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    Wyświetlanie 1-5 z 5
    Tytuł:
    Simulation of life raft motions on irregular wave - an analysis of situations leading to raft capsizing
    Autorzy:
    Kazimierska, O.
    Powiązania:
    https://bibliotekanauki.pl/articles/260566.pdf
    Data publikacji:
    2018
    Wydawca:
    Politechnika Gdańska. Wydział Inżynierii Mechanicznej i Okrętownictwa
    Tematy:
    life rafts
    inflatable rafts
    circular rafts
    drift anchors
    simulations of motions on irregular wave
    raft dynamics
    wave spectrum
    Opis:
    Successful rescue action at sea is based on a. o. a correct choice of rescue means and their reliability. Operational characteristics of life-saving appliances determine their performance in a given water area. Therefore they affect duration time of rescue action and decide this way on survival time of shipwrecked persons. This paper presents impact of characteristics of circular inflatable life rafts on their dynamics in a given sea environment. Particular issues dealing with raft motion were analyzed and their solutions were then presented in the form of computer programs built into MATLAB environment. The programs operate on the open choice basis– the user is requested to introduce data of a raft to be investigated. The program clearly informs which units should be used for the data. The obtained results allow to limit survivours searching area and simultaneously form the basis for working out a model of searched object drift motion. In this work the use was made of the results obtained from the KBN research project No. 4T12C03827 in which an attempt was made to analyzing capsize probability of a raft at given sea states. The raft investigated in the project is also used as a reference raft in the computer programs developed in this work.
    Źródło:
    Polish Maritime Research; 2018, S 1; 42-50
    1233-2585
    Pojawia się w:
    Polish Maritime Research
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Smooth muscle actomyosin promotes Ca2+-dependent interactions between annexin VI and detergent-insoluble glycosphingolipid-enriched membrane domains.
    Autorzy:
    Babiychuk, Victoria
    Draeger, Annette
    Babiychuk, Eduard
    Powiązania:
    https://bibliotekanauki.pl/articles/1044291.pdf
    Data publikacji:
    2000
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    annexins
    calcium
    rafts
    smooth muscle
    cell membrane
    Opis:
    The mechanical link coupling cytoskeletal and contractile proteins to the sarcolemma of smooth muscle cells is essential for transmitting tension from the cell's interior to exterior. In addition to the well-characterized actin-integrin associations present in adhaerens junctions, our recent work has postulated the existence of a reversible annexin-dependent membrane-cytoskeleton complex, forged in response to a rise in intracellular Ca2+ concentration following smooth muscle cell stimulation (Babiychuk et al., J. Biol Chem. 1999, 274, 35191-35195). Detailed biochemical characterization of the interactions responsible for the formation of this complex revealed that annexins II and VI interact with actomyosin, or detergent-insoluble glycosphingolipid-enriched membrane domains (rafts) purified from smooth muscle, in a concentration- and Ca2+-dependent manner. Annexin II interacted with lipid rafts with high Ca2+-sensitivity, while for annexin VI this interaction required non-physiologically high concentrations of free Ca2+. However, the Ca2+-sensitivity of the latter interaction strongly increased in the presence of purified smooth muscle actomyosin. The detailed biochemical analysis of the interactions occurring between annexin II, annexin VI, actomyosin and rafts suggests that annexins regulate sarcolemmal organization during smooth muscle cell contraction.
    Źródło:
    Acta Biochimica Polonica; 2000, 47, 3; 579-589
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Cardiovascular testing of seasickness in healthy volunteers on life rafts
    Autorzy:
    Molisz, Andrzej
    Ryk, Małgorzata
    Rataj, Michał
    Kołaczyńska, Barbara
    Nowicka-Sauer, Katarzyna
    Przewoźny, Tomasz
    Sauer, Marta
    Gutknecht, Piotr
    Trzeciak, Bartosz
    Siebert, Janusz
    Powiązania:
    https://bibliotekanauki.pl/articles/2116572.pdf
    Data publikacji:
    2020-06-16
    Wydawca:
    Instytut Medycyny Pracy im. prof. dra Jerzego Nofera w Łodzi
    Tematy:
    survival
    impedance cardiography
    seasickness
    life rafts
    MSAQ
    MSSQ
    Opis:
    ObjectivesThe role of the cardiovascular system in the development of seasickness remains uncertain.Material and MethodsOverall, 18 healthy students (10 males and 8 females) aged 18–24 years volunteered in the project, spending 2–7 h on life rafts. The cardiovascular system was examined with impedance cardiography. Susceptibility and symptoms of seasickness were evaluated by the Motion Sickness Susceptibility Questionnaire Shortform (MSSQ-Short) and the Motion Sickness Assessment Questionnaire (MSAQ). The Visual Analogue Scale (VAS), ranging 0–10, was used to assess nausea, dizziness and mood. The parameters were assessed at 2 time points.ResultsDifferences in the heart rate (HR), the thoracic fluid content index (TFCI), the stroke index (SI) and the Heather index (HI) before launching the life rafts and after leaving them were observed (78.6, 20.8, 55.6 and 15.9 vs. 70.1, 19.7, 60.5 and 17.9, with p-values of 0.002, <0.001, 0.003 and 0.004, respectively). Females reacted with changes in SI and HR more vividly, whereas males regulated more HI and TFCI. In addition, HR correlated significantly with the central and peripheral symptoms in MSAQ, stroke volume (SV) with peripheral and sopite-related ones, SI with overall ones, and pulse pressure with overall, gastrointestinal and central ones (Spearman’s rank correlation coefficient [ρ] was –0.478, –0.711, 0.476, 0.472, 0.525, –0.476, –0.579 and –0.584, respectively). As regards MSSQ-Short, it correlated negatively with sopite-related symptoms in MSAQ (ρ= –0.486). Mood in VAS correlated significantly with gastrointestinal symptoms, SI and the cardiac index (CI) (ρ = –0.752, –0.492 and –0.489, respectively).ConclusionsIt was found that HR correlated negatively, and SV/SI correlated positively, with the severity of seasickness symptoms measured with MSAQ. Gender is probably an independent factor influencing reactions to motion. Women react with SI increase whereas men react with increased heart contractility (HI rise). Negative mood in seasickness evaluated with VAS seems to be mostly determined by gastrointestinal symptoms assessed with MSAQ and diminished cardiovascular indices (both CI and SI).
    Źródło:
    International Journal of Occupational Medicine and Environmental Health; 2020, 33, 4; 467-477
    1232-1087
    1896-494X
    Pojawia się w:
    International Journal of Occupational Medicine and Environmental Health
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Giant unilamellar vesicles - a perfect tool to visualize phase separation and lipid rafts in model systems
    Autorzy:
    Wesołowska, Olga
    Michalak, Krystyna
    Maniewska, Jadwiga
    Hendrich, Andrzej
    Powiązania:
    https://bibliotekanauki.pl/articles/1040608.pdf
    Data publikacji:
    2009
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    phase separation
    lipid rafts
    model membranes
    giant unilamellar vesicles
    Opis:
    Model systems such as black lipid membranes or conventional uni- or multilamellar liposomes are commonly used to study membrane properties and structure. However, the construction and dimensions of these models excluded their direct optical microscopic observation. Since the introduction of the simple method of liposome electroformation in alternating electric field giant unilamellar vesicles (GUVs) have become an important model imitating biological membranes. Due to the average diameter of GUVs reaching up to 100 µm, they can be easily observed under a fluorescent or confocal microscope provided that the appropriate fluorescent probe was incorporated into the lipid phase during vesicle formation. GUVs can be formed from different lipid mixtures and they are stable in a wide range of physical conditions such as pH, pressure or temperature. This mini-review presents information about the methods of GUV production and their usage. Particularly, the use of GUVs in studying lipid phase separation and the appearance and behavior of lipid domains (rafts) in membranes is discussed but also other examples of GUVs use in membrane research are given. The experience of the authors in setting up the GUV-forming equipment and production of GUVs is also presented.
    Źródło:
    Acta Biochimica Polonica; 2009, 56, 1; 33-39
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Plasma membrane rafts and chaperones in cytokine/ STAT signaling.
    Autorzy:
    Sehgal, Pravin
    Powiązania:
    https://bibliotekanauki.pl/articles/1043429.pdf
    Data publikacji:
    2003
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    cytokine receptors
    chaperones
    fever
    heat shock protein 90 (HSP90)
    signal transducers and activators of transciption (STATs)
    caveolin
    lipid rafts
    Opis:
    We and others have recently obtained data suggesting that cytokine-STAT signaling in many different cell-types is a chaperoned pathway initiated at the level of specialized plasma membrane microdomains called "rafts" (the "raft-STAT signaling hypothesis"). These findings are of broad significance in that all cytokines and growth factors initiate signaling in target cells by interacting with respective cell-surface receptors. The new data suggest that raft microdomains represent the units of function at the cell-surface through which ligand-stimulated STAT signaling is initiated. Moreover, recent evidence shows the involvement of chaperone proteins in regulating the STAT signaling pathway. These chaperones include the human homolog of the tumorous imaginal disc 1 protein (hTid1) which associates with Janus kinase 2 (JAK2) at the level of the plasma membrane, heat shock protein 90 (HSP90) which associates with STAT3 and STAT1 proteins in caveolin-1-containing raft and cytoplasmic complexes, and glucose regulated protein 58 (GRP58/ER-60/ERp57), a thiol dependent protein-disulfide isomerase, found in association with STAT3 "statosome" complexes in the cytosol and in the raft fraction. We suggest a function of the HSP90 chaperone system in preserving IL-6/STAT3 signaling in liver cells in the context of fever. The identification and function of protein partners associated with specific STAT species in rafts and in cytosolic complexes, and in the efficient departure of cytokine-activated STATs from the cytosolic face of rafts towards the cell nucleus are now areas of active investigation.
    Źródło:
    Acta Biochimica Polonica; 2003, 50, 3; 583-594
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-5 z 5

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