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    Wyświetlanie 1-4 z 4
    Tytuł:
    Immobilization of Aspergillus oryzae Β galactosidase on concanavalin A-layered calcium alginate-cellulose beads and its application in lactose hydrolysis in continuous spiral bed reactors
    Autorzy:
    Ansari, S. A
    Husain, Q.
    Powiązania:
    https://bibliotekanauki.pl/articles/779751.pdf
    Data publikacji:
    2011
    Wydawca:
    Zachodniopomorski Uniwersytet Technologiczny w Szczecinie. Wydawnictwo Uczelniane ZUT w Szczecinie
    Tematy:
    Β galaktozydazy
    unieruchomienie
    celulozowy alginian
    konkanawalina A
    reaktor spiralny
    hydroliza laktozy
    β-galactosidase
    immobilization
    cellulose-alginate
    concanavalin A
    spiral bed reactor
    lactose hydrolysis
    Opis:
    In this study, Aspergillus oryzae Β galactosidase was immobilized on concanavalin A layered calcium alginate-cellulose beads as a bioaffi nity support. Immobilized enzyme showed a remarkable broadening in temperature-activity profi les as compared to the native enzyme and exhibited 65% activity in the presence of 5% galactose. Michaelis constant (Km) was 2.57 mM and 5.38 mM for the free and the immobilized Β galactosidase, respectively. Crosslinked Β galactosidase showed greater catalytic activity in the presence of Mg2+ and was more stable during storage at 4°C for 6 weeks. Immobilized enzyme hydrolyzed 67% lactose in milk in 8 h and 85% lactose in whey in 9 h in the stirred batch process at 50oC. The continuous hydrolysis of lactose by crosslinked Β galactosidase in spiral bed reactor exhibited 93% and 88% hydrolysis of lactose at flow rate of 20 ml/h and 30 ml/h, after 1 month operation, respectively.
    Źródło:
    Polish Journal of Chemical Technology; 2011, 13, 4; 15-20
    1509-8117
    1899-4741
    Pojawia się w:
    Polish Journal of Chemical Technology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Hydrogels based on ionically and covalently crosslinked alginates
    Autorzy:
    Walczak, J.
    Marchewka, J.
    Laska, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/284594.pdf
    Data publikacji:
    2014
    Wydawca:
    Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
    Tematy:
    hydrogels
    alginate beads
    crosslinking
    cellulose
    Opis:
    In the presented research sodium alginate modified by ionic and covalent crosslinking in the form of hydrogel beads was examined. In the first step, sodium alginate was ionically crosslinked by treatment with calcium chloride. Different forms of crosslinked alginate were obtained depending on the calcium chloride concentration. The forms varied from shapeless gel to dense agglomerated spheres. The optimal structure were spherical beads separated from each other. Ionically crosslinked alginates were then subjected to covalent crosslinking with epichlorohydrin. For additional reinforcement the crosslinked beads of alginate were modified with cellulose nanofibers. The products were compared in terms of water absorption in the dry samples, their morphology was observed by scanning electron microscopy. Calcium ions distribution in the composites was evaluated by energy dispersive spectroscopy analysis. In vitro biodegradation of the crosslinked alginates was also investigated.
    Źródło:
    Engineering of Biomaterials; 2014, 17, no. 128-129; 109-110
    1429-7248
    Pojawia się w:
    Engineering of Biomaterials
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Functional three-component polymeric biocomposites for the treatment of bedsores
    Autorzy:
    Wiśniewska-Wrona, Maria
    El Fray, Mirosława
    Powiązania:
    https://bibliotekanauki.pl/articles/1034423.pdf
    Data publikacji:
    2018
    Wydawca:
    Sieć Badawcza Łukasiewicz - Polskie Towarzystwo Chitynowe
    Tematy:
    Chitosan
    FTIR
    NMR
    alginate
    carboxymethyl cellulose
    dressing DSC
    Opis:
    Presented here are the results of investigations into the preparation of three-component dressing materials from various biopolymers in the form of a single-layer film which is suitable as a carrier for pain-relieving (lidocaine) and bacteriostatic (sulphanilamid) therapeutic agents. Physical-chemical, biological and usable properties of the prepared materials were tested and assessed. The amount of added active substance was adopted based on the dose recommended by the Polish Pharmacopeia for external medicinal preparations. Antibacterial activity against gram (-) Escherichia coli and gram (+) Staphylococcus aureus was assessed in some of the biocomposites by quantitative methods. The cytotoxic action in direct contact with the mouse fibroblast NCTC clone 929 was also estimated. Thermal analysis (DSC), infrared spectrophotometry (FTIR) and nuclear magnetic resonance spectroscopy were employed to investigate the impact of the variable contents of chitosan, alginate, carboxymethyl cellulose (CMC), and the active substance upon the chemical- and phase-structure of the prepared three-component polymeric biocomposites. It was found that the quantitative composition of the biocomposites and the additive of active substances lidocaine and sulphanilamide exert a vital impact upon their physical-mechanical and usable properties (imbibition, absorption). Investigations into the release of the medicinal substance from the investigated biocomposites to an acceptor fluid led to the conclusion that the kinetics of the process may be described by a complex first order rate equation with two exponential functions.
    Źródło:
    Progress on Chemistry and Application of Chitin and its Derivatives; 2018, 23; 185-206
    1896-5644
    Pojawia się w:
    Progress on Chemistry and Application of Chitin and its Derivatives
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Are agricultural and natural sources of bio-products important for modern regenerative medicine? A review
    Autorzy:
    Nowacki, Maciej
    Nowacka, Katarzyna
    Kloskowski, Tomasz
    Pokrywczyńska, Marta
    Tyloch, Dominik
    Rasmus, Marta
    Warda, Karolina
    Drewa, Tomasz
    Powiązania:
    https://bibliotekanauki.pl/articles/989866.pdf
    Data publikacji:
    2017
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    cellulose
    plant extract
    alginate
    chitosan
    porcine scaffold
    small intestine submusocsa (sis)
    bladder acellular matrix (bam)
    Opis:
    Introduction and objectives. As tissue engineering and regenerative medicine have continued to evolve within the field of biomedicine, the fundamental importance of bio-products has become increasingly apparent. This true not only in cases where they are derived directly from the natural environment, but also when animals and plants are specially bred and cultivated for their production. Objective. The study aims to present and assess the global influence and importance of selected bio-products in current regenerative medicine via a broad review of the existing literature. In particular, attention is paid to the matrices, substances and grafts created from plants and animals which could potentially be used in experimental and clinical regeneration, or in reconstructive procedures. Summary. Evolving trends in agriculture are likely to play a key role in the future development of a number of systemic and local medical procedures within tissue engineering and regenerative medicine. This is in addition to the use of bio-products derived from the natural environment which are found to deliver positive results in the treatment of prospective patients.
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2017, 24, 2
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
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