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Wyszukujesz frazę "antisense oligonucleotides" wg kryterium: Temat

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    Wyświetlanie 1-7 z 7
    Tytuł:
    Multidrug resistance-associated protein - reduction of expression in human leukaemia cells by antisense phosphorothioate olignucleotides.
    Autorzy:
    Niewiarowski, Wojciech
    Gendaszewska, Edyta
    Rębowski, Grzegorz
    Wójcik, Marzena
    >Mikołajczyk, Barbara
    Soszyński, Mirosław
    Bartosz, Grzegorz
    Powiązania:
    https://bibliotekanauki.pl/articles/1044273.pdf
    Data publikacji:
    2000
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    antisense oligonucleotides
    MRP
    multidrug resistance
    Opis:
    Multidrug resistance-associated protein (MRP1) causes cellular drug resistance in several cancer cell lines. In this paper we show that antisense oligonucleotides decrease MRP1 expression in human leukaemia cells. We investigated biological activity of a series of 12 linear phosphorothioate oligonucleotides, complementary to several regions of MRP1 mRNA. The oligonucleotides were administered to leukaemia HL60/ADR cells overexpressing MRP1 protein. Then, the level of MRP1 mRNA was determined by means of semiquantitative RT-PCR and the protein level by reaction with specific monoclonal antibodies. Some of the investigated antisense oligonucleotides decrease the expression level of the MRP1 protein by 46% and its mRNA level by 76%.
    Źródło:
    Acta Biochimica Polonica; 2000, 47, 4; 1183-1188
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    RNA modulation, repair and remodeling by splice switching oligonucleotides.
    Autorzy:
    Kole, Ryszard
    Williams, Tiffany
    Cohen, Lisa
    Powiązania:
    https://bibliotekanauki.pl/articles/1043268.pdf
    Data publikacji:
    2004
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    RNA splicing; antisense oligonucleotides; genetic disease
    Opis:
    Targeting splicing by antisense oligonucleotides allows RNA modifications that are not possible with RNA interference or other antisense techniques that destine the RNA for destruction. By changing the ratio of naturally occurring splice variants the expression of mRNA is modulated. By preventing the use of an aberrant splice site created by a mutation and enforcing re-selection of correct splice sites the RNA is repaired. Antisense induced skipping of the exon that carries a nonsense mutation remodels the mRNA and restores the reading frame of the defective protein. All of the above approaches have clinical applications. Modulation of splice variants is particularly important since close to 60% of all genes code for alternatively spliced pre-mRNA.
    Źródło:
    Acta Biochimica Polonica; 2004, 51, 2; 373-378
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Civilization diseases – therapeutic application of antisense strategy
    Autorzy:
    Bąkowska-Żywicka, Kamila
    Tyczewska, Agata
    Twardowski, Tomasz
    Powiązania:
    https://bibliotekanauki.pl/articles/703440.pdf
    Data publikacji:
    2008
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    civilization diseases
    antisense oligonucleotides
    novel therapies
    Opis:
    Modulation and regulation of biosynthesis process by means of antisense oligonucleotides lay the foundation for new therapeutic strategies, among others against cancer, viral or angiogenesis diseases. The aim of this account is the presentation of molecular basis of antisense strategy and mechanism of action of antisense oligonucleotides. Furthermore we present the state of art in clinical studies of application of antisense oligonucleotides in some of civilization diseases.
    Źródło:
    Nauka; 2008, 1
    1231-8515
    Pojawia się w:
    Nauka
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Tissue distribution of a menthyl-conjugated oligodeoxyribonucleotide antisense to PAI-1 mRNA
    Autorzy:
    Szemraj, Janusz
    Al-Nedawi, Khalid
    Chabielska, Ewa
    Buczko, Wlodzimierz
    Pawlowska, Zofia
    Powiązania:
    https://bibliotekanauki.pl/articles/1041329.pdf
    Data publikacji:
    2005
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    PAI-1
    antisense oligonucleotides
    tissue distribution
    Opis:
    The inhibitory effect of numerous analogues of PO-16, an hexadecadeoxyribonucleotide antisense to sequences -22 to -17 of PAI-1 mRNA coding for a fragment of the signal peptide, on the expression of PAI-1 in endothelial cells, and physiological consequences of the subsequently reduced PAI-1 activity tested in vitro and in vivo, were described in our previous studies. Of particular interest was PO-16 5'-O-conjugated with menthyl phosphorothioate (MPO-16R). In this work, tissue localisation of MPO-16R labelled with [35S] phosphorothioate at the 3'-end, was determined. [35S]MPO-16R and control [35S]MPO-16R-SENSE oligonucleotides were administered intravenously into 22 rats and organ distribution of the labelled bioconjugates was assessed after 24 and 48 h. For this purpose, tissue sections were subjected to autoradiography, and quantitated by liquid scintillation after solubilisation. Overall clearance of radioactivity was already seen after 24 h, with the radioactivity recovered mainly in the kidney and liver. A smaller fraction of radioactivity was also retained in the spleen and heart. The kidney concentration of the labelled probe was higher than that of liver by 50%. The distribution of PAI-1 mRNA in untreated rat kidney, liver, spleen and heart established by two independent techniques: Ribonuclease Protection Assay and Real-Time PCR, shows the same pattern as that observed for [35S]MPO-16R antisense.
    Źródło:
    Acta Biochimica Polonica; 2005, 52, 4; 849-855
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    A breakthrough in the efficiency of contact DNA insecticides: rapid high mortality rates in the sap-sucking insects Dynaspidiotus britannicus Comstock and Unaspis euonymi Newstead
    Autorzy:
    Gal`chinsky, N.
    Useinov, R.
    Yatskova, E.
    Laikova, K.
    Novikov, I.
    Gorlov, M.
    Trikoz, N.
    Sharmagiy, A.
    Plugatar, Y.
    Oberemok, V.
    Powiązania:
    https://bibliotekanauki.pl/articles/2082776.pdf
    Data publikacji:
    2020
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    antisense oligonucleotides
    DNA insecticides
    insect pest control
    sap-sucking
    insects
    28S ribosomal RNA
    Opis:
    In this short communication describing experiments carried out on the larvae of two insects, Unaspis euonymi Comstock (feeding on Euonymus japonicus Thunb.) and Dynaspidiotus britannicus Newstead (feeding on Laurus nobilis L.), we evaluate for the first time the efficiency of using DNA insecticides in the control of sap-sucking insects, including armored scale insects. Over a period of 10 days, high insect mortality was detected in both U. euonymi and D. britannicus, accompanied by a significant decrease in the concentration of target RNAs. At the same time, no visible changes were observed when the leaves of the host plants were subjected to treatment with DNA insecticides for one month. The results show the high efficiency of DNA insecticides used against hemipteran insect pests. It is noteworthy that the high efficiency of DNA insecticides and their low cost in comparison with RNA preparations provides a safe and extremely promising potential vehicle for the control of sap-sucking insects.
    Źródło:
    Journal of Plant Protection Research; 2020, 60, 2; 220-223
    1427-4345
    Pojawia się w:
    Journal of Plant Protection Research
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Insecticidal activity of three 10–12 nucleotides long antisense sequences from 5.8S ribosomal RNA gene of gypsy moth Lymantria dispar L. against its larvae
    Autorzy:
    Oberemok, V.V.
    Laikova, K.V.
    Useinov, R.Z.
    Gal`chinsky, N.V.
    Novikov, I.A.
    Yurchenko, K.A.
    Volkov, M.E.
    Gorlov, M.V.
    Brailko, V.A.
    Plugatar
    Powiązania:
    https://bibliotekanauki.pl/articles/2084849.pdf
    Data publikacji:
    2019
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    antisense oligonucleotides
    DNA insecticides
    gypsy moth
    insect pest control
    Lymantria dispar
    5.8S ribosomal RNA
    Opis:
    5.8S ribosomal RNA plays an important role in protein synthesis and eukaryotic ribosome translocation. Contact DNA insecticides based on antisense fragments of 5.8S ribosomal RNA gene of gypsy moth Lymantria dispar L. showed prospective insecticidal activity on its larvae. The most pronounced insecticidal effect was found for antisense fragments 10 and 11 nucleotides long (oligoRIBO-10 and oligoRIBO-11), whereas 12 nucleotides long fragment (oligoRIBO-12) caused the lowest level of insect mortality. This data corresponds to results obtained earlier using rabbit reticulocyte and wheat germ extracts, where maximum inhibition of protein synthesis was observed when a relevant oligomer 10-11 nucleotides long was used, whilst longer chain lengths resulted in reduced inhibition. Using oligoRIBO-11 fragment we have shown penetration of antisense oligonucleotides to insect cells through insects’ exoskeletons. MALDI technique registered the penetration of the oligoRIBO-11 fragment into insect cells after 30 min and a significant response of insect cells to the applied oligonucleotide after 60 min, which indicates not only that the oligonucleotide enters the insect cells, but also the synthesis of new substances in response to the applied DNA fragment. Contact DNA insecticides developed from the L. dispar 5.8S ribosomal RNA gene provide a novel biotechnology for plant protection using unmodified antisense oligonucleotides.
    Źródło:
    Journal of Plant Protection Research; 2019, 59, 4; 561-564
    1427-4345
    Pojawia się w:
    Journal of Plant Protection Research
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Antisense hairpin loop oligonucleotides as inhibitors of expression of multidrug resistance-associated protein 1: Their stability in fetal calf serum and human plasma.
    Autorzy:
    Rębowski, Grzegorz
    Wójcik, Marzena
    Boczkowska, Małgorzata
    Gendaszewska, Edyta
    Soszyński, Mirosław
    Bartosz, Grzegorz
    Niewiarowski, Wojciech
    Powiązania:
    https://bibliotekanauki.pl/articles/1044049.pdf
    Data publikacji:
    2001
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    human plasma
    3' -exonuclease activity
    multidrug resistance-associated protein
    fetal calf serum
    antisense hairpin loop oligonucleotides
    Opis:
    Multidrug resistance-associated protein (MRP1) is a transmembrane pump protein responsible for the efflux of chemotherapeutic drugs, an important cause of anticancer treatment failure. Trying to circumvent MRP-mediated resistance we designed and synthesized hairpin loops forming antisense oligodeoxyribonucleotides (ODNs), both phosphodiesters (PO-ODNs) and their phosphorothioate analogues (PS-ODNs), to reduce the protein expression by targeting its mRNA in a sequence specific manner. Melting temperature measurements as well as polyacrylamide gel electrophoresis supported the preferential formation of a secondary structure, which was expected to protect ODNs against 3'-exonuclease degradation. ODNs and PS-ODNs designed in this work were successfully tested as antisense inhibitors of the expression of MRP1 in the leukaemia HL60/ADR cell line. Foreseeing the necessity to perform clinical studies with such ODNs we investigated their stability against the 3'-exonuclease activity of fetal calf serum and human plasma. Under the conditions, corresponding to physiological ones, we observed high stability of hairpin loop forming ODNs, especially those containing longer (e.g. 7 base pair) stems. Comparative studies on the stability of chemically unmodified hairpin loop forming ODNs and their PS-counterparts indicated that endonuclease activity did not play any important role in the process of their nucleolytic degradation. Our studies provide strong evidence for high stability of chemically unmodified hairpin loop ODNs, making them an attractive alternative to phosphorothioate analogues commonly used in antisense strategy.
    Źródło:
    Acta Biochimica Polonica; 2001, 48, 4; 1061-1076
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-7 z 7

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