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Wyświetlanie 1-15 z 26
Tytuł:
Przydatnosc metod analizy genomu w diagnostyce giardiozy i kryptosporydiozy
Autorzy:
Kasprzak, W
Majewska, A.C.
Powiązania:
https://bibliotekanauki.pl/articles/840681.pdf
Data publikacji:
1995
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
kryptosporydioza
choroby inwazyjne
parazytologia
genomy
diagnostyka
giardioza
metody analizy
Opis:
The article comprises a critical review on practical applications of molecular technology in parasitological diagnostics in a broad sense, also as a diagnosis of species and a method of epidemiological analysis. Techniques of genome analysis at different levels, as specific nucleic acid probes, DNA restriction profiles (RFLP), hybridization techniques, pulse-field gel electrophoresis, in vitro nucleic acid amplification, and DNA fingerprint technique used in studies on Giardia and Cryptosporidium were discussed. The essential reservation as far as this technology is concerned refers to its usefulness in parasitological diagnostics; there is no sense in working out methods for recognizing parasites which could otherwise be identified by well trained parasitologists and simple microscopic methods. The improved diagnosis of parasites resulting from the application of molecular technology significantly contributed to the armentarium of parasitologists. Application of recent molecular technology in diagnosis of giardiosis and cryptosporidiosis may basically support clinical diagnosis which provides possibilities of early and selective treatment and makes possible epidemiological studies. These assays will permit not only a rapid diagnosis and exact differentiation but will also enable a better recognition of Giardia and Cryptosporidium genome organization. However, in spite of the wide availability of this new techniques they have not been fully applied - as yet – in diagnosis and in epidemiological studies on these parasites. The authors share the opinion of BUSCH (1991) on the need of proper recognition of high-quality and rigorous work in employing new molecular assays, because their wide availability and high sensitivity could cause "false-positive" results by contamination with amplified DNA sequences. They totally support the conclusion made by THOMPSON and MELONI (1993) that the molecular data must be fairly interpreted by collaborating molecular biologists, parasitologists, and epidemiologists - and clinicians as well - to avoid forming of a confusing picture of genetic diversity of the parasites with no practical application.
Źródło:
Annals of Parasitology; 1995, 41, 1; 3-11
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Przydatność metod analizy genomu w diagnostyce giardiozy i kryptosporydiozy
APPLICATION OF MOLECULAR BIOLOGY IN DIAGNOSIS OF GIARDIOSIS AND CRYPTOSPORIDIOSIS
Autorzy:
Kasprzak, W.
Majewska, A.C.
Powiązania:
https://bibliotekanauki.pl/articles/2151375.pdf
Data publikacji:
1995
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
kryptosporydioza
choroby inwazyjne
parazytologia
genomy
diagnostyka
giardioza
metody analizy
Opis:
The article comprises a critical review on practical applications of molecular technology in parasitological diagnostics in a broad sense, also as a diagnosis of species and a method of epidemiological analysis. Techniques of genome analysis at different levels, as specific nucleic acid probes, DNA restriction profiles (RFLP), hybridization techniques, pulse-field gel electrophoresis, in vitro nucleic acid amplification, and DNA fingerprint technique used in studies on Giardia and Cryptosporidium were discussed. The essential reservation as far as this technology is concerned refers to its usefulness in parasitological diagnostics; there is no sense in working out methods for recognizing parasites which could otherwise be identified by well trained parasitologists and simple microscopic methods. The improved diagnosis of parasites resulting from the application of molecular technology significantly contributed to the armentarium of parasitologists. Application of recent molecular technology in diagnosis of giardiosis and cryptosporidiosis may basically support clinical diagnosis which provides possibilities of early and selective treatment and makes possible epidemiological studies. These assays will permit not only a rapid diagnosis and exact differentiation but will also enable a better recognition of Giardia and Cryptosporidium genome organization. However, in spite of the wide availability of this new techniques they have not been fully applied - as yet – in diagnosis and in epidemiological studies on these parasites. The authors share the opinion of BUSCH (1991) on the need of proper recognition of high-quality and rigorous work in employing new molecular assays, because their wide availability and high sensitivity could cause "false-positive" results by contamination with amplified DNA sequences. They totally support the conclusion made by THOMPSON and MELONI (1993) that the molecular data must be fairly interpreted by collaborating molecular biologists, parasitologists, and epidemiologists - and clinicians as well - to avoid forming of a confusing picture of genetic diversity of the parasites with no practical application.
Źródło:
Wiadomości Parazytologiczne; 1995, 41, 1; 3-11
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Wirusy pasozytniczych pierwotniakow
Autorzy:
Kasprzak, W
Majewska, A.C.
Powiązania:
https://bibliotekanauki.pl/articles/838835.pdf
Data publikacji:
1995
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
wirusy
pierwotniaki
parazytologia
pasozyty
Opis:
The authors present the actual review on several publications concerning the molecular characterizations of the viruses found in parasitic protozoa such as Giardia, Trichomonas, Leishmania and Entamoeba histolytica. All of the RNA viruses observed in parasitic protozoa showed several similarities and did not considerably differ from the viruses found in simple eukaryotic cells; they closely correspond to dsRNA viruses of yeast. The supposition that the protozoan symbionts detected in laboratories transfer to their hosts in natural conditions seemed to be rational, though, there are no evidences that these symbionts are potential pathogens. However, the opinion reiterates that intestinal protozoa (e.g. Entamoeba histolytica) may serve as vectors for HIV or cofactors of HIV infection. The authors point out that irrespective of the potential role of viruses as vectors in the transfection system for parasitic protozoa, the observed viral system constitutes an unusual experimental system to solve the problems of gene expression.
Źródło:
Annals of Parasitology; 1995, 41, 2; 131-137
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Kryptosporydioza - parazytoza o typowym uwarunkowaniu srodowiskowym
Autorzy:
Kasprzak, W
Majewska, A.C.
Powiązania:
https://bibliotekanauki.pl/articles/840071.pdf
Data publikacji:
1995
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
kryptosporydioza
choroby inwazyjne
pasozyty zwierzat
kryptosporidia
parazytologia
Cryptosporidium
pasozyty czlowieka
Opis:
The article reviews the problems on epidemiology of cryptosporidiosis and gives a detailed description of the massive waterborne outbreak in Milwaukee (Wisconsin, USA) associated with a break in filtration capacity of a public water supply. The authors emphasize the need for high-quality diagnostic procedures as current coprodiagnostic microscopical techniques seemed to fail to detect Cryptosporidium oocysts.
Źródło:
Annals of Parasitology; 1995, 41, 2; 149-154
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Efektywność metod określania żywotności cyst Giardia
Efficacy of methods assessing the viability of Giardia cysts
Ehffektivnost' metodov opredelenija vital'nosti cist Giardia
Autorzy:
Kasprzak, W.
Majewska, A.C.
Powiązania:
https://bibliotekanauki.pl/articles/2152702.pdf
Data publikacji:
1987
Wydawca:
Polskie Towarzystwo Parazytologiczne
Źródło:
Wiadomości Parazytologiczne; 1987, 33, 2; 147-156
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-15 z 26

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