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    Wyświetlanie 1-9 z 9
    Tytuł:
    Protection of visual data transmission for vessel traffic systems using Joint Fingerprinting and Decryption method based on modified hill cipher
    Autorzy:
    Czaplewski, B.
    Czaplewski, K.
    Powiązania:
    https://bibliotekanauki.pl/articles/320701.pdf
    Data publikacji:
    2012
    Wydawca:
    Polskie Forum Nawigacyjne
    Tematy:
    data protecting
    VTS
    fingerprinting
    Hill cipher
    Opis:
    Vessel traffic systems provide a high level of safety on coastal waters due to coastal radar stations and industrial cameras transmitting information to traffic supervision centers, as well. To improve a vessel traffic services is very important to ensure the speed and secrecy for the transmission of video images. The paper presents the basic issues of the multimedia data protection by digital watermarking and fingerprinting methods. Main applications for such digital marking were described in the paper as well as its requirements. Furthermore, the importance of multicast transmission for fingerprinting methods was presented by comparing the scalability of methods using only unicast transmissions and methods using multicast transmissions. The paper also presents the greatest threat to fingerprinting methods, which are attacks performed by more than one pirate. These attacks are called collusion attacks. The criteria that should be followed during identifying rogue users taking part in the collusion attack on the security systems has been presented. The paper also contains description of the extended Hillcast method, which belongs to the group of JFD (Joint Fingerprinting and Decryption) methods. The method provides a cryptographic security and digital fingerprinting of multimedia content, while maintaining high scalability. Main purpose of this method is VoD (Video on Demand) service, but it can also be used in vessel traffic supervision systems, such as VTS and AIS. In the last part of paper, there are results of studies which indicate high resistance to most common collusion attacks. Method proposed by authors can enhance the security of visual data transmission in vessel traffic systems.
    Źródło:
    Annual of Navigation; 2012, No. 19, part 2; 5-17
    1640-8632
    Pojawia się w:
    Annual of Navigation
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Spectral fingerprinting revealed modulation of plant secondary metabolites in providing abiotic stress tolerance to invasive alien plants Lantana camara (L.), Parthenium hysterophorous (L.), Ricinus communis (L.), and Ageratum conyzoides (L.) (plant metabolites in stress tolerance to invasive plants)
    Autorzy:
    Sharma, A.D.
    Kaur, J.
    Chand, T.S.P.
    Powiązania:
    https://bibliotekanauki.pl/articles/2096781.pdf
    Data publikacji:
    2021
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    chemical fingerprinting
    invasiveness
    lipid peroxidation
    secondary metabolites
    Opis:
    Invasive alien species are non-native plant species that displace native species and pose adverse effects to environment, ecosystem, economy, and human health by diminishing the growth of native plants and by exhibiting higher stress tolerance. In our present study, four invasive alien species, namely Lantana camara, Parthenium hysterophorous, Ricinus communis, and Ageratum conyzoides, were studied from different locations. Plants growing under natural environmental conditions were sampled at random in the vicinity of Jalandhar. To gain insights into the biochemical basis of invasiveness of these plants, the samples were subjected to chemical fingerprinting by using UV-Vis, fluorescent, and Fourier transform-infrared (FT-IR) techniques under natural abiotic stress conditions (moderate and hot conditions). Indices of oxidative stress, such as malondialdehyde (MDA), were also studied. MDA levels were enhanced under hot conditions. Elevated peaks (major and minor) were observed in UV fingerprinting during adverse abiotic conditions. Fluorescent spectroscopy also validated the enhanced levels of secondary metabolites. FT-IR spectroscopy confirmed the presence of alkaloids and phenolics during stress conditions. Peaks were identified as rutin, vanillic acid, ascorbic acid, and glutathione reduced. The obtained results showed that under stressful conditions, the studied plants may produce an increased level of metabolites that might play a role in minimizing the oxidative stress faced by these plants. It was concluded that the studied plants, namely P. hysterophorus, L. camara, R. communis, and A. conyzoides, have the potential to cope with abiotic stress such as high temperature, which could be the reason for their invasiveness and vast adaptability.
    Źródło:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2021, 102, 3; 307-319
    0860-7796
    Pojawia się w:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Application of molecular techniques for the assessment of microorganism diversity on cultural heritage objects
    Autorzy:
    Otlewska, Anna
    Adamiak, Justyna
    Gutarowska, Beata
    Powiązania:
    https://bibliotekanauki.pl/articles/1039279.pdf
    Data publikacji:
    2014
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    molecular techniques
    microbial diversity
    metagenomics
    genetic fingerprinting
    cultural heritage
    biodeterioration
    Opis:
    As a result of their unpredictable ability to adapt to varying environmental conditions, microorganisms inhabit different types of biological niches on Earth. Owing to the key role of microorganisms in many biogeochemical processes, trends in modern microbiology emphasize the need to know and understand the structure and function of complex microbial communities. This is particularly important if the strategy relates to microbial communities that cause biodeterioration of materials that constitute our cultural heritage. Until recently, the detection and identification of microorganisms inhabiting objects of cultural value was based only on cultivation-dependent methods. In spite of many advantages, these methods provide limited information because they identify only viable organisms capable of growth under standard laboratory conditions. However, in order to carry out proper conservation and renovation, it is necessary to know the complete composition of microbial communities and their activity. This paper presents and characterizes modern techniques such as genetic fingerprinting and clone library construction for the assessment of microbial diversity based on molecular biology. Molecular methods represent a favourable alternative to culture-dependent methods and make it possible to assess the biodiversity of microorganisms inhabiting technical materials and cultural heritage objects.
    Źródło:
    Acta Biochimica Polonica; 2014, 61, 2; 217-225
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Genetic diversity among ethiopian coffee (Coffea Arabica L.) collections available in indian gene bank using sequence related amplified polymorphism markers
    Autorzy:
    Mishra, Manoj Kumar
    Nishani, Sandhyarani
    Gowda, Madhura
    Padmajyothi, Dandamudi
    Suresh, Narayana
    Sreenath, Hosahalli
    Raghuramulu, Y.
    Powiązania:
    https://bibliotekanauki.pl/articles/2199612.pdf
    Data publikacji:
    2014-12-18
    Wydawca:
    Instytut Hodowli i Aklimatyzacji Roślin
    Tematy:
    Coffea arabica L.
    Ethiopian germplasm
    Fingerprinting
    Genetic diversity
    SRAP marker
    Opis:
    The South-Western highlands of Ethiopia are considered to be the centre of origin and diversity of the arabica coffee, Coffea arabica. More than 80 accessions of arabica coffee collected from Ethiopia are avail-able in Indian gene bank. However, the genetic diversity of these accessions is not studied in detail. In the present study, genetic diversity analysis of 48 accessions collected from eight provinces of Ethiopia was car-ried out using Sequence-related amplified Polymorphism (SRAP) marker. Among the thirty two SRAP primer combinations tested, 14 primer pairs were polymorphic and generated 203 distinct fragments. The number of fragments ranged from 7 to 21 with a mean of 14.5 fragments per primer combination. Of the total 203 ampli-fied fragments, 182 (89.65%) were polymorphic and the percent of polymorphism ranged from 53.84% to a maximum of 100% using different primers. The average resolving power (Rp) and average polymorphism information content (PIC) of the 14 SRAP primer combinations was 14.31 and 0.648 respectively. A total of 13 rare alleles were obtained from SRAP assays, of which six rare alleles were obtained from the accessions collected from Shoa province.The UPGMA clustering algorithm from SRAP analysis grouped the 48 coffee accessions into two major clusters. The accessions collected from particular province clustered together which could be attributed to the substantial gene flow between adjacent population and the influence of geographical origin on genetic diver-sity. The study demonstrated the existence of substantial genetic variation in Ethiopian germplasm which could be utilized in coffee germplasm conservation and improvement program.
    Źródło:
    Plant Breeding and Seed Science; 2014, 70; 29-40
    1429-3862
    2083-599X
    Pojawia się w:
    Plant Breeding and Seed Science
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    ISSR analysis of chosen Gleditsia accessions obtained from Polish collections
    Autorzy:
    Smolik, M
    Kubus, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/41549.pdf
    Data publikacji:
    2009
    Wydawca:
    Polska Akademia Nauk. Instytut Dendrologii PAN
    Tematy:
    ISSR technique
    Gleditsia
    Polish collection
    genetic variability
    fingerprinting
    dendrology
    Polska
    Opis:
    Species of Gleditsia show considerable morphological variability that makes them difficult to distinguish using either vegetative or floral characters. Honeylocusts, especially the thornless cultivars, are popular ornamental, shade, street, attractive landscape trees. In this study the ISSR technique was used to evaluate the range of genetic variability between seven genotypes of Gleditsia cultivated in Polish dendrological collections [Gleditsia caspica Desf., Gleditsia japonica Miq., Gleditsia japonica Miq. var. korainensis (= G. korainensis Nakai), Gleditsia triacanthos L., Gleditsia triacanthos L. (bulk), Gleditsia triacanthos f. inermis (L.) Zabel (bulk). Forty ISSR primers were tested and 18 were selected for their ability to produce clear and reproducible patterns of multiple bands.A total of 177 loci of 260-2600 bp were amplified, of which 89 (50%) were polymorphic, 14 (8%) monomorphic and 74 (42%) were accession-specific. Accession-specific ISSR loci were obtained for all of the seven accessions tested. A dendrogram generated using the UPGMA, based on a similarity measure of total character difference, showed that the Gleditsia accessions were clustered into two main groups (‘a’ and ‘b’). The first grup –‘a’– included: Gleditsia triacanthos L., Gleditsia triacanthos L. (bulk) and Gleditsia triacanthos f. inermis (L.) Zabel (similarity 0.61–0.75), the second –‘b’– included 2 species: Gleditsia japonica and Gleditsia japonica var. korainensis (similarity 0.43). Analysis of the phylogenetic similarity dendrogram has shown wide range of diversity between studied accessions. The clustering pattern obtained in our experiment was in agreement with the data based on morphological, allozyme and ITS analysis.
    Źródło:
    Dendrobiology; 2009, 62; 63-70
    1641-1307
    Pojawia się w:
    Dendrobiology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Investigation of asparagine deamidation in a SOD1-based biosynthetic human insulin precursor by MALDI-TOF mass spectrometry
    Autorzy:
    Bierczyńska-Krzysik, Anna
    Łopaciuk, Małgorzata
    Pawlak-Morka, Renata
    Stadnik, Dorota
    Powiązania:
    https://bibliotekanauki.pl/articles/1039302.pdf
    Data publikacji:
    2014
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    asparagine deamidation
    insulin precursor
    superoxide dismutase
    peptide mass fingerprinting
    MALDI-TOF MS
    Opis:
    A biosynthetic human insulin precursor displayed enhanced susceptibility to deamidation at one particular site. The present study was undertaken to monitor progress of precursor deamidation at successive manufacturing stages. MALDI-TOF/TOF MS in combination with controlled endoproteinase Glu-C and endoproteinase Asp-N proteolysis was used for rapid and unambiguous determination of deamidated residue within the investigated structure. Close inspection of isotopic distribution patterns of peptides resulting from enzymatic digestion enabled determination of distinct precursor forms occurring during the production process. Asn, Asp, isoAsp and succinimide derivatives of the amino acid at position 26 were unambiguously identified. These modifications are related to the leader peptide of a precursor encompassing amino acid sequence corresponding to that of superoxide dismutase [Cu-Zn] (SOD1 1, EC=1.15.1.1). Monitoring of precursor deamidation process at successive manufacturing stages revealed that the protein folding stage was sufficient for a prominent replacement of asparagine by aspartic and isoaspartic acid and the deamidated human insulin precursor constituted the main manufactured product. Conversion proceeded through a succinimide intermediate. Significant deamidation is associated with the presence of SNG motif and confirms results achieved previously on model peptides. Our findings highlight an essential role of the specific amino acid sequence on accelerated rate of protein deamidation. To our knowledge, this is the first time that such a dramatic change in the relative abundance of Asp and isoAsp resulting from protein deamidation process is reported.
    Źródło:
    Acta Biochimica Polonica; 2014, 61, 2; 349-357
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Identifying microbes from environmental water samples
    Autorzy:
    Mungs, Wenfa
    Powiązania:
    https://bibliotekanauki.pl/articles/1153739.pdf
    Data publikacji:
    2015
    Wydawca:
    Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
    Tematy:
    biomarker
    educational research
    inquiry-based
    mass spectrum fingerprinting
    microbial ecology
    microbial typing
    phylogeny
    ribosomal proteins
    scientific method
    taxonomy
    Opis:
    What is the microbe that we are dealing with? Whether it is cholera or anthrax, we want to know the disease-causing microorganism as quickly as possible since prompt identification of the causative organism would help control disease spread - and potentially save lives through provision of appropriate care and medication. Yet, despite the advent of rapid microbial identification tools – particularly those based on mass spectrometry – most undergraduate curricula continue to focus on culture- and nucleic acid-based identification techniques since they are widely used for detecting and identifying microbes in clinical and environmental samples. Mass spectrometry-based methods, however, have increasingly complemented traditional approaches in clinical and research laboratories - but are rarely featured in undergraduate curricula. Motivated by the desire to address the curriculum gap, the author of this study developed an inquiry-based laboratory exercise for introducing students to the operating principles and methodology of mass spectrometry-based microbial identification. By requiring students to identify microbes in environmental water samples – a real-life problem with unknown answers – the exercise piqued the students’ interest in learning, while helping to stir their curiosity through an interesting field activity in which they could put on a scientist’s hat in solving a mystery. This synopsis article summarizes a piece of published educational research and expands on the discussion of concepts underlying matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS)-based microbial identification. Herein, the article discusses the relative advantages and disadvantages of the pattern recognition and proteome database search approaches for analyzing mass spectra data. Additionally, the effect of general and tailored sample preparation protocols on identification accuracy is also elaborated. Finally, the pedagogical utility of field- and inquiry-based educational tools is also discussed in greater detail from a post-publication perspective. A full-length synopsis of the work and a structured abstract can be found in the accompanying PDF file, the original article being entitled: “Teaching Microbial Identification with Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) and Bioinformatics Tools”.
    Źródło:
    World News of Natural Sciences; 2015, 2; 7-19
    2543-5426
    Pojawia się w:
    World News of Natural Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Genetic diversity of inbred rye lines evaluated by RAPD analysis
    Autorzy:
    Myskow, B
    Masojc, P.
    Banek-Tabor, A.
    Szolkowski, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/2041926.pdf
    Data publikacji:
    2001
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    random amplified polymorphic DNA
    rye line
    rye
    Secale cereale
    fingerprinting
    hybrid rye
    hybrid cultivar
    genetic information
    genetic similarity
    plant breeding
    Źródło:
    Journal of Applied Genetics; 2001, 42, 1; 1-14
    1234-1983
    Pojawia się w:
    Journal of Applied Genetics
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Fast separation and detection of main components in complex raw biological materials using temperature-controlled planar micro-chromatography (micro-TLC)
    Szybkie rozdzielanie i detekcja głównych składników złożonych materiałów biologicznych za pomocą mikrochromatografii planarnej (micro-TLC) prowadzonej w warunkach kontrolowanej temperatury
    Autorzy:
    Zarzycki, P. K.
    Ślączka, M. M.
    Zarzycka, M. B.
    Włodarczyk, E.
    Baran, M. J.
    Heese, T.
    Głód, B. K.
    Powiązania:
    https://bibliotekanauki.pl/articles/158415.pdf
    Data publikacji:
    2010
    Wydawca:
    Stowarzyszenie Inżynierów i Techników Mechaników Polskich
    Tematy:
    chromatografia planarna
    micro-TLC
    liofilizacja
    żółć ryb
    troć wędrowna (Salmo trutta m. trutta)
    kwasy żółciowe
    spirulina
    preparaty farmaceutycze
    detekcja fluorymetryczna
    kwas fosforomolibdenowy
    temperatura
    frakcjonowanie
    fingerprinting
    planar chromatography
    lyophilization
    fish bile
    sea trout (Salmo trutta m. trutta)
    bile acids
    pharmaceutical formulations
    fluorimetric detection
    phosphomolybdic acid
    temperature
    fractionation
    Opis:
    This paper is a continuation of our previous research focusing on development of micro-TLC methodology. The main goal of present paper is to demonstrate the separation and detection capability of micro-TLC technique involving simple analytical protocols without multi-steps sample pre-purification. In present studies components of interest were isolated from biological samples including fish bile and spirulina cells. Described methodology can be applied for fast fractionation or screening of target substances as well as chemo-taxonomic studies and fingerprinting of complex mixtures, which are present in raw biological or environmental samples.
    Chromatografia cienkowarstwowa jest powszechnie wykorzystywaną metodą analityczną stosowaną w rozdzielaniu substancji obecnych w złożonych próbkach biologicznych, środowiskowych oraz preparatach farmaceutycznych. Związane jest to głównie z prostotą sprzętu, możliwością jednoczesnej analizy wielu próbek w trakcie jednego procesu analitycznego. Dodatkowo, istotna jest możliwość bezpośredniego analizowania złożonych próbek bez ich wstępnego oczyszczenia. Obecna publikacja jest kontynuacją badań dotyczących zastosowania mikrochromatografii planarnej prowadzonej w warunkach kontrolowanej temperatury (rys. 1). W szczególności, w pracy wykazano potencjał analityczny mikrochromatografii planarnej w rozdzielaniu złożonych próbek, bez potrzeby ich uprzedniego wieloetapowego oczyszczania.Jako materiały badane wykorzystano spirulinę oraz żółć rybią (rys. 2 - 4). Wykazano, iż detekcja analitów może być znacząco poprawiona poprzez umieszczenie uprzednio rozwiniętych płytek chromatograficznych w parach jodu lub poprzez wybarwienie plamek odczynnikiem wywołującym (kwasem fosforomolibdenowym). Niskocząsteczkowe substancje z próbek środowiskowych (wody z jezior, ścieki surowe oraz oczyszczone) były zatężane przy pomocy ekstrakcji do fazy stałej. Badania wykazały obecność szeregu pasm na mikrochromatogramach cienkowarstwowych, które są specyficzne dla poszczególnych ekosystemów wodnych Pomorza Środkowego. Opisana metodologia może znaleźć zastosowanie w szybkim frakcjonowaniu oraz oznaczaniu ilościowym substancji niskocząsteczkowych pochodzących ze złożonych materiałów biologicznych, jak również w badaniach przesiewowych dużej ilości próbek środowiskowych (rys. 5 - 8).
    Źródło:
    Pomiary Automatyka Kontrola; 2010, R. 56, nr 4, 4; 360-364
    0032-4140
    Pojawia się w:
    Pomiary Automatyka Kontrola
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-9 z 9

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