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Wyświetlanie 1-15 z 42
Tytuł:
Alternative therapies in Staphylococcus aureus diseases
Autorzy:
Kurlenda, Julianna
Grinholc, Mariusz
Powiązania:
https://bibliotekanauki.pl/articles/1039729.pdf
Data publikacji:
2012
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
Staphylococcus aureus diseases
alternative therapies
Opis:
Staphylococcus aureus is a common pathogen responsible for health-care-associated infections as well as community acquired ones. It is the etiological factor of a wide spectrum of infections. Therapeutic problems are caused by resistance of S. aureus to many antibiotics, specifically to methicillin (methicillin-resistant S. aureus, MRSA). In such cases a limited spectrum of antibiotics may be used and prolonged hospitalization is costly. Hence, there is an urgent need for the development of alternative antibiotic therapeutics. This work reviews the current knowledge concerning prospective treatment of staphylococcal diseases.
Źródło:
Acta Biochimica Polonica; 2012, 59, 2; 171-184
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Differences in metabolic profiles of planktonic and biofilm cells in Staphylococcus aureus - 1H Nuclear Magnetic Resonance search for candidate biomarkers
Autorzy:
Junka, Adam
Deja, Stanisław
Smutnicka, Danuta
Szymczyk, Patrycja
Ziółkowski, Grzegorz
Bartoszewicz, Marzenna
Młynarz, Piotr
Powiązania:
https://bibliotekanauki.pl/articles/1039470.pdf
Data publikacji:
2013
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
Staphylococcus aureus
biofilm
NMR
metabolomics
glycine-betaine
Opis:
Staphylococcus aureus is responsible for many types of infections related to biofilm presence. As the early diagnostics remains the best option for prevention of biofilm infections, the aim of the work presented was to search for differences in metabolite patterns of S. aureus ATCC6538 biofilm vs. free-swimming S. aureus planktonic forms. For this purpose, Nuclear Magnetic Resonance (NMR) spectroscopy was applied. Data obtained were supported by means of Scanning Electron Microscopy, quantitative cultures and X-ray computed microtomography. Metabolic trends accompanying S. aureus biofilm formation were found using Principal Component Analysis (PCA). Levels of isoleucine, alanine and 2,3-butanediol were significantly higher in biofilm than in planktonic forms, whereas level of osmoprotectant glycine-betaine was significantly higher in planktonic forms of S. aureus. Results obtained may find future application in clinical diagnostics of S. aureus biofilm-related infections.
Źródło:
Acta Biochimica Polonica; 2013, 60, 4; 701-706
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Antibiofilm activity of mushroom extracts against Staphylococcus aureus
Autorzy:
Čuvalová, Anna
Strapáč, Imrich
Handrová, Livia
Kmeť, Vladimir
Powiązania:
https://bibliotekanauki.pl/articles/2183555.pdf
Data publikacji:
2018-12-31
Wydawca:
Uniwersytet Pedagogiczny im. Komisji Edukacji Narodowej w Krakowie
Tematy:
biofilm
fungi
ixodid ticks (Acari)
milk
Staphylococcus aureus
Opis:
Mushrooms are a renowned source of products with an array of bioactivities, from antibacterial to antiviral, cytotoxic, antifeeding, antifungal or antioxidant and might be a valuable resource in the search of new bioactive extracts to inhibit biofilm production. We demonstrate the effect of five mushroom water extracts Macrolepiota procera, Pleurotus ostreatus, Auricularia auricula-judae, Armillaria mellea and Laetiporus sulphurous on biofilm formation of four Staphylococcus aureus strains isolated from ixodid ticks (Acari) and ewe´s milk. The PCR was used for detection of virulence genes (hla, isdA, B, bbp, sirB, fnbpA, sdrE, agr II). The ability of biofilm formation and anti-biofilm activity of mushrooms extracts was assessed in a quantitative crystal violet assay. The biofilm formation of S. aureus strains was significantly reduced by all mushrooms extracts (p < 0.001). We showed that more significant anti-biofilm effect of the extracts was of Staphylococcus aureus isolated from ixodid ticks in comparison to Staphylococcus aureus isolated from ewe´s milk. In the present study, A. mellea, P. ostreatus, L. sulphurous, A. auricula-judae and M. procera extracts inhibited biofilm formation by 70.87%, 67.00%, 64.14%, 62.77% and 47.71%, respectively. The results suggest that compounds in mushrooms extracts might be useful to control and handle detrimental infections caused by animal and human pathogens.
Źródło:
Annales Universitatis Paedagogicae Cracoviensis Studia Naturae; 2018, 3 (suppl.); 17-23
2543-8832
2545-0999
Pojawia się w:
Annales Universitatis Paedagogicae Cracoviensis Studia Naturae
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Antibiofilm activity against Staphylococcus aureus and content analysis of Taraxacum Officinale phenolic extract
Autorzy:
Xu, P.
Xu, X.B.
Khan, A.
Fotina, T.
Wang, S.H.
Powiązania:
https://bibliotekanauki.pl/articles/2087162.pdf
Data publikacji:
2021
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Taraxacum Officinale
phenolic
antibacterial mechanism
HPLC
Staphylococcus aureus
Źródło:
Polish Journal of Veterinary Sciences; 2021, 24, 2; 243-251
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Nasal carriage of various staphylococcal species in small ruminant lentivirus-infected asymptomatic goats
Autorzy:
Moroz, A.
Szaluś-Jordanow, O.
Czopowicz, M.
Brodzik, K.
Petroniec, V.
Augustynowicz-Kopeć, E.
Lutyńska, A.
Roszczynko, M.
Gołoś-Wójcicka, A.
Korzeniowska-Kowal, A.
Gamian, A.
Mickiewicz, M.
Frymus, T.
Petelicka, H.
Kaba, J.
Powiązania:
https://bibliotekanauki.pl/articles/2087322.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
caprine arthritis-encephalitis
MALDI-TOF
PFGE
Staphylococcus aureus
Źródło:
Polish Journal of Veterinary Sciences; 2020, 23, 2; 203-209
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The study on bactericidal effect and ultrastructural alterations of chlorocresol nanoemulsion disinfectant against Staphylococcus aureus
Autorzy:
Zhang, Y.F.
Sun, Y.W.
Liu, X.H.
An, Z.X.
Yang, X.F.
Powiązania:
https://bibliotekanauki.pl/articles/16647452.pdf
Data publikacji:
2023
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
chlorocresol
nanoemulsion
disinfectant
Staphylococcus aureus
bactericidal effect
ultrastructure
Opis:
Chlorocresol nanoemulsion disinfectant (CND) is an environmental disinfectant prepared with nanoemulsion as its drug carrier. This study aimed to investigate the bactericidal effect of CND on Staphylococcus aureus ( S. aureus) and its effect on bacterial ultrastructure. The neutralizing effect of CND against S. aureus was first screened by suspension quantitative evaluation experiment procedure of neutralizer. Disinfection performance was evaluated by the determination of Minimal Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC), quantitative bactericidal experiment, and comparative experiment of disinfection performance between 0.1% CND and 0.1% chlorocresol aqueous solution. Meanwhile, the effect of CND on the ultrastructure of S. aureus was investigated with scanning electron microscope (SEM) and transmission electron microscope (TEM) to preliminarily explore the bactericidal mechanism. The results showed that 3% Tween-80 in PBS could be screened as the neutralizer of CND against S. aureus. MIC and MBC were 100 μg/mL and 200 μg/mL, respectively. The bactericidal rates were all 100% when 0.06% and 0.08% disinfectant acted for 15 and 5 min, respectively. Furthermore, compared with 0.1% chlorocresol aqueous solution, the bactericidal effect of 0.1% CND was significantly enhanced (p<0.01). After treatment with CND for 10 min, SEM observation showed that the morphology of S. aureus cells were changed and the integrity destroyed. TEM observation showed that the cell shape changed, and the structures of the cell wall, cell membrane and cytoplasm were damaged in varying degrees. CND showed the strong bactericidal effect on S. aureus and could cause ultrastructure alterations of S. aureus.
Źródło:
Polish Journal of Veterinary Sciences; 2023, 26, 1; 13-20
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Leonurus cardiaca L. herb - a derived extract and an ursolic acid as the factors affecting the adhesion capacity of Staphylococcus aureus in the context of infective endocarditis
Autorzy:
Micota, Bartłomiej
Sadowska, Beata
Podsędek, Anna
Redzynia, Małgorzata
Różalska, Barbara
Powiązania:
https://bibliotekanauki.pl/articles/1039309.pdf
Data publikacji:
2014
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
ECM
Leonurus cardiaca
ursolic acid
Staphylococcus aureus
Opis:
The objective was an assessment of the impact of Leonurus cardiaca L. extract (LCE) and ursolic acid (UA) on the adhesive properties of Staphylococus aureus NCTC 8325 strain, expressing virulence factors important in the pathogenesis of infective endocarditis. The adhesion and biofilm formation of bacteria cultured in the presence of subinhibitory concentrations of LCE or UA on the abiotic surface or covered with fibrinogen, fibronectin or collagen, were evaluated. Inhibitory effects of LCE and UA on staphylococcal adherence to both types of surface were demonstrated. This, in the case of UA, resulted in a significant reduction of biofilm formation.
Źródło:
Acta Biochimica Polonica; 2014, 61, 2; 385-388
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Proteomics in studies of Staphylococcus aureus virulence
Autorzy:
Bonar, Emilia
Wójcik, Iwona
Wladyka, Benedykt
Powiązania:
https://bibliotekanauki.pl/articles/1038964.pdf
Data publikacji:
2015
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
Staphylococcus aureus
proteomics
virulence factors
secretome
surfacome
Opis:
Staphylococcus aureus is a widespread, opportunistic pathogen that causes community and hospital acquired infections. Its high pathogenicity is driven by multifactorial and complex mechanisms determined by the ability of the bacterium to express a wide variety of virulence factors. The proteome secreted into extracellular milieu is a rich reservoir of such factors which include mainly nonenzymatic toxins and enzymes. Simultaneously, membrane proteins, membrane-cell wall interface proteins and cell wall-associated proteins also strongly influence staphylococcal virulence. Proteomics shows a great potential in exploring the role of the extracellular proteome in cell physiology, including the pathogenic potential of particular strains of staphylococci. In turn, understanding the bacterial physiology including the interconnections of particular factors within the extracellular proteomes is a key to the development of the ever needed, novel antibacterial strategies. Here, we briefly overview the latest applications of gel-based and gel-free proteomic techniques in the identification of the virulence factors within S. aureus secretome and surfacome. Such studies are of utmost importance in understanding the host-pathogen interactions, analysis of the role of staphylococcal regulatory systems and also the detection of posttranslational modifications emerging as important modifiers of the infection process.
Źródło:
Acta Biochimica Polonica; 2015, 62, 3; 367-381
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Isolation of vanconycin resistant Staphylococcus aureus from the wounds of hospital patients in Uyo, Akwa Ibom State, Nigeria
Autorzy:
Anosike, I. K.
Ebana, R. U. B.
Edet, U. O.
Umoh, I. S.
Powiązania:
https://bibliotekanauki.pl/articles/1076716.pdf
Data publikacji:
2019
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:
Methicillin
Staphylococcus aureus
Vancomycin
Wounds
antibiotic resistance
Opis:
Staphylococcus aureus is a very important human pathogen that generates a number of human infections. Isolation of vancomycin-resistant Staphylococcus aureus (VRSA) was carried out from wound samples of patients attending University of Uyo Teaching Hospital, Akwa Ibom state, Nigeria. A total of 45 wound samples from 15 patients were collected aseptically in triplicate, using sterile cotton swabs moistened in sterile normal saline. Microbiological analysis and susceptibility to vancomycin, including minimum inhibitory and minimum bactericidal concentrations (MIC and MBC), were carried out using standard methodologies. A total of 15 isolates were obtained and these showed varying MIC and MBC patterns. Out of the 15 S. aureus isolated, only one isolate had an MIC of approximately 4 µg/ml, while twelve isolates gave MIC values that ranged from 15.62 µg/ml to 250 µg/ml. The remaining 2 isolates gave MIC values that were ≥ 500 µg/ml. These 2 isolates exhibited alpha haemolysis on blood agar, unlike the others that were beta haemolytic. The results of the MBC also showed variations amongst the isolates. A total of 10 isolates gave MBC values that ranged from 62.5 µg/ml to 500 µg/ml, while the remaining 5 isolates gave MBC values that were ≥ 500 µg/ml. The high MIC and MBC values obtained showed that vancomycin-resistant Staphylococcus aureus is increasing at an alarming rate, and this accounts for the gradual decline in the effectiveness associated with the use of vancomycin. Given the widespread prevalence of VRSA, there is a need for newer therapeutics that can reverse this surge.
Źródło:
World News of Natural Sciences; 2019, 24; 134-144
2543-5426
Pojawia się w:
World News of Natural Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Antibacterial activity of covered paper after storage
Autorzy:
Jarosz, Michał
Sumińska, Patrycja
Kowalska, Urszula
Mizielińska, Małgorzata
Powiązania:
https://bibliotekanauki.pl/articles/1112792.pdf
Data publikacji:
2018
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:
Staphylococcus aureus
active coatings
covered paper
polylysine
Opis:
The paper covered with a hydrophobic Topscreen coating was coated with the second layer. The goal of paper covering was to obtain the antimicrobial properties of the external coating. The samples were stored 2 months at 20 ºC. The influence of storage on the antimicrobial properties of the external coating was analyzed. The results of the study showed that paper covered with Topscreen coating did not have an influence on the growth of Staphylococcus aureus cells. The second (external) layer containing 2% polylysine as an active substance decreased the growth of S. aureus. The 2 months storage of the covered paper did not influence the antimicrobial properties of coating with polylysine against S. aureus. It was demonstrated that paper covered with hydrophobic coating had no influence on the growth of E. coli cells as well. In this case the influence of 2 months storage on the antimicrobial properties of the coating with polylysine was observed. In contrast to the results obtained for the samples that were not store, the decrease of the growth of the bacterial cells after 24 h contact with a hydrophobic coating devoid of an active substance was noticed.
Źródło:
World News of Natural Sciences; 2018, 17; 141-146
2543-5426
Pojawia się w:
World News of Natural Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Production of bioactive metabolites from different marine endophytic Streptomyces species and testing them against methicillin-resistant Staphylococcus aureus (MRSA) and cancer cell lines
Autorzy:
El-Gendy, M.M.A.A.
Mohamed, Z.K.
Hekal, N.Z.
Ali, F.M.
Yousef, A.E.M.
Powiązania:
https://bibliotekanauki.pl/articles/81078.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Staphylococcus aureus
infection
methicillin-resistant Staphylococcus aureus
endophyte
Streptomyces
bioactive metabolite
Actinomycetes
marine invertebrate
anticancer activity
cancer cell line
lung cancer
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2018, 99, 1
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Self-reported methicillin-resistant Staphylococcus aureus infection in USA pork producers
Autorzy:
Leedom, Larson K R
Smith, T.C.
Donham, K.J.
Powiązania:
https://bibliotekanauki.pl/articles/51288.pdf
Data publikacji:
2010
Wydawca:
Instytut Medycyny Wsi
Tematy:
pork production
pork producer
USA
animal farm
pig
agricultural worker
human disease
infection
methicillin-resistant Staphylococcus aureus
Staphylococcus aureus
Źródło:
Annals of Agricultural and Environmental Medicine; 2010, 17, 2; 331-334
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Staphylococcus aureus as an infectious agent: overview of biochemistry and molecular genetics of its pathogenicity
Autorzy:
Plata, Konrad
Rosato, Adriana
Węgrzyn, Grzegorz
Powiązania:
https://bibliotekanauki.pl/articles/1040468.pdf
Data publikacji:
2009
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
Staphylococcus aureus
virulence
pathogenicity genes
toxins
methicillin resistance
Opis:
Although it is estimated that 20-30% of the general human population are carriers of Staphylococcus aureus, this bacterium is one of the most important etiological agents responsible for healthcare-associated infections. The appearance of methicillin resistant S. aureus (MRSA) strains has created serious therapeutical problems. Detailed understanding of the mechanisms of S. aureus infections seems necessary to develop new effective therapies against this pathogen. In this article, we present an overview of the biochemical and genetic mechanisms of pathogenicity of S. aureus strains. Virulence factors, organization of the genome and regulation of expression of genes involved in virulence, and mechanisms leading to methicilin resistance are presented and briefly discussed.
Źródło:
Acta Biochimica Polonica; 2009, 56, 4; 597-612
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Gold nanoparticles in an enhancement of antimicrobial activity
Autorzy:
Wanarska, Ewelina
Maliszewska, Irena
Powiązania:
https://bibliotekanauki.pl/articles/1450221.pdf
Data publikacji:
2020
Wydawca:
Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
Tematy:
gold nanoparticles
photodynamic therapy
methylene blue
staphylococcus aureus
Opis:
The effect of antimicrobial photodynamic therapy (aPDT) on Gram-positive bacterium Staphylocccus aureus was studied. Methylene blue (MB) at non-toxic concentration of 31.25µg/ml was used as a photosensitizer. LEDs diodes were used as a light source to study the effect of methylene blue alone and the MB-gold nanoparticle mixture on the viability of S. aureus cells. Biogenic gold nanoparticles (biolAuNPs, 10ppm) and chemically synthesized gold nanoparticles (chemAuNPs, 3ppm) were tested as enhancement agents. In the presence of MB alone as a photosensitizer, the killing effect was about 92% after 30min of irradiation. The aPDT therapy was enhanced by addition of biolAuNPs and chemAuNPs and killing rate of S. aureus was 95-96% after 30min of irradiation. The probable mechanism of enhancement of MB-mediated photodynamic bactericidal efficacy against S. aureus in the presence of gold nanoparticles is discussed leading to the conclusion that colloidal gold increases the accumulation of MB in bacterial cells.
Źródło:
Physicochemical Problems of Mineral Processing; 2020, 56, 6; 269-279
1643-1049
2084-4735
Pojawia się w:
Physicochemical Problems of Mineral Processing
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Evaluation of P1 substrate specificity of staphylococcal SplB protease
Autorzy:
Pustelny, Katarzyna
Stach, Natalia
Wladyka, Benedykt
Dubin, Adam
Dubin, Grzegorz
Powiązania:
https://bibliotekanauki.pl/articles/1039355.pdf
Data publikacji:
2014
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
serine protease
serine protease-like
SplB
Staphylococcus aureus
substrate specificity
Opis:
Staphylococcus aureus is a dangerous human pathogen characterized by growing antibiotic resistance. Virulence of S. aureus relies on a variety of secreted and cell surface associated virulence factors among which certain proteolytic enzymes play an important role. Amid staphylococcal extracellular proteases, those encoded by the spl operon remain poorly characterized, both in terms of enzymology and their physiological role. Initial data demonstrated that Spl proteases exhibit restricted substrate specificity. This study describes development of convenient protein FRET substrates for SplB protease and characterization of the substrate preference of the protease at the P1' position. Kinetic data on hydrolysis of a panel of substrates substituted at the said position is provided.
Źródło:
Acta Biochimica Polonica; 2014, 61, 1; 149-152
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-15 z 42

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