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Wyszukujesz frazę "isoenzyme marker" wg kryterium: Temat


Wyświetlanie 1-8 z 8
Tytuł:
Isozyme polymorphism and seed and cone variability of Scots pine (Pinus sylvestris L.) in relation to local environments in Poland
Autorzy:
Przybylski, Paweł
Masternak, Katarzyna
Jastrzębowski, Szymon
Powiązania:
https://bibliotekanauki.pl/articles/2041636.pdf
Data publikacji:
2020
Wydawca:
Instytut Badawczy Leśnictwa
Tematy:
adaptive capacity
isoenzyme marker
Scots pine
seeds
Opis:
Evolutionary processes lead to the survival of individuals best adapted to local environment. This gives rise to allele polymorphism and genetic diversity of populations. Isoenzyme proteins, which are the product of gene expression, are an effective tool for tracking these changes. On the other hand, the reproductive potential of a given population can be assessed based on its ability to produce viable and efficiently germinating seeds. The present results combine molecular analyses of isoenzyme proteins with anatomical and morphological studies of Scots pine seeds (Pinus sylvestris L.). The study was conducted in 6 populations that are characteristic of this species occurrence range in the country. The results confirm the correlation between seed weight and embryo size. They also show a population from northeastern Poland had a higher effective number of alleles and seed with lower germinative energy and capacity. There was genetic homogeneity in all except for the population from Woziwoda, which was significantly different based on the Fst test. The genetic characteristics of Scots pine from Woziwoda may be associated with the lower levels of rainfall that occur there during the growing season. The results improve our knowledge of Scots pine variability and contribute to the discussion of the impact of local environment on genetic variability.
Źródło:
Folia Forestalia Polonica. Series A . Forestry; 2020, 62, 2; 88-99
0071-6677
Pojawia się w:
Folia Forestalia Polonica. Series A . Forestry
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Identyfing alien genotypes and their consequences for genetic variationin clonal seed orchards of Pinus sylvestris L.
Autorzy:
Przybylski, P.
Kowalczyk, J.
Odrzykoski, I.
Matras, J.
Powiązania:
https://bibliotekanauki.pl/articles/2077755.pdf
Data publikacji:
2019
Wydawca:
Polska Akademia Nauk. Instytut Dendrologii PAN
Tematy:
genetic analysis
Pinus sylvestris
tree breeding
isoenzyme marker
grafting error
Opis:
This study investigates the rates of grafting and planting errors that occur in seed orchards, and evaluates their effects on the genetic diversity and relatedness of genotypes. The study used three clonal Scots pine seed orchards of differing ages and clonal composition located in the forest districts of Susz, Pniewy and Zdrojowa Góra, Poland. Maximum breeding ability within a seed orchard requires isolated from external sources of pollen, and have no alien genotypes inside the orchard. We used 13 isoenzyme markers to determine the genotypic identity of ramets and compared the number of genotypes of the actual current ramet population (W1) with genotypes comprising the originally intended plus trees (designated as W0) to estimate the genotypic assignment error rate per orchard. For both W0 and W1, we calculated the effective number of clones and the relative effective number of clones. Ramet assignment errors were detected in all three seed orchards. Gnotypic errors ranged from 5.8% to 37.7% across orchards. A total of 46 alleles were found, with the mean number of alleles per locus ranging from 2.77 to 3.23. At individual loci, the level of observed heterozygosity was variable. Alien genotypes had negligible effects on seed orchard heterozygosity. The Fst values between seed orchards amounted to 0.6% between Susz and Pniewy and 1.1% between Susz and Zdrojowa Góra. The effect on genetic variation of ramet assignment errors was small and influenced genetic diversity only in the case of the Susz seed orchard. However, our results suggest that negative effects of alien genotypes can occur on breeding value of seeds from analysed seeds orchards.
Źródło:
Dendrobiology; 2019, 81; 40-46
1641-1307
Pojawia się w:
Dendrobiology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Isoenzyme markers of two hepatic species: Barbilophozia lycopodioides [Wallr.] Loeske, and B. hatcheri [A. Evans] Loeske
Autorzy:
Baczkiewicz, A
Buczkowska, K.
Lembicz, M.
Powiązania:
https://bibliotekanauki.pl/articles/57952.pdf
Data publikacji:
2003
Wydawca:
Polskie Towarzystwo Botaniczne
Tematy:
Tatras Mountains
Polska
Barbilophozia hatcheri
plant community
Barbilophozia lycopodioides
isoenzyme marker
population
hepatic species
Opis:
Two closely related species of the genus Barbilophozia: B. lycopodioides and B. hatcheri were studied in populations from the Tatra Range (S Poland), where they are frequent and widely distributed. Both species play an important role in plant communities and grow here very often side by side. Typically developed plants are quite easy to distinguish (even in the field), however morphologically intermediate forms, difficult to recognize by using of classical taxonomic methods, sometimes are found. We found enzymatic markers, that allow to recognize the critical forms. Both studied species are different in enzymatic patterns of glutamate oxaloacetate transaminase (GOT) and peroxidases (PX). In GOT four different phenotypes were detected. The first two (GOT 1 and GOT 2) were characteristic for B. hatcheri and next two (GOT 3 and GOT 4) for B. lycopodioides. Peroxidase patterns, that were monomorphic and specific for each species, exhibit different mobility in anodal and cathodal parts of gel. Results of the studies allowed us to draw the conclusion, that PX and GOT are good isoenzymatic markers and they can have practical application for identification of Barbilophozia species.
Źródło:
Acta Societatis Botanicorum Poloniae; 2003, 72, 2
0001-6977
2083-9480
Pojawia się w:
Acta Societatis Botanicorum Poloniae
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Genetic structure of Picea abies populations growing on extreme sites as revealed by isoenzyme markers: a case study from Slovenia and Bosnia and Herzegovina
Autorzy:
Ballian, D
Bogunic, F.
Bozic, G.
Powiązania:
https://bibliotekanauki.pl/articles/41007.pdf
Data publikacji:
2009
Wydawca:
Polska Akademia Nauk. Instytut Dendrologii PAN
Tematy:
international conference
Europe
forest ecosystem
plant breeding
tree
Norway spruce
Picea abies
plant population
genetic structure
extreme site
isoenzyme marker
genetic variation
gene polymorphism
forest tree
Slovenia
Bosnia and Herzegovina
forest community
Sphagno-Piceetum community
Opis:
Three populations of Norway spruce from ecologically extreme environments in Slovenia and Bosnia and Herzegovina were examined for genetic polymorphism. The spruces there grow in specific forest communities (Sphagno-Piceetum) which represent the remnants of the post-glacial vegetation. The aim of the study was to search for similarities in the genetic variation among populations adapted to such conditions. In total, 10 isoenzyme systems involving 16 gene loci were analysed. The results showed differences in genetic differentiation at loci Got-B, Skdh-A and 6-Pgdh-C between the two Slovenian populations and the Bosnian population, but also indicated an interestingly close relationship between the Slovenian population Pohorje and the Bosnian population Nišići.
Źródło:
Dendrobiology; 2009, 61 Supplement
1641-1307
Pojawia się w:
Dendrobiology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Linkage of two mutant allozymes for Amp2 and Aat2 with marker loci on barley [Hordeum vulgare L.] chromosomes 1 and 6
Autorzy:
Kucharska, M
Kaczorowska, K.
Ali, E.S.
Powiązania:
https://bibliotekanauki.pl/articles/2048291.pdf
Data publikacji:
1998
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
chromosome
starch
isoenzyme
barley
linkage
gel electrophoresis
mutant
allozyme
genetic analysis
Hordeum vulgare
genetic marker
Opis:
To saturate barley (Hordeum vulgare L.) genetic maps the linkage relationships of two isoenzyme loci Amp2 (aminopeptydase) and Aat2 (aspartate aminotransferase) with known genetic markers were investigated. Results of the genetic analysis support previous information on the localization of these loci on chromosome 1 and 6, respectively. The following recombination values were estimated: between locus Amp2 and T1-3b translocation break point 13.8 ± 2.1%, between locus Aat2 and translocation T6-7i, 17 ± 3.0% and between locus Aat2 and marker о 24.1 ± 3.0%.
Źródło:
Journal of Applied Genetics; 1998, 39, 2; 147-150
1234-1983
Pojawia się w:
Journal of Applied Genetics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
How do eyespot resistance genes transferred into winter wheat breeding lines affect their yield?
Autorzy:
Kwiatek, M.
Wisniewska, H.
Korbas, M.
Gawlowska, M.
Belter, J.
Majka, M.
Danielewicz, J.
Powiązania:
https://bibliotekanauki.pl/articles/66314.pdf
Data publikacji:
2016
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
eyespot
resistance gene
wheat
winter wheat
breeding line
yield
inoculation
isoenzyme
molecular marker
plant resistance
Triticum aestivum
Opis:
Eyespot can reduce yields, even up to 50%. There are four genetically characterized resistances in wheat varieties, controlled by: (1) the Pch1 gene, transferred from Aegilops ventricosa; (2) the Pch2 gene, originating from wheat variety Capelle Desprez; (3) the Pch3 gene, originating from Dasypyrum villosum; and (4) the Q.Pch.jic-5A gene, a quantitative trait locus (QTL) located on chromosome 5A of Capelle Desprez. However, those loci have drawbacks, such as linkage of Pch1 with deleterious traits and limited effectiveness of Pch2 against the disease. Here we present an initial study which aims to characterize wheat pre-registration breeding lines carrying 12 eyespot resistance genes, consider their resistance expression in inoculation tests and the influence of resistance genotypes on the yield. We selected four groups of breeding lines, carrying: (1) the Pch1 gene alone: one line; (2) the Pch2 gene alone: four lines; (3) the Q.Pch.jic-5A gene alone: one line; and (4) Pch1 + Q.Pch.jic-5A: three lines. For the first time, the effect of the combination of Pch1 and Q.Pch.jic-5A genes was compared with resistance conferred by Pch1 or Q.Pch.jic-5A alone. We found significant differences between infection scores evaluated in resistant lines carrying Pch1 and Q.Pch.jic-5A alone, while no differences in terms of the level of resistance expression were detected between Pch1 alone and Pch1 + Q.Pch.jic-5A, and between wheat lines carrying Pch1 and Pch2 alone. Moreover, we demonstrated that the Pch1 gene, together with an Ae. ventricosa segment, caused statistically significant yield losses, both as a single eyespot resistance source or in a combination with Q.Pch.jic-5A. Yield scores showed that wheat lines with Q.Pch.jic-5A had the highest yields, similar to the yielding potential of Pch2-bearing lines and control varieties.
Źródło:
Journal of Plant Protection Research; 2016, 56, 4
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Genetic variation of Picea abies in southern Germany as determined using isozyme and STS markers
Autorzy:
Konnert, M
Powiązania:
https://bibliotekanauki.pl/articles/40969.pdf
Data publikacji:
2009
Wydawca:
Polska Akademia Nauk. Instytut Dendrologii PAN
Tematy:
international conference
Europe
forest ecosystem
plant breeding
tree
Norway spruce
Picea abies
genetic variation
Germany
isoenzyme
Bavaria
DNA marker
plant genetics
provenance
Opis:
Over 50 populations of Norway spruce from Bavaria were analysed at 23 isozyme gene loci. The mean genetic distances between these populations were quite small. A geographical grouping could not be observed, and discrimination between provenances from high and low altitudes was not identifiable using this marker type, either. The only difference between spruce populations from South Bavaria and those from Northeast Bavaria is in the presence of some distinct rare alleles. The highest values for the genetic diversity were detected for spruce stands in Northeast Bavaria (Frankonian Forest). Using STS markers, further genes of the nuclear genome of Picea abies can be dealt with. The genetic differences found on the basis of ten STS markers between different Picea abies seed lots and/or seedling populations are generally 2-3 times greater than those found by means of isozyme gene markers. DNA markers turned out to be an appropriate and substantial addition or even more a suitable alternative to isozyme markers for analysing genetic variation and testing provenance identity. Their advantages consist in a markedly wider variation as well as in the enlarged genome segments investigated.
Źródło:
Dendrobiology; 2009, 61 Supplement
1641-1307
Pojawia się w:
Dendrobiology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Isozyme and RAPD markers for the identification of pea, field bean and lupin cultivars
Autorzy:
Wolko, B
Swiecicki, W.K.
Kruszka, K.
Irzykowska, L.
Powiązania:
https://bibliotekanauki.pl/articles/2043430.pdf
Data publikacji:
2000
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Lupinus angustifolius
legume crop
isoenzyme
morphological marker
electrophoresis
breeding selection
allozyme
Lupinus albus
seed
lupin cultivar
pea cultivar
field bean
polymorphism
Lupinus luteus
germ plasm
Vicia faba var.minor
enzyme system
cultivar identification
DNA
Pisum sativum
Źródło:
Journal of Applied Genetics; 2000, 41, 3; 151-165
1234-1983
Pojawia się w:
Journal of Applied Genetics
Dostawca treści:
Biblioteka Nauki
Artykuł
    Wyświetlanie 1-8 z 8

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