Temat: DNA isolation - Katalog OPAC zbiorów

Skocz do pozycji: 1.

Tytuł:: Isolation of DNA from bone material of selected animals from Cervidae family
Autorzy:: Pizoń, Klaudia
Powiązania:: https://bibliotekanauki.pl/articles/1178691.pdf
Data publikacji:: 2017
Wydawca:: Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:: DNA isolation
bone material
Opis:: Bone material is one of the most difficult biological samples used for extraction of DNA. The primary objective of molecular tests is to determine the concentration and quality of DNA, and these results are of significant importance in further analyses. The aim of the study was to evaluate the usefulness of a method for DNA isolation from bone material of selected animal species from Cervidae family using a commercially available GeneMatrix Bone DNA Purification Kit manufactured by Eurx. Quantitative evaluation was performed with a spectrophotometer. Pure DNA isolates were obtained from 21 out of 24 bone fragments. The efficiency of the applied isolation method varied. The difference between the lowest and highest DNA concentration was more than hundredfold. Qualitative analysis was carried out by means of electrophoresis in 1% agarose gel. No high molecular mass DNA was obtained. The genetic material was present in small quantity and it was fragmented. It was concluded that the Bone DNA Purification Kit manufactured by Eurx may be effective only in isolation of DNA from bones stored in good conditions.
Źródło:: World Scientific News; 2017, 67, 2; 265-276
2392-2192
Pojawia się w:: World Scientific News
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 2.

Tytuł:: Molecular identification of Trichoderma strains collected to develop plant growth-promoting and biocontrol agents
Autorzy:: Oskiera, M.
Szczech, M.
Bartoszewski, G.
Powiązania:: https://bibliotekanauki.pl/articles/1976.pdf
Data publikacji:: 2015
Wydawca:: Instytut Ogrodnictwa
Tematy:: Trichoderma
strain
molecular identification
biological control agent
multilocus sequence typing
phylogenesis
species identification
DNA isolation
Źródło:: Journal of Horticultural Research; 2015, 23, 1
2300-5009
Pojawia się w:: Journal of Horticultural Research
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 3.

Tytuł:: Molecular studies in osteogenesis imperfecta [OI] II. Evaluation of intragenic polymorphic sites in COL1A1 and COL1A2 loci
Autorzy:: Kostyk, E
Sucharski, P.
Pietrzyk, J.J.
Kruczek, A.
Powiązania:: https://bibliotekanauki.pl/articles/2044212.pdf
Data publikacji:: 1998
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: intragenic polymorphic site
polymorphism
haplotype
DNA isolation
COL1A1 gene
electrophoresis
collagen
COL1A2 gene
molecular marker
osteogenesis imperfecta
Opis:: The goal of the study was to evaluate intragenic polymorphic sites in COL1A1 and COL1A2 loci. For COL1A1 the following intragenic markers were used: PCR-RFLP (COL1A1), G/A polymorphism in exon 45 of COL1A1 and C/T polymorphism in +88 position of COL1A1 non-translatable 3’ end. For COL1A2 PCR-VNTR was analyzed. 17 families were examined (6 of the "simplex" type and 11 of the "multiple" type). In 8 out of 11 "multiplex" families the segregation of the markers revealed correlation with OI, whereas the other 3 were non-informative. The method was not useful in "simplex" families.
Źródło:: Journal of Applied Genetics; 1998, 39, 4; 349-365
1234-1983
Pojawia się w:: Journal of Applied Genetics
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 4.

Tytuł:: DNA isolation method from human blood with MasterPure DNA Purification Kit™ – review article
Autorzy:: Florczak, Sylwia
Kempka, Katarzyna
Hołderna, Karolina
Stanek, Emilia
Baszyński, Jędrzej
Kamiński, Piotr
Bogdzińska, Maria
Powiązania:: https://bibliotekanauki.pl/articles/1179641.pdf
Data publikacji:: 2017
Wydawca:: Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:: DNA
human
isolation
optimization
review
whole blood
Opis:: The main element of genomic molecular analysis is isolation of DNA from biological material. DNA isolation is an important process of PCR methods. Efficency of PCR processes depends of purness and concentration of DNA. Many methods allows to izolate good quality DNA. There are many Purification Kits and methods of extraction genomic material. We show available techniques and methods of increasing efficiency of the process in our studies. Previous data shows that operating with kits needs optimization, whilst protocols do not have all information about isolation process. We concentrate on Epicentre MasterPure DNA Purification Kit, which is cheap, fast and allows to purification nucleic acids from various materials. We show detailed advantages and disadvantages of this set. Our main aim is to develop protocol to get optimal concentration (50-100 ng/μL) and pureness (A 260/280; 1.6-1.9) of DNA. We also should optimize the process of DNA isolation to obtain efficiency of about 100%. Optimized methodology of DNA isolation, with using MasterPure DNA Purification Kit allows to get good quality material in laboratory without specialistic equipment. This survey contains our assumptions of ways how we can influent on purification process according to previous data.
Źródło:: World Scientific News; 2017, 72; 69-76
2392-2192
Pojawia się w:: World Scientific News
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 5.

Tytuł:: Virulence and antibiotic resistance of Escherichia coli isolated from rooks
Autorzy:: Kmet, V.
Drugdova, Z.
Kmetova, M.
Stanko, M.
Powiązania:: https://bibliotekanauki.pl/articles/51141.pdf
Data publikacji:: 2013
Wydawca:: Instytut Medycyny Wsi
Tematy:: virulence
antibiotic resistance
Escherichia coli
isolation
rook
polymerase chain reaction
DNA microarray
Opis:: With regard to antibiotic resistance studies in various model animals in the urban environment, the presented study focused on the rook, many behavioural and ecological aspects of which are important from an epidemiological point of view. A total of 130 Escherichia coli strains isolated from rook faeces during a two-year period (2011–2012) were investigated for antibiotic resistance and virulence. Resistance to ampicillin (60%) and streptomycin (40%) were the most frequent, followed by resistance to fluoroquinolones (ciprofloxacin-22% and enrofloxacin-24%), tetracycline (18%), cotrimoxazol (17%) and florfenicol (14%). Ceftiofur resistance occured in 10.7 % of strains and cefquinom resistance in 1.5 % of strains. Twenty-five E.coli strains with a higher level of MICs of cephalosporins (over 2mg/L of ceftazidime and ceftriaxon) and fluoroquinolones were selected for detection of betalactamase genes (CTX-M, CMY), plasmid-mediated quinolone resistance qnrS, integrase 1, and for APEC (avian pathogenic E.coli) virulence factors (iutA, cvaC, iss, tsh, ibeA, papC, kpsII). Genes of CTX-M1, CMY-2, integrase 1, papC, cvaC, iutA were detected in one strain of E.coli, and qnrS, integrase 1, iss, cvaC, tsh were detected in another E.coli. DNA microarray revealed the absence of verotoxin and enterotoxin genes and pathogenicity islands. The results show that rooks can serve as a reservoir of antibiotic-resistant E. coli with avian pathogenic virulence factors for the human population, and potentially transmit such E.coli over long distances.
Źródło:: Annals of Agricultural and Environmental Medicine; 2013, 20, 2
1232-1966
Pojawia się w:: Annals of Agricultural and Environmental Medicine
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 6.

Tytuł:: Cloning of the DNA fragments reflecting the open reading frame I and II of the I-18 C gene of Chironomus tentans. I. Preparation of the bacterial cells, transformation and isolation of the bluescript plasmid
Autorzy:: Borowicz, B P
Powiązania:: https://bibliotekanauki.pl/articles/66849.pdf
Data publikacji:: 1999
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: transformation
promoter system
gene expression
cloning
I-18 C gene
isolation
T7 RNA polymerase
plasmid
Chironomus tentans
bacterial cell
polymerase chain reaction
DNA fragment
Opis:: The technology applied for the cloning of the open reading frame (ORF) I and II reflecting DNA fragments of the I-18 C gene of Chironomus tentans in regard to the preparation of the competent E. coli cells of the XL-1 strain needed for the transformation with the ligation reaction products, is presented. Also the transformation of these bacterial cells with the bluescript plasmid and its isolation for these cloning experiments, are described.
Opisano technologię, którą zastosowano do klonowania fragmentów DNA odzwierciedlających translacyjną otwartą ramę odczytu I i II genu I-18 C Chironomus tentans w zakresie: przygotowania kompetentnych komórek E. coli szczepu XL-1, poddawanych następnie transformacji produktami reakcji ligacji. Przedstawiono także zastosowaną technologię transformacji wymienionych komórek bakteryjnych przy użyciu plazmidu - blueskrypt oraz izolację tego plazmidu, w celu jego zastosowania w wymienionych eksperymentach klonowania.
Źródło:: Journal of Plant Protection Research; 1999, 39, 1
1427-4345
Pojawia się w:: Journal of Plant Protection Research
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 7.

Tytuł:: Molecular studies in osteogenesis imperfecta [OI] III. cDNA of COL1A1 and COL1A2 analysis using the BESS-T-Scan technique
Autorzy:: Sucharski, P
Sanak, M.
Kostyk, E.
Pietrzyk, J.J.
Kruczek, A.
Powiązania:: https://bibliotekanauki.pl/articles/2044221.pdf
Data publikacji:: 1998
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: COL1A1 gene
electrophoresis
COL1A2 gene
collagen production
man
RNA isolation
mutation
BESS-T-Scan technique
molecular diagnosis
DNA
osteogenesis imperfecta
cDNA synthesis
fibroblast culture
Opis:: A BESS-T-Scan analysis of cDNA COL1A1 and COL1A2 obtained by RT-PCR derived from five patients with sporadic forms of ostegenesis imperfecta was performed. The study was done in four patients with type I and one patient with type III OI. The analysis revealed the presence of structural changes in two regions of cDNA COL1A1 in two patients. No quantitative changes referring to COL1A2 gene were noted in any patient. The above analysis was the first application of the BESS-T-Scan technique in a molecular diagnosis of OI. The applied method seems to be useful and fulfil the basic criteria of the screening method to detect and locate mutations.
Źródło:: Journal of Applied Genetics; 1998, 39, 4; 367-373
1234-1983
Pojawia się w:: Journal of Applied Genetics
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 8.

Tytuł:: Applications of recent advances at the Institute of Grassland and Environmental Research in cytogenetics of the Lolium-Festuca complex
Autorzy:: Humphreys, M W
Thomas, H M
King, I P
Morgan, W G
Meredith, M R
Harper, J A
Humphreys, M O
Bettany, A J E
Dalton, S J
James, A R
Ougham, H J
Thomas, H
Powiązania:: https://bibliotekanauki.pl/articles/2046675.pdf
Data publikacji:: 1997
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: RNA
in situ
tissue culture
gene transfer
chromosome segment
anther culture
androgenesis
plant breeding
introgression mapping
fluorescence
hybridization
Lolium-Festuca complex
drought resistance
gene isolation
tetraploid hybrid
DNA
meiosis
grass
chromosome behaviour
Opis:: Recent advances at Institute of Grassland and Environmental Research (Aberystwyth, U.K.) in cytogenetics of the Lolium/Festuca complex places us in the advantageous position of being able to map genes of agronomic importance onto chromosome arms using fluorescence in situ hybridization (FISH). The ability to physically map genes leads to the capability for "dissecting" quantitative traits into their different components and will lead to better understanding of the complex physiological processes involved and the identification of their genetic control. By tagging genes of interest, using molecular and morphological markers, it will be possible to select and combine suites of desirable genes in a single genotype and thus produce novel cultivars by conventional breeding procedures. Programmes for introgression depend on the relationships between species and on levels of chromosome pairing. Phylogenetic relationships within the Lolium/Festuca complex are being determined using both genomic in situ hybridization (GISH) and FISH. With recent advances in genetic manipulation within the Lolium/Festuca complex, opportunities now arise for gene transfer from Lolium and Festuca species into other important agricultural crops.
Źródło:: Journal of Applied Genetics; 1997, 38, 3; 273-284
1234-1983
Pojawia się w:: Journal of Applied Genetics
Dostawca treści:: Biblioteka Nauki

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