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    Wyświetlanie 1-13 z 13
    Tytuł:
    Ecotoxicity of ionic liquids as lubricants
    Autorzy:
    Kałdoński, T. J.
    Kałdoński, T.
    Załęska-Radziwiłł, M.
    Pawlak, Z.
    Powiązania:
    https://bibliotekanauki.pl/articles/244323.pdf
    Data publikacji:
    2015
    Wydawca:
    Instytut Techniczny Wojsk Lotniczych
    Tematy:
    ionic liquids
    ecotoxicity tests
    Daphnia Magna
    Opis:
    In this article there is discussed the influence of ionic liquids on natural environment on the basis of detailed analysis of the literature information and the results of the Authors' researches carried out within the framework of the development and research project no. PBR/15-249/2007/WAT-OR00002904 financed by Ministry of Science and Higher Education during 2007-2011. The literature information about toxic features of ionic liquids is very skimpy, but the detailed analysis of it and the results of our own researches allow us to say that not all ionic liquids should be treated as fully environmentally friendly substances. Among these substances, there are non-toxic and moderately toxic but there are very toxic liquids, too. Therefore, uncritical rating all ionic liquids among Green Chemistry is inappropriate. The test results of ionic liquids ecotoxicity were compared with the results obtained for standard oils used at lubrication technologies, particularly at motorization. All reference oils, according to EU classification, were non-toxic. The tests were carried out in accordance with OECD 202 European normative document, which recommends carrying out the tests for Daphnia Magna crustaceans. There were carried out: acute immobilization tests to Daphnia Magna, calculation of the effective concentrations EC50-t and evaluation of ionic and reference liquids toxicity with regard to used bioindicators
    Źródło:
    Journal of KONES; 2015, 22, 1; 135-142
    1231-4005
    2354-0133
    Pojawia się w:
    Journal of KONES
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Effects of three pesticides on superoxide dismutase and glutathione-S-transferase activities and reproduction of Daphnia magna
    Autorzy:
    Song, Y.
    Chen, M.
    Zhou, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/204826.pdf
    Data publikacji:
    2017
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    enzyme activity
    reproductive parameter
    pesticide
    Daphnia magna
    Opis:
    Applying pesticides to crops is one of the causes of water pollution by surface runoff, and chlorpyrifos, trifluralin and chlorothalonil are used respectively as insecticide, herbicide and fungicide for crop plants widely. To explore effects of three pesticides on aquatic organisms, superoxide dismutase (SOD) and glutathione S-transferase (GST) activities were determined after 24 h and 48 h exposure of D. magna with ages of 6–24 h to several low concentrations of chlorpyrifos (0.36, 0.72, 1.43, 2.86, 5.72 μg∙L−1), trifluralin (0.17, 0.33, 0.66, 1.33, 2.65 mg∙L−1) and chlorothalonil (0.09, 0.18, 0.36, 0.72, 1.43 mg∙L−1) respectively. Main reproductive parameters including first pregnancy time, first brood time, the number of first brood and total fecundity after 21 d exposures at the same concentrations of pesticides as described above were also measured. The results showed that the activities of GST increased in lower concentrations and decreased in higher concentrations after 24 h exposure to three pesticides, respectively. The activities of SOD showed the same changes after 48 h exposure. With the time prolonged, the activities of GST decreased while the activities of SOD increased. After 21 d exposure, the first pregnancy time and first brood time were delayed, while the number of the first brood and total fecundity per female decreased with increasing concentrations. These results corroborated that GST activity was more sensitive to those pesticides than SOD activity, and there was a significant relationship between total fecundity and pesticides-dose(r>0.94, n=6), GST activity after 48 h exposure and total fecundity after 21 d exposure (r>0.92, n=6).
    Źródło:
    Archives of Environmental Protection; 2017, 43, 1; 80-86
    2083-4772
    2083-4810
    Pojawia się w:
    Archives of Environmental Protection
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Effects of 432 Hz and 440 Hz Sound Frequencies on the Heart Rate, Egg Number and Survival Parameters in Water Flea (Daphnia magna)
    Autorzy:
    Yağcılar, Çetin
    Yardımcı, Mehmet
    Powiązania:
    https://bibliotekanauki.pl/articles/1839638.pdf
    Data publikacji:
    2021
    Wydawca:
    Polskie Towarzystwo Inżynierii Ekologicznej
    Tematy:
    Daphnia magna
    noise pollution
    432 Hz
    440 Hz
    reproduction
    Opis:
    The noise pollution caused by anthropogenic activities in the aquatic environment negatively affects aquatic organisms like mammals, fish, crustaceans, and even plankton, which are important for the aquatic ecosystem. This study was conducted to examine the effect of lethal and sub-lethal effects of high-frequency sound exposure on Daphnia magna aiming to obtain more detailed scientific information. For this purpose, a total of 150 gynandromorphic daphnids were randomly selected, sorted equally into 440 Hz, 432 Hz, and control groups, and high-frequency sounds were applied in aquatic conditions. During the 35-day trial period, the heartbeats, and egg numbers were counted whereas the survival rates were recorded. The results showed that the high-frequency sound exposure immediately showed its detrimental effect on heartbeats after a short adaptation period in the 440 Hz group and the average values decreased to half of the normal values. In the 432 Hz group, heartbeats first increased, and this level was maintained for a while, but then a significant decrease was observed. Regarding the egg numbers, an increasing trend was observed in the 432 Hz group until the 5th measurement period which resulted in a sharp decrease. In the 440 Hz group, lower values with almost no increase were recorded, except for the first measurement period, but no eggs were found in the 55th measurement. Interestingly, while no death was noted during the measurement periods, no living daphnia was observed in the 440 Hz group within 2 weeks during the non-measurement period. As a result, it was understood that Daphnia perceives the vibrations produced by high-frequency sound in the aquatic environment as a stressor and cannot cope with its detrimental effects after a certain time depending on intensity and duration. While the 440 Hz frequency noise caused infertility, the 432 Hz frequency noise resulted in lower egg numbers and heartbeats.
    Źródło:
    Journal of Ecological Engineering; 2021, 22, 4; 119-125
    2299-8993
    Pojawia się w:
    Journal of Ecological Engineering
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Behavioral and Lethal Effects of La Salt and a Mixture of Cu and La Salt on Daphnia magna Straus
    Autorzy:
    Olkova, Anna Sergeewna
    Sysolyatina, Maria
    Powiązania:
    https://bibliotekanauki.pl/articles/2105282.pdf
    Data publikacji:
    2022
    Wydawca:
    Polskie Towarzystwo Inżynierii Ekologicznej
    Tematy:
    lanthanum
    copper
    bioassay
    Daphnia magna
    motor activity
    joint effect
    synergism
    potentiation
    Opis:
    The study of joint effects of REE and HM is relevant, since they are often satellite deposits, their areas of application are similar, and an increase in concentrations of elements of these groups in the areas that are not places of their extraction and enrichment is proven. The purpose of this work was to find out the pre-lethal and lethal effects of La, Cu and their equimolar mixtures in tests for Daphnia magna Straus. Bioassays of artificially polluted natural waters, initially free of toxic elements, was carried out. In bioassays on the mortality of D. magna in the space of 96 hours it was found that acute toxicity of copper sulfate solutions is observed at the calculated concentration of Cu2+ 0.1 mg/L (0.0016 mmol/L), and the acute toxicity of lanthanum sulfate is when the dose of La3+ is equal to 50 mg/L (0.36 mmol/L). In the solutions comprising mixtures of Cu and La salts (1:1 calculated using metals), the concentrations of which are equimolar to the investigated solutions of copper sulfate, the mortality of D. magna begins in the solution containing 10 times less toxic elements. It was found that 25% of individuals died in the variant “0.00016 mmol/L”, the mortality of 100% of individuals was at the total metal concentration of 0.0008 mmol/L. The solutions containing La (0.072–0.72 mmol/L) and Cu (0.00016–0.0016 mmol/L) naturally inhibit the motor activity of D. magna by 1.3–5.3 times and 1.2–1.9 times in 1 hour and 1.7–2.8 and 1.4–2.2 times in 24 hours, respectively. The solutions containing mixtures of Cu and La salts inhibited the motor activity of D. magna in the same way as copper sulfate solutions with the Cu2+ concentrations equimolar “Cu2+ + La3+”. Therefore, when testing the solutions with the same molar concentrations of Cu2+ and the mixture of “Cu2+ + La3+” it was shown that La potentiates the pre-lethal effect of Cu to the level of individual effects of Cu. The additions of La salt to the solutions containing pre-lethal doses of Cu lead to lethal effects of such mixtures for D. magna.
    Źródło:
    Journal of Ecological Engineering; 2022, 23, 6; 245--252
    2299-8993
    Pojawia się w:
    Journal of Ecological Engineering
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    "Battery of Bioassays" for Diagnostics of Toxicity of Natural Water when Pollution with Aluminum Compounds
    Autorzy:
    Olkova, Anna
    Berezin, Grigory
    Powiązania:
    https://bibliotekanauki.pl/articles/1839077.pdf
    Data publikacji:
    2021
    Wydawca:
    Polskie Towarzystwo Inżynierii Ekologicznej
    Tematy:
    bioassay
    aluminum
    battery of bioassay
    Daphnia magna
    Ceriodaphnia affinis
    Paramecium caudatum
    Escherichia coli
    Opis:
    The article presents the results of an experimental comparison of the sensitivity of biotests using Daphnia magna Straus, Ceriodaphnia affinis Lilljeborg, Paramecium caudatum Ehrenberg, and Escherichia coli Migula (strain M-17) to water pollution with aluminum compounds. The research was carried out under simulated conditions: the model toxicant was aluminum sulfate Al2(SO4)3∙18H2O, the concentration range per Al was 0.04–2.8 mg/dm3, and the pH of the tested waters was close to the neutral level of 7.2–7.8. The bioluminescence of E. coli significantly decreased at an Al concentration of 0.8 mg/dm3 (toxicity index was 93.3±1.2, which refers to a high level of toxicity). The reaction of P. caudatum was weaker: a high level of toxicity was achieved at an Al concentration of 2.8 mg/dm3. These doses did not cause the death of D. magna and C. affinis in short-term experiments (28 and 96 hours, respectively). However, in the tests for the chronic toxicity of aluminum, we showed that the doses of 0.8 and 2 mg/dm3 Al cause high death of individuals (more than 50%) and a significant decrease in the number of offspring. The range of sensitivity of the bioassay methods to water pollution with aluminum turned out to be as follows: bioassay for the bioluminescence of E. coli > bioassay for the changes in chemotaxis of P. caudatum > bioassay for the changes in fertility of D. magna > bioassay for the changes in fertility of C affinis.
    Źródło:
    Journal of Ecological Engineering; 2021, 22, 2; 195-199
    2299-8993
    Pojawia się w:
    Journal of Ecological Engineering
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The short-term effects of crude oil on the survival of different size-classes of cladoceran Daphnia magna (Straus, 1820)
    Autorzy:
    Lennuk, L.
    Kotta, J.
    Taits, K.
    Teeveer, K.
    Powiązania:
    https://bibliotekanauki.pl/articles/47987.pdf
    Data publikacji:
    2015
    Wydawca:
    Polska Akademia Nauk. Instytut Oceanologii PAN
    Tematy:
    crude oil
    short-term effect
    pollution
    Daphnia magna
    size class
    survival rate
    aquatic environment
    Źródło:
    Oceanologia; 2015, 57, 1
    0078-3234
    Pojawia się w:
    Oceanologia
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Toxic effect of cyanobacterial blooms on the grazing activity of Daphnia magna Straus
    Autorzy:
    Lotocka, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/48398.pdf
    Data publikacji:
    2001
    Wydawca:
    Polska Akademia Nauk. Instytut Oceanologii PAN
    Tematy:
    Daphnia magna
    Cyanoprokaryota
    Aphanizomenon flos-aquae
    herbivorous organism
    bloom
    grazing activity
    toxic effect
    Microcystis aeruginosa
    Opis:
    The investigations aimed to determine the impact of cyanobacteria Microcystis aeruginosa (K¨utz.) K¨utz. and Aphanizomenon flos-aquae (L.) Ralfs ex Bornet et Flah., both toxic algae, on the grazing intensity of Daphnia magna Straus. In order to determine the parameter permitting the quantitative determination of the grazing intensity of herbivorous organisms, methods based on the following techniques were applied: microscopy, to determine the degree of gut fullness; spectrophotometry, to determine the levels of chlorophyll a and its degradation products in the food composition; high-performance liquid chromatography (HPLC) to determine the content of exogenous and endogenous carotenoids. Each of these methods confirmed that the tested algae species inhibited grazing intensity in D. magna Straus. The most obvious effects were obtained when M. aeruginosa (K¨utz.) K¨utz. was used as food. With these cyanobacteria, the gut fullness indicator did not exceed 58%, and the chlorophyll a content in the digestive system of the tested D. magna was three times lower than that in the control organisms. It seems that the defensive reaction of organisms was a reversible process. However, the possibility of a long-term, sublethal influence of cyanobacteria on the physiology and internal processes of this species cannot be ruled out.
    Źródło:
    Oceanologia; 2001, 43, 4
    0078-3234
    Pojawia się w:
    Oceanologia
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Influence of coagulants used for lake restoration on Daphnia magna Straus (Crustacea, Cladocera)
    Wpływ koagulantów używanych do rekultywacji jezior na Daphnia magna Straus
    Autorzy:
    Piasecki, W.G.
    Zacharzewski, D.
    Powiązania:
    https://bibliotekanauki.pl/articles/85071.pdf
    Data publikacji:
    2010
    Wydawca:
    Akademia Pomorska w Słupsku
    Tematy:
    coagulant
    lake restoration
    Daphnia magna
    Crustacea
    Cladocera
    iron coagulant
    aluminium coagulant
    toxicity
    eutrophication
    technical restoration
    lake condition
    Opis:
    This study examined the influence of ferric and aluminium coagulants (PIX 113 and PAX 18) and ferrous sulphate, used for lake restoration, on growth and mortality of cladocerans Daphnia magna Straus. We observed that addition of these coagulants in concentrations comparable to those used in various restored lakes reduced significantly the increase of Daphnia biomass comparing to the results obtained in the samples without coagulants. This phenomenon was directly proportional to the coagulant concentration. Ferrous sulphate turns out to have been the most toxic compound, while PAX 18 was the less harmful. Ferric coagulants also caused crustacean mortality ranging from 4 to 36%.
    Badano wpływ koagulantów żelazowych i glinowych (PIX 113 i PAX 18) oraz siarczanu żelaza II, używanych do rekultywacji jezior, na wzrost i śmiertelność wioślarki Daphnia magna Straus. Stwierdzono, że dodanie do wody koagulantów w stężeniach porównywalnych ze stosowanymi na jeziorach rekultywowanych powoduje istotny spadek przyrostu biomasy Daphnia w stosunku do wyników otrzymywanych w próbach bez koagulantów. Zjawisko to jest wprost proporcjonalne do stężenia koagulantu. Najbardziej toksyczny okazał się siarczan żelaza II, najmniej – PAX 18. Obecność koagulantów żelazowych powoduje również śmiertelność skorupiaków na poziomie od 4% do 36%.
    Źródło:
    Baltic Coastal Zone. Journal of Ecology and Protection of the Coastline; 2010, 14
    1643-0115
    Pojawia się w:
    Baltic Coastal Zone. Journal of Ecology and Protection of the Coastline
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Predictive acute toxicity comparison in Daphnia magna for common organic chemicals present in cosmetics by using two QSAR modeling softwares
    Autorzy:
    Talapatra, Soumendra Nath
    Konar, Sarnali
    Powiązania:
    https://bibliotekanauki.pl/articles/1190143.pdf
    Data publikacji:
    2016
    Wydawca:
    Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
    Tematy:
    QSAR modelling
    Cosmetic organic chemicals
    T.E.S.T. and ECOSAR software
    Predictive acute toxicity
    Daphnia magna
    Opis:
    Different types of organic compounds, which are used in cosmetics from decades. The usage of cosmetic products are mainly by people in office, culture, festival, recreation etc. All of these chemicals are of synthetic origin. The present study aims to predict comparative acute toxicity as LC50 (median lethal concentration) values in Daphnia magna for common types of cosmetic compounds. The predictions of LC50 values were determined by using two QSAR modeling softwares, T.E.S.T. or Toxicity Estimation Software Tool and ECOSAR (Ecological Structure Activity Relationship), for established 23 types of chemicals commonly present in cosmetics. These two softwares help to predict of LC50 values by easy screening. In present result, predictive data with statistical interpretation (R2 or correlation coefficient values) were obtained easily in T.E.S.T. but not in ECOSAR and the statistical data interpretation was not found. Among all these chemicals, it was observed that major compounds are not toxic to D. magna except few chemicals viz. ascorbyl palmitate, triclosan, methyl triclosan and triclocarbon. Further researches are suggested to compare these predicted data with other available 2-dimentional and 3-dimentional softwares and these chemicals should be studied in combinations of cosmetic chemicals with this test model in vivo because they are major food sources for fish in freshwater ecosystem.
    Źródło:
    World Scientific News; 2016, 42; 101-118
    2392-2192
    Pojawia się w:
    World Scientific News
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Indirect effect of made grounds on the aquatic flora and fauna
    Autorzy:
    Dudziak, M.
    Kopańska, D.
    Powiązania:
    https://bibliotekanauki.pl/articles/207983.pdf
    Data publikacji:
    2017
    Wydawca:
    Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
    Tematy:
    Lemna minor
    Allivibrio fischeri
    Daphnia magna
    aromatic hydrocarbons
    heavy metals
    metallurgical slags
    żużel metalurgiczny
    węglowodory aromatyczne
    metale ciężkie
    Opis:
    The presence of made grounds containing industrial waste materials in the natural environment presents a potential ecological threat. Therefore, in the present study we examined the indirect effects of selected made grounds on the aquatic flora and fauna. The subject of the study were aqueous extracts from made grounds containing metallurgical slags and their effect on the survival of the crustacean daphnia (Daphnia magna) and the inhibition of growth of the plant duckweed (Lemna minor). Due to the fact that duckweed is also used for phytoremediation, the solution after the contact with the plant was assessed for changes of toxicity using the toxicological enzymatic bioassay employing bacteria (Allivibrio fischeri). It was found that the extracts of made grounds adversely affect both the survival of the crustaceans and the growth of plants, however, despite the inhibition of the growth of duckweed it has the ability to phytoremediate contaminants present in the made grounds.
    Źródło:
    Environment Protection Engineering; 2017, 43, 2; 15-24
    0324-8828
    Pojawia się w:
    Environment Protection Engineering
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Effects of ZnO, CuO, Au, and TiO2 nanoparticles on Daphnia magna and early life stages of zebrafish danio rerio
    Autorzy:
    Liu, J.
    Fan, D.
    Wang, L.
    Shi, L.
    Ding, J.
    Chen, Y.
    Shen, S.
    Powiązania:
    https://bibliotekanauki.pl/articles/207169.pdf
    Data publikacji:
    2014
    Wydawca:
    Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
    Tematy:
    metal oxides
    titanium dioxide
    nanoparticles
    toxicity
    zinc oxide
    Daphnia magna
    nanocząsteczki
    tlenki metali
    dwutlenek tytanu
    toksyczność
    tlenek cynku
    rozwielitki
    Opis:
    The effects of four different kinds of nanoparticles (NPs), namely, CuO, ZnO, TiO2, and Au, of the sizes ranging from <20 nm to 50 nm on Daphnia magna, early life stage of zebrafish, and various enzymes have been investigated. The experimental results showed that the NPs inhibited both the body length and hatching rate of zebrafish larvae; the small nanoparticles exhibited more toxicity. In a 21 day chronic toxicity test, metal ions of higher concentrations significantly reduced the number of Daphnia magna offspring. Studies on enzyme activity showed that the NPs reduced the glutathione content and inhibited catalase and superoxide dismutase activities, resulting in shorter body length, lower hatching success, and lower reproduction of zebrafish larvae. Therefore, studies should focus more on the potential toxicity of smaller NPs.
    Źródło:
    Environment Protection Engineering; 2014, 40, 1; 139-149
    0324-8828
    Pojawia się w:
    Environment Protection Engineering
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Responses of the Micro-Crustacean, Daphnia magna, across Five Generations Continuously Exposed to Di-2-Ethylhexyl Phthalate in Mekong River Water
    Reakcje mikroskorupiaków, Daphnia magna, w ciągu pięciu pokoleń stale narażonych na działanie ftalanu di-2-etyloheksylu w wodzie rzeki Mekong
    Autorzy:
    Tran, Vinh-Quang
    Nguyen, Van-Tai
    Dao, Thanh-Son
    Powiązania:
    https://bibliotekanauki.pl/articles/2175329.pdf
    Data publikacji:
    2022
    Wydawca:
    Polskie Towarzystwo Przeróbki Kopalin
    Tematy:
    chronic effects
    Daphnia magna
    energy cost
    life traits
    plastic additives
    skutki chroniczne
    zużycie energii
    cechy życiowe
    dodatki do tworzyw sztucznych
    Opis:
    Plastic pollution has been considered as an emerging environmental problem, and is among the ecological and human health concerns. Detrimental impacts of plastic pollution on living things are closely related to the plastic additives added onto the polymer during plastic manufacture. Di-2-ethylhexyl phthalate (DEHP) is one of the most common plasticizers and is usually found in water environment worldwide. Plastic additives can cause many negative effects on aquatic organisms such as fish and zooplankton. This study aimed to assess the chronic effects of DEHP on the life history traits of an ecotoxicological model micro-crustacean, Daphnia magna, across five generations (F0–F4). We used the natural water from Mekong River in Vietnam as the medium for the D. magna incubation in laboratory conditions. The concentrations of trace elements (e.g., metals and pesticides) in the natural water were under detection levels of equipment or very low which was sufficient for D. magna to grow well. The results showed that the body length was the main endpoint of the organisms inhibited by DEHP across all generations. DEHP adversely impacted the survival and fecundity of D. magna in the fourth generation (F3) only. The adverse effects of DEHP on body length of D. magna should be the consequence of the energy cost and allocation in the exposed organisms. The survival and reproduction responses of D. magna to DEHP across five generations could be explained by (i) the severe effects of the chemical on many individuals in the organism cohort, and (ii) toxin-tolerant development in the remaining exposed organisms. Although the trace elements in natural water from Mekong River were not toxic to D. magna at very low concentrations, together with DEHP they might enhance impacts on the organism. Besides, a multigenerational exposure to DEHP would reflect clearer impacts on the organism than a single exposure. Our results could be useful for extrapolation on the influence of plasticizers on freshwater zooplankton in nature.
    Zanieczyszczenie tworzywami sztucznymi zostało uznane za narastające zagrożenie środowiskowe i jest jednym z problemów ekologicznych związanych ze zdrowiem człowieka. Szkodliwy wpływ zanieczyszczenia tworzywami sztucznymi na organizmy żywe jest ściśle związany z dodatkami tworzyw sztucznych dodawanymi do polimeru podczas produkcji tworzyw sztucznych. Ftalan di2-etyloheksylu (DEHP) jest jednym z najpowszechniejszych plastyfikatorów i zwykle występuje w środowisku wodnym na całym świecie. Dodatki do tworzyw sztucznych mogą powodować wiele negatywnych skutków dla organizmów wodnych, takich jak ryby i zooplankton. Badania przedsatwione w artykule miały na celu ocenę ciągłego wpływu DEHP na cechy historii życia modelu ekotoksykologicznego mikroskorupiaka, Daphnia magna, w ciągu pięciu pokoleń (F0–F4). Jako pożywkę do inkubacji D. magna w warunkach laboratoryjnych wykorzystano naturalną wodę z rzeki Mekong w Wietnamie. Stężenia pierwiastków śladowych (np. metali i pestycydów) w naturalnej wodzie były poniżej poziomu wykrywalności sprzętu lub bardzo niskie, co było wystarczające, aby D. magna dobrze się rozwijała. Wyniki pokazały, że długość ciała była głównym punktem oceny rozwoju organizmów hamowanego przez DEHP we wszystkich pokoleniach. DEHP negatywnie wpłynął na przeżywalność i płodność D. magna jedynie w czwartym pokoleniu (F3). Negatywny wpływ DEHP na długość ciała D. magna powinien być konsekwencją zużycia energii i jej alokacji w narażonych organizmach. Reakcje D. magna na przetrwanie i reprodukcję pod wpływem DEHP w ciągu pięciu pokoleń można wytłumaczyć (i) poważnym wpływem substancji chemicznej na wiele osobników w populacji organizmów oraz (ii) rozwojem tolerancji na toksyny u pozostałych narażonych organizmów. Chociaż pierwiastki śladowe w naturalnej wodzie z Mekongu nie były toksyczne dla D. magna w bardzo niskich stężeniach, to razem z DEHP mogą nasilać oddziaływanie na organizm. Poza tym wielopokoleniowe narażenie na DEHP odzwierciedlałoby wyraźniejszy wpływ na organizm niż jednorazowe narażenie. Przedstawione wyniki mogą być przydatne do ekstrapolacji wpływu plastyfikatorów na zooplankton słodkowodny w przyrodzie.
    Źródło:
    Inżynieria Mineralna; 2022, 2; 77--83
    1640-4920
    Pojawia się w:
    Inżynieria Mineralna
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Application of Biotests in Cyanobacterial Extract Toxicity Assessment
    Zastosowanie biotestów do oceny toksyczności ekstraktu uzyskanego z komórek sinic
    Autorzy:
    Sierosławska, A.
    Rymuszka, A.
    Skowroński, T.
    Powiązania:
    https://bibliotekanauki.pl/articles/204758.pdf
    Data publikacji:
    2014
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    cyanobacterial bloom
    cyanotoxins
    microcystin LR
    anatoxin-a
    biotests
    Daphnia magna
    Thamnocephalus platyurus
    Brachionus calycifl orus
    Tetrahymena thermophila
    cyjanobakteria
    zakwit sinic
    biotest
    mikrocystyna LR
    Daphtoxkit F magna
    Thamnotoxkit F
    Rotoxkit F
    Protoxkit F
    Opis:
    The aim of the study was to determine the toxicity of the extract obtained from the cyanobacterial cells derived from the waters of Zemborzycki dam reservoir with use of a battery of biotests. The taxonomic identification of the bloom-forming cyanobacteria revealed high abundance of Aphanizomenon flos-aquae and Dolichospermum spp. (Anabaena spp.) and in a lower degree of Microcystis aeruginosa and Planktothrix agardhii. In the extract obtained from concentrated cyanobacterial cells, hepatotoxin microcystin-LR at a concentration of 22.89 ± 3.74 μg/L and neurotoxin Antx-a at 13.02 ± 0.01 μg/L have been detected. Toxicity of the extract was evaluated with the following assays: Daphtoxkit F magna with the crustacean Daphnia magna, Thamnotoxkit F with the crustacean Thamnocephalus platyurus, Rotoxkit F with the rotifer Brachionus calyciflorus and Protoxkit F with ciliate Tetrahymena thermophila. The most sensitive organism among all studied was T. platyurus for which EC50 was estimated to be 1.2% of the initial extract concentration. On the basis of the highest obtained value of the toxicity unit (TU = 83) the studied sample was classified to the IV class, which is of high acute toxicity. Additionally, it was found that reactivity on cyanobacterial products differs greatly among organisms used in bioassays, which indicate the need for using a set of biotests.
    Celem pracy była ocena toksyczności ekstraktu uzyskanego z komórek cyjanobakterii obecnych w wodzie pobranej podczas zakwitu sinic z Zalewu Zemborzyckiego, z wykorzystaniem zestawu biotestów. Identyfikacja taksonomiczna wykazała w badanej próbce wysoką liczebność Aphanizomenon fl os-aquae i Dolichospermum spp. (Anabaena spp.) oraz w mniejszym stopniu Microcystis aeruginosa i Planktothrix agardhii. Analiza HPLC ekstraktu uzyskanego z zagęszczonych komórek cyjanobakterii wykazała obecność mikrocystyny-LR w stężeniu 22,89 μg/L oraz anatoksyny-a w stężeniu 13.02 μg/L. Stopień toksyczności uzyskanego ekstraktu oceniano na podstawie reakcji organizmów testowych, z użyciem następujących biotestów: Daphtoxkit F magna (Daphnia magna), Thamnotoxkit F (Thamnocephalus platyurus), Rotoxkit F (Brachionus calyciflorus) oraz Protoxkit F (Tetrahymena thermophila) (Microbiotests Inc., Belgia). Przeprowadzone badania wykazały szczególnie silne działanie toksyczne analizowanego ekstraktu wobec T. platyurus (24hEC50 oszacowane na 1,2% ekstraktu wyjściowego). Wyraźny efekt toksyczny obserwowany był także wobec B. calyciflorus i w mniejszym stopniu wobec T. thermophila. Natomiast największą odporność na toksyczne działanie ekstraktu wykazywała D. magna. Na podstawie uzyskanych wartości TU (jednostek toksyczności) badany ekstrakt zaliczony został do IV klasy toksyczności, tj. o wysokiej toksyczności ostrej. Z przeprowadzonych badań wynika, że organizmy na których wykonywane są testy toksyczności wykazują zróżnicowaną wrażliwość na produkty cyjanobakterii, stąd ocena toksyczności próbek zawierających tego typu substancje powinna być prowadzona z wykorzystaniem jak najszerszego zestawu biotestów.
    Źródło:
    Archives of Environmental Protection; 2014, 40, 3; 115-121
    2083-4772
    2083-4810
    Pojawia się w:
    Archives of Environmental Protection
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
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