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Tytuł:
Helicobacter pylori infection in North-East Poland between 1996 and 2011 on the base of histological examination
Autorzy:
Namiot, A.
Chilewicz, M.
Kurylonek, A. J.
Skwarski, L.
Romatowski, J
Killar, G.
Jaroszewicz, W.
Kralisz, M.
Chętnik, A.
Bucki, R.
Kemona, A.
Namiot, Z.
Powiązania:
https://bibliotekanauki.pl/articles/1918587.pdf
Data publikacji:
2018-06-18
Wydawca:
Uniwersytet Medyczny w Białymstoku
Tematy:
Helicobacter pylori
histology
gastric mucosa
Opis:
Purpose: The aim of the study was to conduct a 15- year (1996-2011) observations on the frequency of Helicobacter pylori stomach infection in patients having performed gastroscopy in the Endoscopy Unit District Hospital of Białystok, Poland Materials and methods: Out of the 27421 patients who underwent a gastroscopy in 1996-1997, 2000- 2001, 2005-2006, and 2010-2011 years were selected 4216 subjects who had performed histological examination of gastric mucosal specimens for H. pylori infection. The mucosal specimens after placed in buffered formalin, were subjected to standard histological procedure, and stained with hematoxylineosin and Giemsa. Results: Stomach infection with H. pylori was 73.36% (1996-1997), 48.60% (2000-2001), 33.61% (2005-2006) and 32.30% (2010-2011); only between the last two 2-year intervals the difference was not significant. Conclusions: The results of current study indicate on steadily declining stomach infection with H. pylori. Keywords: Helicobacter pylori, histology, gastric mucosa
Źródło:
Progress in Health Sciences; 2018, 8(1); 22-26
2083-1617
Pojawia się w:
Progress in Health Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Vitamin C does not improve the efficacy of Helicobacter pylori eradication in smokers
Autorzy:
Namiot, A.
Namiot, D.B.
Bucki, R.
Kemona, A.
Kurylonek, A.J.
Namiot, Z.
Powiązania:
https://bibliotekanauki.pl/articles/1918926.pdf
Data publikacji:
2020-06-10
Wydawca:
Uniwersytet Medyczny w Białymstoku
Tematy:
Helicobacter pylori
treatment
vitamin C
Opis:
Purpose: To evaluate the hypothesis that vitamin C improves the efficacy of Helicobacter pylori eradication in smokers when combined with pantoprazole, amoxicillin, and clarithromycin. Materials and methods: The study completed 90 subjects of 98 enrolled, 58 smokers 32 nonsmokers. Helicobacter pylori status was determined by two methods, CLO test, and histology. Vitamin C (500 mg) was administered three times daily. The patients were considered as cured of H. pylori if the CLO test result and histology were negative 4 weeks after completion of eradication therapy. Results: Smokers had lower effectiveness of eradication therapy than non-smokers and the administration of vitamin C had not affected the outcome of eradication therapy. Conclusions: Vitamin C (500 mg tid) does not improve the eradication therapy when pantoprazole, amoxicillin, and clarithromycin were used.
Źródło:
Progress in Health Sciences; 2020, 10(1); 77-81
2083-1617
Pojawia się w:
Progress in Health Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The microbiological, histological, immunological and molecular determinants of Helicobacter pylori infection in guinea pigs as a convenient animal model to study pathogenicity of these bacteria and the infection dependent immune response of the host
Autorzy:
Walencka, Maria
Gonciarz, Weronika
Mnich, Eliza
Gajewski, Adrian
Stawerski, Pawel
Knapik-Dabrowicz, Alina
Chmiela, Magdalena
Powiązania:
https://bibliotekanauki.pl/articles/1038889.pdf
Data publikacji:
2015
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
Helicobacter pylori
guinea pigs
diagnostic procedures
Opis:
Helicobacter pylori is an etiological agent of chronic gastritis, gastric and duodenal ulcers and gastric cancers. The use of an appropriate animal model for experimental studies on the pathogenesis of H. pylori infections is necessary due to the chronic character of such infections and difficulties in identifying their early stage in humans. The aim of this study was to develop a guinea pig model of H. pylori infection and identify its microbiological, histological, serological and molecular determinants. Guinea pigs were inoculated per os with H. pylori strains: CCUG 17874 or ATCC 700312, both producing vacuolating cytotoxin A (VacA) and cytotoxin associated gene A (CagA) protein, suspended in Brucella broth with fetal calf serum (FCS) and Skirrow supplement of antibiotics. To determine H. pylori colonization, 7 and 28 days after the challenge, a panel of diagnostic methods was used. It included culturing of microorganisms from the gastric tissue, histopathological analysis of gastric sections, stained by Mayer,s haematoxylin and eosin to assess inflammatory response, by Giemsa as well as Warthin-Starry silver staining to visualise Helicobacter-like organisms (HLO) and with anti-Ki-67 antigen to assess epithelial cell proliferation. H. pylori infection was also confirmed by polymerase chain reactions (PCR) for detection in gastric tissue of ureC and cagA genes and by serological assessment of H. pylori antigens in faeces. This study showed the usefulness of microbiological, histological, immunological and molecular methods for the detection of persistent H. pylori infections in guinea pigs, which could be an appropriate model for studying H. pylori pathogenesis and the related immune response against these microbes.
Źródło:
Acta Biochimica Polonica; 2015, 62, 4; 697-706
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Application of Helicobacter pylori antigen test to evaluate gastric mucosa specimens
Autorzy:
Namiot, A.
Leszczyńska, K.
Namiot, D.B.
Chilewicz, M.
Bucki, R.
Kemona, A
Namiot, Z.
Powiązania:
https://bibliotekanauki.pl/articles/1916576.pdf
Data publikacji:
2014
Wydawca:
Uniwersytet Medyczny w Białymstoku
Tematy:
Helicobacter pylori antigens
immunoassay
gastric mucosa
Opis:
Purpose: To investigate, whether the test documenting the presence of Helicobacter pylori (H. pylori) antigens in the gastric mucosa may be used as diagnostic test. Materials and methods: Mucosal specimens taken from eighty-three patients during gastroscopic examination were subjected to rapid urease test (CLO test), histology, and H. pylori culture. The same biopsy specimens that had been evaluated in the CLO test or collected into the transport medium for bacterial culture were used to detect H. pylori antigens. An amplified immunoassay for the detection of H. pylori antigens in stool was used for gastric mucosa specimens. The sensitivity and specificity of the H. pylori antigen test were evaluated in relation to the results of each verifying test (CLO test, histology, culture) separately and to all 3 tests analysed together. Results: The sensitivity and specificity of the H. pylori antigen test in relation to the CLO test, histological examination, and H. pylori culture were 85.4% and 90.5%, 76.1% and 83.4%, and 90.7% and 90.0% for specimens taken for the CLO test and 90.0% and 82.0%, 78.0% and 81.0%, and 93.0% and 88.0% for specimens taken for bacterial culture, respectively. The sensitivity and specificity of the antigen test in relation to all 3 verifying tests analysed together were 91.3% and 97.3% for specimens taken for the CLO test, and 91.7% and 97.1% for specimens taken for bacterial culture, respectively. Conclusions: H. pylori antigen test in gastric mucosa specimens may be a sufficiently reliable source of information about stomach infection.
Źródło:
Progress in Health Sciences; 2014, 4, 2; 52-57
2083-1617
Pojawia się w:
Progress in Health Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Frequency and immunological consequences of Helicobacter pylori and intestinal parasite co-infections: a brief review
Autorzy:
Krzyżek, Paweł
Gościniak, Grażyna
Powiązania:
https://bibliotekanauki.pl/articles/972171.pdf
Data publikacji:
2017
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
co-infection
helminths
protozoa
Helicobacter pylori
Opis:
Helicobacter pylori is a Gram-negative, spiral bacterium capable of colonizing the gastric mucosa. Infections caused by this microorganism often lead to the development of various gastrointestinal complaints. Simultaneous human colonization by H. pylori and intestinal parasites is a common phenomenon. Moreover, the two groups of pathogens share the similar predisposing factors. The presence of parasites together with H. pylori can significantly influence the modulation of the host immune response. During H. pylori infection, strong polarization of Th1 cells is observed. The presence of protozoa, also contributing to the recruitment of Th1 cells, may well aggravate this response and exacerbate gastric mucosal damage. In contrast, intestinal helminth infection is associated with the polarization of lymphocytes towards Th2; their presence enhances the regenerative processes within the digestive tract and lowers the host overresponse. A literature review suggests that co-infection with intestinal helminths may serve as a buffering mechanism against the effects of H. pylori and/or protozoan infection, alleviating the Th1-dependent response and protecting against inflammations within the gastrointestinal tract.
Źródło:
Annals of Parasitology; 2017, 63, 4; 255-263
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Reflux esophagitis and Helicobacter pylori: Is there an association in children?
Autorzy:
Zagorski, SE.
Nazarenko, ON.
Powiązania:
https://bibliotekanauki.pl/articles/1914060.pdf
Data publikacji:
2014
Wydawca:
Uniwersytet Medyczny w Białymstoku
Tematy:
reflux esophagitis
helicobacter pylori infection
children
Opis:
Purpose: The role of Helicobacter pylori (Hp) in the development of gastroesophageal reflux disease (GERD) remains disputable. This study was planned to determine clinical-epidemic correlations between reflux esophagitis (RE) and Hp in older children. Materials and methods: 308 children with RE and 418 patients with chronic gastritis (CG) without RE between the ages of 12 and 18 were examined. Hp was diagnosed by histological (Giemsa and hematoxylin-eosin stain, with evaluation of dissemination grade) and rapid urease test. Subjective symptoms (heartburn, abdominal pain, other dyspeptic complaints) were analyzed by questionnaire. Results: Hp infection was found in 44.5% of children with RE (no difference in patients without RE), and it does not increase the erosive esophageal defects risk. Clinical symptoms are not connected to this infection in examined patients. The frequency of Hp infection decreases with the disease duration increase. Conclusion: The development of RE is not connected with Hp, but the disease course has certain peculiarities in the conditions of Hp infection.
Źródło:
Progress in Health Sciences; 2014, 4, 1; 41-46
2083-1617
Pojawia się w:
Progress in Health Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Antigen-specific lymphocyte proliferation as a marker of immune response in guinea pigs with sustained Helicobacter pylori infection
Autorzy:
Miszczyk, Eliza
Walencka, Maria
Rudnicka, Karolina
Matusiak, Agnieszka
Rudnicka, Wiesława
Chmiela, Magdalena
Powiązania:
https://bibliotekanauki.pl/articles/1039292.pdf
Data publikacji:
2014
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
Helicobacter pylori
T cells
proliferation
guinea pigs
Opis:
Helicobacter pylori (H. pylori) bacteria are human pathogens causing symptomatic gastritis, peptic ulcer or gastric cancer. Little is known about the kinetics of immune responses in H. pylori infected patients because the initial moment of infection has not been identified. Various animal models are used to investigate the immune processes related to H. pylori infection. In this study we checked whether H. pylori infection in guinea pigs, mimicking natural H. pylori infection in humans, resulted in the development of specific immune responses to H. pylori antigens by measuring the proliferation of lymphocytes localized in mesenteric lymph nodes, spleen and peripheral blood. The maturity of macrophages and cytokines, delivered by monocyte-macrophage lineage or lymphocytes, were considered as mediators, which might influence the lymphocyte blastogenic response. The obtained results showed the activation of T cells localized in mesenteric lymph nodes by H. pylori antigens in H. pylori infected guinea pigs four weeks postinfection. The blastogenic activity of lymphocytes was shaped by their interaction with antigen presenting cells, which were present in the cell cultures during the whole culture period. Moreover, the balance between cytokines derived from adherent leukocytes including interleukin 8 - IL-8 as well as interferon gamma - IFN-γ, and transforming growth factor beta - TGF-β delivered by lymphocytes, was probably important for the successful proliferation of lymphocytes. The H. pylori specific lymphocytes were not propagated in peripheral blood and spleen of H. pylori infected animals. The modulation of immunocompetent cells by H. pylori antigens or their different distribution cannot be excluded.
Źródło:
Acta Biochimica Polonica; 2014, 61, 2; 295-303
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The clinical importance of Helicobacter pylori antigens detected in the dental plaque and feces
Autorzy:
Namiot, A.
Leszczyńska, K.
Namiot, D.
Bucki, R.
Kemona, A.
Chilewicz, M.
Namiot, Z.
Powiązania:
https://bibliotekanauki.pl/articles/1917520.pdf
Data publikacji:
2015
Wydawca:
Uniwersytet Medyczny w Białymstoku
Tematy:
Helicobacter pylori antigens
Immunoassay
Dental Plaque
Feces
Opis:
Purpose:It is expected that H. pylori residing outside the stomach influences the results of the stool test. The aim of the study was to investigate the occurrence of H. pylori antigens in dental plaque and feces of the patients with H. pylori infected and non-infected stomachs.Materials and methods: The study was conducted in 188 dentate patients, 107 with H. pylori infected and 81 non-infected stomachs. Stomach infection with H. pylori was evaluated with CLO test, histology and culture. The stomach was classified as infected if at least two of three tests (CLO test, culture, histology) were positive and as non-infected if all three tests were negative. Dental plaque was taken only from the natural teeth. On the day of the gastroscopic examination or on the next two days a stool sample was collected for H. pylori antigens testing. H. pylori antigens in dental plaque and feces were determined by immunological method.Results:In 60.8% of subjects with an infected stomach, H. pylori antigens were present both in the dental plaque and feces, in 37.4% in feces only, in 0.9% only in the dental plaque, and in 0.9% neither in the dental plaque nor feces. In 46.9% of subjects with a non-infected stomach, H. pylori antigens were found neither in the dental plaque nor feces, in 24.7% both in the dental plaque and feces, in 23.5% only in the dental plaque, and in 4,9% only in feces.Conclusions: There is a weak association between the occurrence of H. pylori antigens in feces and the dental plaque, and also between the occurrence of the antigens in the dental plaque and stomach infection.
Źródło:
Progress in Health Sciences; 2015, 5, 2; 24-29
2083-1617
Pojawia się w:
Progress in Health Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Synthetic peptides mimicking antigenic epitope of Helicobacter pylori urease
Autorzy:
Białek, Magdalena
Grabowski, Sebastian
Kamiński, Zbigniew
Kaca, Wiesław
Powiązania:
https://bibliotekanauki.pl/articles/1041271.pdf
Data publikacji:
2006
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
synthetic peptides
atherosclerosis
urease epitopes
Helicobacter pylori
Opis:
Short peptides resembling the Helicobacter pylori urease antigen (UreB F8 Ser-Ile-Lys-Glu-Asp-Val-Gln-Phe) with deleted aspartic acid and glutamic acid residues, anchored through a triazine linker via the N-terminal moiety to cellulose plate were prepared. The peptides were used for binding of antibodies from sera of patients with medically confirmed atherosclerosis. Recognition of the peptides was also tested with anti-Jack beans urease antibodies. The important role of a Gly-Gly spacer separating the peptides from the cellulose support was shown. Different patterns of binding of antibodies from H. pylori infected patients and anti-Jack bean urease antibodies were observed only in the case of pentapeptides. The peptide Gly-Gly-Leu-Val-Phe-Lys-Thr was recognized by most of the tested sera.
Źródło:
Acta Biochimica Polonica; 2006, 53, 1; 83-86
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Antimicrobial Activity of the Essential oil of Thymus capitatus against Helicobacter pylori
Autorzy:
güvenir, meryem
Hanoglu, Azmi
Hanoglu, Duygu
Suer, Kaya
Baser, Husnu Can
Ozkum Yavuz, Dudu
Sanlıdag, Tamer
Powiązania:
https://bibliotekanauki.pl/articles/895252.pdf
Data publikacji:
2020-02-29
Wydawca:
Polskie Towarzystwo Farmaceutyczne
Tematy:
helicobacter pylori
Thymus capitatus
in vi
essential oil
Opis:
Helicobacter pylori (H.pylori) is an infective agent and the main resourceful factor of chronic, active ot type B gastritis, peptic and duodenal ulcer, gastric carcinoma and mucosa-associated lymphoid tumors. Nowadays, pharmological treatment of H. pylori infections attract attention beside antibiotic treatment. The aim of this study is to analyse the chemical composition of the essential oils of Thymus capitatus and the antimicrobial activity against H. pylori. Aerial parts of wild growing plant materials were collected during flowering stage from three different locations. Essential oil composition analyses were performed simultaneously by GC-FID and GC-MS systems. The antimicrobial activity of the essential oil were tested by broth dilution method. The thymol percentage was determined as more than half of the essential oil composition of Thymus capitatus-Yedidalga (51.9%) and Thymus capitatus –Yıldırım (57.1%); however, the composition of thymol was 47.2% followed by 15.1% (p-cymene), 10.0% (-terpinene) and 5.7% (carvacrol) for Thymus capitatus-Boğaz/Girne.The essential oil from Thymus capitatus- Boğaz/Girne showed the lowest MIC and MBC values and the strongest bactericidal activity against H. pylori compared to the other tested essential oils. Bactericidal efficacy of essential oil of Thymus capitatus on H. pylori could have been affected due to the varied rates of major compounds. Thymus capitatus essential oil could be an alternative way for the treatment of H. pylori infections.
Źródło:
Acta Poloniae Pharmaceutica - Drug Research; 2020, 77, 1; 155-160
0001-6837
2353-5288
Pojawia się w:
Acta Poloniae Pharmaceutica - Drug Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Helicobacter pylori antigens, acetylsalicylic acid, LDL and 7-ketocholesterol - their potential role in destabilizing the gastric epithelial cell barrier. An in vitro model of Kato III cells
Autorzy:
Gajewski, Adrian
Mnich, Eliza
Szymański, Karol
Hinc, Krzysztof
Obuchowski, Michał
Moran, Anthony
Chmiela, Magdalena
Powiązania:
https://bibliotekanauki.pl/articles/1038856.pdf
Data publikacji:
2016
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
Helicobacter pylori
acetylsalicylic acid
7-ketocholesterol
gastric barrier
Opis:
Colonization of gastric tissue in humans by H. pylori Gram-negative bacteria initiates gastric and duodenal ulcers and even gastric cancers. Infections promote inflammation and damage to gastric epithelium which might be followed by the impairment of its barrier function. The role of H. pylori components in these processes has not been specified. H. pylori cytotoxicity may potentially increase in the milieu of anti-inflammatory drugs including acetylsalicylic acid (ASA). The lipid transport-associated molecule such as low density lipoprotein (LDL), which is a classic risk factor of coronary heart disease (CHD) and 7-ketocholesterol (7-kCh) a product of cholesterol oxidation, which may occur during the oxidative stress in LDL could also be considered as pro-inflammatory. The aim of this study was to evaluate the cytotoxicity of H. pylori antigens, ASA, LDL and 7-kCh towards Kato III gastric epithelial cells, on the basis of the cell ability to reduce tetrazolium salt (MTT) and morphology of cell nuclei assessed by 4',6-diamidino-2-phenylindole (DAPI) staining. Kato III cells were stimulated for 24 h, at 37°C and 5% CO2, with H. pylori antigens: cytotoxin associated gene A (CagA) protein, the urease A subunit (UreA), lipopolysaccharide (LPS) and ASA, LDL or 7-kCh. H. pylori LPS, ASA, LDL and 7-kCh, but not H. pylori glycine acid extract (GE), demonstrated cytotoxicity against Kato III cells, which was related to a diminished percentage of MTT reducing cells and to an increased cell population with the signs of DNA damage. The results suggest that damage to gastric epithelial cells can be induced independently by H. pylori antigens, ASA and endogenous lipid transport-associated molecules. During H. pylori infection in vivo, especially in CHD patients, synergistic or antagonistic interactions between these factors might possibly influence the disease course. Further study is necessary to explain these potential effects.
Źródło:
Acta Biochimica Polonica; 2016, 63, 1; 145-152
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Current status of Helicobacter pylori associated human gastric cancer and the therapeutic approaches – A Review
Autorzy:
Pal, Partha
Powiązania:
https://bibliotekanauki.pl/articles/1189965.pdf
Data publikacji:
2016
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:
Helicobacter pylori
Gastric carcinoma
Peptic ulcers
Antibiotic treatment
Opis:
In the recent years one of the common causes of cancer related deaths in humans is associated with the development of gastric carcinoma leading to gastric cancer. It had long before reported that the potent agent that is intimately related with the gastric cancer is the infection of the bacterium Helicobacter pylori (H. pylori). H. pylori infection in humans is characterised by the development of acute peptic ulcers which creates a lots of gastroenterological symptoms. Co-existence of H. pylori in the human gastric environment is well adapted by the pathogen by evading the immune responses of the host. Gastric biopsy in patients suffering from H. pylori infection reported the development of gastric lesions having the chance to proceed towards development gastric cancer. Recently it was reported that eradication of the H. pylori colonization is not possible even after continued current antibiotic treatment over long periods and even combined regimes are unsuccessful.Moreover alterations of the genetic background of the host gastric cells were also reported along with infection of H. pylori, the primary concern for the evolution of the genetically transformed cells which is the hallmark for the development of malignancy. Clinical studies have reported the limited efficacy in decreasing the incidence of gastric carcinoma even after the eradication therapy has been completed for the bacterial colonization. Research based on the biopsy specimens obtained from conventional gastric endoscopy of affected patients has revealed some genetic or epigenetic alterations in the gastric mucosa. The cause of concern to human life lies in the fact that the number of sporadic cases of primary or metachronous gastric carcinoma has increased after successful eradication.The purpose of the present review is to assess the current status of the research based on the concept of development of gastric carcinoma in association with H. pylori infection in humans and to throw some light in the remedial measures against the pathogen which is a chronic burden in the well-being of the human society.
Źródło:
World Scientific News; 2016, 52; 162-180
2392-2192
Pojawia się w:
World Scientific News
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The relative frequency of the Helicobacter pylori Infection in Proximal Gastric Cancers
Autorzy:
Talaiezadeh, Abdolhassan
Hajiani, Eskandar
Tarshizi, Mohammad Ali
Powiązania:
https://bibliotekanauki.pl/articles/1396497.pdf
Data publikacji:
2013-11-01
Wydawca:
Index Copernicus International
Tematy:
distal gastric cancer
Helicobacter pylori
proximal gastric cancer
Opis:
Gastric cancer with the annual mortality of 700 000 is the second cause of death in the world. Iran with the annual incidence of 37 in 100 000 is among areas with high risk of gastric cancer. In Iran, Khuzestan Province with the annual incidence of 10 -15 in 100 000 is among areas with moderate risk. The role of Helicobacter pylori infection in distal gastric cancer as one of the most important risk factors of gastric ulcer has been proved but its role in proximal gastric cancer is controversial. In recent decades, the incidence of proximal cancers has increased. The aim of the study was to survey the relative frequency of Helicobacter pylori infection in the people with proximal cancer and to compare it with this infection frequency in the distal cancer. Material and methods. Relative frequency of Helicobacter pylori infection was surveyed by pathology tests and biopsy gastric mucosa sample staining with H&E in the patients with proximal and distal gastric ulcer whose cancers were proved by pathology method and also in the people who were healthy in terms of a gastric cancer. Data were statistically analyzed by frequency tables and chi square test. Results. One hundred and seventeen people entered the study. Among 32 patients with the proximal cancer, 21 people were with the positive Helicobacter pylori (65.6%) and 11 people (34.4%) were with the negative Helicobacter pylori (p<0.01). Among 30 patients with the distal cancer, 26 people were with positive Helicobacter pylori (86.7%) and four people were with negative Helicobacter pylori (13.3%) (p<0.01); 55 people were without the cancer, which among them, 40 people were with the positive Helicobacter pylori (72.7%); and 15 people were with the negative Helicobacter pylori (37.7%) (p<0.01). In comparison of the frequency of Helicobacter pylori infection in the patients with the proximal cancer, a weak significant difference exists (p = 0.053). Comparing the frequency of Helicobacter pylori infection in the patients with the gastric cancer and people without the cancer, no significant difference existed (p = 0.703). Conclusion. A weak significant difference existed between the frequency of Helicobacter pylori infection in patients with the proximal gastric cancer and the frequency of these infections in the patients with the distal cancer. So, eradicating Helicobacter pylori infection can be effective in preventing the distal gastric cancers in addition to preventing the proximal gastric cancers.
Źródło:
Polish Journal of Surgery; 2013, 85, 11; 657-662
0032-373X
2299-2847
Pojawia się w:
Polish Journal of Surgery
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
PCR-RFLP detection of point mutations A2143G and A2142G in 23S rRNA gene conferring resistance to clarithromycin in Helicobacter pylori strains
Autorzy:
Klesiewicz, Karolina
Nowak, Paweł
Karczewska, Elżbieta
Skiba, Iwona
Wojtas-Bonior, Izabela
Sito, Edward
Budak, Alicja
Powiązania:
https://bibliotekanauki.pl/articles/1039295.pdf
Data publikacji:
2014
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
Helicobacter pylori
clarithromycin resistance
PCR-RFLP
point mutations
Opis:
Background. The occurrence of clarithromycin resistance among Helicobacter pylori strains is a major cause of the treatment failure. Resistance to this drug is conferred by point mutations in 23S rRNA gene and the most prevalent mutations are A2143G and A2142G. The aim of the study was to evaluate the occurrence of A2143G and A2142G mutations in a group of H. pylori strains resistant to clarithromycin. Materials and Methods. The study included 21 clarithromycin-resistant H. pylori strains collected between 2006 and 2009 in southern Poland. Resistance to clarithromycin was quantitatively tested with the E-test to determine the minimal inhibitory concentration (MIC value). The point mutations of H. pylori isolates were detected by PCR followed by RFLP analysis. Results. The MIC values for clarithromycin for the analyzed strains ranged from 1.5 mg/L to 64 mg/L. Nine H. pylori strains exhibited A2143G mutation and A2142G mutation was found in 9 isolates as well. The results of RFLP analysis of 3 clarithromycin-resistant strains were negative for both mutations. The average MIC values for A2143G and A2142G mutants were 6 and 30 mg/L, respectively. Conclusions. Frequencies of A2143G and A2142G mutations were the same in all isolates tested. Strains with A2143G mutation exhibited lower MIC values than A2142G mutants. Application of PCR-RFLP method for detection of clarithromycin resistance allows for better and more efficient management of H. pylori infections.
Źródło:
Acta Biochimica Polonica; 2014, 61, 2; 311-315
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Genetic immunization of ducks for production of antibodies specific to Helicobacter pylori UreB in egg yolks.
Autorzy:
Kazimierczuk, Kacper
Cova, Lucyna
Ndeboko, Benedicte
Szczyrk, Urszula
Targosz, Aneta
Brzozowski, Tomasz
Sirko, Agnieszka
Powiązania:
https://bibliotekanauki.pl/articles/1041486.pdf
Data publikacji:
2005
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
urease
antibody
antigen
IgY
DNA immunization
Helicobacter pylori
Opis:
Following genetic immunization of laying ducks with a plasmid expressing Helicobacter pylori UreB (large subunit of urease), IgY against UreB were obtained from egg yolks. These polyclonal and monospecific IgY antibodies are of higher-titer and specifically recognize recombinant H. pylori urease purified from Escherichia coli. To our knowledge this is the first report describing generation of IgY antibodies directed against antigens of H. pylori by DNA-based immunization.
Źródło:
Acta Biochimica Polonica; 2005, 52, 1; 261-266
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-15 z 54

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