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Wyświetlanie 1-12 z 12
Tytuł:
Densitometric quantification of DNA topoisomers in ethidium bromide-stained agarose gels and chemiluminescence-detected X ray films.
Autorzy:
Topcu, Zeki
Powiązania:
https://bibliotekanauki.pl/articles/1044334.pdf
Data publikacji:
2000
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
densitometry
ethidium bromide
chemiluminescence
DNA topoisomers
Opis:
This study investigates the contribution of deformational strain imposed by topological interconversions of DNA in ethidium bromide-binding on agarose gels. Closed-circular plasmid DNAs were nicked using UV exposure and the DNA bands were quantified by densitometry. The results show that the closed circular DNA binds the same amount of the dye as its nicked counterpart. The relationship between the band intensity on X-ray films of chemiluminescence-detected Southern blots and DNA concentration was shown to be linear.
Źródło:
Acta Biochimica Polonica; 2000, 47, 3; 835-839
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Preparation and characterization of ultra-fine silver bromide suspension
Autorzy:
Król-Gracz, A.
Michalak, E.
Nowak, P.
Dyonizy, A.
Powiązania:
https://bibliotekanauki.pl/articles/115949.pdf
Data publikacji:
2011
Wydawca:
Fundacja na Rzecz Młodych Naukowców
Tematy:
nanoparticles
silver bromide
synthesis method
gelatine
Opis:
This paper present the results of studies on ultra fine-crystalline silver bromide suspensions, which were stabilized by gelatin and excess of bromide ions. Relation between dosing rate of reactants to size of obtained silver bromide crystals was investigated. Granulometric properties obtained nanocrystals were studied by turbidity method and by technique of Dynamic Light Scattering (DLS). Additionally, a photomicrograph of suspension using Scanning Electron Microscopy (SEM) was performed.
Źródło:
Challenges of Modern Technology; 2011, 2, 2; 38-42
2082-2863
2353-4419
Pojawia się w:
Challenges of Modern Technology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Assessment of a new scintillation crystal (LaBr3) in PET scanners using Monte Carlo method
Autorzy:
Karimian, A. E.
Thompson, Ch. J.
Powiązania:
https://bibliotekanauki.pl/articles/146815.pdf
Data publikacji:
2008
Wydawca:
Instytut Chemii i Techniki Jądrowej
Tematy:
PET
scintillation crystals
Monte Carlo
lanthanum bromide
Opis:
The main aim of this work is to investigate the use of new lanthanum bromide (LaBr3) in whole body PET (WB-PET) scanners and to compare the obtained results with those for BGO and LSO crystals which are currently used in conventional and dedicated PET systems. Our results show that there is a gain in noise effective count rate (NECR) arising from the reduced scatter and random fractions in a LaBr3 scanner. The spatial resolution of the WB-PET with LaBr3 is slightly worse than the LSO and BGO crystals because of its low density and effective atomic number. But our study showed the increased NECR, excellent energy resolution and low decay time of LaBr3 which promise a significant improvement in WB-PET performances especially for narrow coincidence windows.
Źródło:
Nukleonika; 2008, 53, 1; 5-6
0029-5922
1508-5791
Pojawia się w:
Nukleonika
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Nuclear and mitochondrial genome responses in HeLa cells treated with inhibitors of mitochondrial DNA expression
Autorzy:
Piechota, Janusz
Szczęsny, Roman
Wolanin, Kamila
Chlebowski, Aleksander
Bartnik, Ewa
Powiązania:
https://bibliotekanauki.pl/articles/1041204.pdf
Data publikacji:
2006
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
thiamphenicol
mtDNA depletion
HeLa
dideoxycytidine
mitochondrial biogenesis
ethidium bromide
Opis:
The influence of mutations in the mitochondrial DNA (mtDNA) on the bioenergetic metabolism of the cell is still poorly understood. Many of the mutations in the mtDNA affect the expression of the mitochondrial genome. Investigations on cells from patients are not easy, especially as the mitochondrial DNA is heteroplasmic and this state is changed in culture. Moreover, the nuclear background and the mitochondrial haplotype may affect the behaviour of cells. Transfer of patient mitochondria to rho zero cell lines is also not optimal as these cells in general have many nuclear changes which may also affect cell behaviour. Thus, we decided to use inhibitors of mitochondrial genome expression, such as thiamphenicol, ethidium bromide and dideoxycytidine to investigate the bioenergetic metabolism of HeLa cells. We found that oxidative phosphorylation and glycolysis participate equally in ATP production in HeLa cells and that decreased activity of the respiratory chain leads to increased glycolysis and the reduction of cell growth. Insufficient ATP production in the oxidative phosphorylation process was not compensated by increased proliferation of the mitochondria. However, we were able to show that there are some mechanisms compensating limited expression of the mitochondrial genome within the mitochondria. Experiments with dideoxycytidine revealed that 10-fold decrease of the mtDNA copy number resulted in almost normal activity of cytochrome c oxidase. We found that mtDNA depletion is compensated mostly on the level of RNA metabolism in the mitochondria. Thus, our results are in agreement with the hypothesis that transcription initiation rather than mtDNA copy number is a rate limiting factor for expression of the mitochondrial genome.
Źródło:
Acta Biochimica Polonica; 2006, 53, 3; 485-495
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Investigation of oxidation process of 3-aminotoluene with ozone in presence of manganese bromide catalyst
Issledovanie processa okislenija 3-amino-toluola ozonom v prisutstvii marganec-bromidnogo katalizatora
Autorzy:
Galstyan, A.
Galstyan, G.
Timoshyna, L.
Powiązania:
https://bibliotekanauki.pl/articles/793180.pdf
Data publikacji:
2016
Wydawca:
Komisja Motoryzacji i Energetyki Rolnictwa
Tematy:
oxidation
3-aminotoluene
ozone
sulphuric acid
selectivity
potassium bromide
Źródło:
Teka Komisji Motoryzacji i Energetyki Rolnictwa; 2016, 16, 2
1641-7739
Pojawia się w:
Teka Komisji Motoryzacji i Energetyki Rolnictwa
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Adsorption of Methyl Orange Dye by Modified Fly Ash-Based Geopolymer – Characterization, Performance, Kinetics and Isotherm Studies
Autorzy:
Purbasari, Aprilina
Ariyanti, Dessy
Fitriani, Evi
Powiązania:
https://bibliotekanauki.pl/articles/24201617.pdf
Data publikacji:
2023
Wydawca:
Polskie Towarzystwo Inżynierii Ekologicznej
Tematy:
adsorption
methyl orange
modified fly ash-based geopolymer
CTAB surfactant
cetyltrimethylammonium bromide
Opis:
Geopolymer has been widely used as adsorbent for heavy metals and dyes. Modification on geopolymer surface with cationic surfactant can improve the anion exchange capacity of geopolymer. In this paper, fly ash-based geopolymer had been modified with cetyltrimethylammonium bromide (CTAB) which is cationic surfactant and applied as adsorbent of methyl orange (MO) anionic dye. Modified geopolymer had shown better performance as MO dye adsorbent compared to unmodified geopolymer. The adsorption of MO dye showed the best result at low pH and reached equilibrium after 90 minutes. On the basis of kinetics and isotherm studies, MO dye adsorption by modified geopolymer followed pseudo-second-order model and Langmuir model with maximum adsorption capacity of 19.231 mg∙g-1.
Źródło:
Journal of Ecological Engineering; 2023, 24, 3; 90--98
2299-8993
Pojawia się w:
Journal of Ecological Engineering
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Crystalline structures of Rb2UBr6 ionic conductor determined by neutron diffraction
Autorzy:
Małetka, Krzysztof
Ressouche, Eric
Rundlof, Hakan
Tellgren, Rolland
Szczepaniak, Włodzimierz
Zabłocka-Malicka, Monika
Powiązania:
https://bibliotekanauki.pl/articles/146364.pdf
Data publikacji:
2020
Wydawca:
Instytut Chemii i Techniki Jądrowej
Tematy:
neutron diffraction
Ionic conductivity
solid electrolyte
phase transitions
crystalline structure
uranium bromide
Opis:
The neutron powder diffraction technique has been used for structural studies of Rb2UBr6 solid electrolyte as a function of temperature. The low-, room-, and high-temperature structures have been determined. At the temperature range of 4.2–80 K, the compound crystallizes in a monoclinic unit cell in the P21/c space group. At 80 K and 853 K, the compound crystallizes in a tetragonal unit cell in the P4/mnc space group. At 300 K, the lattice constants are a = b = 7.745(1) and c = 11.064(1) Å. At the temperature range of 853–960 K, a trigonal phase is observed in the P–3ml space group.
Źródło:
Nukleonika; 2020, 65, 1; 3-11
0029-5922
1508-5791
Pojawia się w:
Nukleonika
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Thermo-economic analysis and environmental aspects of absorption refrigeration unit operation onboard marine vehicles: RO- PAX vessel case study
Autorzy:
Ammar, N. R.
Sediek, I. S.
Powiązania:
https://bibliotekanauki.pl/articles/260242.pdf
Data publikacji:
2018
Wydawca:
Politechnika Gdańska. Wydział Inżynierii Mechanicznej i Okrętownictwa
Tematy:
ship emissions
IMO
lithium bromide-water ARU
thermodynamic analysis
economic and environmental analysis
Opis:
Marine diesel engines lose a huge amount of fuel heat content in the form of exhaust gas and jacket cooling water, especially onboard high-powered marine vehicles such as Ro-Pax ships. In this paper, the possibility of using the waste heat of marine diesel engines as a source of heat for air conditioning absorption system is investigated. The thermodynamic analysis, in addition to the environmental and economic analysis of the air condition absorption cycle operated with two heat sources using lithium bromide as absorbent, are performed using the Engineering Equation Solver (EES) software. The last 10 years have seen a steady growth in the passenger ferry and Ro-Pax market, with particularly strong growth in passenger numbers. As a case study, a Ro-Pax vessel operating in the Red Sea area is considered, regarding the profitability of using air conditioning absorption system. The results show specific economic benefits of the jacket cooling water operated absorption refrigeration unit (ARU) over the exhaust gas operated unit, with annual costs of capital money recovery of 51,870 $/year and 54,836 $/year, respectively. Environmentally, applying an ARU machine during cruising will reduce fuel consumption by 104 ton/year. This, in turn, will result in reducing NOx, SOx, and CO2 emissions with cost-effectiveness of 7.73 $/kg, 20.39 $/kg, and 0.13 $/kg, respectively.
Źródło:
Polish Maritime Research; 2018, 3; 94-103
1233-2585
Pojawia się w:
Polish Maritime Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of pH on adsorption and desorption of hexadecyl trimethyl ammonium bromide from silicate surface
Autorzy:
Karaguzel, C.
Xu, Z.
Powiązania:
https://bibliotekanauki.pl/articles/110257.pdf
Data publikacji:
2017
Wydawca:
Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
Tematy:
adsorption
silica sensor
hexadecyl trimethyl ammonium bromide
quartz crystal micro-balance with dissipation monitoring
Opis:
Hexadecyl trimethyl ammonium bromide (HTAB), a cationic surfactant, is physically adsorbed on negatively charged silicate surfaces due to electrostatic forces. This reversible adsorption process is important for surfactant regeneration in some industrial applications such as waste water treatment. Cationic surfactant adsorption and desorption from silica surfaces were studied using several methods such as UV, FTIR, XPS, and XRD. However, most of these methods are time independent and ex situ in nature. Quartz crystal microbalance with dissipation monitoring (QCM-D) is a power tool that can determine the amount and thickness of the deposited material on the surface in situ as a function of time. In this study, the adsorption and desorption characteristic of HTAB was studied on the silica sensor surface by using QCM-D. The adsorption and desorption tests were performed at a constant HTAB concentration (5·10-4 M, close to CMC of HTAB) at different pH values (3, 5.5, 6.5 and 10). The results obtained from these studies clearly showed that pH is a critical factor determining the adsorption and desorption processes.
Źródło:
Physicochemical Problems of Mineral Processing; 2017, 53, 2; 750-757
1643-1049
2084-4735
Pojawia się w:
Physicochemical Problems of Mineral Processing
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Adsorptive performance of MCM-41 towards hg(II) in water. Adsorption and desorption studies
Autorzy:
Zhai, Q. Z.
Hu, W.-H.
Guo, X.-Y.
Powiązania:
https://bibliotekanauki.pl/articles/208154.pdf
Data publikacji:
2016
Wydawca:
Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
Tematy:
electron microscopy
mesoporous silicates
cetyltrimethylammonium bromide
adsorption conditions
mikroskopia elektronowa
krzemiany mezoporowate
bromek cetyltrimethylammonium
warunki adsorpcyjne
Opis:
MCM-41 mesoporous material was prepared under a standard conditions by using cetyltrime-thylammonium bromide as a structure-directing agent and tetraethyl orthosilicate as silica source from the liquid phase at 80 °C. Powder X-ray diffraction and scanning electron microscopy used to characterize the product, showed that the MCM-41material had an average particle size of 110 nm. The synthesized material was used to investigate the effects of acidity, adsorption time, the concentration of Hg2+, adsorbent dosage and temperature on the adsorption of Hg2+. The results showed that the optimal adsorptive conditions were: pH 5.0, m(MCM-41):mHg2+ 6.67, temperature 20 °C and contact adsorption time 60 min, for the initial Hg2+concentration of 1.0 mg/cm3. The maximum adsorptive amount of Hg2+ was 56.48 mg Hg2+/g MCM-41. The results of the desorptive effect of three desorption agents such as HCl, HNO3 and HAc showed that the best desorbent was HCl at the concentration of 0.10 mol/dm3. The highest desorption efficiency was 77.21% for the desorption time 2 h.
Źródło:
Environment Protection Engineering; 2016, 42, 1; 5-15
0324-8828
Pojawia się w:
Environment Protection Engineering
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Research on solvent systems with tetra-n-butylammonium bromide for counter-current chromatography of betalains
Autorzy:
Spórna-Kucab, A.
Wysoczańska, J.
Bartel, A.
Świergosz, T.
Szot, D.
Wybraniec, S.
Powiązania:
https://bibliotekanauki.pl/articles/115657.pdf
Data publikacji:
2016
Wydawca:
Fundacja na Rzecz Młodych Naukowców
Tematy:
betanin
betalains
tetra-n-butylammonium bromide
high-speed counter-current chromatography
Beta vulgaris L.
betanina
betalainy
bromek tetra-n-butyloamoniowy
HSCCC
Opis:
In this study, new two-phase solvent systems for counter-current chromatography (CCC) consisting of n-butanol and water as well as various amounts of acetic acid, acetonitrile, ethanol, acetone or ethyl acetate were tested. Additionally, tetra-n-butylammonium bromide (TBAB) was introduced into the system in the form of aqueous solutions or phosphate-citrate buffer (pH 6.7) in order to form ion-pairs with betalains. The selection of buffer pH was based on their ability to create ion pairs by tetraalkylammonium salts, with selected betalains under these conditions. In this study, it is shown that the settling time of two phases is longer with the increase of acetic acid/acetonitrile/ethanol/acetone/ethyl acetate. For selected solvent systems with high amounts of acetonitrile, ethanol and acetone two phases were not observed. The systems with acetone have the largest increase of settling time. Ethyl acetate systems were characterized by a slow settling time increase. In systems containing additionally 2% aqueous TBAB, smaller changes in settling time than in similar systems without TBAB were observed. Addition of TBAB in the buffer resulted in a prolongation of settling time. Solvent systems in which the separation between the aqueous and organic phases was visually best, were selected from among all the tested systems and the betalain partition coefficients were measured by LC-DAD-ESI-MS. The best results were observed for systems: n-butanol-water-acetic acid (2:2.5:0.75, v/v/v), n-butanol-water-acetic acid (2:2.5:1, v/v/v) and n-butanol-TBAB in water-acetonitrile (2:2.5:0.5, v/v/v).
Źródło:
Challenges of Modern Technology; 2016, 7, 1; 12-16
2082-2863
2353-4419
Pojawia się w:
Challenges of Modern Technology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Modification of the hydrogen bonding network at the reversed micelles interface by near infrared radiation
Autorzy:
Walski, T.
Gałecka, T.
Grzeszczuk-Kuć, K.
Komorowska, M.
Powiązania:
https://bibliotekanauki.pl/articles/148832.pdf
Data publikacji:
2013
Wydawca:
Instytut Chemii i Techniki Jądrowej
Tematy:
electron paramagnetic resonance (EPR)
hydrogen bonds
near infrared radiation (NIR)
cetyltrimethylammonium bromide (TCAB) reverse micelles
TEMPO-palmitate spin probe
water structure modification
Opis:
The purpose of this paper is to prove that near infrared radiation (NIR) modifies hydrogen bonds localized in the interface of reversed micelles. The degree of modification of the hydrogen bonds was monitored by TEMPO-palmitate spin probe introduced into the structure of reverse micelles formed by cetyltrimethylammonium bromide (CTAB) (TCAB/phosphate buffer/isooctane/hexanol and TCAB/NaCl/isooctane/hexanol (W = 15)). Electron paramagnetic resonance (EPR) spectra were performed on the argonated samples. The isotropic tumbling correlation time (τc) and the hyperfine coupling constant A+ = h0 – h+1 (h+1, and h0 correspond to the low-, and centre-field lines, respectively) were determined from the EPR spectra as a quantitative measure for monitoring the action of NIR radiation. A+ values depend on the composition of the water pool (1.640 mT for phosphate buffer and 1.630 mT for NaCl). NIR irradiation led to decrease in A+. This parameter reached the same value for both solutions (1.625 ± 0.003 and 1.626 ± 0.003 mT) after exposition to NIR. The tumbling correlation time after exposure to NIR decreased for TCAB/phosphate buffer/ isooctane/hexanol reversed micelles from 2.10 × 10–10 s to 1.44 × 10–10 s but did not change for TCAB/NaCl/isooctane/ hexanol). The results obtained confirm the possibility of modification of the hydrogen bonds by NIR radiation.
Źródło:
Nukleonika; 2013, 58, 3; 435-438
0029-5922
1508-5791
Pojawia się w:
Nukleonika
Dostawca treści:
Biblioteka Nauki
Artykuł
    Wyświetlanie 1-12 z 12

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