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    Wyświetlanie 1-11 z 11
    Tytuł:
    Engineered resistance against proteinases
    Autorzy:
    Milner, Malgorzata
    Chroboczek, Jadwiga
    Zagorski-Ostoja, Wlodzimierz
    Powiązania:
    https://bibliotekanauki.pl/articles/1040935.pdf
    Data publikacji:
    2007
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    fusion proteins
    proteinase inhibitors
    protein protection
    Opis:
    Exogenous proteinase inhibitors are valuable and economically interesting protective biotechnological tools. We examined whether small proteinase inhibitors when fused to a selected target protein can protect the target from proteolytic degradation without simultaneously affecting the function and activity of the target domain. Two proteinase inhibitors were studied: a Kazal-type silk proteinase inhibitor (SPI2) from Galleria mellonella, and the Cucurbita maxima trypsin inhibitor I (CMTI I). Both inhibitors target serine proteinases, are small proteins with a compact structure stabilized by a network of disulfide bridges, and are expressed as free polypeptides in their natural surroundings. Four constructs were prepared: the gene for either of the inhibitors was ligated to the 5' end of the DNA encoding one or the other of two selected target proteins, the coat protein (CP) of Potato potyvirus Y or the Escherichia coli β-glucuronidase (GUS). CMTI I fused to the target proteins strongly hampered their functions. Moreover, the inhibitory activity of CMTI I was retained only when it was fused to the CP. In contrast, when fused to SPI2, specific features and functions of both target proteins were retained and the inhibitory activity of SPI2 was fully preserved. Measuring proteolysis in the presence or absence of either inhibitor, we demonstrated that proteinase inhibitors can protect target proteins used either free or as a fusion domain. Interestingly, their inhibitory efficiency was superior to that of a commercial inhibitor of serine proteinases, AEBSF.
    Źródło:
    Acta Biochimica Polonica; 2007, 54, 3; 523-536
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Virus-like particles of potato leafroll virus as potential carrier system for nucleic acids.
    Autorzy:
    Sułuja, Elżbieta
    Strokowskaja, Ludmiła
    Zagórski-Ostoja, Włodzimierz
    Pałucha, Andrzej
    Powiązania:
    https://bibliotekanauki.pl/articles/1041380.pdf
    Data publikacji:
    2005
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    encapsidation
    Virus-like particles
    Opis:
    Potato leafroll virus is a member of the polerovirus genus. The isometric virion is formed by a coat protein encapsidating single-stranded, positive-sense, mono-partite genomic RNA with covalently attached viral protein at the 5' end. The coat protein of the virus exists in two forms: i) a 23 kDa protein, the product of the coat protein gene, and ii) a 78 kDa protein, the product of the coat protein gene and an additional open reading frame expressed by read-through of the coat protein gene stop codon. The aim of this work was the expression of potato leafroll virus coat protein-based proteins that would be able to assemble into virus-like particles in insect cells. These modified particles were tested for their ability to encapsidate nucleic acids. Two types of N-terminally His-tagged coat protein constructs were used for the expression in insect cells: one, encoding a 23 kDa protein with the C-terminal amino-acid sequence corresponding to the wild type coat protein and the second with additional clathrin binding domain at the C-terminus. The expression of these two proteins by a recombinant baculovirus was characterized by Western immunoblotting with antibodies directed against potato leafroll virus. The protection or putative encapsidation of nucleic acids by these two coat protein derivatives was shown by DNase I and RNase A protection assays.
    Źródło:
    Acta Biochimica Polonica; 2005, 52, 3; 699-702
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Mapping of the influenza A hemagglutinin serotypes evolution by the ISSCOR method
    Autorzy:
    Radomski, Jan
    Słonimski, Piotr
    Zagórski-Ostoja, Włodzimierz
    Borowicz, Piotr
    Powiązania:
    https://bibliotekanauki.pl/articles/1039244.pdf
    Data publikacji:
    2014
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    ISSCOR descriptors
    phylogenetic analysis
    influenza virus
    hemagglutinin
    phylogenetic maps
    Opis:
    Analyses and visualizations by the ISSCOR method of influenza virus hemagglutinin genes of different A-subtypes revealed some rather striking temporal relationships between groups of individual gene subsets. Based on these findings we consider application of the ISSCOR-PCA method for analyses of large sets of homologous genes to be a worthwhile addition to a toolbox of genomics - allowing for a rapid diagnostics of trends, and ultimately even aiding an early warning of newly emerging epidemiological threats.
    Źródło:
    Acta Biochimica Polonica; 2014, 61, 3; 441-451
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Elements regulating Potato leafroll virus sgRNA1 translation are located within the coding sequences of the coat protein and read-through domain
    Autorzy:
    Łoniewska-Lwowska, Adrianna
    Chełstowska, Sylwia
    Zagórski-Ostoja, Włodzimierz
    Pałucha, Andrzej
    Powiązania:
    https://bibliotekanauki.pl/articles/1040473.pdf
    Data publikacji:
    2009
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    polerovirus
    subgenomic RNA
    in vitro translation
    Opis:
    Translation of viral proteins from subgenomic RNAs (sgRNAs) is a common strategy among positive-stranded RNA viruses. Unlike host mRNA, sgRNA of Potato leafroll virus (PLRV) does not possess a cap at its 5' end nor a poly(A) tail at the 3' terminus, both of which are known to be crucial for translation of RNA in eukaryotic cells. Here, we demonstrate, that in wheat germ extract (WGE) truncation of the sgRNA1 5' UTR increases translation efficiency, as it has previously been observed in rabbit reticulocyte lysate (RRL), whereas removal of the 3' UTR does not affect translation. We also describe two regulatory elements located within the coding sequence of the coat protein (CP) gene and its read-through domain (RTD) and are responsible for regulation of in vitro translation of the PLRV sgRNA1. The first element is composed of the purine sequence AAAGGAAA located between the AUG codons of the CP and 17K genes. Deletion of this domain or its substitution by pyrimidines reduced by half the translation of both genes, whereas deletion of the RTD resulted in a 3.6-fold reduction in translation efficiency. This is the first report of translation regulatory elements of plant viruses located within a coding region.
    Źródło:
    Acta Biochimica Polonica; 2009, 56, 4; 619-625
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Antibody response to DNA vaccine against H5N1 avian influenza virus in broilers immunized according to three schedules
    Autorzy:
    Stachyra, Anna
    Góra-Sochacka, Anna
    Zagórski-Ostoja, Włodzimierz
    Król, Ewelina
    Sirko, Agnieszka
    Powiązania:
    https://bibliotekanauki.pl/articles/1039267.pdf
    Data publikacji:
    2014
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    Avian influenza
    H5N1
    DNA vaccine
    broilers
    Opis:
    Broiler type chickens were immunized intramuscularly with a DNA vaccine encoding hemagglutinin (HA) from H5N1 avian influenza virus. The chickens were divided into four groups: control group which was not immunized, a group which obtained only one dose, and two groups which were immunized twice, one group with a boost two weeks after the priming and the other four weeks. Blood samples were collected at several time points and the dynamics of the humoral response to the vaccine was studied. High level of anti-HA antibodies was detected only in the last two groups, that is in chickens immunized according to the prime-boost strategy, regardless of the schedule. An additional interesting observation of this study was detection of the cross-reactivity of an anti-H5 HA positive serum with H5N2 and H1N1 viruses, suggesting that the DNA vaccine tested can induce antibodies of a broad specificity.
    Źródło:
    Acta Biochimica Polonica; 2014, 61, 3; 593-596
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Sprawy honorowe gen. Włodzimierza Ostoi-Zagórskiego w latach 1923–1928
    Autorzy:
    Kulka, Grzegorz
    Powiązania:
    https://bibliotekanauki.pl/articles/608408.pdf
    Data publikacji:
    2016
    Wydawca:
    Polska Akademia Nauk. Instytut Historii im. Tadeusza Manteuffla PAN w Warszawie
    Tematy:
    oficerski honor
    Sąd Honorowy dla Generałów
    gen. Włodzimierz Ostoja– Zagórski
    Opis:
    Affairs of Honour of General Włodzimierz Ostoja–Zagórski in 1923–1928The military career of General W. Ostoja–Zagórski, albeit not long (it ended with his mysterious disappearance in August 1927), brimmed with assorted events. Some of them, in particular those dating from the Polish Legions period, gave rise to controversies among political and officer circles since the General’s ambiguous stance considerably undermined his honesty, patriotism or honour. This article brings the reader closer to three affairs of honour pertaining to General Zagórski and examined by a Generals Court of Honour in 1923–1928. The procedure of these court trials not only demonstrates several decisive moments in his Army service and the attempt at explaining them made by the military administration of justice, but also the mechanisms of the functioning of Officers Courts of Honour. Almost the entire text is based on unpublished archival material.
    Źródło:
    Dzieje Najnowsze; 2016, 48, 3
    0419-8824
    Pojawia się w:
    Dzieje Najnowsze
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Expression of avian influenza haemagglutinin (H5) and chicken interleukin 2 (chIL-2) under control of the ptcB promoter in Lactococcus lactis
    Autorzy:
    Szatraj, Katarzyna
    Szczepankowska, Agnieszka
    Sączyńska, Violetta
    Florys, Katarzyna
    Gromadzka, Beata
    Łepek, Krzysztof
    Płucienniczak, Grażyna
    Szewczyk, Bogusław
    Zagórski-Ostoja, Włodzimierz
    Bardowski, Jacek
    Powiązania:
    https://bibliotekanauki.pl/articles/1039270.pdf
    Data publikacji:
    2014
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    Lactococcus lactis
    ptcB promoter
    heterologous gene expression
    avian influenza H5N1
    H5 haemagglutinin
    chicken interleukin-2
    Opis:
    Gram-positive and nonpathogenic lactic acid bacteria (LAB) are considered to be promising candidates for the development of new, safe systems of heterologous protein expression. Recombinant LAB has been shown to induce specific local and systemic immune response against selected pathogens, and could be a good alternative to classical attenuated carriers. The main goal of our study was to express the avian influenza haemagglutinin (H5) and chicken interleukin 2 (chIL-2) in Lactococcus lactis. Results of this study were anticipated to lead to construction of lactococcal strain(s) with potential vaccine properties against the avian influenza A (H5N1) virus. Expression of the cloned H5 gene, its His-tagged variant and chIL-2 gene, under the control of the ptcB gene promoter was attested by RT-PCR on transcriptional level and Western or dot blot analysis on translational level, demonstrating that system can be an attractive solution for production of heterologous proteins. The results of the preliminary animal trial conducted in mice are a promising step toward development of a vaccine against avian bird flu using Lactococcus lactis cells as antigen carriers.
    Źródło:
    Acta Biochimica Polonica; 2014, 61, 3; 609-614
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Expression, purification and characterization of glycosylated influenza H5N1 hemagglutinin produced in Pichia pastoris
    Autorzy:
    Kopera, Edyta
    Dwornyk, Angela
    Kosson, Piotr
    Florys, Katarzyna
    Sączyńska, Violetta
    Dębski, Janusz
    Cecuda-Adamczewska, Violetta
    Szewczyk, Bogusław
    Zagórski-Ostoja, Włodzimierz
    Grzelak, Krystyna
    Powiązania:
    https://bibliotekanauki.pl/articles/1039268.pdf
    Data publikacji:
    2014
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    Avian influenza
    H5N1
    recombinant hemagglutinin
    Pichia pastoris
    Opis:
    The A/swan/Poland/305-135V08/2006 (H5N1-subtype) hemagglutinin (HA) gene was cloned and expressed in yeast Pichia pastoris (P. pastoris). The HA cDNA lacking the C-terminal transmembrane anchor-coding sequence was fused to an α-factor leader peptide and placed under control of the methanol-inducible P. pastoris alcohol oxidase 1 (AOX1) promoter. Two P. pastoris strains: SMD 1168 and KM 71 were used for protein expression. Recombinant HA protein was secreted into the culture medium reaching an approximately 15 mg/L (KM 71 strain). Fusion protein with a His6 tag was purified to homogeneity in one step affinity chromatography. SDS-PAGE and MS/MS analysis indicated that the protein is cleaved into HA1 and HA2 domains linked by a disulfide bond. Analysis of the N-linked glycans revealed that the overexpressed HA is fully glycosylated at the same sites as the native HA in the vaccine strain. Immunological activity of the hemagglutinin protein was tested in mice, where rHA elicited a high immune response.
    Źródło:
    Acta Biochimica Polonica; 2014, 61, 3; 597-602
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-11 z 11

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