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Wyszukujesz frazę "Lityńska, Anna" wg kryterium: Autor

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Wyświetlanie 1-15 z 17
Tytuł:
Purification of rat liver arylsufatase A and its microheterogeneity assayed by crossed affinity-immunoelectrophoresis
Autorzy:
Wójczyk, Bogusław
Hoja, Dorota
Lityńska, Anna
Powiązania:
https://bibliotekanauki.pl/articles/1045566.pdf
Data publikacji:
1992
Wydawca:
Polskie Towarzystwo Biochemiczne
Źródło:
Acta Biochimica Polonica; 1992, 39, 4; 355-367
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Adhesion properties of human bladder cell lines with extracellular matrix components: the role of integrins and glycosylation.
Autorzy:
Lityńska, Anna
Przybyło, Małgorzata
Pocheć, Ewa
Laidler, Piotr
Powiązania:
https://bibliotekanauki.pl/articles/1043727.pdf
Data publikacji:
2002
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
glycosylation
integrins
bladder cell lines
adhesion
Opis:
Integrin subunits present on human bladder cells displayed heterogeneous functional specificity in adhesion to extracellular matrix proteins (ECM). The non-malignant cell line (HCV29) showed significantly higher adhesion efficiency to collagen IV, laminin (LN) and fibronectin (FN) than cancer (T24, Hu456) and v-raf transfected (BC3726) cell lines. Specific antibodies to the α2, a5 and β1 integrin subunits inhibited adhesion of the non-malignant cells, indicating these integrin participation in the adhesion to ECM proteins. In contrast, adhesion of cancer cells was not inhibited by specific antibodies to the β1 integrin subunit. Antibodies to α3 integrin increased adhesion of cancer cells to collagen, LN and FN, but also of the HCV29 line with colagen. It seems that α3 subunit plays a major role in modulation of other integrin receptors especially in cancer cells. Differences in adhesion to ECM proteins between the non-malignant and cancer cell lines in response to Gal and Fuc were not evident, except for the v-raf transfected cell line which showed a distinct about 6-fold increased adhesion to LN on addition of both saccharides. N-Acetylneuraminic acid inhibited adhesion of all cell lines to LN and FN irrespective of their malignancy.
Źródło:
Acta Biochimica Polonica; 2002, 49, 3; 643-650
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Differences of α3β1 integrin glycans from different human bladder cell lines.
Autorzy:
Lityńska, Anna
Przybyło, Małgorzata
Książek, Dorota
Laidler, Piotr
Powiązania:
https://bibliotekanauki.pl/articles/1044370.pdf
Data publikacji:
2000
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
integrins
bladder cell lines
oligosaccharides
Opis:
Expression as well as properties of integrins are altered upon transformation. Cell adhesion regulated by integrins is modulated by glycosylation, one of the most frequent biochemical alteration associated with tumorogenesis. Characterisation of carbohydrate moieties of α3β1 integrin on the cultured human bladder carcinoma (T-24, Hu456, HCV 29T) and human normal ureter and bladder epithelium (HCV 29, Hu609) cell lines was carried out after an electrophoresis and blotting, followed by immunochemical identification of α3 and β1 integrin chains and analysis of their carbohydrates moieties using highly specific digoxigenin-labelled lectins. In all the studied cell lines α3β1 integrin was glycosylated although in general each subunit differently. Basic structures recognized in β1 subunit were tri- or tetraantennary complex type glycans in some cases sialylated (T-24, HCV 29, HCV 29T) and fucosylated (Hu609, HCV 29T). Positive reaction with Phaseolus vulgaris agglutinin and Datura stramonium agglutinin suggesting the presence of β1-6 branched N-linked oligosaccharides was found in cancerous cell lines (T-24, Hu456) as well as in normal bladder epithelium cells (Hu609). High mannose type glycan was found only in β1 subunit from Hu456 transitional cell cancer line. On the other hand α3 subunit was much less glycosylated except the invasive cancer cell line T-24 where high mannose as well as sialylated tri- or tetraantennary complex type glycans were detected. This observation suggests that changes in glycosylation profile attributed to invasive phenotype are rather associated with α3 not β1 subunit.
Źródło:
Acta Biochimica Polonica; 2000, 47, 2; 427-434
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Towards understanding the role of sialylation in melanoma progression
Autorzy:
Kolasińska, Ewa
Przybyło, Małgorzata
Janik, Marcelina
Lityńska, Anna
Powiązania:
https://bibliotekanauki.pl/articles/1038776.pdf
Data publikacji:
2016
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
integrins
lectins
melanoma
progression
sialic acids
sialylation
Opis:
Aberrant expression of sialic acids or altered linkage types is closely associated with malignant phenotype and metastatic potential, and can have prognostic significance in human cancer. The present study was undertaken to evaluate whether expression of sialylated derivatives on melanoma cell surface is associated with tumour progression. Four cell lines (WM1552C, WM115, IGR-39 and WM266-4) were used in the study. Cell surface expression of sialic acids was evaluated by flow cytometry with the use of Maackia amurensis and Sambucus nigra lectins. Moreover, adhesion and migration potential of melanoma cells and involvement of sialic acids in these processes were analysed. We have demonstrated that WM266-4 cells have a significantly higher level of α2,3-linked sialic acid residues than other cells, whereas IGR-39 cells had lower expression of α2,6-linked sialic acids. The adhesion efficiencies of WM1552C and WM115 cells were significantly lower than that of IGR-39 and WM266-4 cells. In contrast, WM266-4 cells repaired scratch wounds at least twice as fast as other cells. Melanoma cell adhesion to fibronectin in the presence of Sambucus nigra agglutinin (SNA) was reduced only in IGR-39 and WM266-4 cells, whereas the impact of Maackia amurensis agglutinin (MAA) on this process was much more important. Migration efficiency of melanoma cells was reduced more strongly in the presence of MAA than SNA. In conclusion, our results show that melanoma progression is associated with the increased expression of α2,3-linked sialic acids on the cell surface and these residues could promote melanoma cell interaction with fibronectin.
Źródło:
Acta Biochimica Polonica; 2016, 63, 3; 533-541
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Diverse expression of N-acetylglucosaminyltransferase V and complex-type β1,6-branched N-glycans in uveal and cutaneous melanoma cells
Autorzy:
Pocheć, Ewa
Rydlewska, Magdalena
Przybyło, Małgorzata
Lityńska, Anna
Powiązania:
https://bibliotekanauki.pl/articles/1039114.pdf
Data publikacji:
2015
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
β1,4-N-acetylglucosaminyltransferase III
β1,6-N-acetylglucosaminyltransferase V
bisected GlcNAc
β1,6-branched N-glycans
uveal melanoma
cutaneous melanoma
Opis:
Although both uveal (UM) and cutaneous (CM) melanoma cells derive from the transformed melanocytes, their biology varies significantly in several aspects. Malignant transformation is frequently associated with alternations in cell glycosylation, in particular those concerning branched complex-type N-glycans. These changes occur principally in β1,4-N-acetylglucosaminyltransferase III (GnT-III) that catalyzes the synthesis of glycans with bisected N-acetylglucosamine (GlcNAc) and β1,6-N-acetylglucosaminyltransferase V (GnT-V) that is involved in forming β1,6-branched antenna in complex-type glycans. We searched for the reasons of a different behavior of CM and UM cells in the expression of GnT-III and GnT-V and their oligosaccharide products. Our study showed that UM cells have more β1,6-branched glycans than CM cells, what results from a higher expression of MGAT5 gene encoding GnT-V. The higher β1,6-branching of glycans in UM may contribute to their higher potential to migrate on fibronectin and weaker binding to main extracellular matrix proteins, observed in our previous studies.
Źródło:
Acta Biochimica Polonica; 2015, 62, 2; 323-328
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of α3β1 and αvβ3 integrin glycosylation on interaction of melanoma cells with vitronectin
Autorzy:
Janik, Marcelina
Przybyło, Małgorzata
Pocheć, Ewa
Pokrywka, Małgorzata
Lityńska, Anna
Powiązania:
https://bibliotekanauki.pl/articles/1040422.pdf
Data publikacji:
2010
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
adhesion
integrin
glycosylation
migration
vitronectin
Opis:
The metastatic transformation of melanocytes is associated with altered expression of adhesion molecules, including αvβ3 and α3β1 integrins. Integrin αvβ3 is a primary vitronectin (VN) receptor, while both integrin types take part in adhesion to VN when they are in complex with uPAR. Although their role in melanoma cell interaction with VN is of great interest, the influence of N-oligosaccharides attached to these glycoproteins is still unappreciated. The present study assesses the role of αvβ3 and α3β1 integrins and the influence of their glycosylation status on WM9 and WM239 metastatic melanoma cell interactions with VN. Cell adhesion to and migration on VN were selected as the studied cell behaviour parameters. Functionblocking antibodies and swainsonine (SW) treatment were used in these tests. Both cell lines interacted with VN in an integrin-mediated but cell-line-specific manner. In WM9 cells, migration was not completely inhibited by antibodies against α3β1 or αvβ3 integrins, suggesting the participation of other VN receptors. In both cell lines in coprecipitation test the formation of an integrins/uPAR complex was shown. In the presence of SW formation of the complex did not occur, suggesting the participation of glycosylation in this proccess. Additionally, the adhesion properties of WM9 cells were changed after SW treatment. Our results suggest that in these two metastatic cell lines integrin-linked N-oligosaccharides influence the VN adhesion receptor activity and function.
Źródło:
Acta Biochimica Polonica; 2010, 57, 1; 55-61
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Carbohydrate moieties of N-cadherin from human melanoma cell lines.
Autorzy:
Ciołczyk-Wierzbicka, Dorota
Gil, Dorota
Hoja-Łukowicz, Dorota
Lityńska, Anna
Laidler, Piotr
Powiązania:
https://bibliotekanauki.pl/articles/1043705.pdf
Data publikacji:
2002
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
melanoma
N-cadherin
N-glycans
Opis:
Expression of N-cadherin an adhesion molecule of the cadherin family, in tumor cells is associated with their increased invasive potential. Many studies suggested the role of N-linked oligosaccharides as important factors that contribute to metastasis by influencing tumor cell invasion and adhesion. N-cadherin is a heavily glycosylated protein. We have analysed the carbohydrate profile of this protein synthesized in human melanoma cell lines: WM35 from the primary tumor site and WM239, WM9, and A375 from different metastatic sites. N-cadherin was immunoprecipitated with anti-human N-cadherin polyclonal antibodies. Characterisation of its carbohydrate moieties was carried out by SDS/PAGE electrophoresis and blotting, followed by immunochemical identification of the N-cadherin polypeptides and analysis of their glycans using highly specific digoxigenin or biotin labelled lectins. The positive reaction of N-cadherin from the WM35 cell line with Galanthus nivalis agglutinin (GNA), Datura stramonium agglutinin (DSA) and Sambucus nigra agglutinin (SNA) indicated the presence of high-mannose type glycans and biantennary complex type oligosaccharides with α2-6 linked sialic acid. N-cadherin from WM239, WM9, and A375 cell lines gave a positive reaction with Phaseolus vulgaris leukoagglutinin (L-PHA) and lotus Tetragonolobus purpureas agglutinin (LTA). This indicated the presence of tri- or tetra-antennary complex type glycans with α-fucose. In addition, N-cadherin from WM9 (lymphomodus metastatic site) and A375 (solid tumor metastatic site) contained complex type chains with α2-3 sialic acid (positive reaction with Maackia amurensis agglutinin - MAA). The results demonstrated that N-glycans of N-cadherin are altered in metastatic melanomas in a way characteristic for invasive tumor cells.
Źródło:
Acta Biochimica Polonica; 2002, 49, 4; 991-998
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Hyaluronidase in human somatic tissues and urine: polymorphism and the activity in diseases
Autorzy:
Fiszer-Szafarz, Berta
Vannier, Pierre
Lityńska, Anna
Zouc, Lee
Czartoryska, Barbara
Tylki-Szymańska, Anna
Powiązania:
https://bibliotekanauki.pl/articles/1045277.pdf
Data publikacji:
1995
Wydawca:
Polskie Towarzystwo Biochemiczne
Źródło:
Acta Biochimica Polonica; 1995, 42, 1; 31-33
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Expression of β1-integrins and N-cadherin in bladder cancer and melanoma cell lines.
Autorzy:
Laidler, Piotr
Gil, Dorota
Pituch-Noworolska, Anna
Ciołczyk, Dorota
Książek, Dorota
Przybyło, Małgorzata
Lityńska, Anna
Powiązania:
https://bibliotekanauki.pl/articles/1044269.pdf
Data publikacji:
2000
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
cytofluorimetry
cancer
integrins
cadherins
cell lines
Opis:
Changes in the expression of integrins and cadherins might contribute to the progression, invasion and metastasis of transitional cell cancer of the bladder and of melanomas. The expression of α5 (P < 0.001), α2 and β1 (P < 0.05 - P < 0.001) integrin subunits in melanoma cells from noncutaneous metastatic sites (WM9, A375) were significantly increased as compared to cutaneous primary tumor (WM35) and metastatic (WM239) cell lines. These differences might be ascribed to the invasive character of melanoma cells and their metastasis to the noncutaneous locations. The significantly heterogeneous expression of β1 integrin subunit in two malignant bladder cancer cell lines (T24 and Hu456) and nonsignificant differences in the expression of α2, α3, and α5 subunits between malignant and non-malignant human bladder cell lines do not allow an unanimous conclusion on the role of these intergrin subunits in the progression of transitional cancer of bladder. The adhesion molecule, expressed in all studied melanoma and bladder cell lines, that reacted with anti-Pan cadherin monoclonal antibodies was identified as N-cadherin except in the HCV29 non-malignant ureter cell line. However, neither this nor any other bladder or melanoma cell line expressed E-cadherin. The obtained results imply that the replacement of E-cadherin by N-cadherin accompanied by a simultaneous increase in expression of a2, a3 and a5 integrin subunits clearly indicates an increase of invasiveness of melanoma and, to a lesser extent, of transitional cell cancer of bladder. High expression of N-cadherin and a5 integrin subunit seems to be associated with the most invasive melanoma phenotype.
Źródło:
Acta Biochimica Polonica; 2000, 47, 4; 1159-1170
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The structure of the oligosaccharides of α3β1 integrin from human ureter epithelium (HCV29) cell line.
Autorzy:
Lityńska, Anna
Pocheć, Ewa
Hoja-Łukowicz, Dorota
Kremser, Elżbieta
Laidler, Piotr
Amoresano, Angela
Monti, Chiara
Powiązania:
https://bibliotekanauki.pl/articles/1043787.pdf
Data publikacji:
2002
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
glycosylation
α3β1 integrin
cell line
MALDI MS
Opis:
There is a growing line of evidence that glycosylation of α and β subunits is important for the function of integrins. Integrin α3β1, from human ureter epithelium cell - line HCV29, was isolated by affinity chromatography on laminin GD6 peptide. Characterization of its carbohydrate moieties was carried out using sodium dodecyl sulfate/polyacrylamide gel electrophoresis followed by Western blotting on Immobilon P and on-blot deglycosylation with peptide N-glycosidase-F. Profiles of N-glycans for each subunit were obtained by matrix-assisted laser desorption/ionization mass spectrometry. Our findings demonstrated, in both subunits of integrin α3β1, the presence of complex type oligosaccharides with a wide heterogeneity. Bi- tri- and tetraantennary structures were the most common, while high-mannose type structures were minor. Also the presence of short poly-N-acetyllactosamine entities was shown. These results show that while the predominant oligosaccharides of both subunits are identical, some slight differences between them do exist.
Źródło:
Acta Biochimica Polonica; 2002, 49, 2; 491-500
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Presja gatunków obcych na lądowe ekosystemy Morskiej Antarktyki
Pressure of alien organisms on terrestrial ecosystems of Maritime Antarctic
Autorzy:
Chwedorzewska, Katarzyna
Korczak-Abshire, Małgorzata
Olech, Maria
Lityńska-Zając, Maria
Augustyniuk-Kram, Anna
Powiązania:
https://bibliotekanauki.pl/articles/1193221.pdf
Data publikacji:
2013
Wydawca:
Polskie Towarzystwo Przyrodników im. Kopernika
Opis:
Ze względu na geograficzną i historyczną izolację kontynentu antarktycznego odnotowano do tej pory jedynie sporadyczne pojawianie się na nim gatunków obcych. Jednak rosnąca antropopresja i gwałtowne ocieplanie się niektórych rejonów Antarktyki powodują, że ryzyko introdukcji gatunków obcych znacząco rośnie. Wraz z zaopatrzeniem stacji wiele propagul dociera do Antarktyki w dobrej kondycji. Agresywne, plastyczne organizmy, o dużym potencjale adaptacyjnym mogą szybko przystosować się do zmieniających się warunków klimatycznych, rozpoczynając kolonizację ocieplającej się Antarktyki. Wpływ takich organizmów na ekosystemy polarne jest w zasadzie niemożliwy do przewidzenia.
Due to geographic and historical isolation of the Antarctic continent, there are currently very few alien species introduc ed to the terrestrial ecosystems. However, due to increased man-made pressure and amelioration of climate in this region, the threat of the establishment of non-native species will grow markedly. Many diasporas can be quite easily, unintentionally transported in good condition to the Antarctic with research stations suppl ies. Flexible species are characterized by a very wide ecological amplitude, high adaptation capabilities and diverse way s of reproduction. They may thrive under harsh environmental condition s and colonize recipient ecosystem s. The impact of those organisms will be unpredictable.
Źródło:
Kosmos; 2013, 62, 3; 351-358
0023-4249
Pojawia się w:
Kosmos
Dostawca treści:
Biblioteka Nauki
Artykuł
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