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    Wyświetlanie 1-7 z 7
    Tytuł:
    Effects of storage in different semen extenders on the pre - freezing and post - thawing quality of boar spermatozoa
    Autorzy:
    Dziekonska, A.
    Zasiadczyk, L.
    Lecewicz, M.
    Strzezek, R.
    Koziorowska-Gilun, M.
    Fraser, R.
    Mogielnicka-Brzozowska, M.
    Kordan, W.
    Powiązania:
    https://bibliotekanauki.pl/articles/31403.pdf
    Data publikacji:
    2015
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Opis:
    The aim of this study was to investigate the effects of storage of semen in different commercial extenders on the pre-freezing and post-thawing quality of boar spermatozoa. Semen was diluted in BTS, Androhep (AH) and Gedil (GD), stored for 24 h at 17°C, and then frozen in accordance with the cryopreservation protocol. Analyses of the quality of spermatozoa included: motility, normal apical ridge (NAR) acrosome, plasma membrane integrity (PMI), mitochondrial membrane potential (MMP), measurements of ATP content and activity of superoxidase dismutase (SOD) and glutathione peroxidase (GPx). Prior to the freezing process, no significant effect of the extender on the sperm quality parameters was noted. After thawing the spermatozoa it was demonstrated that the type of extender used influenced PMI, MMP, ATP content and activity of GPx. In the AH extender the percentage of spermatozoa with PMI and ATP content in spermatozoa was significantly higher (P<0.05) as compared to the BTS or GD extenders. In addition, semen stored in the AH was characterised by a statistically higher (P<0.05) percentage of spermatozoa with MMP and increased activity of GPx as compared with the BTS. The results obtained indicate that for the cryopreservation process, boar spermatozoa stored for 24 hours in liquid state can be used. However, the type of extender used prior to freezing may have a significant effect on the post- thawing quality of the spermatozoa. The AH extender better secured the quality of thawed boar spermatozoa as compared with the BTS or GD.
    Źródło:
    Polish Journal of Veterinary Sciences; 2015, 18, 4
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Cybercartography and education: research and teaching with the Residential Schools Land Memory Atlas
    Autorzy:
    Pyne, Stephanie
    Taylor, D.R. Fraser
    Powiązania:
    https://bibliotekanauki.pl/articles/1191769.pdf
    Data publikacji:
    2020
    Wydawca:
    Oddział Kartograficzny Polskiego Towarzystwa Geograficznego
    Tematy:
    Cybercartography
    research
    teaching
    reconciliation
    reeflxivity
    emergence
    Indigenous Peoples
    Opis:
    This paper sheds light on intersections between teaching and research in the Cybercartographic Residential Schools Land Memory Atlas (RSLMA), which is the central output of the Residential Schools Land Memory Mapping Project (RSLMMP). Building on previous work in Cybercartography, the RSLMMP has further contributed to the integration of research and education and the emergence of new research and education relationships. Viewing the atlas as a project output comprised of iterative processes along multiple dimensions allows us to appreciate limitations as challenges for further iterations, including new related projects and ongoing volunteer work with students. In addition to participating in the national response to the Truth and Reconciliation Commission of Canada's Calls to Action, this project - including the atlas - provides a model for a unique blend of teaching and research and the basis for further and new collaborations with a variety of different partners, including Residential School survivors. As a reconciliation project, the Residential Schools Land Memory Atlas further contributes to the intercultural bridge building aims of its parent, the Lake Huron Treaty Atlas, as it forges on in new directions.
    Źródło:
    Polish Cartographical Review; 2020, 52, 3; 85-93
    2450-6974
    Pojawia się w:
    Polish Cartographical Review
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Effect of platelet activating factor (PAF) supplementation in semen extender on viability and ATP content of cryopreserved canine spermatozoa
    Autorzy:
    Kordan, W.
    Lecewicz, M.
    Strzezek, R.
    Dziekonska, A.
    Fraser, L.
    Powiązania:
    https://bibliotekanauki.pl/articles/30804.pdf
    Data publikacji:
    2010
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    platelet-activating factor
    supplementation
    semen
    viability
    spermatozoon
    cryopreservation
    cryopreserved spermatozoon
    dog
    adenosine triphosphate content
    sperm
    Opis:
    The aim of this study was to investigate the effect of platelet activating factor (PAF) on the quality characteristics of cryopreserved canine spermatozoa. Cryopreserved semen of 5 mixed-breed dogs was treated with different concentrations of exogenous PAF (1 × 10⁻³M, 1 × 10⁻⁴M, 1 × 10⁻⁵M and 1 × 10-6M) and examined at different time intervals (0, 30, 60 and 120 min). Cryopreserved semen treated without PAF was used as the control. Sperm quality was evaluated for motility (computer-assisted semen analysis, CASA), mitochondrial function (JC-1/PI assay) and plasma membrane integrity (SYBR-14/PI assay and Hoechst 33258). Also, ATP content of spermatozoa was determined using a bioluminescence assay. Treatment of cryopreserved semen with 1 × 10⁻³ M PAF at 120 min of incubation resulted in significantly higher total sperm motility compared with the control. It was observed that PAF-improved total sperm motility was concurrent with enhanced sperm motility patterns after treatment of cryopreserved semen. Treatment of cryopreserved semen with PAF did not improve either sperm mitochondrial function or plasma membrane integrity, as monitored by different fluorescent membrane markers. Furthermore, ATP content of cryopreserved spermatozoa was significantly higher when PAF was used at a concentration of 1 × 10⁻³ M compared with the control and other PAF treatments, regardless of the incubation time. The findings of this study indicated that treatment with 1 × 10⁻³ M PAF at 120 min of incubation rendered better quality of cryopreserved canine semen, which was associated with improved sperm motility parameters and ATP content. It can be suggested that exogenous PAF addition is beneficial as a supplement for canine semen extender used for cryopreservation.
    Źródło:
    Polish Journal of Veterinary Sciences; 2010, 13, 4
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Freezability and fertility of frozen-thawed boar semen supplemented with ostrich egg yolk lipoproteins
    Autorzy:
    Fraser, L.
    Zasiadczyk, Ł.
    Strzeżek, J.
    Strzeżek, R.
    Karpiesiuk, K.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087726.pdf
    Data publikacji:
    2018
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    boar
    egg yolk lipoproteins
    cryopreservation
    artificial insemination
    Źródło:
    Polish Journal of Veterinary Sciences; 2018, 21, 2; 255-263
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Applicability of 2,4-dinitrophenylhydrazine (DNPH) method of protein oxidative damage measurement in the seminal plasma of canine (Canis lupus familiaris) and stallion (Equus caballus)
    Autorzy:
    Mogielnicka-Brzozowska, M.
    Woźniak, M.J.
    Cichowska, A.W.
    Fraser, L.
    Kraziński, B.
    Strzezek, R.
    Zielińska, D.
    Powiązania:
    https://bibliotekanauki.pl/articles/16647556.pdf
    Data publikacji:
    2023
    Wydawca:
    Polska Akademia Nauk. Czasopisma i Monografie PAN
    Tematy:
    2,4-dinitrophenylhydrazine
    canine
    protein carbonylation
    seminal plasma
    stallion
    Opis:
    Seminal plasma (SP) proteins are responsible for sperm functional quality. Developing a reliable method to determine the degree of oxidative damage of these proteins is important for establishing semen fertilizing ability. The main aim of the study was to verify the applicability of protein carbonyl derivatives measurement in the SP of canine and stallion, using a method with 2,4-dinitrophenylhydrazine (DNPH). The research material consisted of ejaculates obtained from eight English Springer Spaniels, and from seven half-blood stallions during the breeding and non-breeding season. The content of carbonyl groups in the SP was measured on the basis of the reactions with DNPH. The following reagent variants were used to dissolve protein precipitates: Variant 1 (V1) – 6M Guanidine solution and Variant 2 (V2) – 0.1M NaOH solution. It has been shown that to obtain reliable results for the measurement of protein carbonylated groups in the dog and horse SP, both 6M Guanidine and 0.1M NaOH may be used. A correlation was also found between the number of carbonyl groups and the total protein content in the canine (V1: r = -0.724; V2: r = -0.847) and stallion (V1: r = -0.336; V2: r = -0.334) SP. Additionally, the study showed a higher content (p≤0.05) of protein carbonyl groups in the stallion SP in the non-breeding season compared to the breeding season. The method based on the reaction with DNPH, due to its simplicity and cost effectiveness, appears to be suitable for large-scale application in the determination of the SP proteins oxidative damage in dog and horse semen.
    Źródło:
    Polish Journal of Veterinary Sciences; 2023, 26, 2; 177-184
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Status of multi-wafer production MBE capabilities for extended SWIR III-V epi materials for IR detection
    Autorzy:
    Fraser, Everett D.
    Shao, Jiayi
    Frensley, Paul W.
    Barnes, Beau D.
    Clark, Kevin P.
    Kao, Yung-Chung
    Pinsukanjana, Paul R.
    Powiązania:
    https://bibliotekanauki.pl/articles/2204203.pdf
    Data publikacji:
    2023
    Wydawca:
    Polska Akademia Nauk. Stowarzyszenie Elektryków Polskich
    Tematy:
    extended shortwave
    GaSb
    InP
    detector
    pn-CBIRD
    Opis:
    The authors report two approaches, the first based on growth of lattice matched InGaAs/GaAsSb superlattice on InP substrate with tunable bandgap in the 2 to 3 μm range. The second approach is based on bulk random alloy InGaAsSb, which is tunable from 1.7 μm to 4.5 μm and lattice matched to the GaSb lattice constant. In each case, detector structures were fabricated and characterised. The authors have assessed the performance of these materials relative to commercially available extended short wave infrared devices through comparison to IGA-Rule 17 dark current performance level. A complementary barrier structure used in the InGaAsSb design showed improved quantum efficiency. The materials compare favourably to commercial technology and present additional options to address the challenging extended short wave infrared spectral band.
    Źródło:
    Opto-Electronics Review; 2023, 31, Special Issue; art. no. e144571
    1230-3402
    Pojawia się w:
    Opto-Electronics Review
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Semen quality assessments and their significance in reproductive technology
    Autorzy:
    Kordan, W.
    Fraser, L.
    Wysocki, P.
    Strzezek, R.
    Lecewicz, M.
    Mogielnicka-Brzozowska, M
    Dziekonska, A
    Soliwoda, D
    Koziorowska-Gilun, M
    Powiązania:
    https://bibliotekanauki.pl/articles/32408.pdf
    Data publikacji:
    2013
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Opis:
    Semen quality assessment methods are very important in predicting the fertilizing ability of persevered spermatozoa and to improve animal reproductive technology. This review discusses some of the current laboratory methods used for semen quality assessments, with references to their relevance in the evaluation of male fertility and semen preservation technologies. Semen quality assessment methods include sperm motility evaluations, analyzed with the computer-assisted semen analysis (CASA) system, and plasma membrane integrity evaluations using fluorescent stains, such as Hoechst 33258 (H33258), SYBR-14, propidium iodide (PI), ethidium homodimer (EthD) and 6-carboxyfluorescein diacetate (CFDA), and biochemical tests, such as the measurement of malondialdehyde (MDA) level. This review addresses the significance of specific fluorochromes and ATP measurements for the evaluation of the sperm mitochondrial status. Laboratory methods used for the evaluation of chromatin status, DNA integrity, and apoptotic changes in spermatozoa have been discussed. Special emphasis has been focused on the application of proteomic techniques, such as two-dimensional (2-D) gel electrophoresis and liquid chromatography mass spectrometry (LC-MS/MS), for the identification of the properties and functions of seminal plasma proteins in order to define their role in the fertilization-related processes.
    Źródło:
    Polish Journal of Veterinary Sciences; 2013, 16, 4
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-7 z 7

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