- Tytuł:
- In vitro Antileishmanial Activity of Hydroalcoholic Extract, Fractions, and Compounds Isolated from Leaves of Piper ovatum Vahl against Leishmania amazonensis
- Autorzy:
-
RODRIGUES-SILVA, Daniel
NAKAMURA, Celso Vataru
DIAS FILHO, Benedito Prado
UEDA-NAKAMURA, Tânia
CORTEZ, Diógenes Aparício Garcia - Powiązania:
- https://bibliotekanauki.pl/articles/763714.pdf
- Data publikacji:
- 2009
- Wydawca:
- Uniwersytet Jagielloński. Wydawnictwo Uniwersytetu Jagiellońskiego
- Tematy:
-
Antiprotozoan activity
ultrastructure alterations
Leishmania amazonensis
Piper ovatum - Opis:
- We assessed the biological activity of a crude extract, a mixture of several fractions, and a pure compound obtained from Piper ovatum Vahl against promastigote and amastigote forms of Leishmania amazonensis. The medicinal plant P. ovatum is used popularly as an anesthetic and anti-inflammatory. This study included the extraction process and bioassay-guided fractionation by the adsorption chromatography and Sephadex LH-20 method. A progressive increase in the antileishmanial effect was observed in the course of fractionation. The 50% inhibitory concentration (IC50) for dichloromethane-ethyl acetate (1:1 v/v) fraction was 2.1 μg/ml and 24 μg/ml; mixture of piperovatine: piperlongumune (2:3) 0.9 μg/ml and 24 μg/ml; piperovatine (1) 9.5 μg/ml and 10 μg/ml; and piperlonguminine (2) 2.5 μg/ml and 9.0 μg/ml, for promastigote and amastigote forms, respectively. Cytotoxicity analysis indicated that these toxic concentrations were much higher for J774G8 macrophages and Vero cells than for the protozoans. The mixture of piperovatine: piperlongumune (2:3) showed important antiprotozoal activity against the amastigote and promastigote forms of L. amazonensis, and it produced morphological changes in promastigotes and amastigotes at 0.9 μg/ml and 24 μg/ml (50% growth inhibition concentration), respectively, including intense cytoplasmic vacuolization, mitochondrial swelling, and mitochondrial damage, as revealed by transmission electron microscopy.
- Źródło:
-
Acta Protozoologica; 2009, 48, 1
0065-1583
1689-0027 - Pojawia się w:
- Acta Protozoologica
- Dostawca treści:
- Biblioteka Nauki
Artykuł