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    Wyświetlanie 1-13 z 13
    Tytuł:
    Allelic polymorphism of endothelial NO-synthase gene and its functional manifestations
    Autorzy:
    Dosenko, Victor
    Zagoriy, Vyacheslav
    Haytovich, Nikolay
    Gordok, Olga
    Moibenko, Alexey
    Powiązania:
    https://bibliotekanauki.pl/articles/1041240.pdf
    Data publikacji:
    2006
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    activity of nitric oxide synthase
    endothelial nitric oxide synthase
    RNA expression
    platelets
    allelic polymorphism
    Opis:
    Investigation of the mechanisms of phenotypic realization of allelic polymorphism of the eNOS gene has shown that the level of eNOS mRNA and activity of this enzyme in platelets depends from genotype. We identified a T-786→C polymorphism in the promoter region, a variable number of tandem repeats (4a/4b) in intron 4 and the G894→T polymorphism in exon 7 of the eNOS gene in isolated human platelets. We measured eNOS mRNA in isolated platelets by reverse transcription-PCR and eNOS enzyme activity by fluorimetric detection system FCANOS-1 using diaminofluorescein diacetate (DAF-2A). It was shown that the level of eNOS mRNA is the lowest for the -786C/C promoter genotype. In exon 7 homozygotes (894T/T) the level of RNA is lower than in normal homozygotes (894G/G), but higher than in heterozygotes (894G/T). The eNOS activity in platelets is lower in carriers of the 786C/C promoter genotype than in normal homozygotes (2.1 × P=0.03), and lower comparing to heterozygotes (2.9 × P>0.05). The eNOS activity accompanying the 894T/T variant of exon 7 is also lower than in normal homozygotes (P>0.05). Regarding the polymorphism in intron 4 - the enzyme's activity is lower in carriers of the 4a/4a genotype comparing to normal homozygotes (1.7 × P>0.05) and lower than in heterozygotes (1.9 × P>0.05). These results allow one to conclude that the T-786→C polymorphism of the eNOS gene promoter most significantly affects the gene expression and eNOS activity.
    Źródło:
    Acta Biochimica Polonica; 2006, 53, 2; 299-302
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Polymorphism in intron 23 of the endothelial nitric oxide synthase gene (NOS3) is not associated with hypertension.
    Autorzy:
    Derebecka, Natalia
    Hołysz, Marcin
    Dankowski, Rafał
    Wierzchowski, Michał
    Trzeciak, Wiesław
    Powiązania:
    https://bibliotekanauki.pl/articles/1043838.pdf
    Data publikacji:
    2002
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    nitric oxide synthase
    gene polymorphism
    hypertension
    intron 23
    Opis:
    Nitric oxide (NO) is synthesised in the vascular endothelium by nitric oxide synthase (NOS3) and is an important factor in the regulation of blood pressure. Impaired synthesis of NO due to mutations in the NOS3 gene is associated with hypertension. To date several allelic variants of the NOS3 gene have been identified and their possible linkage with hypertension investigated. We studied the distribution of genotypes and frequency of alleles of the G11T polymorphism in intron 23 of the NOS3 gene in patients with hypertension and in a control group of healthy individuals. The polymorphism was determined by PCR-RFLP analysis. The distribution of genotypes in the patients with hypertension and in the healthy individuals did not differ significantly from the values predicted from Hardy-Weinberg equilibrium for the general population. No major differences in the distribution of the G11T polymorphism in the patients and healthy individuals were found (P > 0.05).
    Źródło:
    Acta Biochimica Polonica; 2002, 49, 1; 263-268
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Histochemical study of the nitric oxide synthase activity in experimental trichinellosis
    Autorzy:
    Hadas, E.
    Gustowska, L.
    Boczon, K.
    Janczewska, D.
    Powiązania:
    https://bibliotekanauki.pl/articles/837102.pdf
    Data publikacji:
    1998
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    parasite
    nitric oxide synthase
    mouse
    mice
    trichinellosis
    enzyme
    nitric oxide
    Trichinella spiralis
    synthase activity
    Źródło:
    Annals of Parasitology; 1998, 44, 3
    0043-5163
    Pojawia się w:
    Annals of Parasitology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Effect of oligochitosans on expression of inos gene in ehrlich ascites tumour in vitro
    Autorzy:
    Ignacak, Jan
    Wiśniewska-Wrona, Maria
    Dulińska-Litewka, Joanna
    Pałka, Iwona
    Zagajewski, Jacek
    Niekraszewicz, Antoni
    Powiązania:
    https://bibliotekanauki.pl/articles/1035407.pdf
    Data publikacji:
    2012
    Wydawca:
    Sieć Badawcza Łukasiewicz - Polskie Towarzystwo Chitynowe
    Tematy:
    chitosan oligomers
    histone H1
    iNOS (inducible nitric oxide synthase)
    nitric oxide
    Opis:
    Oligochitosans obtained through degradation of macromolecules of chitosan with a high degree of deacetylation turned out to be biologically active, contributing to an increase of nitric oxide levels in Ehrlich ascites tumor (EAT) cells through inducing expression of the isoform of inducible nitric oxide synthase (iNOS) gene. An increase of NO levels in EAT cells in the presence of the investigated oligochitosans might contribute to nitrosylation of L-cysteine – an allosteric effector of the M2 isoenzyme of pyruvate kinase (PK), which switches the PK kinase activity, responsible for ATP synthesis, to the histone kinase activity that may participate in histone H1 phosphorylation. Lack of the histone activity of the PK M2 isoenzyme may contribute to decreased histone H1 phosphorylation and thus inhibit EAT cells proliferation.
    Źródło:
    Progress on Chemistry and Application of Chitin and its Derivatives; 2012, 17; 141-148
    1896-5644
    Pojawia się w:
    Progress on Chemistry and Application of Chitin and its Derivatives
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Nonsteroidal anti-inflammatory drugs impair oral mucosal repair by eliciting disturbances in endothelin-converting enzyme-1 and constitutive nitric oxide synthase
    Autorzy:
    Slomiany, B.L.
    Slomiany, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/69594.pdf
    Data publikacji:
    2001
    Wydawca:
    Polskie Towarzystwo Fizjologiczne
    Tematy:
    nonsteroidal antiinflammatory drug
    nitric oxide synthase
    ulcer healing
    oral mucosa
    indomethacin
    impairment
    aspirin
    apoptosis
    Źródło:
    Journal of Physiology and Pharmacology; 2001, 52, 1
    0867-5910
    Pojawia się w:
    Journal of Physiology and Pharmacology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Influence of elastin-derived peptides, glucose, LDL and oxLDL on nitric oxide synthase expression in human umbilical artery endothelial cells
    Autorzy:
    Garczorz, Wojciech
    Francuz, Tomasz
    Gmiński, Jan
    Likus, Wirginia
    Siemianowicz, Krzysztof
    Jurczak, Teresa
    Strzałka-Mrozik, Barbara
    Powiązania:
    https://bibliotekanauki.pl/articles/1039891.pdf
    Data publikacji:
    2011
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    nitric oxide synthase
    oxidized LDL
    LDL
    EDP
    atherosclerosis
    hyperglycemia
    endothelium
    elastin-derived peptides
    eNOS
    Opis:
    Endothelial dysfunction plays an important role in the development of atherosclerosis. Elastin-derived peptides (EDP), hyperglycemia, hypercholesterolemia and oxidized LDL have a proven proatherosclerotic potential. Nitric oxide generated by endothelial nitric oxide synthase (eNOS; EC 1.14.13.39) is an important vasorelaxant. Here we studied the influence of those proatherosclerotic factors on eNOS gene and protein expression in artery-derived endothelial cells. Human umbilical artery endothelial cells (HUAEC) were incubated with or without: glucose (270 mg/dl), LDL (200 mg/dl), oxidized LDL (oxLDL 25 mg/dl) or κ-elastin (0.5 and 2.5 µg/ml). Gene expression was assessed by real time RT-PCR, whilst the eNOS protein by ELISA. In cells incubated with 2.5 µg/ml of κ-elastin, a 31 % increase of eNOS mRNA expression was observed, but the protein level remained unchanged. OxLDL, LDL and glucose decreased the eNOS protein level by 74 %, 37 % and 29 %, respectively. OxLDL decreased eNOS mRNA by 42 %. LDL non-significantly decreased eNOS mRNA expression. An elevated glucose level did not affect the eNOS mRNA expression. Hyperglycemia and an elevated level of LDL, particularly oxLDL, decreased the level of eNOS protein in endothelial cells. As κ-elastin did not decrease the expression of eNOS gene in HUAEC, the proatherogenic properties of elastin-derived peptides are unlikely to be due to their influence on eNOS.
    Źródło:
    Acta Biochimica Polonica; 2011, 58, 3; 375-379
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Single-electron reduction of quinone and nitroaromatic xenobiotics by recombinant rat neuronal nitric oxide synthase
    Autorzy:
    Anusevičius, Žilvinas
    Nivinskas, Henrikas
    Šarlauskas, Jonas
    Sari, Marie-Agnes
    Boucher, Jean-Luc
    Čėnas, Narimantas
    Powiązania:
    https://bibliotekanauki.pl/articles/1039577.pdf
    Data publikacji:
    2013
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    antitumour agents
    nitroaromatic compounds
    electron transfer mechanism
    oxidative stress
    quinones
    Neuronal nitric oxide synthase (nNOS)
    Opis:
    We examined the kinetics of single-electron reduction of a large number of structurally diverse quinones and nitroaromatic compounds, including a number of antitumour and antiparasitic drugs, and nitroaromatic explosives by recombinant rat neuronal nitric oxide synthase (nNOS, EC 1.14.13.39), aiming to characterize the role of nNOS in the oxidative stress-type cytotoxicity of the above compounds. The steady-state second-order rate constants (kcat/Km) of reduction of the quinones and nitroaromatics varied from 102 M-1s-1 to 106 M-1s-1, and increased with an increase in their single-electron reduction potentials (E17). The presence of Ca2+/calmodulin enhanced the reactivity of nNOS. These reactions were consistent with an 'outer sphere' electron-transfer mechanism, considering the FMNH./FMNH2 couple of nNOS as the most reactive reduced enzyme form. An analysis of the reactions of nNOS within the 'outer sphere' electron-transfer mechanism gave the approximate values of the distance of electron transfer, 0.39-0.47 nm, which are consistent with the crystal structure of the reductase domain of nNOS. On the other hand, at low oxygen concentrations ([O2] = 40-50 μM), nNOS performs a net two-electron reduction of quinones and nitroaromatics. This implies that NOS may in part be responsible for the bioreductive alkylation by two-electron reduced forms of antitumour aziridinyl-substituted quinones under a modest hypoxia.
    Źródło:
    Acta Biochimica Polonica; 2013, 60, 2; 217-222
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Amyloid beta enhances cytosolic phospholipase A2 level and arachidonic acid release via nitric oxide in APP-transfected PC12 cells
    Autorzy:
    Chalimoniuk, Małgorzata
    Stolecka, Anna
    Cąkała, Magdalena
    Hauptmann, Susane
    Schulz, Kris
    Lipka, Uta
    Leuner, Kristine
    Eckert, Anne
    Muller, Walter
    Strosznajder, Joanna
    Powiązania:
    https://bibliotekanauki.pl/articles/1041051.pdf
    Data publikacji:
    2007
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    nitric oxide synthase
    amyloid beta
    calcium
    arachidonic acid
    antioxidative enzymes
    reactive oxygen species
    cytosolic phospholipase A_2
    mitochondria
    Opis:
    Cytosolic phospholipase A2 (cPLA2) preferentially liberates arachidonic acid (AA), which is known to be elevated in Alzheimer's disease (AD). The aim of this study was to investigate the possible relationship between enhanced nitric oxide (NO) generation observed in AD and cPLA2 protein level, phosphorylation, and AA release in rat pheochromocytoma cell lines (PC12) differing in amyloid beta secretion. PC12 control cells, PC12 cells bearing the Swedish double mutation in amyloid beta precursor protein (APPsw), and PC12 cells transfected with human APP (APPwt) were used. The transfected APPwt and APPsw PC12 cells showed an about 2.8- and 4.8-fold increase of amyloid β (Aβ) secretion comparing to control PC12 cells. An increase of NO synthase activity, cGMP and free radical levels in APPsw and APPwt PC12 cells was observed. cPLA2 protein level was higher in APPsw and APPwt PC12 cells comparing to PC12 cells. Moreover, phosphorylated cPLA2 protein level and [3H]AA release were also higher in APP-transfected PC12 cells than in the control PC12 cells. An NO donor, sodium nitroprusside, stimulated [3H]AA release from prelabeled cells. The highest NO-induced AA release was observed in control PC12 cells, the effect in the other cell lines being statistically insignificant. Inhibition of cPLA2 by AACOCF3 significantly decreased the AA release. Inhibitors of nNOS and γ-secretase reduced AA release in APPsw and APPwt PC12 cells. The basal cytosolic [Ca2+]i and mitochondrial Ca2+ concentration was not changed in all investigated cell lines. Stimulation with thapsigargin increased the cytosolic and mitochondrial Ca2+ level, activated NOS and stimulated AA release in APP-transfected PC12 cells. These results indicate that Aβ peptides enhance the protein level and phosphorylation of cPLA2 and AA release by the NO signaling pathway.
    Źródło:
    Acta Biochimica Polonica; 2007, 54, 3; 611-623
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Relation between expression of TNF alpha, iNOS, VEGF mRNA and development of heart failure after experimental myocardial infarction in rats
    Autorzy:
    Heba, G.
    Krzeminski, T.
    Porc, M.
    Grzyb, J.
    Dembinska-Kiec, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/69900.pdf
    Data publikacji:
    2001
    Wydawca:
    Polskie Towarzystwo Fizjologiczne
    Tematy:
    nitric oxide synthase
    tumour necrosis factor-alpha
    cytokine
    ischaemic myocardium
    mRNA
    thoracotomy
    growth factor
    rat
    myocardial infarction
    vascular endothelial growth factor
    Źródło:
    Journal of Physiology and Pharmacology; 2001, 52, 1
    0867-5910
    Pojawia się w:
    Journal of Physiology and Pharmacology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    UV-B induced oxidative stress and protective effects of NO under myo-inositol-deficient background in Arabidopsis
    Autorzy:
    Lytvyn, D.
    Bergounioux, C.
    Yemets, A.
    Blume, Y.
    Powiązania:
    https://bibliotekanauki.pl/articles/80154.pdf
    Data publikacji:
    2013
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    conference
    ultraviolet-B radiation
    oxidative stress
    protective effect
    nitric oxide
    plant cell
    myo-inositol-1-phosphate synthase
    Źródło:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 2
    0860-7796
    Pojawia się w:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Age-related alterations in arginase-NO-synthase system in patients with coronary heart disease associated with hypertension
    Związane z wiekiem zmiany w układzie arginazy-NO-syntazy w grupie pacjentów z chorobą wieńcową współistniejącą z nadciśnieniem tętniczym
    Autorzy:
    Besedina, Anna
    Powiązania:
    https://bibliotekanauki.pl/articles/1036715.pdf
    Data publikacji:
    2016
    Wydawca:
    Śląski Uniwersytet Medyczny w Katowicach
    Tematy:
    coronary heart disease
    hypertension
    nitric oxide
    no-synthase
    arginase
    choroba wieńcowa
    nadciśnienie tętnicze
    tlenek azotu
    no-syntaza
    arginaza
    Opis:
    INTRODUCTION: Coronary heart disease (CHD) is the most common heart diseases in Europe. The aim of this study was to determine the intensity of L-arginine metabolism by two alternative pathways (oxidative by NOS and nonoxid-ative by arginase) in the blood plasma of patients with CHD associated with hypertension (HT) of different age groups. MATERIALS AND METHODS: 50 patients with isolated CHD and 42 patients with CHD associated with HT were enrolled in this study. NOS activity was determined by nitrite anion formed in the reaction. Arginase activity was tested by the formation of urea. RESULTS: In middle-aged patients with isolated CHD, the total NOS activity statistically significantly increased by 2.2 fold in comparison with healthy subjects of the same age group. In patients with CHD associated with HT, the total NOS activity statistically significantly increased in both middle-aged and older persons 2.3-fold than in healthy subjects of the same age groups. In patients with isolated CHD, the arginase activity increases 1.5-fold in middle-aged patients and 1.7-fold in older patients compared with the healthy participants. In the middle-aged and older patients with CHD associated with HT, the arginase activity statistically significantly increased 1.7- and 1.8-fold than in the healthy subjects of the same age groups. CONCLUSIONS: In patients with isolated CHD and patients with CHD associated with HT, an increased total NO-synthase and arginase activity in comparison with healthy individuals was found. It was shown that the increase in NO-synthase and arginase activity is more expressed in older patients than middle-aged patients.
    WSTĘP: Choroba wieńcowa jest najpowszechniejszą z chorób serca występujących na terenie Europy. Celem pracy było zbadanie intensywności metabolizmu L-argininy dwoma alternatywnymi sposobami (utleniającym przez NOS i nieutleniającym poprzez arginazę) w osoczu krwi pacjentów cierpiących na chorobę wieńcową (ChW) serca, współistniejącą z nadciśnieniem tętniczym (NT), w grupie pacjentów w różnym wieku. MATERIAŁ I METODY: Do badania włączono 50 pacjentów z izolowaną postacią ChW i 42 z ChW współistniejącą z NT. Aktywność NOS została wyznaczona przez anion azotynowy utworzony w reakcji. Aktywność arginazy sprawdzono przez formowanie mocznika. WYNIKI: Pacjenci w średnim wieku, z izolowaną postacią ChW wykazywali 2,2 razy wyższą całkowitą aktywność NOS w porównaniu ze zdrowymi badanymi w tej samej grupie wiekowej. W przypadku pacjentów z ChW współistniejącą z NT całkowita aktywność NOS była znacząco (2,3 razy) zwiększona w obu grupach wiekowych (w średnim oraz starszym wieku) w porównaniu z osobami zdrowymi w tym samym wieku. U pacjentów z izolowaną postacią ChW aktywność arginazy była 1,5 razy podwyższona u osób w średnim wieku oraz 1,7 razy u starszych niż u osób zdrowych. W wieku średnim i starszym pacjenci ze współistniejącą postacią ChW wraz z NT wykazywali statystycznie znacznie podwyższoną aktywność arginazy – odpowiednio 1,7 oraz 1,8 razy większą niż u zdrowych w tym samym wieku. WNIOSKI: Wykazano, że w grupie pacjentów z izolowaną postacią ChW oraz w przypadku współistnienienia ChW i NT aktywność NO-syntazy i arginazy jest wyższa niż u osób zdrowych. Zwiększona aktywność NO-syntazy i arginazy jest bardziej znacząca u pacjentów starszych.
    Źródło:
    Annales Academiae Medicae Silesiensis; 2016, 70; 40-45
    1734-025X
    Pojawia się w:
    Annales Academiae Medicae Silesiensis
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Histochemical study of the nitric oxide synthase activity in experimental trichinellosis
    Autorzy:
    Hadaś, E.
    Gustowska, L.
    Boczoń, K.
    Janczewska, D.
    Powiązania:
    https://bibliotekanauki.pl/articles/2148736.pdf
    Data publikacji:
    1999
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    toxic property
    trichinellosis
    nitric oxide
    morphological transformation
    parasitology
    muscle cell
    signalling property
    infectious disease
    physiological function
    synthase activity
    histochemistry
    Opis:
    Nitric oxide plays a critical role in a variety of biological acfivities. It has been nicknamed a ,,killer'' and ,,mediator" due to its toxic and signalling properties. Apart from its regular physiological function, nitric oxide indirectly participates in infectious diseases. Our report seems to be first presentation of the nitric oxide synthase participation in the host biochemical defence mechanisms and in morphological transformation of muscle cells in trichinellosis.
    Źródło:
    Wiadomości Parazytologiczne; 1999, 45, 1; 63-68
    0043-5163
    Pojawia się w:
    Wiadomości Parazytologiczne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Histochemical study of the nitric oxide synthase activity in experimental trichinellosis
    Autorzy:
    Hadas, E
    Gustowska, L.
    Boczon, K.
    Janczewska, D.
    Powiązania:
    https://bibliotekanauki.pl/articles/839127.pdf
    Data publikacji:
    1999
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    toxic property
    trichinellosis
    nitric oxide
    morphological transformation
    parasitology
    muscle cell
    signalling property
    infectious disease
    physiological function
    synthase activity
    histochemistry
    Opis:
    Nitric oxide plays a critical role in a variety of biological acfivities. It has been nicknamed a ,,killer'' and ,,mediator" due to its toxic and signalling properties. Apart from its regular physiological function, nitric oxide indirectly participates in infectious diseases. Our report seems to be first presentation of the nitric oxide synthase participation in the host biochemical defence mechanisms and in morphological transformation of muscle cells in trichinellosis.
    Źródło:
    Annals of Parasitology; 1999, 45, 1
    0043-5163
    Pojawia się w:
    Annals of Parasitology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-13 z 13

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