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Tytuł:
Evaluation of RAPD markers for molecular identification of five bamboo genera from Indonesia
Autorzy:
Annisa, -
Hafzari, Rini
Setiawati, Tia
Irawan, Budi
Kusmoro, Joko
Powiązania:
https://bibliotekanauki.pl/articles/2041955.pdf
Data publikacji:
2019
Wydawca:
Instytut Badawczy Leśnictwa
Tematy:
bamboo
genetics diversity
RAPD
variation
Opis:
Conservation of bamboos for future exploitation as fuel, fibre and as an ingredient for cosmetics depends on knowledge of its natural genetic variation. The study of molecular genetic diversity in bamboos will provide important information for its conservation. This article reports on the genetic diversity in 25 species representing five genera of bamboos found in Indonesia using Random Amplified Polymorphic DNA (RAPD) molecular markers. Out of 40 primers, 24 primers produced 1107 total bands and 86.21% of polymorphic bands across the 25 species. Sixteen bands were uniquely found in one species only and their presence or absence helped to define nine bamboo species. RAPD band sizes ranged from 162 to 2247 base pairs. A dendrogram based on the similarity coefficient of Dice divided the bamboo species into three big clusters. In conclusion, RAPD can capture the diversity among five different bamboo genera and has a great potential to be used in the study of genetic diversity in Indonesian bamboos.
Źródło:
Folia Forestalia Polonica. Series A . Forestry; 2019, 61, 4; 255-266
0071-6677
Pojawia się w:
Folia Forestalia Polonica. Series A . Forestry
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Molecular Diagnostic of Streptococcus thermophilus
Diagnostyka molekularna Streptococcus thermophilus
Autorzy:
Vanatkova, Z.
Okenkova, E.
Bunkova, L.
Drab, V.
Hrabe, J.
Powiązania:
https://bibliotekanauki.pl/articles/388393.pdf
Data publikacji:
2009
Wydawca:
Towarzystwo Chemii i Inżynierii Ekologicznej
Tematy:
Streptococcus thermophilus
PCR
RAPD
SDS-PAGE
Opis:
Streptococcus thermophilus is one of the most important lactic acid bacteria in the dairy industry. Despite the wide use of Streptococcus thermophilus in the industry, data on the phenotypic and genetic strain variations within the species are still limited. Genetic techniques are very useful for molecular discrimination of complex mixtures of starter and probiotic cultures in research laboratories. Detection and identification of various lactic acid bacteria species with rapid methods is often important for quality control of dairy products. This work deals with characterization and differentiation of strains Streptococcus thermophilus by PCR, RAPD and SDS-PAGE techniques. Fifteen strains of Streptococcus thermophilus from Czech Collection of Dairy Microorganisms (CCDM) and a strain of Streptococcus thermophilus from Czech Collection of Microorganisms (CCM) were used. Particular strains were confirmed with primer set THI/THII by PCR method. Consequently, their identities were examined by RAPD and SDS-PAGE techniques. Whereas, primers OPP-7 and RAPD-4, RAPD were used. It can be claimed that mentioned methods are good means for identification and characterization of streptococci.
Streptococcus thermofilus jest jednym z najważniejszych przedstawicieli bakterii kwasu mlekowego. Pomimo powszechnego zastosowania tego gatunku w przemyśle mleczarskim nadal nieliczne są dane na temat jego zróżnicowania fenotypowego i genetycznego Streptococcus thermofilus. Szybka identyfikacja różnych gatunków bakterii kwasu mlekowego ma duże znaczenie dla kontroli jakości produktów mleczarskich. Niniejsza praca dotyczy charakterystyk i różnic występujących między różnymi liniami Streptococcus thermofilus. Badania zostały przeprowadzone przy użyciu technik PCR, RAPD i SDS-PAGE. Do badań użyto 15 linii Streptococcus thermofilus z Czeskiego Zbioru Mikroorganizmów Mleczarskich oraz jednej linii Streptococcus thermofilus z Czeskiego Zbioru Mikroorganizmów. Dokonano porównania primerów THI/THII między poszczególnymi liniami bakterii za pomocą techniki PCR. Następnie próbki były badane i identyfikowane przy użyciu technik RAPD i SDS-PAGE. Natomiast primery OPP-7 i RAPD-4 zbadano techniką RAPD. Badania wykazały, że zastosowane techniki mogą być skutecznie wykorzystywane do identyfikacji i charakterystyki bakterii z rodzaju Streptococcus.
Źródło:
Ecological Chemistry and Engineering. A; 2009, 16, 12; 1627-1635
1898-6188
2084-4530
Pojawia się w:
Ecological Chemistry and Engineering. A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Application of the RAPD technique to identify genetic diversity in cultivated forms of Capsicum annuum L.
Autorzy:
Niklas, A.
Olszewska, D.
Powiązania:
https://bibliotekanauki.pl/articles/2096389.pdf
Data publikacji:
2021
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
RAPD technique
genetic diversity
Capsicum annuum L.
Opis:
Background. The extensive use of pepper fruit creates a constant demand for new cultivars with specific agromorphological properties. The wide variety of breeding materials of this species means that methods based on morphological traits descriptions are not always sufficient to allow for their identification. Genetic homogeneity must be guaranteed to ensure repeatability of phenotypic traits. Most often, molecular analyses characterizing diversity at the DNA level are used for this purpose. Material and methods. The PCR-RAPD technique was used for molecular analysis of the generative offspring of three cultivars of pepper: “Anchi”, “Luba” and “Sono” and their forms of different fruit colour. The genetic distance between the tested genotypes was determined using the Nei and Li formula. Results. The reaction with 26 RAPD primers resulted in a total of 262 products and 5.2% of them were polymorphic bands. Eight of the used primers generated 12 polymorphic products that differentiated the tested genotypes. The “Anchi” cultivar was identified by the primers A07, K10, Q07 and AE10. Starter Q07 identified as well the “Luba” cultivar. Reactions carried out with primers B10 and RAD1 identified the “Sono” cultivar. In addition, primer A15 generated products that made it possible to distinguish yellow-fruit and red-fruit forms within the “Luba” and “Sono” cultivars. Conclusion. The analyses showed a low degree of genetic distance between C. annuum L. cultivars confirming the genetic homogeneity of the examined groups of plants and creating and opportunity for molecular identification of the genetic diversity within the “Luba” and “Sono” cultivars.
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2021, 102, 2; 171-177
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The relationship between RAPD marker-by-marker interactions and quantitative traits of caraway (Carum carvi L.)
Autorzy:
Bocianowski, J.
Seidler-Łożykowska, K.
Nowosad, K.
Kuczyńska, A.
Powiązania:
https://bibliotekanauki.pl/articles/12681131.pdf
Data publikacji:
2019
Wydawca:
Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
Tematy:
plant cultivation
caraway
Carum carvi
herbal plant
spice plant
quantitative trait
molecular marker
RAPD marker
RAPD-PCR reaction
Opis:
Application of molecular markers makes the selection process much more effective. Marker assisted selection is an important tool for plant breeders to increase the efficiency of a breeding process, especially for multigenic traits, highly influenced by the environment. Epistasis is the interaction between alleles from two or more loci determining the complex traits, and thus plays an important role in the development of quantitative traits of crops. In this paper, the relationships between RAPD marker-by-marker interactions and 22 quantitative traits of caraway (Carum carvi L.) were analyzed. Significant associations of 116 epistatic markers with at least one trait in 2004 as well as 112 in 2005 were found on the basis of multivariate regression analysis. The proportion of total phenotypic variances of individual trait explained by the marker-by-marker interactions ranged from 25.3% to 96.0%.
Źródło:
Acta Scientiarum Polonorum. Hortorum Cultus; 2019, 18, 3; 53-69
1644-0692
Pojawia się w:
Acta Scientiarum Polonorum. Hortorum Cultus
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Genetic variability and virulence of some Iranian Rhizoctonia solani isolates associated with stem canker and black scurf of potato (Solanum tuberosum L.)
Autorzy:
Esfahani, M.N.
Powiązania:
https://bibliotekanauki.pl/articles/2084743.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
genetic diversity
isolates
ISSR
RAPD
virulence variability
Opis:
Stem canker and black scurf of potato (Solanum tuberosum L.) caused by Rhizoctonia solani Kühn are important and epidemic diseases in potato-growing regions worldwide, including Iran. In this study, 120 isolates were retrieved from infected stem canker from six potato-growing regions in Iran (Isfahan, Ardebil, Fars, Hamedan, Kurdestan and Kerman). Out of these, 30 isolates were selected as representatives for genetic and virulence analysis. The isolates were analyzed by one sequence analyzes of the ITS-rDNA region, random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR), as well as virulence studies. Based on sequence analysis of the ITS-rDNA region, all 30 isolates were assigned to the anastomosis group (AG) and all were assigned to AG-3 PT. Cluster analysis using the unweighted pair group method with the arithmetic averages (UPGMA) method for both RAPD and ISSR markers revealed that they were divided into three main groups, with no correlation to geographical regions of the isolates. Pathogenicity tests showed that all isolates were pathogenic on potato cv. Agria; however, virulence variability was observed among the isolates. The grouping based on RAPD analysis and virulence variability was not correlated.
Źródło:
Journal of Plant Protection Research; 2020, 60, 1; 21-30
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Genetic diversity of Betula nana in Sweden and conservation implications for protection of relict Polish populations
Autorzy:
Dąbrowska, Grażyna B.
Dąbrowski, Henryk P.
Szyp-Borowska, Iwona
Powiązania:
https://bibliotekanauki.pl/articles/2041027.pdf
Data publikacji:
2021
Wydawca:
Instytut Badawczy Leśnictwa
Tematy:
dwarf birch
RAPD
population
relict species
genetic diversity
Opis:
The natural range of the dwarf birch (Betula nana L.) includes the boreal, subarctic and arctic regions of Europe, Asia and North America, where it is relatively common. In Poland, it is a relict species occurring in fragmented populations. Using the random amplification of polymorphic DNA (RAPD) technique, we investigated the genetic diversity of the four Swedish populations representing a part of the continuous range of dwarf birch. With the knowledge of the level of genetic diversity of a population from a continuous distribution, we can assess the genetic status of polish populations and answer the question if habitat fragmentation and a decrease in population size lead to a loss in genetic diversity. Knowledge of genetic diversity is important for species conservation, especially to predict their ability to respond to environmental pressures. We found that the populations Abisko, Malbo, Gällivare and Storlien, which are located at the edge of the natural range of B. nana and occupy different habitats, are genetically diverse to varying degrees. The northern populations from Abisko and Gällivare showed a lower level of genetic polymorphism than the population from Malbo, the southernmost site of dwarf birch in Sweden. The data presented indicate higher genetic diversity existed within populations, whereas genetic differentiation between populations was lower. The high level of genetic differentiation within B. nana populations that were analysed in the present study may be explained by a limited capacity for dispersal among populations via both pollen and seeds. We found that the level of genetic diversity in one of the Polish populations of B. nana is comparable to that in areas in Scandinavia where populations are large and continuous. Based on these studies, we conclude that the “Linje” population has sufficient genetic resources.
Źródło:
Folia Forestalia Polonica. Series A . Forestry; 2021, 63, 3; 225-231
0071-6677
Pojawia się w:
Folia Forestalia Polonica. Series A . Forestry
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Investigation of the correlation between virulence factors and genotypic profiles of Candida albicans isolated from turkeys
Autorzy:
Sokół, I.
Gaweł, A.
Bobrek, K.
Powiązania:
https://bibliotekanauki.pl/articles/2087778.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Candida albicans
turkeys
genotypic profile
RAPD
proteinase
haemolysin
phospholipase
Źródło:
Polish Journal of Veterinary Sciences; 2018, 21, 1; 29-33
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Differentiation by random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) of Candida albicans isolated from upper respiratory tract in patients with non-small cell lung cancer
Autorzy:
Biernasiuk, Anna
Korona-Głowniak, Izabela
Grzegorczyk, Agnieszka
Malm, Anna
Powiązania:
https://bibliotekanauki.pl/articles/1039202.pdf
Data publikacji:
2014
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
RAPD-PCR
Candida albicans
upper respiratory tract
genetic diversity
Opis:
Cancer patients are predisposed to fungal infections caused by Candida albicans, especially to oral or respiratory tract candidiasis. The aim of this study was to estimate genetic diversity by RAPD-PCR (random amplified polymorphic DNA-polymerase chain reaction) of C. albicans isolated from upper respiratory tract of 100 patients with non-small cell lung cancer. Among 52 strains, 34 genotypes were defined. 10 clusters comprising 28 (53.85%) isolates with similarity coefficient ≥ 80% were formed. The remaining 24 (46.15%) isolates represented individual genotypes. The RAPD-PCR technique revealed genomic variability within C. albicans isolated from upper respiratory tract of the cancer patients.
Źródło:
Acta Biochimica Polonica; 2014, 61, 4; 727-729
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Genetic diversity of F1 and F2 interspecific hybrids between dwarf birch (Betula nana L.) and Himalayan birch (B. utilis var. jacquemontii (Spach) Winkl. 'Doorenbos') using RAPD-PCR markers and ploidy analysis
Autorzy:
Czernicka, Małgorzata
Pławiak, Jarosław
Muras, Piotr
Powiązania:
https://bibliotekanauki.pl/articles/1039274.pdf
Data publikacji:
2014
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
Betula nana
Betula utilis 'Doorenbos'
interspecific hybrids
ploidy analysis
RAPD-PCR
Opis:
Crosses between Betula nana and B. utilis 'Doorenbos' were undertaken in order to obtain interspecific hybrids which could be characterized by wide spreading stems, strong branching habit, decorative clear white bark and an interesting shape of purple leaves. The research purpose was to examine genetic diversity of the 16 F1 and F2 putative progenies by using the RAPD-PCR method and the ploidy analysis. A total of 242 RAPD markers were scored with 24 primers and 220 (90.9%) polymorphic bands were found. In the NJ dendrogram, cluster I consisted of the female parent - B. nana and 12 hybrids and cluster II grouped the male parent - B. utilis 'Doorenbos' with 4 hybrids (F2/2, F1/8, F1/7 and F2/1). The 2-D scaling by PCoA was in agreement with the similarity index, i.e. two hybrids (F1/8, F2/2) grouped with the male parent while others with female parent. Classification of the hybrid plants by chromosome counting demonstrated that 13 hybrids were confirmed with accurate chromosome counts as being diploid (2n=2x=28) and 3 plants (F1/7, F1/8, F2/2) as triploid with 42 chromosomes.
Źródło:
Acta Biochimica Polonica; 2014, 61, 2; 195-199
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Diversity of Armillaria ostoyae in Scots pine plantations in Poland
Autorzy:
Szewczyk, W.
Kwasna, H.
Bocianowski, J.
Behnke-Borowczyk, J.
Ratajczak, A.
Swietlik, A.
Powiązania:
https://bibliotekanauki.pl/articles/41365.pdf
Data publikacji:
2014
Wydawca:
Polska Akademia Nauk. Instytut Dendrologii PAN
Tematy:
diversity
Armillaria ostoyae
Scotch pine
plantation
RAPD-PCR method
rhizomorph
Polska
Opis:
Incidence of Armillaria root disease and the population structure of associated Armillaria spp. were studied in 5-17-year-old Scots pine plantations in west-central Poland. Two infection centres (1.14– 9.30 ha) in each of three forest districts (Siemianice, Zielonka and Złotów) were intensively sampled. Root collars were examined for mycelial fans, decayed wood, and rhizomorphs. Twenty two isolates of Armillaria ostoyae collected from epiphytic rhizomorphs from 20 living and two dead trees in the six infection centres were identified with somatic incompatibility group. Only one somatic incompatibility group for A. ostoyae was found. Twenty one isolates produced rhizomorphs on oak-wood discs submerged in a sand-forest soil substrate. Isolates from Siemianice formed the smallest rhizomorph networks and those from Złotów the most abundant. There were 16 different genets among 22 isolates of A. ostoyae distinguished by RAPD analysis. Genetic similarity among genets was 25.6–97.5%. The large diversity in A. ostoyae suggests that sexual reproduction may occur in nature more often than expected.
Źródło:
Dendrobiology; 2014, 72
1641-1307
Pojawia się w:
Dendrobiology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Characteristics of selected molecular methods used in identification and assessment of genetic diversity of bacteria belonging to the genus Azotobacter
Autorzy:
Kozieł, Monika
Gałązka, Anna
Powiązania:
https://bibliotekanauki.pl/articles/2147979.pdf
Data publikacji:
2019-09-30
Wydawca:
Instytut Uprawy Nawożenia i Gleboznawstwa – Państwowy Instytut Badawczy
Tematy:
Azotobacter
ITS PCR
16S rRNA gen
PCR MP
RAPD
ARDRA
Opis:
Modern molecular techniques have greatly increased our knowledge concerning phylogenetic and functional diversity of microorganisms inhabiting the soil environment. Soil ecosys-tem is relatively complex with a high level of microbiologically diversity. The application of traditional culture-based techniques dose not reflect the total diversity of microbial community in-habiting in soil environment. On the other hand commonly used molecular methods allow for quick and accurate identification and evaluation of the genetic diversity of microorganisms in-habiting this environment. Free-living bacteria belonging to the genus Azotobacter commonly occurring in soil. Azotobacter spp. are the subject of many studies conducted both in Poland and in the world. The interest in these bacteria is largely related to their properties very useful for agriculture. Owing to their capability of fixing atmospheric nitrogen and making it available to plants and production of plant growth promotion and fungicidal substances, they are used in the production of soil bacterial inoculants. In addition, these bacteria are an excellent indicator of soil fertil-ity, which is why they are often used as test microorganisms in many studies. The paper presents an overview of molecular mi-crobiological techniques used to identify and evaluate the genetic diversity of Azotobacter spp. in studies conducted both in Poland and across the world. The ITS PCR, PCR-RFLP methods and 16S rRNA gene amplification are used to identify bacteria of the ge-nus Azotobacter, and PCR MP, RAPD PCR and ARDRA are used to assess the genetic diversity of these microorganisms.
Źródło:
Polish Journal of Agronomy; 2019, 38; 37-45
2081-2787
Pojawia się w:
Polish Journal of Agronomy
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
LaCl3 induces genomic DNA instability and increases DNA methylation levels in wheat roots
Autorzy:
Lei, X.
Ma, K.
Zhang, F.
Powiązania:
https://bibliotekanauki.pl/articles/2117694.pdf
Data publikacji:
2021
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
cell cycle
Ca 2+ -channel blocker
RAPD
CRED-RA
Triticum aestivum
Opis:
Accumulation of LaCl3 , a well-known Ca 2+ -channel blocker, can inhibit plant growth. However, the current understanding of its effects on gene expression is limited. In this paper, different concentrations of LaCl 3 (0, 0.5, 1.0, 1.5, 2.0 mM) were used to treat germinated wheat (Triticum aestivum L.) seeds for 24 h. The degree of root growth inhibition gradually increased with increasing LaCl 3 concentration. qRT-PCR analysis revealed that the expression of several key genes related to the cell cycle process, such as pcna, mcm2, rdr and cyclin B, were significantly down-regulated. Further analysis of genomic DNA instability using Random Amplified Polymorphic DNA (RAPD) and methylation levels by Coupled Restriction Enzyme Digestion-Random Amplification (CRED-RA) analysis indicated a significant increase in genomic DNA polymorphisms and methylation levels. The results of this study verified that the reasons why LaCl3 treatment can inhibit the growth of wheat roots are as follows: interference in the normal progression of the cell cycle, induction of genomic DNA instability and increase in DNA methylation levels.
Źródło:
Acta Biologica Cracoviensia. Series Botanica; 2021, 63, 1; 31-41
0001-5296
Pojawia się w:
Acta Biologica Cracoviensia. Series Botanica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Genetic diversity of natural psammophilous populations of Hypogymnia physodes (L.) Nyl. on Polish seacoast dunes
Autorzy:
Wieczorek, Anetta
Achrem, Magdalena
Truszkowska, Aleksandra
Łysko, Andrzej
Popiela, Agnieszka
Powiązania:
https://bibliotekanauki.pl/articles/952610.pdf
Data publikacji:
2017
Wydawca:
Polskie Towarzystwo Botaniczne
Tematy:
genetic polymorphism
interpopulation variability
randomly amplifed
polymorphic dna
rapd
hypogymnia physodes
sand dunes
Opis:
Hypogymnia physodes is a lichenized fungus of the family Parmeliaceae. The aim of this study was to compare the level of genetic diversity in eight psammophilous and three epiphytic populations of this species from the Baltic coast in Poland, based on randomly amplified polymorphic DNA (RAPD) markers. In the reactions with nine primers, 153 fragments were obtained, of which 133 were polymorphic. In one reaction, from 0 (for lich2 primer) to 55 (for C02 primer) amplicons were obtained. A Dice’s genetic similarity index matrix was constructed based on the results of RAPD marker polymorphism examination. The values of similarity indices ranged from 0.00 to 0.73. Results of this study confirm the separateness of all three epiphytic populations from those found on sand dunes (100% support, UPGMA/1000 trees).
Źródło:
Acta Mycologica; 2017, 52, 1
0001-625X
2353-074X
Pojawia się w:
Acta Mycologica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Genetic variation in mutants of chilli (Capsicum annuum) revealed by RAPD marker
Autorzy:
Mullainathan, L.
Sridevi, A.
Umavathi, S.
Sanjai Gandhi, E.
Powiązania:
https://bibliotekanauki.pl/articles/11592.pdf
Data publikacji:
2014
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:
genetic variation
mutant
chilli
Capsicum annuum
random amplified polymorphic DNA analysis
RAPD marker
spice
Opis:
The present study was under taken in order to analyze the chemical mutagenesis on Chilli germplasm. In this regard, K1 variety of chilli was subjected to different mutagenic concentration for inducing mutagenesis. The M3 plants exposed to EMS and DES to produce clear difference from the untreated control, thus indicating that mutagenic treatment produce polymorphic regions in the chilli. For extraction of genomic DNA was adopted an improved protocol of CTAB method with slight modification. A total of ten primers were used to screen the polymorphism among the treated populations line tall, tall with chlorophyll deficient, leaf, flower, GMS and DNA damages in maturity mutants were analyzed with control. Out of ten primers, four primers (PGF02, PGF03, PGF04 AND OP107) were successfully amplified in all the samples used for this study. The successful primers were amplified in to 93 products showing an average of 9.3 bands.
Źródło:
International Letters of Natural Sciences; 2014, 06
2300-9675
Pojawia się w:
International Letters of Natural Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Identification of genetic diversity among Arnica montana L. genotypes using RAPD markers
Analiza zróżnicowania genetycznego wśród genotypów Arnica montana L. za pomocą markerów RAPD
Autorzy:
Okoń, S.
Paczos-Grzęda, E.
Łoboda, M.
Sugier, D.
Powiązania:
https://bibliotekanauki.pl/articles/11542962.pdf
Data publikacji:
2014
Wydawca:
Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
Tematy:
DNA polymorphism
identification
genetic diversity
Arnica montana
genotype
RAPD marker
medicinal plant
molecular analysis
Opis:
Arnica montana L. is one of the most important herbal plants used in medicine, pharmaceutical and cosmetic industry. The number of studies performed with molecular markers on arnica genotypes is very limited. Because of this fact the aims of presented examination were optimization of protocols DNA isolation from fresh leaves of A. montana and identification of genetic diversity among this plant genotypes. In presented study to obtain pure DNA Plant & Fungi DNA Purification Kit (EURx) were used. To clean obtained DNA long and slow electrophoresis and isolation DNA from gels were used. A. montana genotypes were analyzed using 40 RAPD primers (Operon Technologies), out of which 12 produced high number of polymorphic and repeatable fragments. In total, selected primers produced 120 fragments, among them 111 (92.5%) were polymorphic. The genetic similarity matrices were produced based on RAPD using the Dice’s coefficient. RAPD based genetic similarity was estimated between 0.535 and 0.945. The highest genetic similarity was estimated among GA17 and GA18 genotypes, which are closely located on the obtained dendrogramme.
Arnica montana L. jest jedną z najcenniejszych roślin zielarskich wykorzystywanych w medycynie, farmacji i przemyśle kosmetycznym. W dostępnej literaturze liczba doniesień związanych z analizą molekularną arniki jest znikoma, dlatego też celem prezentowanych badań była optymalizacja procesu izolacji DNA ze świeżych liści oraz identyfikacja zróżnicowania genetycznego oparta na markerach RAPD. W prezentowanej pracy w celu uzyskania czystego DNA do izolacji wykorzystano zestaw DNA Plant & Fungi DNA Purification Kit (Euro) oraz oczyszczanie za pomocą długiej elektroforezy w żelu agarozowym. Spośród testowanych 40 starterów RPAD do analiz wybrano 12 generujących stabilne i polimorficzne wzory prążków. Wyselekcjonowane startery amplifikowały 120 fragmentów, spośród których 111 (92,5%) było polimorficznych. Wykorzystujac markery RAPD utworzono matryce podobieństwa genetycznego. średnia wartość podobieństwa analizowanych genotypów wynosiła 0.886. Najwyższy współczynnik podobieństwa genetycznego oszacowano pomiędzy genotypami GA17 i GA18, które ulokowały się blisko siebie na uzyskanym dendrogramie.
Źródło:
Acta Scientiarum Polonorum. Hortorum Cultus; 2014, 13, 4; 63-71
1644-0692
Pojawia się w:
Acta Scientiarum Polonorum. Hortorum Cultus
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-15 z 21

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