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Wyświetlanie 1-15 z 28
Tytuł:
The technique of flow cytometry in diagnostic research
Autorzy:
Macheta, A.
Radko, K
Szymczyk, S.
Podhorecka, M
Powiązania:
https://bibliotekanauki.pl/articles/102250.pdf
Data publikacji:
2013
Wydawca:
Stowarzyszenie Inżynierów i Techników Mechaników Polskich
Tematy:
flow cytometry
immunophenotype
hematooncology
Opis:
Flow cytometry is a technology that simultaneously counts and then examines multiple physical and/or chemical characteristics of single particles, usually cells, as they flow in a fluid stream through a beam of light. The properties measured include a particle’s relative size, relative granularity or internal complexity, and relative fluorescence intensity. These characteristics are determined using an optical-to-electronic coupling system that records how the cell scatters incident laser light and emits fluorescence. One of the most significant applications is immunophenotyping of cells - the most important tool in diagnosis and monitoring haematological disorders, such as acute and chronic leukemia, lymphoma, monoclonal gammopathy, myelodisplastic and myeloproliferative diseases.
Źródło:
Advances in Science and Technology. Research Journal; 2013, 7, 19; 55-59
2299-8624
Pojawia się w:
Advances in Science and Technology. Research Journal
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Cytostatic and cytotoxic effects of (E)-2'-deoxy-2'-(fluoromethylene)-cytidine on a solid tumor and a leukemia cell line.
Autorzy:
Grieb, Paweł
Koronkiewicz, Mirosława
Skierski, Janusz
Powiązania:
https://bibliotekanauki.pl/articles/1044427.pdf
Data publikacji:
2000
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
cytotoxicity
flow cytometry
fluoromethylenedeoxycytidine
apoptosis
Opis:
(E)-2'-deoxy-2'-(fluoromethylene)-cytidine (FMdC), a deoxycytidine analog displaying a very high toxicity toward a variety of solid tumor cell lines and xenografts, is activated intracellularly by deoxycytidine kinase (dCK). We have compared cytotoxicity of FMdC towards a human promyeolocytic leukemia line HL-60 and a human colorectal carcinoma line COLO-205. Despite dCK activity being by far the highest in cells of lymphoid origin, the effects of FMdC were detectable at the lowest drug concentration only in a solid tumor cell line, and at higher concentrations they were qualitatively similar in the two tumor lines (increased cell protein content, cell cycle block and apoptosis). Apparently, low dCK activity in solid tumor cells sufficiently activates FMdC to yield cytotoxic effects, while high dCK activity in leukemia cells does not increase its cytotoxicity.
Źródło:
Acta Biochimica Polonica; 2000, 47, 1; 165-171
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Studying Microbiology of Rain Water For of Their Use in Economy
Autorzy:
Zdeb, M.
Zamorska, J.
Papciak, D.
Powiązania:
https://bibliotekanauki.pl/articles/124199.pdf
Data publikacji:
2016
Wydawca:
Polskie Towarzystwo Inżynierii Ekologicznej
Tematy:
rainwater
microbial contamination
flow cytometry
plate method
Opis:
Growing areas of sealed surfaces, rising water needs due to industry development, increasing populations, and climatic changes affect precipitation patterns and form a vision of the future in which meteoric water storage may become almost an obligatory activity. The aim of this paper was to identify the amounts and, to some degree, the quality of microorganisms present in rainwater collected from different types of rooftops of utility buildings in the spring-summer season. Apart from the classic culture plate method complemented by flow cytometry. The results of performed analyses explicitly show that rainwater collected from rooftops and directly from air prove to be microbially contaminated to a substantial degree, which includes pathogenic coliforms and faecal streptococci. Waters collected after dry periods also contained bacteria like Clostridium perfingens. The findings rule out the possibility of using rainwater collected from roof surfaces of utility buildings before its treatment.
Źródło:
Journal of Ecological Engineering; 2016, 17, 3; 203-208
2299-8993
Pojawia się w:
Journal of Ecological Engineering
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Optimization of detection of circulating tumor cells by flow cytometry and qRT-PCR
Autorzy:
Pogoda, Katarzyna
Rybojad, Paweł
Álvarez, Inés Ramírez
Mohanathas, Thusyanthan
Hosseini, Sarah
Tabarkiewicz, Jacek
Powiązania:
https://bibliotekanauki.pl/articles/454775.pdf
Data publikacji:
2019
Wydawca:
Uniwersytet Rzeszowski. Wydawnictwo Uniwersytetu Rzeszowskiego
Tematy:
circulating tumor cells
flow cytometry
PCR
Opis:
Introduction. Treatment and diagnostic process in solid tumors like lung cancer are still based on invasive methods such as bronchoscopy, solid biopsy et cetera. One of the less invasive methods is a proposed “liquid biopsy” which is based on capturing of tumor cells circulating in the blood. Aim. The aim of the study was to standardize conditions and to assess the sensitivity of the identification of circulating tumor cells (CTCs) with the use of flow cytometry and qRT-PCR. Material and methods. In the first model of CTCs, cells from the A549 lung cancer cell line were suspended in 1 ml of healthy donors’ blood in 5 spikes increasingly: 0, 10, 50, 100 and 200 and the cells were detected in flow cytometer. In the second model, cells from the A549 and H1975 lung cancer cell lines were used. Spikes were prepared as in the first model, but cells were suspended in 400 µl of healthy donors’ blood and were detected with the use of qRT-PCR. Results. An increasing number of detected cytokeratin positive events from the 1st spike “0” to the last one - “200” was observed by flow cytometry. Median value in the negative control was 0 false positive cells. In tubes from “10” to “200” the median was 5, 43.5, 58 and 78, respectively. Mean sensitivity of flow cytometry was 63.79%. In qRT-PCR, correlation between increasing number of sorted cells in several spikes and the level of mRNA expression for KRT19 gene was not observed. Conclusion. Commonly available methods like flow cytometry and qRT-PCR seem to be attractive solutions for CTCs detection, but they need pre-enrichment procedures and standardization
Źródło:
European Journal of Clinical and Experimental Medicine; 2019, 1; 5-11
2544-2406
2544-1361
Pojawia się w:
European Journal of Clinical and Experimental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Anticancer activity of new molecular hybrids combining 1,4-naphthalenedione motif with phosphonic acid moiety in hepatocellular carcinoma HepG2 cells
Autorzy:
Długosz, Angelika
Gach, Katarzyna
Szymański, Jacek
Modranka, Jakub
Janecki, Tomasz
Janecka, Anna
Powiązania:
https://bibliotekanauki.pl/articles/1038683.pdf
Data publikacji:
2017
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
MTT test
apoptosis
real-time PCR
flow cytometry
Opis:
Structural motifs found in naturally occurring compounds are frequently used by researchers to develop novel synthetic drug candidates. Some of these new agents are hybrid molecules which are designed through a concept of combining more than one functional element. In this report, anticancer activity of new synthetic molecular hybrids, substituted 3-diethoxyphosphorylnaphtho[2,3-b]furan-4,9-diones and 3-diethoxyphosphorylbenzo[f]indole-4,9-diones, which integrate natural 1,4-naphtalenedione scaffold, present in several anticancer agents, with pharmacophoric phosphonate moiety, were tested against hepatocellular cell line HepG2. Cytotoxicity was examined using MTT assay. Two most potent compounds, furandione 8a and benzoindoldione 12a, which reduced the number of viable HepG2 cells with the IC50 values of 4.13 µM and 5.9 µM, respectively, were selected for further research. These compounds decreased the mRNA expression levels of several genes: Bcl-2, angiogenic vascular endothelial growth factor (VEGF), c-Fos, caspase-8 and increased the expression of Bax, caspase-3 and -9, c-Jun, p21, p53, as determined by quantitative real-time PCR. The ability of these compounds to induce apoptosis and DNA damage was studied by flow cytometry. The obtained data showed that the new compounds inhibited cell viability by increasing apoptosis and decreasing angiogenesis. Compound 8a was a much stronger apoptosis inducer as compared with 12a and strongly activated the intrinsic pathway of apoptosis, associated with the loss of mitochondrial membrane potential and changes in Bax/Bcl-2 ratio. These findings show that the synthetic hybrids combining 1,4-naphthalenedione system and phosphonic acid moiety display potential to be further explored in the development of new anticancer agents.
Źródło:
Acta Biochimica Polonica; 2017, 64, 1; 41-48
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Influence of bovine follicular fluid on thawed bovine spermatozoa – assessment by CASA system and flow cytometry
Autorzy:
Mrowiec, J.
Twardoń, J.
Bartoszewicz, A.
Niżański, W.
Ochota, M.
Powiązania:
https://bibliotekanauki.pl/articles/2087540.pdf
Data publikacji:
2019
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
bovine follicular fluid
spermatozoa
CASA
flow cytometry
Źródło:
Polish Journal of Veterinary Sciences; 2019, 2; 305-312
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Evaluation of circulating microparticles in healthy medical workers occupationally exposed to ionizing radiation: A preliminary study
Autorzy:
Al-Massarani, Ghassan
Najjar, Fadi
Aljapawe, Abdulmunim
Ikhtiar, Adnan
Powiązania:
https://bibliotekanauki.pl/articles/2162036.pdf
Data publikacji:
2018-12-20
Wydawca:
Instytut Medycyny Pracy im. prof. dra Jerzego Nofera w Łodzi
Tematy:
biomarkers
flow cytometry
occupational exposure
microparticles
ionizing radiation
cellular damage
Opis:
Objectives Ionizing radiation was known to cause disruption of cytoskeleton. However, the disorganization of the cytoskeleton leads to form microparticles (MP) that carry membrane and cytoplasmic constituents from their parent cells they are released from. Therefore, authors investigated the effect of the occupational exposure to low doses of ionizing radiation on MP levels. Material and Methods The current study was conducted on 38 healthy medical workers occupationally exposed to low doses of ionizing radiation and 29 controls matched by gender, age, and smoking habits. The MP levels measured by flow cytometry were classified as positive or negative phosphatidylserine (PS⁺ or PS⁻), and phenotyped according to their cellular origin. Results Total MP (PS–/PS+) levels, regardless of phenotype, were significantly higher in workers occupationally exposed to ionizing radiation than in healthy individuals (p = 0.0004). Negative phosphatidylserine microparticles were predominant in medical exposed workers and, to a lesser extent, in controls (68% and 57%, respectively). With regard to phenotypic characterization of cellular origin, MP derived from platelets (CD41a+), endothelial (CD146+), leucocytes (CD45+) and erythrocytes (CD235a+) MP were significantly enhanced in exposed workers compared with controls (p < 0.0001). However, no significant difference was found in the proportion of the other blood elements in the peripheral circulation between the 2 groups. Serum levels of C-reactive protein were normal for all individuals. In addition, no association was observed between MP levels and the studied confounding factors. Conclusions The results suggest that elevated circulating MP levels represent an indicator of cellular damage caused by medical exposure to low doses of ionizing radiation. By consequence, the quantification of MP seems to be a useful biomarker for assessing the negative effects of occupational exposure to ionizing radiation. Int J Occup Med Environ Health 2018;31(6):783–793
Źródło:
International Journal of Occupational Medicine and Environmental Health; 2018, 31, 6; 783-793
1232-1087
1896-494X
Pojawia się w:
International Journal of Occupational Medicine and Environmental Health
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Biological Stability of Water after the Biofiltration Process
Autorzy:
Zamorska, J.
Powiązania:
https://bibliotekanauki.pl/articles/124588.pdf
Data publikacji:
2018
Wydawca:
Polskie Towarzystwo Inżynierii Ekologicznej
Tematy:
biofiltration
microbiology of water
biological stability of water
flow cytometry
Opis:
One of the most frequently implemented processes in the water treatment technology is filtration through a biosorption bed. The techniques based on biochemical processes involving bacteria result in obtaining high quality of water. There are a number of different materials used as the filler material for biological filters. Carbon deposits are the most popular, due to their high effectiveness. The problem with the use of this process is the leaching of microorganisms from the biofilm and the biological stability of water thus obtained. There is a need to develop quick methods to assess the microbiological quality of this water. Modern techniques for determining the amount of microorganisms, such as flow cytometry and luminometry may be the right tools. The water collected for testing came from the Water Treatment Station located in the Podkarpackie voivodeship. The microbiological tests carried out in the analyzed water samples collected after the filtration process on granular activated carbon. Both traditional culture method and modern techniques used to determine the number of microorganisms (flow cytometry, luminometric ATP assay) demonstrated an increase in the number of microorganisms in the examined waters (in the water after the filtration process and in the water introduced into the water supply network) after the incubation process for 3 and 7 days at 15 and 22°C.
Źródło:
Journal of Ecological Engineering; 2018, 19, 5; 234-239
2299-8993
Pojawia się w:
Journal of Ecological Engineering
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Estimation of the share of components of different ploidy in anisoploid sugar-beet [Beta vulgaris L.] populations by flow cytometry
Autorzy:
Sliwinska, E
Jansen, R
Powiązania:
https://bibliotekanauki.pl/articles/2046829.pdf
Data publikacji:
1997
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Beta vulgaris
ploidy determination
breeding
flow cytometry
seed production
sugar-beet
Opis:
Flow cytometry is commonly used in sugar-beet breeding and seed production for ploidy estimation. By means of this method individual plants as well as plant populations can be analysed. An interpretation of histograms obtained from anisoploid populations, however, causes some problems to the breeder. Bulks of diploid, triploid and tetraploid sugar-beet plants in different proportion and ploidy combinations (2x + 3x, 2x + 4x, and 3x + 4x) were tested in order to determine the relation between the actual share of the components of different ploidy levels in tested populations and that calculated on the basis of flow cytometry. In this way agraph for estimation of the share of different ploidy components was obtained. Repeatability of the results proves the usefulness of flow cytometric analysis of anisoploid plant populations in breeding and seed production of polyploid crops.
Źródło:
Journal of Applied Genetics; 1997, 38, 2; 151-160
1234-1983
Pojawia się w:
Journal of Applied Genetics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Cytotoxic effects of cladribine and tezacitabine toward HL-60.
Autorzy:
Stachnik, Krzysztof
Grieb, Paweł
Skierski, Janusz
Powiązania:
https://bibliotekanauki.pl/articles/1041452.pdf
Data publikacji:
2005
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
tezacitabine
cladribine
flow cytometry
nucleoside analogs
leukemia
HL-60 cells
Opis:
The aim of the study was to determine the relation between the cytotoxic and cytostatic effects of tezacitabine and cladribine on a HL-60 cell line and the time of exposure of cells to these drugs. Cell viability and induction of apoptosis were assessed using flow cytometry methods. Apoptosis was confirmed by direct microscopic observation. Growth inhibition was examined by cell counting. After 24 h incubation tezacitabine was equally or less toxic compared to cladribine. However, toxicity of tezacitabine strongly rose after 48 h incubation leading to massive cell death at doses much lower than those of cladribine. Assessment of the effect of increased exposure time on the clinical efficacy of tezacitabine is indicated.
Źródło:
Acta Biochimica Polonica; 2005, 52, 2; 561-565
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The flavonoids, quercetin and isorhamnetin 3-O-acylglucosides diminish neutrophil oxidative metabolism and lipid peroxidation.
Autorzy:
Zielińska, Małgorzata
Kostrzewa, Artur
Ignatowicz, Ewa
Budzianowski, Jaromir
Powiązania:
https://bibliotekanauki.pl/articles/1044184.pdf
Data publikacji:
2001
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
flow cytometry
respiratory burst
polymorphonuclear neutrophils
reactive oxygen species
lipid peroxidation
flavonoids
Opis:
Two natural flavonoids, quercetin and isorhamnetin 3-O-acylglucosides, were examined for their inhibitory influence on the in vitro production and release of reactive oxygen species in polymorphonuclear neutrophils (PMNs). The generation of superoxide radical, hydrogen peroxide and hypochlorous acid were measured by, respectively, cytochrome c reduction, dichlorofluorescin oxidation and taurine chlorination. Membrane lipid oxidation was studied by the thiobarbituric acid method in mouse spleen microsomes. The addition of flavonoids at the concentration range 1-100 μM inhibited PMNs oxidative metabolism and lipid peroxidation in a dose-dependent manner. The results suggest that these flavonoids suppress the oxidative burst of PMNs and protect membranes against lipid peroxidation.
Źródło:
Acta Biochimica Polonica; 2001, 48, 1; 183-189
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Different MACS sorting strategies for the enrichment of Lin– (CD34+ CD45–) hematopoietic progenitor cells: preliminary study
Autorzy:
Vašíček, Jaromír
Baláži, Andrej
Parkányi, Vladimír
Bauer, Miroslav
Powiązania:
https://bibliotekanauki.pl/articles/2183569.pdf
Data publikacji:
2018-12-31
Wydawca:
Uniwersytet Pedagogiczny im. Komisji Edukacji Narodowej w Krakowie
Tematy:
CD34
CD45
flow cytometry
hematopoietic stem cells
MACS
qPCR
rabbit
Opis:
Magnetic-activated cell sorting (MACS) has become a standard method for the isolation of hematopoietic stem/progenitor cells (HSC/HPC) in human or mouse model using CD34 antibodies. However, at the present there is no useable CD34 antibody that could be successfully used for the selection of rabbit HSC/HPC. Therefore, the aim of this preliminary study was to remove all mature cells (CD45+) from the heterogeneous mixture of rabbit peripheral blood and bone marrow mononuclear cells (PBMCs and BMMCs) in order to enrich these cell populations for the CD34+ cells. Briefly, cells were incubated with a CD45 antibody and proper magnetic microbeads. Three different MACS sorting strategies were used in the experiment that differed mainly in the sample loading rate and the number of used magnetic columns. Control (unsorted) and sorted cells were assessed for the sorting efficiency (% of double positive cells for CD45 and Labelling Check Reagent - LCR) by flow cytometry and for the relative expression of CD34 antigen by qPCR. According to flow cytometry, Depl025 mode showed the best sorting efficiency in terms of the lowest percentages of CD45+LCR+ cells for rabbit PBMCs as well as BMMCs. qPCR analysis confirmed this mode as the best in terms of the relative CD34 expression for rabbit PBMCs. However, higher relative expression of CD34 in BMMCs was obtained by other mode - Posselds. In conclusion, this study demonstrates a possible enrichment of rabbit (CD34+) HSC/HPC by the magnetic depletion of mature hematopoietic (CD45+) cells.
Źródło:
Annales Universitatis Paedagogicae Cracoviensis Studia Naturae; 2018, 3 (suppl.); 83-89
2543-8832
2545-0999
Pojawia się w:
Annales Universitatis Paedagogicae Cracoviensis Studia Naturae
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Validation of a flow cytometry-based assay for monitoring complement-dependent cytotoxicity of an anti-CD20 biosimilar candidate antibody
Autorzy:
Blanco-Santana, R.
Cedeno-Arias, M.
Garcia-Perez, Z.
Columbie-Gilbert, D.
Portillo-Vaquer, A.
Rengifo-Calzado, E.
Powiązania:
https://bibliotekanauki.pl/articles/80888.pdf
Data publikacji:
2014
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
flow cytometry
validation
complement-dependent cytotoxicity
monoclonal antibody
CD20 antigen
biological activity
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2014, 95, 1
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Induction of the multixenobiotic/multidrug resistance system in various cell lines in response to perfluorinated carboxylic acids
Autorzy:
Rusiecka, Izabela
Składanowski, Andrzej
Powiązania:
https://bibliotekanauki.pl/articles/1040751.pdf
Data publikacji:
2008
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
multixenobiotic resistance
multidrug resistance
perfluorinated carboxylic acids
flow cytometry
ABCB1
ABCC1
Opis:
The multixenobiotic resistance (closely related to multidrug resistance) system controls transport across the plasma membrane as a defense against toxic molecules. Multixenobiotic resistance system consists of an efflux pump, ABCB1 (also named P-glycoprotein, P-gp), and/or a molecule of the ABCC family (also named multiple resistance associated protein, MRP). ABCB1 is able to increase efflux of many low-molecular foreign molecules. Measuring system induction may be used as a biomarker of cell/organism exposure to foreign substances. Various established cell lines were tested for constitutive and induced multixenobiotic resistance proteins by Western blotting immunodetection. The pumping function was indirectly assayed with Rhodamine B by visualization of cell fluorescence in the presence of verapamil. Changes in ABC proteins were measured by flow cytometry after exposition to various perfluorinated carboxylic acids. MCF7 and HeLa cells were found to contain the highest constitutive level of both ABCB1 and ABCC1. HEK293 exhibited much less ABCB1 and no activity of pumping out Rhodamine B. The pumping activity was found to be related to the amount of the cell-type specific 170 kDa ABCB1 protein. An 8-day exposure to 10-4 M perfluorononanoic acid resulted in about 2-2.5-fold increase of ABCB1 level. That was confirmed also for short times by flow cytometry of cells exposed to perfluorinated acids and its natural congeners. Both ABCB1- and ABCC1-related fluorescence increased along with the carbon chain in acids from C6 up to C9 and decreased for C10. Measuring of multixenobiotic resistance changes in vitro induced by chemicals may be a convenient test for screening for their potential toxicity.
Źródło:
Acta Biochimica Polonica; 2008, 55, 2; 329-337
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Partial characterization of human choriocarcinoma cell line JAR cells in regard to oxidative stress.
Autorzy:
Hallmann, Anna
Klimek, Jerzy
Masaoka, Makoto
Kamiński, Marcin
Kędzior, Jakub
Majczak, Anna
Niemczyk, Edyta
Woźniak, Michał
Trzonkowski, Piotr
Wakabayashi, Takashi
Powiązania:
https://bibliotekanauki.pl/articles/1041517.pdf
Data publikacji:
2004
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
menadione
flow cytometry
JAR cells
placenta
H2O2
oxidative stress
Opis:
Characterization of free radical-induced cell injury processes of placenta cells is of vital importance for clinical medicine for the maintenance of intrauterine fetal life. The present study has analyzed cell injury processes in cells of the choriocarcinoma cell line JAR treated with menadione, an anticancer drug, and Hg2O2 in comparison to osteosarcoma 143B cells using electron microscopic and flow cytometric techniques. Flow cytometry on JAR cells exposed to 100 μM menadione and double-stained with Annexin V and propidium iodide (PI) detected apoptotic cells reaching the maximum after 4 h of incubation with a rapid decrease thereafter. Viable cells became decreased to 46% of the control after 2 h of incubation, reaching 5% after 4 h. Cells stainable with both Annexin V and PI began to increase distinctly after 2 h of incubation, reaching 55% after 4 h. Electron microscopy showed that cells stainable with both dyes specified above had condensed nuclei and swollen cytoplasm, suggesting that they were undergoing a switch of the cell death mode from apoptosis to necrosis. On the other hand, 90% of 143B cells remained intact after 4 h of menadione treatment although the intracellular levels of superoxide were always higher than those of JAR cells treated with the drug. In contrast, JAR cells were more resistant than 143B cells to H2O2-induced cytotoxicity. These results may suggest that cytotoxicity of menadione cannot be explained simply by oxygen free radicals generated from the drug. The resistance of JAR cells to oxygen free radical-induced cytotoxicity may be advantageous for intrauterine fetal life.
Źródło:
Acta Biochimica Polonica; 2004, 51, 4; 1023-1038
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
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