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Wyszukujesz frazę "Mycoplasma" wg kryterium: Temat


Wyświetlanie 1-6 z 6
Tytuł:
A molecular survey of hemotropic Mycoplasma species in domestic and wild living animals in Slovakia
Autorzy:
Komjati-Nagyova, M.
Víchova, B.
Powiązania:
https://bibliotekanauki.pl/articles/5912.pdf
Data publikacji:
2016
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
molecular survey
Mycoplasma
Mycoplasma ovis
Mycoplasma haemocanis
Mycoplasma haemofelis
Mycoplasma haemobos
domestic animal
wild animal
Slovakia
Źródło:
Annals of Parasitology; 2016, 62, Suppl.
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Preliminary studies on some avian diseases in Antarctic birds
Autorzy:
Leotta, Gerardo
Cerda, Raul
Coria, Nestor R.
Montalti, Diego
Powiązania:
https://bibliotekanauki.pl/articles/2052401.pdf
Data publikacji:
2001
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Antarctica
birds
Mycoplasma
Salmonella
Źródło:
Polish Polar Research; 2001, 22, 3-4; 227-231
0138-0338
2081-8262
Pojawia się w:
Polish Polar Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Laboratory diagnosis of mycoplasma infection in young cattle
Autorzy:
Gabinaitiene, A.
Siugzdaite, J.
Zilinskas, H.
Powiązania:
https://bibliotekanauki.pl/articles/30791.pdf
Data publikacji:
2011
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
mucous membrane
Mycoplasma bovis
Mycoplasma dispar
animal infection
antibody
bacteriology
cattle
diagnosis
histopathology
laboratory diagnosis
mycoplasma
respiratory tract
serology
young animal
isolation
identification
blood sample
blood collecting
Opis:
The aim of this study was to detect Mycoplasma species in the respiratory tract of 110, 310 and 510 day-old groups of cattle by serological, bacteriological and histopathological investigations. Antibodies against M. bovis were found in 75% of the 110 day-old, in 50% – of the 310 day-old and in 55% – of the 510 day-old groups of cattle. Bacteriological examination of the samples from nasal cavities revealed that Mycoplasma carriers were found in 60% of the 110 day-old group of cattle, 40% of the 310 day-old and 40% of the 510 day-old group of cattle. Using the PCR method Mycoplasma was isolated from 25% of lung samples of the 510 day-old group of cattle. Mycoplasma bovis and Mycoplasma dispar were confirmed by serological investigations. Foci of bronchointerstitial pneumonia were determined by histopathological examination in 27.5% of lung samples. Mycoplasma bovis was isolated in 72.7% of bronchointerstitial pneumonia cases. Data processing with an SPSS 13.0 statistical package led to the conclusion that Mycoplasma bovis was found more frequently in the 110 day-old group of cattle (the youngest age group in this study) rather than in the 310 and 510 day-old groups of cattle (χ2 = 6.531; p = 0.038). The results obtained led to the conclusion that serological, bacteriological and histopathological examinations are important in detecting particular animal – carriers of Mycoplasma.
Źródło:
Polish Journal of Veterinary Sciences; 2011, 14, 1
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Expression and immunogenicity study of a novel mhp183 gene fragment of Mycoplasma hyopneumoniae
Autorzy:
Xu, M.
Zheng, J.
Hu, S.
Wang, G.
Powiązania:
https://bibliotekanauki.pl/articles/2087132.pdf
Data publikacji:
2021
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Mycoplasma hyopneumoniae
Mhp183 gene
new generation vaccines
Źródło:
Polish Journal of Veterinary Sciences; 2021, 24, 4; 553-561
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
A new strategy: high level expression and immunogenicity analysis of triplicate repeated multigenes in Mycoplasma hyopneumoniae
Autorzy:
Li, J.
Wang, G.
Powiązania:
https://bibliotekanauki.pl/articles/16647587.pdf
Data publikacji:
2023
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
multi-epitope fusion protein
Mycoplasma hyopneumoniae
new generation vaccines
Opis:
Highly immunogenic nucleotide fragments from 3 genes of Mycoplasma hyopneumoniae strain 232 were selected using information software technology. After repeating each fragment three times, a total of 9 nucleotide fragments were joined together to form a new nucleotide sequence called Mhp2321092bp. Mhp2321092bp was directly synthesized and cloned into a pET100 vector and expressed in Escherichia coli. After purification, the proteins were successfully validated by SDS-PAGE and Western blotting using mouse His-tag antibody and pig anti-Mhp serum. BALB/c mice were intraperitoneally injected with purified proteins in the high-dose (100 µg), medium-dose group (50 µg) and low-dose (10 µg) groups. Mice in each group were injected on day 1, day 8 and day 15 of feeding, respectively. Serum samples were collected from all mice on the day before immunization and on day 22 after immunization. The antibody level in the mouse serum was detected using western blotting using purified expressed proteins as antigens. IL-2, TNF-α and IFN-γ were simultaneously detected in the mouse serum by ELISA. The results showed that the 60 kDa protein was successfully expressed and reacted specifically with the specific serum Mhp His-Tag mouse monoclonal antibody and pig anti-Mhp serum. From day 0 to day 22 of immunization, IFN-γ increased from 269.52 to 467.74 pg/mL, IL-2 increased from 14.03 to 145.16 pg/mL, and TNF-α increased from 6.86 to 12.37 pg/mL. The IgG antibody in mice increased significantly from 0 day to day 22 after immunization. This study suggests that the expressed recombinant protein may serve as one of the novel vaccine candidates for Mhp.
Źródło:
Polish Journal of Veterinary Sciences; 2023, 26, 2; 275-283
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
    Wyświetlanie 1-6 z 6

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