się także na pobranie fragmentów obrzeża mięśniaków i porównanie zaobserwowanej ekspresji z wynikami otrzymanymi z mięśniaków. MATERIAŁ I METODY Kontrolę czystą stanowiło 20 pacjentek, u których nie stwierdzono zmian mięśniakowatych, zarówno w wieku reprodukcyjnym, jak i okołomenopauzalnym (po 10 pacjentek). Do badań zakwalifikowano po 20 kobiet w wieku rozrodczym ze zmianami mięśniakowymi w macicy oraz z mięśniakami w wieku okołomenopauzalnym. W badanych wycinkach tkankowych dokonano oceny gęstości optycznej komórek z ekspresją wybranych enzymów, tj. COX-2 oraz iNOS. WYNIKI I WNIOSKI Ekspresja COX-2 oraz iNOS była wyższa zarówno w mięśniaku, jak i w myometrium otaczającym mięśniak w porównaniu z ekspresją tych białek w myometrium macicy kobiet zdrowych. Zwiększona ekspresja COX-2 i iNOS na obrzeżu mięśniaków macicy może decydować o pojawieniu się nowych guzów. Dlatego też należy rozważyć wykorzystanie poziomu ekspresji COX-2 i iNOS jako czynników prognostycznych.
    Źródło:
    Annales Academiae Medicae Silesiensis; 2012, 66, 1; 28-34
    1734-025X
    Pojawia się w:
    Annales Academiae Medicae Silesiensis
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-9 z 9

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