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    Wyświetlanie 1-12 z 12
    Tytuł:
    Pigmented Maize (Zea mays L.) Contains Anthocyanins with Potential Therapeutic Action Against Oxidative Stress - A Review
    Autorzy:
    Magaña Cerino, Jesús M.
    Peniche Pavía, Héctor A.
    Tiessen, Axel
    Gurrola Díaz, Carmen M.
    Powiązania:
    https://bibliotekanauki.pl/articles/1369589.pdf
    Data publikacji:
    2020
    Wydawca:
    Instytut Rozrodu Zwierząt i Badań Żywności Polskiej Akademii Nauk w Olsztynie
    Tematy:
    Zea mays L
    anthocyanins
    Oxidative stress
    Antioxidant
    in vivo and in vitro effect
    Opis:
    Different maize (Zea mays L.) varieties have been used for thousands of years as a healthy food source in Mesoamerica including pigmented maize. Maize ingestion could contribute to the reduction in the rate of non-communicable diseases and, in turn, to its function as an adjuvant in their management. These diseases are mainly associated with oxidative stress, which is characterized by a redox cell imbalance produced due to pro-oxidant molecules accumulation, inducing irreversible damages. Although the endogenous antioxidant defense system is efficient, exogenous antioxidants are necessary to help to prevent this damage. Bioactive compounds, like anthocyanins, contained in dietary plants exert a major activity against oxidative stress. Could the maize anthocyanins play a curative, preventive or complementary role in the treatment of chronic diseases? Here, we describe the occurrence of anthocyanins from pigmented maize and their chemical structures. Furthermore, the biosynthesis, bioavailability, and stability are also summarized. Finally, many in vitro and in vivo studies of maize anthocyanins are discussed that demonstrated their nutraceutical potential, antioxidant capacity, and other biological effects. Given the importance of the biological properties of maize anthocyanins, it is necessary to understand the current knowledge and propose further research or clinical studies which allows us to better elucidate the biological mechanism of maize anthocyanins derivatives of several varieties and processes of cooking and combination with other ingredients to enhance their nutritional and health benefits.
    Źródło:
    Polish Journal of Food and Nutrition Sciences; 2020, 70, 2; 85-99
    1230-0322
    2083-6007
    Pojawia się w:
    Polish Journal of Food and Nutrition Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    In vitro effect of antiparasitic drugs on the expression of the genes code receptors: AchR and GABA in Anisakis simplex L3 larvae
    Autorzy:
    Lopienska-Biernat, E.
    Polak, I.
    Stryiński, R.
    Powiązania:
    https://bibliotekanauki.pl/articles/6514.pdf
    Data publikacji:
    2016
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    in vitro effect
    antiparasitic drug
    gene expression
    receptor
    AchR zob.acetylcholine receptor
    acetylcholine receptor
    gamma-aminobutyric acid
    Anisakis simplex
    larva
    Źródło:
    Annals of Parasitology; 2016, 62, Suppl.
    0043-5163
    Pojawia się w:
    Annals of Parasitology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The in vitro effect of bovine lactoferrin on the activity of organ leukocytes in rainbow trout [Oncorhynchus mykiss], European eel [Anguilla anguilla] and wels catfish [Silurus glanis]
    Autorzy:
    Malaczewska, J
    Wojcik, M.
    Wojcik, R.
    Siwicki, A.K.
    Powiązania:
    https://bibliotekanauki.pl/articles/30969.pdf
    Data publikacji:
    2010
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    fish
    rainbow trout
    Oncorhynchus mykiss
    European eel
    Anguilla anguilla
    catfish
    Silurus glanis
    in vitro effect
    bovine lactoferrin
    lactoferrin
    leucocyte
    freshwater fish
    Opis:
    Lactoferrin (LF) is a glycoprotein found in milk, neutrophil granules, secretions and selected organs of mammals. Lactoferrin exhibits antibacterial, antiviral, fungicidal, immunoregulatory and other functions. Although fish are devoid of this protein and its cell receptors, LF effect on the immune mechanisms of fish has been demonstrated. The objective of this study was to investigate the effect of bovine lactoferrin, applied in vitro, on the activity of head kidney and spleen leukocytes in three freshwater fish species: rainbow trout (Oncorhynchus mykiss), European eel (Anguilla anguilla) and weis catfish (Silurus glanis). The obtained results validate LF beneficial effect on the respiratory burst of phagocytes in rainbow trout and weis catfish despite the fact that the potential killing activity against Aeromonas hydrophila was not stimulated in any of the studied species. Bovine lactoferrin enhanced the proliferation of T-lymphocytes in rainbow trout and European eel, as well as of B-lymphocytes in rainbow trout.
    Źródło:
    Polish Journal of Veterinary Sciences; 2010, 13, 1; 83-88
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Micropropagation and in vitro rooting of Robinia pseudoacacia L. recalcitrant genotypes
    Autorzy:
    Szyp-Borowska, Iwona
    Ukalska, Joanna
    Wojda, Tomasz
    Sułkowska, Małgorzata
    Klisz, Marcin
    Powiązania:
    https://bibliotekanauki.pl/articles/2041685.pdf
    Data publikacji:
    2020
    Wydawca:
    Instytut Badawczy Leśnictwa
    Tematy:
    black locust
    in vitro cultures
    medium effect
    Opis:
    In forest production, there is an emerging tendency towards the planting of fast-growing trees as attractive, renewable energy sources. Hence, efforts were made to develop a method of micropropagation by organogenesis of seven clones of black locust (Robinia pseudoacacia L.) that are resistant to propagation by traditional vegetative methods, as well as one plus tree (no. 9755) at the age of 60, to see if the age of the mother plant is a limitation in the micropropagation of black locust trees. Overall results suggest that Murashige and Skoog medium supplemented with 30 g l−1 sucrose, 0.6 mg l−1 6-benzylaminopurine (BAP) and 0.1 mg l−1 naphthalene acetic acid (NAA) is better for the propagation of each genotype of R. pseudoacacia than Woody Plant Medium with the same growth regulators, and the age of the donor plant does not affect the organogenic potential. Recalcitrance to adventitious rooting from adventitious shoot formation is a major limitation for the clonal micropropagation of forest trees. Our results showed that although the roots were also formed spontaneously in the growth medium without growth hormones for the tested black locust clones, the application of auxin increased the total root length compared to that in the medium with active carbon and control. A significant effect of the additives of hormone and sucrose on the total root length was found. Increasing the sucrose concentration stimulated the induction of roots in each of the tested concentrations (5, 10, 15 or 20 g l−1). Additionally, the change in sugar dose in the rooting medium caused significant differences in total root length.
    Źródło:
    Folia Forestalia Polonica. Series A . Forestry; 2020, 62, 1; 13-21
    0071-6677
    Pojawia się w:
    Folia Forestalia Polonica. Series A . Forestry
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Side effects of fungicides and insecticides on entomopathogenic fungi in vitro
    Autorzy:
    Fiedler, Z.
    Sosnowska, D.
    Powiązania:
    https://bibliotekanauki.pl/articles/65735.pdf
    Data publikacji:
    2017
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    entomopathogenic fungi
    colony growth
    side effect
    fungicide
    insecticide
    in vitro
    sporulation
    Opis:
    Products based on different strains of entomopathogenic fungi are now being used in Integrated Pest Management (IPM) programs. Compatibility studies of chemical and biological control agents are necessary to be able to give proper recommendations for their integrated use. The effect of three insecticides based on imidacloprid, spinosad and abamectin, and three fungicides based on chlorothalonil, azoxystrobin and thiophanate-methyl on the activity of the following entomopathogenic fungi: Metarhizium anisopliae (Metschn.) sensu lato, Beauveria bassiana (Bals.-Criv.) Vuill., Acremonium sp. was tested under laboratory conditions. Tests of the influence of the pesticides on growth and production of conidia were performed. From this study, we concluded that all tested insecticides can be applied together with fungus B. bassiana products in IPM programs. They even stimulate sporulation of this fungus at the recommended dose, 0.5 of the recommended dose and 1.5 times the recommended dose. In the case of fungicides we observed inhibition of growth and sporulation of B. bassiana and reduction of growth and sporulation of other species of fungi.
    Źródło:
    Journal of Plant Protection Research; 2017, 57, 4
    1427-4345
    Pojawia się w:
    Journal of Plant Protection Research
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The inhibitory effect of tropolone and hinokitiol on the mycelium growth of Phoma narcissi in vitro
    Hamujace dzialanie tropolone i hinokitiolu na wzrost grzybni Phoma narcissi in vitro
    Autorzy:
    Saniewska, A
    Saniewski, M
    Powiązania:
    https://bibliotekanauki.pl/articles/28161.pdf
    Data publikacji:
    2007
    Wydawca:
    Polskie Towarzystwo Botaniczne
    Tematy:
    pathogen
    in vitro
    inhibitory effect
    tropolone
    Phoma narcissi
    Hippeastrum
    hinokitiol
    Amaryllidaceae
    mycelium growth
    Opis:
    Tropolone and hinokitiol (β-thujaplicin) that are present in heartwood of several Cupressaceae trees are known for their antibacterial, antifungal and insecticidal properties. In the present studies it was showed that tropolone and hinokitiol greatly inhibited in vitro, on PDA medium, the mycelium growth of Phoma narcissi, a pathogen of Hippeastrum and other species of family Amaryllidaceae. Total inhibition of the mycelium growth of Phoma narcissi took place at a tropolone concentration of 6.0 µg·cm⁻³ and at a hinokitiol concentration of 50.0 µg·cm⁻³. Fungicidal doses of tropolone and hinokitiol for the mycelium growth of Phoma narcissi were also documented. The results presented in this paper are discussed with data available in literature on the antifungal action of tropolone and hinokitiol on other species of pathogenic fungi.
    Tropolone i hinokitiol (β-tujaplicyna), występujące w wielu gatunkach drzew należących do rodziny Cupressaceae, mają właściwości antybakteryjne, antygrzybowe i insektycidalne. W obecnych badaniach wykazano, że tropolone i hinokitiol silnie hamowały in vitro na pożywce PDA wzrost grzybni Phoma narcissi, patogena Hippeastrum i innych gatunków z rodziny Amaryllidaceae. Całkowite zahamowanie wzrostu grzybni Phoma narcissi następowało przy stężeniu tropolone 6,0 µg·cm⁻³ i przy stężeniu hinokitiolu 50,0 µg·cm⁻³. Fungicidalne stężenia tropolone i hinokitiolu zostały również określone. Wyniki badań własnych zostały przedyskutowane z dostępnymi danymi w literaturze o antygrzybowym działaniu tropolone i hinokitiolu na inne gatunki grzybów patogenicznych.
    Źródło:
    Acta Agrobotanica; 2007, 60, 1
    0065-0951
    2300-357X
    Pojawia się w:
    Acta Agrobotanica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Optimizing sterilization conditions and growth regulator effects on in vitro shoot regeneration through direct organogenesis in Chenopodium quinoa
    Autorzy:
    Hesami, M.
    Naderi, R.
    Yoosefzadeh-Najafabadi, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/80105.pdf
    Data publikacji:
    2018
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    Chenopodium quinoa
    organogenesis
    cotyledon
    shoot regeneration
    in vitro propagation
    growth regulator effect
    sterilization
    Źródło:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2018, 99, 1
    0860-7796
    Pojawia się w:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Evaluation of in vitro effect of selected contact lens solutions conjugated with nanoparticles in terms of preventive approach to public health risk generated by Acanthamoeba strains
    Autorzy:
    Padzik, M.
    Hendiger, E.B.
    Żochowska, A.
    Szczepaniak, J.
    Baltaza, W.
    Pietruczuk-Padzik, A.
    Olędzka, G.
    Chomicz, L.
    Powiązania:
    https://bibliotekanauki.pl/articles/2082951.pdf
    Data publikacji:
    2019
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    Acanthamoeba keratitis
    contact lens solutions
    silver/gold nanoparticles
    Acanthamoeba trophozoites
    anti-amoebic in
    vitro effect
    Opis:
    Introduction. Various Acanthamoeba species are free-living organisms widely distributed in the human environment. Amphizoic amoebae as facultative parasites may cause vision-threatening eye disease – Acanthamoeba keratitis, mostly among contact lens wearers. As the number of cases is increasing, and applied therapy often unsuccessful, proper hygienic measures and effective contact lenses disinfection are crucial for the prevention of this disease. Available contact lens solutions are not fully effective against amphizoic amoebae; there is a need to enhance their disinfecting activity to prevent amoebic infections. The use of developing nanotechnology methods already applied with success in the prevention, diagnostic and therapy of other infectious diseases might be helpful regarding amoebic keratitis. This study assesses the in vitro effect of selected contact lens solutions conjugated with nanoparticles against Acanthamoeba trophozoites. Materials and method. Three selected contact lens solutions conjugated with silver and gold nanoparticles in concentration of 0.25–2.5 ppm were used in vitro against the axenically cultured ATCC 30010 type Acanthamoeba castellanii strain. The anti-amoebic efficacy was examined based on the oxido-reduction of AlamarBlue. The cytotoxicity tests based on the measurement of lactate dehydrogenase (LDH) activity were performed using a fibroblast HS-5 cell line. Results. Enhancement of the anti-amoebic activity of contact lens solutions conjugated with selected nanoparticles expressed in the dose dependent amoebic growth inhibition with a low cytotoxicity profile was observed. Conclusions. Results of the study showed that conjugation of selected contact lens solutions with silver nanoparticles might be a promising approach to prevent Acanthamoeba keratitis among contact lens users.
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2019, 26, 1; 198-202
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    In vitro and in vivo activities of flavonoids - apigenin, baicalin, chrysin, scutellarin - in regulation of hypertension - a review for their possible effects in pregnancy-induced hypertension
    Autorzy:
    Ozarowski, M.
    Kujawski, R.
    Mikolajczak, P.L.
    Wielgus, K.
    Klejewski, A.
    Wolski, H.
    Seremak-Mrozikiewicz, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/72144.pdf
    Data publikacji:
    2019
    Wydawca:
    Instytut Włókien Naturalnych i Roślin Zielarskich
    Tematy:
    in vitro model
    in vivo model
    flavonoids
    pharmacological activity
    apigenin
    baicalin
    chrysin
    scutellarin
    regulation
    hypertension
    possible effect
    pregnancy induced hypertension
    animal model
    Opis:
    Flavonoids and their conjugates are the most important group of natural chemical compounds in drug discovery and development. The search for pharmacological activity and new mechanisms of activity of these chemical compounds, which may inhibit mediators of inflammation and influence the structure and function of endothelial cells, can be an interesting pharmacological strategy for the prevention and adjunctive treatments of hypertension, especially induced by pregnancy. Because cardiovascular diseases have multifactorial pathogenesis these natural chemical compounds with wide spectrum of biological activities are the most interesting source of new drugs. Extracts from one of the most popular plant used in Traditional Chinese Medicine, Scutellaria baicalensis Georgi could be a very interesting source of flavonoids because of its exact content in quercetin, apigenin, chrysin and scutellarin as well as in baicalin. These flavonoids exert vasoprotective properties and many activities such as: anti-oxidative via several pathways, anti-inflammatory, anti-ischaemic, cardioprotective and anti-hypertensive. However, there is lack of summaries of results of studies in context of potential and future application of flavonoids with determined composition and activity. Our review aims to provide a literature survey of in vitro, in vivo and ex vivo pharmacological studies of selected flavonoids (apigenin, chrysin and scutellarin, baicalin) in various models of hypertension carried out in 2008–2018.
    Źródło:
    Herba Polonica; 2019, 65, 1
    0018-0599
    Pojawia się w:
    Herba Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Antimicrobial effects of Citrus sinensis peel extracts against periodontopathic bacteria: an in vitro study
    Autorzy:
    Hussain, K.A.
    Tarakji, B.
    Kandy, B.P.P.
    John, J.
    Mathews, J.
    Ramphul, V.
    Divakar, D.D.
    Powiązania:
    https://bibliotekanauki.pl/articles/872640.pdf
    Data publikacji:
    2015
    Wydawca:
    Narodowy Instytut Zdrowia Publicznego. Państwowy Zakład Higieny
    Tematy:
    antimicrobial effect
    Citrus sinensis
    peel extract
    periodontopathic bacteria
    periodontal disease
    Aggregatibacter actinomycetemcomitans
    Prevotella intermedia
    Porphyromonas gingivalis
    in vitro study
    Opis:
    Background. Use of plant extracts and phytochemicals with known antimicrobial properties may have great significance in therapeutic treatments. Objective. To assess the in vitro antimicrobial potential and also determine the minimum inhibitory concentration (MIC) of Citrus sinensis peel extracts with a view of searching a novel extract as a remedy for periodontal pathogens. Materials and Methods. Aqueous and ethanol (cold and hot) extracts prepared from peel of Citrus sinensis were screened for in vitro antimicrobial activity against Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis and Prevotella intermedia, using agar well diffusion method. The lowest concentration of every extract considered as the minimal inhibitory concentration (MIC) values were determined for both test organisms. Confidence level and level of significance were set at 95% and 5% respectively. Results. Prevotella intermedia and Porphyromonas gingivalis were resistant to aqueous extracts while Aggregatibacter actinomycetemcomitans was inhibited at very high cncentrations. Hot ethanolic extracts showed significantly higher zone of inhibition than cold ethanolic extract. Minimum inhibitory concentration of hot and cold ethanolic extracts of Citrus sinensis peel ranged between 12-15 mg/ml against all three periodontal pathogens. Conclusions. Both extracts were found sensitive and contain compounds with therapeutic potential. Nevertheless, clinical trials on the effect of these plants are essential before advocating large-scale therapy.
    Źródło:
    Roczniki Państwowego Zakładu Higieny; 2015, 66, 2
    0035-7715
    Pojawia się w:
    Roczniki Państwowego Zakładu Higieny
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    In vitro effects of fipronil on neuronal excitability in mammalian and molluscan nervous systems
    Autorzy:
    Varro, P
    Gyori, J.
    Vilagi, I.
    Powiązania:
    https://bibliotekanauki.pl/articles/49291.pdf
    Data publikacji:
    2009
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    nervous system
    mammal
    mollusc
    fipronil effect
    in vitro
    neuronal excitability
    rat
    Lymnaea stagnalis
    electrophysiology
    gamma-aminobutyric acid receptor
    insecticide
    veterinary medicine
    dog
    cat
    flea
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2009, 16, 1; 71-77
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    A new and fast method to obtain in vitro cultures of Huperzia selago (Huperziaceae) sporophytes, a club moss which is a source of huperzine A
    Autorzy:
    Szypula, W.J.
    Mistrzak, P.
    Olszowska, O.
    Powiązania:
    https://bibliotekanauki.pl/articles/58228.pdf
    Data publikacji:
    2013
    Wydawca:
    Polskie Towarzystwo Botaniczne
    Tematy:
    clubmoss
    ground pine zob.clubmoss
    club moss zob.clubmoss
    in vitro culture
    Huperzia selago
    Lycopodium selago
    Huperziaceae
    sporophyte
    huperzine A
    sesquiterpene alkaloid
    pharmaceutical effect
    treatment
    Opis:
    This study presents a protocol for a fast and effective in vitro axenic culture of Huperzia selago (Huperziaceae Rothm.) sporophytes, a club moss which is a source of huperzine A, an alkaloid of a considerable therapeutic potential extensively investigated for its uses as treatment for some neurodegenerative diseases. The proposed procedure allowed approximately tenfold shortening of the species developmental stages with the omission of the gametophyte stage while the sporophyte mass could be increased tenfold within a 6-month period. The cultures were established using vegetative propagules (bulbils) procured from sporophytes growing in the wild without degrading the habitats of this endangered plant species. Explants underwent surface and internal disinfection to eliminate the epiphytic and endophytic bacteria and fungi. In in vitro cultures, the optimum results were achieved using Moore (Mr) medium without growth regulators or supplemented with 0.015 mg/l IBA and 0.3 mg/l kinetin. These media ensured both viability of the propagules and their further development. The biomass growth index for H. selago sporophytes grown from propagules, determined at 3 months of culture (1 passage) on Mr medium with IBA and kinetin was 650%. At 6 months, the biomass growth index increased to 1114%. Vigorous growth of adventitious roots, especially on Mr medium with the addition of 0.25 mg/l NAA, and callus formation on shoot apices were observed. At 6 months of culture, some sporophytes obtained from the bulbils were used as the initiating material for shoot subcultures, which developed best on Mr medium with IBA and kinetin.
    Źródło:
    Acta Societatis Botanicorum Poloniae; 2013, 82, 4
    0001-6977
    2083-9480
    Pojawia się w:
    Acta Societatis Botanicorum Poloniae
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-12 z 12

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