Temat: Bioimaging - Katalog OPAC zbiorów

Skocz do pozycji: 1.

Tytuł:: A coumarin-based fluorescent probe for specific and rapid detection of fluoride ions
Autorzy:: Xin, Z.-H.
Meng, Y.-L.
Wu, Y.-H.
Feng, Z.-K.
Kang, Y.-F.
Powiązania:: https://bibliotekanauki.pl/articles/778907.pdf
Data publikacji:: 2018
Wydawca:: Zachodniopomorski Uniwersytet Technologiczny w Szczecinie. Wydawnictwo Uczelniane ZUT w Szczecinie
Tematy:: Fluorion
Fluorescence
Coumarin
Probe
Bioimaging
Opis:: A simple and coumarin-based fluorescence probe has been designed and synthesized with silyl group as recognition group of fluoride ions (F−) in this study. The results showed that the fluorescence intensity of the probe displayed prominent enhancement with addition of F− at 445 nm with incubation of 1 min. There was an excellent linear relationship between fluorescence intensity and fluoride concentration from 0 to 30 μM (0~0.57 ppm), which offered the important condition for the quantitative analysis. In addition, the highly selective response to fluorion, the low detection limit with 28 nM (0.532 ppb), low toxicity and bioimaging afforded an advantage for practical application and detecting fluoride in biological systerms.
Źródło:: Polish Journal of Chemical Technology; 2018, 20, 4; 1-5
1509-8117
1899-4741
Pojawia się w:: Polish Journal of Chemical Technology
Dostawca treści:: Biblioteka Nauki

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Tytuł:: The use of LA-ICP-MS as an auxiliary tool to assess the pulmonary toxicity of molybdenum(IV) sulfide (MoS₂) nano- and microparticles
Autorzy:: Kuraś, Renata
Stępnik, Maciej
Domeradzka-Gajda, Katarzyna
Janasik, Beata
Powiązania:: https://bibliotekanauki.pl/articles/28761978.pdf
Data publikacji:: 2024-03-05
Wydawca:: Instytut Medycyny Pracy im. prof. dra Jerzego Nofera w Łodzi
Tematy:: microparticles
LA-ICP-MS
molybdenum(IV) disulfide
bioimaging
rat tissues
nanoparticles
Opis:: Objectives Laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) has considerable applicative potential for both qualitative and quantitative analyses of elemental spatial distribution and concentration. It provides high resolutions at pg-level detection limits. These qualities make it very useful for analyzing biological samples. The present study responds to the growing demand for adequate analytical methods which would allow to assess the distribution of nanostructured molybdenum(IV) disulfide (MoS₂) in organs. It was also motivated by an apparent lack of literature on the biological effects of MoS₂ in living organisms. The study was aimed at using LA-ICP-MS for comparing micro- and nanosized MoS₂ ditribution in selected rat tissue samples (lung, liver, brain and spleen tissues) after the intratracheal instillation (7 administrations) of MoS₂ nano- and microparticles vs. controls. Material and Methods The experimental study, approved by the Ethics Committee for Animal Experiments was performed using albino Wistar rats. This was performed at 2-week intervals at a dose of 5 mg/kg b.w., followed by an analysis after 90 days of exposure. The MoS₂ levels in control tissues were determined with the laser ablation system at optimized operating conditions. The parameter optimization process for the LA system was conducted using The National Institute of Standards and Technology (NIST) glass standard reference materials. Results Instrument parameters were optimized. The study found that molybdenum (Mo) levels in the lungs of microparticle-exposed rats were higher compared to nanoparticle-exposed rats. The opposite results were found for liver and spleen tissues. Brain Mo concentrations were below the detection limit. Conclusions The LA-ICP-MS technique may be used as an important tool for visualizing the distribution of Mo on the surface of soft samples through quantitative and qualitative elemental mapping.
Źródło:: International Journal of Occupational Medicine and Environmental Health; 2024, 37, 1; 18-33
1232-1087
1896-494X
Pojawia się w:: International Journal of Occupational Medicine and Environmental Health
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 3.

Tytuł:: Machine learning system for automated blood smear analysis
Autorzy:: Grochowski, Michał
Wąsowicz, Michał
Mikołajczyk, Agnieszka
Ficek, Mateusz
Kulka, Marek
Wróbel, Maciej S.
Jędrzejewska-Szczerska, Małgorzata
Powiązania:: https://bibliotekanauki.pl/articles/220750.pdf
Data publikacji:: 2019
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: optical microscopy
blood cells
biophotonics
image analysis
classification
eigenfaces
neural networks
decision support
nanodiamonds
bioimaging
Opis:: In this paper the authors propose a decision support system for automatic blood smear analysis based onmicroscopic images. The images are pre-processed in order to remove irrelevant elements and to enhancethe most important ones – the healthy blood cells (erythrocytes) and the pathologic ones (echinocytes). The separated blood cells are analysed in terms of their most important features by the eigenfaces method. The features are the basis for designing the neural network classifier, learned to distinguish between erythrocytes and echinocytes. As the result, the proposed system is able to analyse the smear blood images in a fully automatic way and to deliver information on the number and statistics of the red blood cells, both healthy and pathologic. The system was examined in two case studies, involving the canine and human blood, and then consulted with the experienced medicine specialists. The accuracy of classification of red blood cells into erythrocytes and echinocytes reaches 96%.
Źródło:: Metrology and Measurement Systems; 2019, 26, 1; 81-93
0860-8229
Pojawia się w:: Metrology and Measurement Systems
Dostawca treści:: Biblioteka Nauki

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