- Tytuł:
- In situ genetic variability and micropropagation of Cerastium banaticum (Rochel) Heuff. (Caryophyllaceae)– a rare and endemic species from Romania
- Autorzy:
-
Cristea, V.
Besenyei, E.
Jarda, L.
Farkas, A.
Marcu, D.
Clapa, D.
Halmagyi, A.
Butiuc-Keul, A. - Powiązania:
- https://bibliotekanauki.pl/articles/2117879.pdf
- Data publikacji:
- 2019
- Wydawca:
- Polska Akademia Nauk. Czasopisma i Monografie PAN
- Tematy:
-
acclimatization
biodiversity conservation
plant growth regulators
SSR markers - Opis:
- Rare and endemic plant species represent important components of plant biodiversity which require protection to ensure their sustainable conservation. Cerastium banaticum (Rochel) Heuff. is such an endemic and rare species from Romania, for which the genetic variability of two natural populations was studied by SSR markers. Shannon’s information index revealed low levels of genetic diversity in both populations (I = 0.296). As the first attempt in a conservation program a reproducible micropropagation protocol was established starting from seeds, followed by multiplication, rooting, and ex vitro acclimatization. Among the various plant growth regulators tested the highest multiplication coefficient was achieved on a culture medium with 0.5 mg L -1 6-furfurylaminopurine (K) and 1 mg L -1 α-naphthaleneacetic acid (NAA). On this PGRs concentration a number of 26.6 shoots/individual explant with a mean length of 7.9 cm for new generated shoots was registered. The highest number of roots/indi- vidual initiated shoot was 2.6 and it was recorded on a culture medium with 0.5 mg L -1 2-isopentyl-adenine (2iP) and 0.1 mg L -1 NAA. The outdoor acclimatization was successfully performed in a specially designed rocky area in the ‘Alexandru Borza’ Botanical Garden, Cluj-Napoca (Romania).
- Źródło:
-
Acta Biologica Cracoviensia. Series Botanica; 2019, 61, 1; 53-62
0001-5296 - Pojawia się w:
- Acta Biologica Cracoviensia. Series Botanica
- Dostawca treści:
- Biblioteka Nauki
Artykuł