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Tytuł:
Occupational risk assessment of oxidative stress and genotoxicity in workers exposed to paints during a working week
Autorzy:
Cassini, Carina
Calloni, Caroline
Bortolini, Giovana
Garcia, Solange C.
Dornelles, Marco A.
Henriques, João A.
Erdtmann, Bernardo
Salvador, Mirian
Powiązania:
https://bibliotekanauki.pl/articles/2185389.pdf
Data publikacji:
2011-09-01
Wydawca:
Instytut Medycyny Pracy im. prof. dra Jerzego Nofera w Łodzi
Tematy:
paint exposure
Oxidative stress
genotoxicity
Opis:
Objectives: Paints are complex mixtures of solvents and metals that can induce health damages in workers exposed to them. The aim of the present work was to evaluate possible oxidative and genotoxic effects in workers exposed to paints. Material and Methods: Peripheral blood and buccal cell samples were collected from 33 workers exposed to paints and 29 non-exposed workers (controls) during an ordinary working week (Monday morning and Friday evening). Oxidative markers were assessed using thiobarbituric acid assay, carbonylated proteins, superoxide dismutase and catalase activities. Hippuric acid and delta-aminolevulinic acid were determined as biomarkers of toluene and lead exposure, respectively. Genotoxicity was measured through comet assay and micronucleus (MN) frequencies. Results: The exposed group showed higher hippuric acid and delta-aminolevulinic acid levels (Friday samples) and lower superoxide dismutase activity (Monday samples) in relation to control group. DNA damage index (comet assay) was higher in the exposed group, both in Monday and Friday samples, compared to the control group. No differences were observed in frequency of micronuclei (MN) between the groups, either in lymphocytes or buccal cells. However, the exposed group presented an increase (Monday samples) in nuclear buds frequency in lymphocytes — a marker of gene amplification — as well as an increase in condensed chromatin in the buccal cells (Monday and Friday samples), suggesting induction of apoptosis. Furthermore, a decrease in the nuclear division index (Friday samples) was observed in the exposed group, indicating that paint exposure induces cytostatic effects in lymphocytes. Conclusion: The results suggest that individuals exposed to paints have increased levels of DNA damage.
Źródło:
International Journal of Occupational Medicine and Environmental Health; 2011, 24, 3; 308-319
1232-1087
1896-494X
Pojawia się w:
International Journal of Occupational Medicine and Environmental Health
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
A preliminary assessment of singlet oxygen scavenging, cytotoxic and genotoxic properties of Geranium macrorrhizum extracts
Autorzy:
Venskutonis, Petras
Dedonytė, Veronika
Lazutka, Juozas
Slapšytė, Gražina
Marozienė, Audronė
Nemeikaitė-Čėnienė, Aušra
Čėnas, Narimantas
Miliauskas, Giedrius
Powiązania:
https://bibliotekanauki.pl/articles/1040398.pdf
Data publikacji:
2010
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
cytotoxicity
Geranium macrorrhizum
polyphenols
genotoxicity
Opis:
Strong radical-scavenging activity of Geranium macrorrhizum extracts isolated by using various solvent systems has been reported previously. This study aimed at expanding the knowledge on the bioactivities of antioxidatively active G. macrorrhizum butanol fraction, which was isolated from ethanolic extract (EB), and water fraction, which was isolated from water extract (WW) by measuring their singlet oxygen scavenging properties, as well as preliminary assessment of cytotoxicity and genotoxicity toward mammalian cells. The cytotoxicity (necrosis induction) of the extracts in bovine leukemia virus-transformed lamb kidney fibroblasts (line FLK) was partly prevented by antioxidants and stimulated by the prooxidant BCNU (N,N'-bis(2-chloroethyl)-N-nitrosourea). This indicates that the cytotoxicity of G. macrorrhizum extracts is at least partly attributed to their prooxidant action, presumably due to the formation of quinoidal products of their (auto)oxidation. The latter was evidenced by the nature of the peroxidase-catalyzed oxidation products, which supported DT-diaphorase-catalyzed oxidation of NADPH and participated in conjugation reactions with reduced glutathione. The genotoxic properties were studied using chromosome aberration (CA) and sister chromatid exchange (SCE) tests in human lymphocytes in vitro and Drosophila melanogaster somatic mutation and recombination test (SMART) in vivo. In the CA test, only the highest doses of both fractions significantly increased chromosome aberration frequency. In the SCE test, both fractions induced SCEs in a clear dose-dependent manner. G. macrorrhizum extracts were not genotoxic in the SMART test in vivo. Our data indicate that in spite of the possible beneficial (antioxidant) effects of Geranium extracts, the possibilities of their use as ingredients of functional foods and/or food supplements should be further examined due to their cyto- and genotoxic effects resulting mainly from the action of quercetin-derived components abundant in the extracts.
Źródło:
Acta Biochimica Polonica; 2010, 57, 2; 157-163
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Probiotic preparation reduces faecal water genotoxicity and cytotoxicity in chickens fed ochratoxin A contaminated feed (in vivo study)
Autorzy:
Śliżewska, Katarzyna
Nowak, Adriana
Smulikowska, Stefania
Powiązania:
https://bibliotekanauki.pl/articles/1038813.pdf
Data publikacji:
2016
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
ochratoxin A
probiotics
chicken
genotoxicity
cytotoxicity
Opis:
The aim of the present study was to assess the genotoxicity and cytotoxicity of the faecal water of chickens fed ochratoxin A (OTA) contaminated feed with and without probiotic preparation. The study was performed on 20 healthy female Ross broiler chickens divided into 4 groups: control chickens - fed with non-supplemented feed; PP chickens - fed feed supplemented with the probiotic preparation; OTA chickens - fed feed contaminated with 1 mg per kg of OTA; OTA + PP chickens - fed feed contaminated with 1 mg per kg of OTA and supplemented with the probiotic preparation. Faecal water samples were collected on the 35th day of life of chickens from each group. Genotoxicity was measured using the comet assay, and cytotoxicity by means of MTT tests. Mean DNA damage, measured as the percentage of DNA in the tails of the comets, was 8.50 ± 1.10 for chickens fed OTA at 1 mg/kg and 6.41 ± 0.67 in the controls. The supplementation of feed with the probiotic preparation decreased the extent of DNA damage to 4.74 ± 0.78. In the control group of chickens the average cytotoxicity was 38.5 ± 0.5 (in MTT), while in the probiotic preparation group (PP group) it was 31.8 ± 0.7 (in MTT). After supplementation of the feed with the probiotic preparation, the genotoxicity and cytotoxicity were decreased in a statistically significant manner.
Źródło:
Acta Biochimica Polonica; 2016, 63, 2; 281-286
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Use of Escherichia Coli K-12 Strain With Gfp Reporter Gene for Screening of Cefuroxime and Metronidazole Residues in Surface Water
Autorzy:
Matejczyk, M.
Rosochacki, S. J.
Jabłonowski, K.
Powiązania:
https://bibliotekanauki.pl/articles/123587.pdf
Data publikacji:
2015
Wydawca:
Polskie Towarzystwo Inżynierii Ekologicznej
Tematy:
recA
gfp biosensor
cytotoxicity
genotoxicity
cefuroxime
metronidazole
Opis:
Antibiotic residues are constantly detected in environmental waters at relatively low concentrations. They can modulate the biological stability of ecosystems after entering the environment, therefore robust and reliable methods for determining their residues in environmental samples are required. In this work, Escherichia coli K-12 GFP-based bacterial biosensors strain was used to detect cyto- and genotoxic activity of cefuroxime and metronidazole at concentration of 500, 1000, 2000, 3000 and 5000 μg/ml. Experimental data indicated that metronidazole, at higher concentrations of 3000 and 5000 μg/ml showed stronger cyto- and genotoxix activity than cefuroxime to bacteria cells. Incubation of bacteria cells with surface water with both drugs modulated gfp gene expression. E. coli K-12 strain with genetic fusion of recA promoter and gfp reporter gene was a good model organism for screening cyto- and genotoxic effect of cefuroxime and metronidazole in applied in this experiment concentrations of these drugs.
Źródło:
Journal of Ecological Engineering; 2015, 16, 3; 77-82
2299-8993
Pojawia się w:
Journal of Ecological Engineering
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Potential applications of SOS-GFP biosensor to in vitro rapid screening of cytotoxic and genotoxic effect of anticancer and antidiabetic pharmacist residues in surface water
Autorzy:
Matejczyk, M.
Rosochacki, S. J.
Powiązania:
https://bibliotekanauki.pl/articles/124844.pdf
Data publikacji:
2015
Wydawca:
Polskie Towarzystwo Inżynierii Ekologicznej
Tematy:
SOS-gfp biosensor
cytotoxicity
genotoxicity
cyclophosphamide
metformin
Opis:
Escherichia coli K-12 GFP-based bacterial biosensors allowed the detection of cytotoxic and genotoxic effect of anticancer drug– cyclophosphamide and antidiabetic drug – metformin in PBS buffer and surface water. Experimental data indicated that recA::gfpmut2 genetic system was sensitive to drugs and drugs mixture applied in experiment. RecA promoter was a good bioindicator in cytotoxic and genotoxic effect screening of cyclophosphamide, metformin and the mixture of the both drugs in PBS buffer and surface water. The results indicated that E. coli K-12 recA::gfp mut2 strain could be potentially useful for first-step screening of cytotoxic and genotoxic effect of anticancer and antidiabetic pharmacist residues in water. Next steps in research will include more experimental analysis to validate recA::gfpmut2 genetic system in E. coli K-12 on different anticancer drugs.
Źródło:
Journal of Ecological Engineering; 2015, 16, 1; 116-121
2299-8993
Pojawia się w:
Journal of Ecological Engineering
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
A conjugate of pyridine-4-aldoxime and atropine as a potential antidote against organophosphorus compounds poisoning
Autorzy:
Lovrić, Jasna
Berend, Suzana
Lucić Vrdoljak, Ana
Radić, Božica
Katalinić, Maja
Kovarik, Zrinka
Želježić, Davor
Kopjar, Nevenka
Rast, Slavko
Mesić, Milan
Powiązania:
https://bibliotekanauki.pl/articles/1039913.pdf
Data publikacji:
2011
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
antidotal potential
atropine
genotoxicity
pyridine-4-aldoxime
organophosphates
Opis:
A conjugate of pyridine-4-aldoxime and atropine (ATR-4-OX) was synthesized and its antidotal efficiency was tested in vitro on tabun- or paraoxon-inhibited acetylcholinesterase (AChE) of human erythrocytes as well as in vivo using soman-, tabun- or paraoxon-poisoned mice. Its genotoxic profile was assessed on human lymphocytes in vitro and was found acceptable for further research. ATR-4-OX showed very weak antidotal activity, inadequate for soman or tabun poisoning. Conversely, it was effective against paraoxon poisoning both in vitro and in vivo. All animals treated with 5 % or 25 % LD50 doses of the new oxime survived after administration of 10.0 or 16.0 LD50 doses of paraoxon, respectively. Based on the persistence of toxicity symptoms in mice, the atropine moiety had questionable effects in attenuating such symptoms. It appears that ATR-4-OX has a therapeutic effect related to the reactivation of phosphylated AChE, but not to receptor antagonization.
Źródło:
Acta Biochimica Polonica; 2011, 58, 2; 193-198
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Evaluation of biological effects of nanomaterials. Part I. Cyto- and genotoxicity of nanosilver composites applied in textile technologies
Autorzy:
Wąsowicz, Wojciech
Cieślak, Małgorzata
Palus, Jadwiga
Stańczyk, Małgorzata
Dziubałtowska, Elżbieta
Stępnik, Maciej
Düchler, Markus
Powiązania:
https://bibliotekanauki.pl/articles/2185373.pdf
Data publikacji:
2011-12-01
Wydawca:
Instytut Medycyny Pracy im. prof. dra Jerzego Nofera w Łodzi
Tematy:
nanosilver composites
cytogenotoxicity
genotoxicity
DNA damage
textile technology
Opis:
Objectives: The aim of this study was to investigate the cyto- and genotoxicity of nanocomposites (NCs) and generation of reactive oxygen species (ROS) as a result of particle-cell interactions. Materials and Methods: Titanium dioxide (TiO₂-Ag) and ion-exchange resin (Res-Ag), both coated with silver (Ag), were examined. The murine macrophage J774A.1 cells were incubated in vitro with NC at different concentrations for 24 h. Cytotoxicity was analyzed by the methylthiazolyldiphenyltetrazolium bromide reduction test (MTT reduction test). ROS generation was assessed by incubation of cells with dichlorodihydrofl uorescein diacetate (DCF) and fl ow cytometry. DNA damage was detected by comet assay and included single-strand breaks (SSB), alkali-labile sites (ALS) and oxidative DNA damage after formamidopyrimidine glycosylase (FPG) treatment. The tail moment was used as an indicator of DNA damage. Results: TiO₂-Ag was not cytotoxic up to 200 μg/ml, whereas IC₅₀ for Res-Ag was found to be 23 μg/ml. Intracellular ROS levels were elevated after 4 h of exposure to Res-Ag at the concentration of 50 μg/ml. Both types of NC induced fragmentation of DNA strands, but only one of the composites caused damage to purine bases. TiO₂-Ag induced SSB of DNA at concentrations of 10 and 5 μg/ml. For Res-Ag, a concentration-dependent increase in tail moments was observed. Conclusions: Silver-coated nanocomposites (both TiO₂- Ag and Res-Ag) may cause genotoxic effects in murine macrophages J774A.1. Res-Ag increased generation of ROS which suggested that toxicity of Res-Ag in murine macrophages is likely to be mediated through oxidative stress. This paper will support industry and regulators alike in the assessment of hazards and risks and methods for their mitigation at the earliest possible stage in material and product development.
Źródło:
International Journal of Occupational Medicine and Environmental Health; 2011, 24, 4; 348-358
1232-1087
1896-494X
Pojawia się w:
International Journal of Occupational Medicine and Environmental Health
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Dose- and time-dependent micronucleus induction in peripheral erythrocytes of catfish, Heteropneustes fossilis (Bloch) by zinc
Autorzy:
Talapatra, S.N.
Banerjee, P.
Mukhopadhyay, A.
Powiązania:
https://bibliotekanauki.pl/articles/11433.pdf
Data publikacji:
2014
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:
micronucleus
peripheral blood
erythrocyte
catfish
Heteropneustes fossilis
zinc
genotoxicity
Opis:
As far as the detection of metal genotoxicity in fish is concerned, micronucleus (MN) test is considered an extremely suitable measure. In this study, frequencies of micronucleated erythrocytes were scored in peripheral blood of catfish, Heteropneustes fossilis (bloch) after acute in-vivo exposure of zinc at different concentrations (5, 10 and 30 ppm) in the laboratory condition. These three concentrations of zinc were tested at different durations such as 24h, 48h, 72h and 96h respectively. Highly significant (P < 0.001) increased values were obtained for MN frequencies in the peripheral erythrocytes of exposed fishes compared to control groups of fishes. These results confirm that dose- and time-dependent micronucleation in the peripheral erythrocytes of fish after short-term exposure to zinc could provide valuable information regarding zinc containing effluent quality and also help in genetic biomonitoring with this test model. In this context safe concentration of zinc vis-a-vis genotoxicity range could be evaluated for future studies.
Źródło:
International Letters of Natural Sciences; 2014, 04
2300-9675
Pojawia się w:
International Letters of Natural Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Escherichia Coli-Lux Biosensor Used to Monitor the Cytotoxicity and Genotoxicity of Pharmacological Residues in Environment
Autorzy:
Hawrylik, E.
Matejczyk, M.
Powiązania:
https://bibliotekanauki.pl/articles/124989.pdf
Data publikacji:
2018
Wydawca:
Polskie Towarzystwo Inżynierii Ekologicznej
Tematy:
bacterial biosensors
reporter gene lux
β-blockers
cytotoxicity
genotoxicity
Opis:
The aim of the study was to evaluate the usefulness of Escherichia coli K-12 RFM 443 recA::lux for cytotoxicity and genotoxicity monitoring of metoprolol in the environment. Metoprolol is one of the most popular cardiac drug which belongs to the group of β-blockers. The drug was applied at the concentrations ranging from 0.01 μg/cm3 to 100 μg/cm3. The conducted research constitutes preliminary study aimed at validation of the recA::lux gene construct in order to determine its sensitivity to metoprolol. The drug concentrations were selected experimentally to obtain a positive luminescence response. The obtained data indicated the influence of metoprolol on lux gene expression and recA promoter activity based on the application of laboratory samples using PBS buffer. The results indicate a potential for using a bacterial biosensor Escherichia coli K-12 RFM 443 with recA::lux gene fusion in cytotoxicity and genotoxicity monitoring of the cardiac drugs residue in the environment.
Źródło:
Journal of Ecological Engineering; 2018, 19, 3; 11-17
2299-8993
Pojawia się w:
Journal of Ecological Engineering
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Specific for DNA damages GFP microbial biosensor as a tool for genotoxic action assessment of environmental pollution
Autorzy:
Matejczyk, M.
Powiązania:
https://bibliotekanauki.pl/articles/403239.pdf
Data publikacji:
2010
Wydawca:
Politechnika Białostocka. Oficyna Wydawnicza Politechniki Białostockiej
Tematy:
uszkodzenia DNA
genotoksyczność
DNA damage
genotoxicity
recA promoter
SOS response
Opis:
In the presented paper, autofluorescent reporter of Escherichia coli K-12 recA::gfpmut2 strain, which contained a plasmid-borne transcriptional fusion between DNA-damage inducible recA promoter involved in the SOS regulon response and fast folding GFP variant reporter gene-gfpmut2, have been used. GFP-based bacterial biosensors allowed the detection of bacterial cells response to selected tested genotoxic compounds such as mitomycin C (MMC), actinomycin D, N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) and formaldehyde (CH2O). Experiment indicated that E. coli K-12 recA::gfpmut2 biosensor strain is more specific and sensitive for especially two genotoxins: actinomycin D and MNNG and with very low response to other agents. So it was concluded that for formaldehyde and MMC E. coli K-12 recA::gfpmut2 genetic system is disqualified for genotoxicity screening.
Źródło:
Budownictwo i Inżynieria Środowiska; 2010, 1, 4; 319-326
2081-3279
Pojawia się w:
Budownictwo i Inżynieria Środowiska
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Genotoxicity detection with special reference to micronucleation in the erythrocytes of fish species due to water pollution - a mini review
Autorzy:
Talapatra, S.N.
Nandy, A.
Powiązania:
https://bibliotekanauki.pl/articles/11317.pdf
Data publikacji:
2014
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:
genotoxicity
detection
micronucleation
erythrocyte
fish species
water pollution
aquatic ecosystem
Opis:
Water pollution is a matter of great concern in lentic and lotic ecosystems. Generally water pollutants like heavy metals, organic compounds, etc. generate from industries and domestic activities. All of these pose serious threat to the fish population in freshwater as well as marine water bodies. An impact caused by water pollutants especially genotoxins depend not only upon its concentration, but also on the duration (acute and chronic exposure) and showed individual and/or combination of pollutants or genotoxins. Genotoxicity of fish with special reference to micronucleus induction in the erythrocytes of fish is an easy screening of water pollution. The present review deals with past and present research works of major water pollutants discharge and their impact on several fish species from available literatures.
Źródło:
International Letters of Natural Sciences; 2014, 12, 1
2300-9675
Pojawia się w:
International Letters of Natural Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Tenocyclidine treatment in soman-poisoned rats - intriguing results on genotoxicity versus protection
Autorzy:
Petek, Maja
Berend, Suzana
Kopjar, Nevenka
Želježić, Davor
Mladinić, Marin
Radić, Božica
Vrdoljak, Ana
Powiązania:
https://bibliotekanauki.pl/articles/1040822.pdf
Data publikacji:
2008
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
rat
tenocyclidine
soman
cholinesterase activity
brain
genotoxicity
comet assay
plasma
Opis:
This study aimed to evaluate the antidotal potency of tenocyclidine (TCP) that probably might protect acetylcholinesterase (AChE) in the case of organophosphate poisoning. TCP was tested alone as a pretreatment or in combination with atropine as a therapy in rats poisoned with ¼ and ½ of LD50 of soman. Possible genotoxic effects of TCP in white blood cells and brain tissue were also studied. Results were compared with previous findings on the adamantyl tenocyclidine derivative TAMORF. TCP given alone as pretreatment, 5 min before soman, seems to be superior in the protection of cholinesterase (ChE) catalytic activity in the plasma than in brain, especially after administration of the lower dose of soman. Plasma activities of the enzyme after a joint treatment with TCP and soman were significantly increased at 30 min (P < 0.001) and 24 h (P = 0.0043), as compared to soman alone. TCP and atropine, given as therapy, were more effective than TCP administered alone as a pretreatment. The above therapy significantly increased activities of the enzyme at 30 min (P = 0.046) and 24 h (P < 0.001), as compared to controls treated with ¼ LD50 of soman alone. Using the alkaline comet assay, acceptable genotoxicity of TCP was observed. However, the controversial role of TCP in brain protection of soman-poisoned rats should be studied further.
Źródło:
Acta Biochimica Polonica; 2008, 55, 1; 97-106
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Evaluation of Selected Toxicity Endpoints in Ovary Cells Exposed to Nanoceria
Ocena wybranych parametrów toksycznego działania nanocząstek ditlenku ceru na komórki CHO-9
Autorzy:
Zapór, L.
Powiązania:
https://bibliotekanauki.pl/articles/1818019.pdf
Data publikacji:
2016
Wydawca:
Politechnika Koszalińska. Wydawnictwo Uczelniane
Tematy:
cerium dioxide nanoparticles
nanoceria
cytotoxicity
genotoxicity
nanocząstki ditlenku ceru
cytotoksyczność
genotoksyczność
Opis:
Nanometryczny tlenek ceru (CeO2NM) ma szerokie zastosowanie w wielu sektorach przemysłu, co stwarza możliwość emisji do środowiska (zarówno środowiska naturalnego, jak i środowiska pracy). Stosowany jest przede wszystkim jako katalizator paliwa w silnikach Diesla, elektrolit w stało tlenkowych ogniwach paliwowych (SOFC, solid oxide fuel cells), materiał polerski, barwnik plastiku, warstwa buforowa dla nadprzewodników, powłoka dla filtrów podczerwieni, pochłaniacz UV oraz jako przeciwutleniacz w biomedycynie. Pomimo, że produkcja CeO2NM jest wielkotonażowa, to istniejące dane toksykologiczne, z uwagi na niespójny obraz toksycznego działania, uznane zostały za niewystarczające do oceny zagrożeń, jakie może stwarzać dla ludzi i środowiska. Celem badań była ocena toksycznego działania CeO2NM o nominalnej wielkości cząstek: < 25 nm na komórki układu rozrodczego (CHO-9), po krótkotrwałym (24 lub 72 h) i długotrwałym (7 dni) narażeniu komórek. Badano wpływ CeO2NM na integralność błon komórkowych (test NRU), aktywność metaboliczną komórek (test MTT, test WST-1) oraz zdolność komórek do proliferacji (test wydajności tworzenia kolonii - CFEA). Oceniono również wpływ CeO2NM na uszkodzenia DNA w komórkach poprzez oznaczenie metodą kometową (SCGE w warunkach zasadowych) jednoniciowych pęknięć DNA (SSB) oraz oksydacyjnych uszkodzeń zasad rozpoznawanych przez glikozylazę formamido-pirymidynową (SSB-FPG). CeO2NM powodował zależne od stężenia i czasu narażenia działanie cytotoksyczne. Kierunek zmian toksycznych obejmował zarówno zmiany w przepuszczalności błon komórkowych, jak i zaburzenia aktywności metabolicznej mitochondriów. Zakres stężeń cytotoksycznych wynosił 100-400 µg/ml zależnie od ocenianego skutku cytotoksycznego. Długotrwałe narażenie komórek na CeO2NM powodowało utratę zdolności komórek do proliferacji w stężeniach ok. 50 µg/ml. CeO2NM powodował zależny od stężenia istotny statystycznie wzrost poziomu jednoniciowych pęknięć DNA oraz oksydacyjnych uszkodzeń DNA rozpoznawanych przez FPG w narażanych komórkach już w najniższych ze stosowanych stężeń (10-100 µg/ml). Wyniki testu kometowego i klonogennego wskazują na potencjalną toksyczność przewlekłą CeO2NM.
Źródło:
Rocznik Ochrona Środowiska; 2016, Tom 18, cz. 2; 389-403
1506-218X
Pojawia się w:
Rocznik Ochrona Środowiska
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Relationship between DNA replication and DNA repair in human lymphocytes proliferating in vitro in the presence and in absence of mutagen
Autorzy:
Szyfter, K
Wiktorowicz, K.
Wielgosz, M.S.
Zajaczek, S.
Kujawski, M.
Jaloszynski, P.
Czub, M.
Markowska, J.
Powiązania:
https://bibliotekanauki.pl/articles/2048210.pdf
Data publikacji:
1995
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
genotoxicity
DNA replication
human lymphocyte
DNA repair
lymphocyte proliferation
mutagenesis
in vitro
Opis:
The effects of mutagens on DNA replication and DNA repair were studied in peripheral blood lymphocytes (PBL) obtained from 21 healthy subjects, 2 samples from healthy heterozygote of Xeroderma pigmentosum (XP) and 2 samples from patient with clinically recognised XP. Inter-individual variations were found in DNA replication and in the level of spontaneous DNA repair measured under standard culture condition. Exposure of human PBL proliferating in vitro to B(a)P was followed by a partial inhibition of replicative DNA synthesis in all subjects and by an induction of DNA repair in healthy subjects. In XP patients DNA repair synthesis remained at the level attributed to spontaneous DNA repair. The response to mutagen varied individually. Results were analysed statistically. It was established that the studied indices of DNA synthesis correlate well with each other. The highest correlation was found between the levels of spontaneous and B(a)P-induced DNA repair. It is concluded that the level of spontaneous DNA repair is predictive for an estimation of cells ability to repair DNA damage. Inter-individual variations in the inhibition of DNA replication and in DNA repair synthesis are also dependent on the type of mutagen as shown by effects of other mutagens. Different effects of mutagen exposure on the inhibition of DNA replicative synthesis and induction of DNA repair can be explained by genetically controlled differences in the activity of enzymes responsible for mutagen processing and lesion removal.
Źródło:
Journal of Applied Genetics; 1995, 36, 4; 379-388
1234-1983
Pojawia się w:
Journal of Applied Genetics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Cytotoxicity and genotoxicity of GO-Fe3O4 hybrid in cultured mammalian cells
Autorzy:
Jedrzejczak-Silicka, M.
Powiązania:
https://bibliotekanauki.pl/articles/779480.pdf
Data publikacji:
2017
Wydawca:
Zachodniopomorski Uniwersytet Technologiczny w Szczecinie. Wydawnictwo Uczelniane ZUT w Szczecinie
Tematy:
GO-Fe3O4 hybrid
relative viability
DNA fragmentation
micronucleus assay
genotoxicity
Opis:
The study was aimed at investigating the effect of the Fe3O4  hybrid deposited on graphene oxide (GO- Fe3O4) on the relative viability and DNA integrity. The properties of the GO-Fe3O4  hybrid were analyzed using a transmission electron microscopy (TEM), X-ray diffraction technique (XRD) and thermal gravimetric method (TGA), while the efficiency of graphene oxide covalent functionalization with iron oxide nanospheres was determined by Fourier transform infrared spectroscopy (FT-IR). L929 and MCF-7 cell lines were selected to analyze the biocompatibility of GO-Fe3O4  nanoparticles. The hybrid was tested using WST-1 and LDH leakage assays. DNA integrity was analyzed by agarose gel electrophoresis and micronucleus assay was performed to examine chromosomal damage in the exposed cell lines. The tested GO-Fe3O4  hybrid did not significantly reduce cell metabolism of L929 cells. GO-Fe3O4 hybrid particles only slightly affected the integrity of cell membranes. DNA integrity and micronucleus assays did not indicate genotoxicity of the hybrid.
Źródło:
Polish Journal of Chemical Technology; 2017, 19, 1; 27-33
1509-8117
1899-4741
Pojawia się w:
Polish Journal of Chemical Technology
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-15 z 23

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