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    Wyświetlanie 1-9 z 9
    Tytuł:
    Zrodlo i znaczenie genetycznego zroznicowania populacji pasozytniczych pierwotniakow
    Autorzy:
    Majewska, A C
    Sulima, P.
    Powiązania:
    https://bibliotekanauki.pl/articles/837049.pdf
    Data publikacji:
    1999
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    zroznicowanie genetyczne
    Leishmania
    Giardia
    struktura populacji
    pierwotniaki
    parazytologia
    zroznicowanie gatunkowe
    Toxoplasma
    populacje zwierzat
    pasozyty
    Trypanosoma
    Cryptosporidium
    Opis:
    The application of biochemical and molecular techniques in parasitological studies has provided increasing evidences of genetic polymorphism among parasite populations. This review presents possible origins of genetic variation within populations of various protozoan species. Since the mode of reproduction has an important influence on genetic polymorphism within parasite populations these considerations refer mainly to some protozoan parasites which have various life cycles, e.g. Giardia, Trypanosoma, Cryptosporidium, Toxoplasma. Also other factors associated with parasites (such as: transmission and passage history in laboratory conditions; occurrence in different hosts or geographic regions; selective pressure of drugs; competitive interactions between populations) that affect parasite genetic diversity are discussed. However, the number of examined isolates of parasites and genetic markers, assortment of methods, probes, primers and reagents used is also of significance. The significance of genetic variability in parasite populations is still the subject of much interest and controversy. A simple interpretation of such variation is impossible because of the complexity of host-parasite interactions. The knowledge of parasite diversity at the nucleic acids level has continually increased, but a corect interpretation of this phenomenon requires at least the same knowledge of genetic variability in host populations. Nevertheless, genetic variability in protozoan parasites has many important implications, e.g. for taxonomy, epidemiology, control and evolution. Genetic differences within parasite populations might also be associated with phenotypic variability, e.g. virulence, antigenicity, infectivity, drug sensitivity, hostpreference etc.
    Źródło:
    Annals of Parasitology; 1999, 45, 3; 293-307
    0043-5163
    Pojawia się w:
    Annals of Parasitology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Zastosowanie technik biologii molekularnej w parazytologii: za i przeciw
    Autorzy:
    Majewska, A C
    Sulima, P.
    Powiązania:
    https://bibliotekanauki.pl/articles/840124.pdf
    Data publikacji:
    1998
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    parazytologia
    techniki badawcze
    techniki biologii molekularnej
    diagnostyka
    Opis:
    This review deals with possible advantages and disadvantages connected with applying molecular biology techniques in parasitology. It particularly concerns molecular diagnosis and epidemiology, as well as phylogenesis and taxonomy of selected parasite species.
    Źródło:
    Annals of Parasitology; 1998, 44, 2; 181-194
    0043-5163
    Pojawia się w:
    Annals of Parasitology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Źródło i znaczenie genetycznego zróżnicowania populacji pasożytniczych pierwotniaków
    SOURCE AND SIGNIFICANCE OF GENETIC POLYMORPHISM OF SELECTED PARASITIC PROTOZOA
    Autorzy:
    Majewska, A. C.
    Sulima, P.
    Powiązania:
    https://bibliotekanauki.pl/articles/2148795.pdf
    Data publikacji:
    1999
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    zroznicowanie genetyczne
    Leishmania
    Giardia
    struktura populacji
    pierwotniaki
    parazytologia
    zroznicowanie gatunkowe
    Toxoplasma
    populacje zwierzat
    pasozyty
    Trypanosoma
    Cryptosporidium
    Opis:
    The application of biochemical and molecular techniques in parasitological studies has provided increasing evidences of genetic polymorphism among parasite populations. This review presents possible origins of genetic variation within populations of various protozoan species. Since the mode of reproduction has an important influence on genetic polymorphism within parasite populations these considerations refer mainly to some protozoan parasites which have various life cycles, e.g. Giardia, Trypanosoma, Cryptosporidium, Toxoplasma. Also other factors associated with parasites (such as: transmission and passage history in laboratory conditions; occurrence in different hosts or geographic regions; selective pressure of drugs; competitive interactions between populations) that affect parasite genetic diversity are discussed. However, the number of examined isolates of parasites and genetic markers, assortment of methods, probes, primers and reagents used is also of significance. The significance of genetic variability in parasite populations is still the subject of much interest and controversy. A simple interpretation of such variation is impossible because of the complexity of host-parasite interactions. The knowledge of parasite diversity at the nucleic acids level has continually increased, but a corect interpretation of this phenomenon requires at least the same knowledge of genetic variability in host populations. Nevertheless, genetic variability in protozoan parasites has many important implications, e.g. for taxonomy, epidemiology, control and evolution. Genetic differences within parasite populations might also be associated with phenotypic variability, e.g. virulence, antigenicity, infectivity, drug sensitivity, hostpreference etc.
    Źródło:
    Wiadomości Parazytologiczne; 1999, 45, 3; 293-307
    0043-5163
    Pojawia się w:
    Wiadomości Parazytologiczne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Zastosowanie technik biologii molekularnej w parazytologii: za i przeciw
    APPLICATION OF MOLECULAR BIOLOGY TECHNIQUES IN PARASITOLOGY: FOR AND AGAINST
    Autorzy:
    Majewska, A. C.
    Sulima, P.
    Powiązania:
    https://bibliotekanauki.pl/articles/2148868.pdf
    Data publikacji:
    1998
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    parazytologia
    techniki badawcze
    techniki biologii molekularnej
    diagnostyka
    Opis:
    This review deals with possible advantages and disadvantages connected with applying molecular biology techniques in parasitology. It particularly concerns molecular diagnosis and epidemiology, as well as phylogenesis and taxonomy of selected parasite species.
    Źródło:
    Wiadomości Parazytologiczne; 1998, 44, 2; 181-194
    0043-5163
    Pojawia się w:
    Wiadomości Parazytologiczne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Ocena uzytecznosci metod wykrywania Cryptosporidium w kale ludzi i zwierzat
    Autorzy:
    Werner, A.
    Sulima, P.
    Majewska, A.C.
    Powiązania:
    https://bibliotekanauki.pl/articles/841199.pdf
    Data publikacji:
    2004
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    ocena przydatnosci
    kal
    metody wykrywania
    zwierzeta
    Cryptosporidium
    czlowiek
    Źródło:
    Annals of Parasitology; 2004, 50, 2; 209-220
    0043-5163
    Pojawia się w:
    Annals of Parasitology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Ocena użyteczności metod wykrywania Cryptosporidium w kale ludzi i zwierząt
    Evaluation of usefulness of different methods for detection of Cryptosporidium in human and animal stool samples
    Autorzy:
    Werner, A.
    Sulima, P.
    Majewska, A.C.
    Powiązania:
    https://bibliotekanauki.pl/articles/2146455.pdf
    Data publikacji:
    2004
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    ocena przydatnosci
    kal
    metody wykrywania
    zwierzeta
    Cryptosporidium
    czlowiek
    Opis:
    There are many methods for detection of Cryptosporidium oocysts. Most of them (more than 20) enable the microscopic detection of Cryptosporidium oocysts in faecal smears. Such a great variability of diagnostic methods may lead to confusion as far as the choice of an appropriate technique by a given laboratory is concerned. This study evaluated the diagnostic usefulness of Cryptosporidium oocysts and coproantigen detection methods in the diagnosis of cryptosporidiosis in human (266 stool specimen) and animals (205 from cattle, 160 from sheep, 30 from horses, 80 from cats, 227 from dogs and 11 from wild animals). The total number of human and animal stool specimens processed was 266 and 713, respectively. In this study the usefulness of several diagnostic methods was compared. The following techniques were taken into account: wet mounts, hematoxylin staining, four different specific methods (modified Zeihl-Neelsen, Kinyoun's, safraninmethylene blue, as well as carbol-methyl violet and tartrazyne) and commercially available kit based on enzyme-linked immunoassay (ProspecT(r) Cryptosporidium Microplate Assay). The final number of positive specimens was 123. Out of them 77 were positive in all specific methods. The oocysts found in stool specimens were measured. Humans were infected with C. parvum and animals with C. parvum, C. andersoni or C. felis. The statistical analysis has shown that EIA test was a better than microscopy method for identification of Cryptosporidium in faecal samples in human and wild animal. Sensitivity and specificity are important factors for the choice of a proper diagnostic method for Cryptosporidium detection, however other factors such as cost, simplicity and ease of interpretation of results are also important considerations.
    Źródło:
    Wiadomości Parazytologiczne; 2004, 50, 2; 209-220
    0043-5163
    Pojawia się w:
    Wiadomości Parazytologiczne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Kryptosporydioza u osób zakażonych HIV
    Autorzy:
    Majewska, A. C.
    Sulima, P.
    Werner, A.
    Barałkiewicz, G.
    Juszczyk, J.
    Pieniążek, N.J.
    Powiązania:
    https://bibliotekanauki.pl/articles/2148749.pdf
    Data publikacji:
    1999
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    kryptosporydioza
    choroby czlowieka
    wirus HIV
    parazytologia
    Opis:
    Cryptosporidium parvum is a coccidian parasite that affects millions of people worldwide. Clinical outcome of human cryptosporidiosis differs between immunocompetent and immunodeficient individuals. C. parvum is responsible for causing protracted and life-threatening diarrhea, biliary, and pulmonary infections in immunocompromised persons, especially in patients with AIDS. Though no effective treatment has been found so far, early diagnosis may be useful in controlling the infection. Thirty-eight stool specimens obtained from 35 HIV-positive patients admitted to the Clinic of Infectious Diseases in Poznań, Poland, were examined for the detection of oocysts, coproantigen and DNA of Cryptosporidium using standard microscopic, immunologic and molecular diagnostic methods. The presence of Cryptosporidium was detected in 10 HIV-positive patients. Oocysts, coproantigen and DNA of this parasite were identified solely in one specimen while Cryptosporidium DNA was detected in 8 specimens. Cryptosporidium coproantigen was found only in one sample. Although, the PCR was the most useful technique in the detection of Cryptosporidium in HIV-positive patients it should be noted that PCR has many pitfalls and needs to be carefully controlled to avoid both false positive and false negative results.
    Źródło:
    Wiadomości Parazytologiczne; 1999, 45, 2; 125-128
    0043-5163
    Pojawia się w:
    Wiadomości Parazytologiczne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Kryptosporydioza u osob zakazonych HIV
    Autorzy:
    Majewska, A C
    Sulima, P.
    Werner, A.
    Baralkiewicz, G.
    Juszczyk, J.
    Pieniazek, N.J.
    Powiązania:
    https://bibliotekanauki.pl/articles/839207.pdf
    Data publikacji:
    1999
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    kryptosporydioza
    choroby czlowieka
    wirus HIV
    parazytologia
    Opis:
    Cryptosporidium parvum is a coccidian parasite that affects millions of people worldwide. Clinical outcome of human cryptosporidiosis differs between immunocompetent and immunodeficient individuals. C. parvum is responsible for causing protracted and life-threatening diarrhea, biliary, and pulmonary infections in immunocompromised persons, especially in patients with AIDS. Though no effective treatment has been found so far, early diagnosis may be useful in controlling the infection. Thirty-eight stool specimens obtained from 35 HIV-positive patients admitted to the Clinic of Infectious Diseases in Poznań, Poland, were examined for the detection of oocysts, coproantigen and DNA of Cryptosporidium using standard microscopic, immunologic and molecular diagnostic methods. The presence of Cryptosporidium was detected in 10 HIV-positive patients. Oocysts, coproantigen and DNA of this parasite were identified solely in one specimen while Cryptosporidium DNA was detected in 8 specimens. Cryptosporidium coproantigen was found only in one sample. Although, the PCR was the most useful technique in the detection of Cryptosporidium in HIV-positive patients it should be noted that PCR has many pitfalls and needs to be carefully controlled to avoid both false positive and false negative results.
    Źródło:
    Annals of Parasitology; 1999, 45, 2; 125-128
    0043-5163
    Pojawia się w:
    Annals of Parasitology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    System PROJMAT 2001 z interfejsem do optymalizacji silników komutatorowych małej mocy
    System PROJMAT 2001 with technical data interface for optimisation of small commutator motors
    Autorzy:
    Puternicki, P.
    Sulima, R.
    Powiązania:
    https://bibliotekanauki.pl/articles/159261.pdf
    Data publikacji:
    2002
    Wydawca:
    Sieć Badawcza Łukasiewicz - Instytut Elektrotechniki
    Tematy:
    maszyny komutatorowe
    silniki komutatorowe
    optymalizacja
    interfejs
    Opis:
    Opracowano system PROJMAT 2001, umożliwiający deterministyczną optymalizację silników komutatorowych małej mocy. Pracuje on w środowisku Matlab 5.0 z pakietem Toolbox Optimisation. W artykule podano ogólne sformułowanie matematyczne, deterministycznej, wielokryterialnej optymalizacji silników komutatorowych wraz ze sformułowaniami szczegółowymi dla trzech kryteriów. Opracowano zintegrowany z systemem interfejs komunikacji z użytkownikiem. Przedstawiono strukturę interfejsu oraz podkreślono nadrzędną jego funkcję w całym systemie PROJMAT 2001. W artykule przedstawiono również szczegółowy opis dodatkowych funkcji interfejsu służących projektantowi do łatwego i szybkiego przygotowania danych wejściowych i ograniczeń oraz przeprowadzenia obliczeń optymalizacyjnych i projektowych maszyny. Podano przykładowe wyniki optymalizacji i projektowania oraz analizy zbieżności optymalnych wyników uzyskiwanych z obliczeń z różnych punktów startowych. Uzyskana zbieżność świadczy o możliwości traktowania optimum lokalnego jako bardzo bliskiego optimum globalnego.
    System PROJMAT 2000 is used for designing small commutator motors. The software is used for multicriterial optimisation in the Toolbox Optimisation package. To facilitate communication with the user an interface has been added to the system PROJMAT 2000 forming the PROJMAT 2001 system. The interface is constructed of elements and functions of the Matlab 5.0 in the Uitools package. The system is built on the basis of a series of windows which make possible segregation and communication with the user. It gives a possibility of an easy and comprehensible for the designer preparation of input data, constraints and enables optimisation and design computations for machines to be carried out. The internal structure of the PROJMAT 2001 system is shown in Fig. 1. The main window of the interface is shown in Fig. 2. The interface has a series of functions to facilitate the use of the calculation program. It has a series of "function-windows" (PROJEKT - the catalogue of design, DANE - technical data, KRZYWE - magnetisation characteristics, OGR.PROSTE - simple constraints, - UWIKŁANE - implicit constraints, POMOCNE - tables for calculations, WYNIKI - results). The interface makes possible the edition (press the keys: EDYTUJ), correction and saving of files selected by the user. The interface facilitates the data preparation. The order of data preparation for calculation is shown in Fig.3...8. Fig.3 shows the window with technical data. System PROJMAT 2001 makes it possible to see the magnetisation characteristic; it is shown in Fig.4 and Fig.5. The next data for calculation are simple constraints (Fig.6). The table of implicit constraints is shown in Fig.7, whilst the window for making save it - in Fig.8 The optimisation process can be started just after preparation of the set of files described in the main file. The PROJMAT 2001 system has an interface and two independently operating calculation programs: the optimisation (OPTYMALIZACJA) and the postprocessor (PROJEKT) programs. The final design vector is obtained as a result of the system optimisation calculations. The values of the initial vector input from the table (Fig.9) and ranking of criteria (Fig.11) are necessary to carry out these calculations. The postprocessor carries out the design calculations in two variants (Fig.12): PROJEKT WYJŚCIOWY - uses an arbitrary vector of decisive variables entered by the user, PROJEKT OPTYMALNY - uses the final design vector calculated in optimisation process. In addition the user can describe the design carried out (Fig.13). The calculation result is automatically recorded in the form of the *.dat file in the "WYNIKI directory (Fig.14). Description and a list of m-files names used for calculating the given design is automatically added to the result file (see example of calculation). All files indispensable for calculation of a given design are saved in corresponding catalogues. The set of designs saved in the catalogue "WYNIKI" creates the base of knowledge. The saved design facilitate analyses and comparisons of machines of a given type. An example of the results of motor design and an example of convergence of the local- and the global optimum of the objective function are given in the paper (see Fig. 15 & 16).
    Źródło:
    Prace Instytutu Elektrotechniki; 2002, 210; 105-133
    0032-6216
    Pojawia się w:
    Prace Instytutu Elektrotechniki
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-9 z 9

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