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    Wyświetlanie 1-7 z 7
    Tytuł:
    Differentiation by random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) of Candida albicans isolated from upper respiratory tract in patients with non-small cell lung cancer
    Autorzy:
    Biernasiuk, Anna
    Korona-Głowniak, Izabela
    Grzegorczyk, Agnieszka
    Malm, Anna
    Powiązania:
    https://bibliotekanauki.pl/articles/1039202.pdf
    Data publikacji:
    2014
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    RAPD-PCR
    Candida albicans
    upper respiratory tract
    genetic diversity
    Opis:
    Cancer patients are predisposed to fungal infections caused by Candida albicans, especially to oral or respiratory tract candidiasis. The aim of this study was to estimate genetic diversity by RAPD-PCR (random amplified polymorphic DNA-polymerase chain reaction) of C. albicans isolated from upper respiratory tract of 100 patients with non-small cell lung cancer. Among 52 strains, 34 genotypes were defined. 10 clusters comprising 28 (53.85%) isolates with similarity coefficient ≥ 80% were formed. The remaining 24 (46.15%) isolates represented individual genotypes. The RAPD-PCR technique revealed genomic variability within C. albicans isolated from upper respiratory tract of the cancer patients.
    Źródło:
    Acta Biochimica Polonica; 2014, 61, 4; 727-729
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Genetic diversity of F1 and F2 interspecific hybrids between dwarf birch (Betula nana L.) and Himalayan birch (B. utilis var. jacquemontii (Spach) Winkl. 'Doorenbos') using RAPD-PCR markers and ploidy analysis
    Autorzy:
    Czernicka, Małgorzata
    Pławiak, Jarosław
    Muras, Piotr
    Powiązania:
    https://bibliotekanauki.pl/articles/1039274.pdf
    Data publikacji:
    2014
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    Betula nana
    Betula utilis 'Doorenbos'
    interspecific hybrids
    ploidy analysis
    RAPD-PCR
    Opis:
    Crosses between Betula nana and B. utilis 'Doorenbos' were undertaken in order to obtain interspecific hybrids which could be characterized by wide spreading stems, strong branching habit, decorative clear white bark and an interesting shape of purple leaves. The research purpose was to examine genetic diversity of the 16 F1 and F2 putative progenies by using the RAPD-PCR method and the ploidy analysis. A total of 242 RAPD markers were scored with 24 primers and 220 (90.9%) polymorphic bands were found. In the NJ dendrogram, cluster I consisted of the female parent - B. nana and 12 hybrids and cluster II grouped the male parent - B. utilis 'Doorenbos' with 4 hybrids (F2/2, F1/8, F1/7 and F2/1). The 2-D scaling by PCoA was in agreement with the similarity index, i.e. two hybrids (F1/8, F2/2) grouped with the male parent while others with female parent. Classification of the hybrid plants by chromosome counting demonstrated that 13 hybrids were confirmed with accurate chromosome counts as being diploid (2n=2x=28) and 3 plants (F1/7, F1/8, F2/2) as triploid with 42 chromosomes.
    Źródło:
    Acta Biochimica Polonica; 2014, 61, 2; 195-199
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Diversity of Armillaria ostoyae in Scots pine plantations in Poland
    Autorzy:
    Szewczyk, W.
    Kwasna, H.
    Bocianowski, J.
    Behnke-Borowczyk, J.
    Ratajczak, A.
    Swietlik, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/41365.pdf
    Data publikacji:
    2014
    Wydawca:
    Polska Akademia Nauk. Instytut Dendrologii PAN
    Tematy:
    diversity
    Armillaria ostoyae
    Scotch pine
    plantation
    RAPD-PCR method
    rhizomorph
    Polska
    Opis:
    Incidence of Armillaria root disease and the population structure of associated Armillaria spp. were studied in 5-17-year-old Scots pine plantations in west-central Poland. Two infection centres (1.14– 9.30 ha) in each of three forest districts (Siemianice, Zielonka and Złotów) were intensively sampled. Root collars were examined for mycelial fans, decayed wood, and rhizomorphs. Twenty two isolates of Armillaria ostoyae collected from epiphytic rhizomorphs from 20 living and two dead trees in the six infection centres were identified with somatic incompatibility group. Only one somatic incompatibility group for A. ostoyae was found. Twenty one isolates produced rhizomorphs on oak-wood discs submerged in a sand-forest soil substrate. Isolates from Siemianice formed the smallest rhizomorph networks and those from Złotów the most abundant. There were 16 different genets among 22 isolates of A. ostoyae distinguished by RAPD analysis. Genetic similarity among genets was 25.6–97.5%. The large diversity in A. ostoyae suggests that sexual reproduction may occur in nature more often than expected.
    Źródło:
    Dendrobiology; 2014, 72
    1641-1307
    Pojawia się w:
    Dendrobiology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The relationship between RAPD marker-by-marker interactions and quantitative traits of caraway (Carum carvi L.)
    Autorzy:
    Bocianowski, J.
    Seidler-Łożykowska, K.
    Nowosad, K.
    Kuczyńska, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/12681131.pdf
    Data publikacji:
    2019
    Wydawca:
    Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
    Tematy:
    plant cultivation
    caraway
    Carum carvi
    herbal plant
    spice plant
    quantitative trait
    molecular marker
    RAPD marker
    RAPD-PCR reaction
    Opis:
    Application of molecular markers makes the selection process much more effective. Marker assisted selection is an important tool for plant breeders to increase the efficiency of a breeding process, especially for multigenic traits, highly influenced by the environment. Epistasis is the interaction between alleles from two or more loci determining the complex traits, and thus plays an important role in the development of quantitative traits of crops. In this paper, the relationships between RAPD marker-by-marker interactions and 22 quantitative traits of caraway (Carum carvi L.) were analyzed. Significant associations of 116 epistatic markers with at least one trait in 2004 as well as 112 in 2005 were found on the basis of multivariate regression analysis. The proportion of total phenotypic variances of individual trait explained by the marker-by-marker interactions ranged from 25.3% to 96.0%.
    Źródło:
    Acta Scientiarum Polonorum. Hortorum Cultus; 2019, 18, 3; 53-69
    1644-0692
    Pojawia się w:
    Acta Scientiarum Polonorum. Hortorum Cultus
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Malus x oxysepala (M. domestica Borkh. x M. sylvestris Mill.) - a new spontaneous apple hybrid
    Autorzy:
    Czarna, A.
    Nowinska, R.
    Gawronska, B.
    Powiązania:
    https://bibliotekanauki.pl/articles/58374.pdf
    Data publikacji:
    2013
    Wydawca:
    Polskie Towarzystwo Botaniczne
    Tematy:
    Malus x oxysepala
    Malus domestica
    Malus sylvestris
    new hybrid
    spontaneous hybrid
    apple hybrid
    hybrid
    taxonomy
    genetic similarity
    RAPD-PCR method
    Źródło:
    Acta Societatis Botanicorum Poloniae; 2013, 82, 2
    0001-6977
    2083-9480
    Pojawia się w:
    Acta Societatis Botanicorum Poloniae
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Molecular Diagnostic of Streptococcus thermophilus
    Diagnostyka molekularna Streptococcus thermophilus
    Autorzy:
    Vanatkova, Z.
    Okenkova, E.
    Bunkova, L.
    Drab, V.
    Hrabe, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/388393.pdf
    Data publikacji:
    2009
    Wydawca:
    Towarzystwo Chemii i Inżynierii Ekologicznej
    Tematy:
    Streptococcus thermophilus
    PCR
    RAPD
    SDS-PAGE
    Opis:
    Streptococcus thermophilus is one of the most important lactic acid bacteria in the dairy industry. Despite the wide use of Streptococcus thermophilus in the industry, data on the phenotypic and genetic strain variations within the species are still limited. Genetic techniques are very useful for molecular discrimination of complex mixtures of starter and probiotic cultures in research laboratories. Detection and identification of various lactic acid bacteria species with rapid methods is often important for quality control of dairy products. This work deals with characterization and differentiation of strains Streptococcus thermophilus by PCR, RAPD and SDS-PAGE techniques. Fifteen strains of Streptococcus thermophilus from Czech Collection of Dairy Microorganisms (CCDM) and a strain of Streptococcus thermophilus from Czech Collection of Microorganisms (CCM) were used. Particular strains were confirmed with primer set THI/THII by PCR method. Consequently, their identities were examined by RAPD and SDS-PAGE techniques. Whereas, primers OPP-7 and RAPD-4, RAPD were used. It can be claimed that mentioned methods are good means for identification and characterization of streptococci.
    Streptococcus thermofilus jest jednym z najważniejszych przedstawicieli bakterii kwasu mlekowego. Pomimo powszechnego zastosowania tego gatunku w przemyśle mleczarskim nadal nieliczne są dane na temat jego zróżnicowania fenotypowego i genetycznego Streptococcus thermofilus. Szybka identyfikacja różnych gatunków bakterii kwasu mlekowego ma duże znaczenie dla kontroli jakości produktów mleczarskich. Niniejsza praca dotyczy charakterystyk i różnic występujących między różnymi liniami Streptococcus thermofilus. Badania zostały przeprowadzone przy użyciu technik PCR, RAPD i SDS-PAGE. Do badań użyto 15 linii Streptococcus thermofilus z Czeskiego Zbioru Mikroorganizmów Mleczarskich oraz jednej linii Streptococcus thermofilus z Czeskiego Zbioru Mikroorganizmów. Dokonano porównania primerów THI/THII między poszczególnymi liniami bakterii za pomocą techniki PCR. Następnie próbki były badane i identyfikowane przy użyciu technik RAPD i SDS-PAGE. Natomiast primery OPP-7 i RAPD-4 zbadano techniką RAPD. Badania wykazały, że zastosowane techniki mogą być skutecznie wykorzystywane do identyfikacji i charakterystyki bakterii z rodzaju Streptococcus.
    Źródło:
    Ecological Chemistry and Engineering. A; 2009, 16, 12; 1627-1635
    1898-6188
    2084-4530
    Pojawia się w:
    Ecological Chemistry and Engineering. A
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Characteristics of selected molecular methods used in identification and assessment of genetic diversity of bacteria belonging to the genus Azotobacter
    Autorzy:
    Kozieł, Monika
    Gałązka, Anna
    Powiązania:
    https://bibliotekanauki.pl/articles/2147979.pdf
    Data publikacji:
    2019-09-30
    Wydawca:
    Instytut Uprawy Nawożenia i Gleboznawstwa – Państwowy Instytut Badawczy
    Tematy:
    Azotobacter
    ITS PCR
    16S rRNA gen
    PCR MP
    RAPD
    ARDRA
    Opis:
    Modern molecular techniques have greatly increased our knowledge concerning phylogenetic and functional diversity of microorganisms inhabiting the soil environment. Soil ecosys-tem is relatively complex with a high level of microbiologically diversity. The application of traditional culture-based techniques dose not reflect the total diversity of microbial community in-habiting in soil environment. On the other hand commonly used molecular methods allow for quick and accurate identification and evaluation of the genetic diversity of microorganisms in-habiting this environment. Free-living bacteria belonging to the genus Azotobacter commonly occurring in soil. Azotobacter spp. are the subject of many studies conducted both in Poland and in the world. The interest in these bacteria is largely related to their properties very useful for agriculture. Owing to their capability of fixing atmospheric nitrogen and making it available to plants and production of plant growth promotion and fungicidal substances, they are used in the production of soil bacterial inoculants. In addition, these bacteria are an excellent indicator of soil fertil-ity, which is why they are often used as test microorganisms in many studies. The paper presents an overview of molecular mi-crobiological techniques used to identify and evaluate the genetic diversity of Azotobacter spp. in studies conducted both in Poland and across the world. The ITS PCR, PCR-RFLP methods and 16S rRNA gene amplification are used to identify bacteria of the ge-nus Azotobacter, and PCR MP, RAPD PCR and ARDRA are used to assess the genetic diversity of these microorganisms.
    Źródło:
    Polish Journal of Agronomy; 2019, 38; 37-45
    2081-2787
    Pojawia się w:
    Polish Journal of Agronomy
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-7 z 7

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