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    Wyświetlanie 1-4 z 4
    Tytuł:
    Evaluation of the activity of thermostable DNA polymerases in the presence of heme, as a key inhibitor in the real time PCR method in diagnostics of sepsis
    Autorzy:
    Gosiewski, Tomasz
    Brzychczy-Włoch, Monika
    Pietrzyk, Agata
    Sroka, Agnieszka
    Bulanda, Małgorzata
    Powiązania:
    https://bibliotekanauki.pl/articles/1039451.pdf
    Data publikacji:
    2013
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    polymerase inhibitor
    heme
    real time PCR
    sepsis
    Opis:
    The study aim was evaluation of the usefulness of several thermostable DNA polymerases in real time PCR conducted in the presence of the heme. Our study had the advantage of testing several different polymerases, one of which proved to be the least sensitive to heme activity. We also found that there is no need of supplementing the reaction mixture with protective substances like BSA. Selection of the appropriate polymerase can increase the efficiency of the PCR reaction which is very important for diagnosis of sepsis and for other analyses performed on DNA template isolated from the blood.
    Źródło:
    Acta Biochimica Polonica; 2013, 60, 4; 603-606
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    University Students’ vs. Lay People’s Perspectives on Organ Donation and Improving Health Communication in Poland
    Autorzy:
    Boratyński, Wojciech
    Bączek, Grażyna
    Dyzmann-Sroka, Agnieszka
    Jędrzejczak, Agnieszka
    Kielan, Aleksandra
    Mularczyk-Tomczewska, Paulina
    Nowak, Ewa
    Steć, Małgorzata
    Szynkiewicz, Mariusz
    Powiązania:
    https://bibliotekanauki.pl/articles/781215.pdf
    Data publikacji:
    2018
    Wydawca:
    Uniwersytet im. Adama Mickiewicza w Poznaniu
    Tematy:
    organ donation
    societal assessment
    transplant policies in Poland
    promoting decision making
    health communication and discourse in university education
    Opis:
    Given that organ transplant is a standard medical technology admitted in medical practice, and taking into consideration that Polish transplantology is regarded among the most advanced in the world one should expect to find similarly high levels of acceptance in interviewees asked for their opinion on vital organ transplantation and their willingness to donate a paired organ ex vivo, or a vital organ ex mortuo in order to rescue the life of a recipient with a missing vital organ. The paper presents research build on the societal assessment of vital organ donation and transplant policies in Poland with the focus on students. Data have been collected at three different universities (Boratyński et al., Questionnaire on the Bases of Transplantation Medicine 2016/7). Various assessments concerning a vital organ donation have been observed. The authors discuss educational factors contributing to these variety including factual knowledge and ethical issues.
    Źródło:
    Ethics in Progress; 2018, 9, 2; 99-117
    2084-9257
    Pojawia się w:
    Ethics in Progress
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Impact of biological treatment on intestinal microbiom in children with Crohn’s disease
    Autorzy:
    Krawczyk, Agnieszka
    Sroka-Oleksiak, Agnieszka
    Kowalska-Duplaga, Kinga
    Fyderek, Krzysztof
    Gosiewski, Tomasz
    Salamon, Dominika
    Powiązania:
    https://bibliotekanauki.pl/articles/1177355.pdf
    Data publikacji:
    2018
    Wydawca:
    Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
    Tematy:
    Crohn’s disease
    biological treatment
    children
    gut microbiota
    Opis:
    Crohn’s disease (CD) is a chronic, inflammatory illness of the digestive tract, characterized by alternating periods of remission and recurrence. The pathogenesis of CD is still unclear but probably is a result of a complex interaction between immunological, genetic and microbiological disorders. In recent years, there has been an increasing extent of evidence that gut microbiota plays a very important role in the pathogenesis of CD. Currently, the most effective treatment is biological therapy using anti-TNF monoclonal antibodies. It is interesting whether biological drugs resulting in fast remission, contributes to the normalization of the gut microbiota. Due to the fact that the children’s population is a significant percentage of all patients with CD, it is important to pay close attention to the problem of microbiological disorders in this age group. The aim of this study was to investigate whether there are quantitative changes of chosen bacteria species and fungi of the genus Candida in children with Crohn's disease relative to healthy children and assesment of quantitative changes in patients after biological treatment. In the group of children with Crohn’s disease, the numbers in Candida were significantly higher (9.74×1017 CFU/g) than in the control group (9.35×1010 CFU/g, p = 0.011). Biological therapy led to a significant reduction in the amount Candida (5.91×1011) and was comparable with the number in the control group. In the case of bacteria, we observed an increase in S. marcescens (3,4×108) in the patients group compared to the controls (1,85×108) and an increase in L. fermentum (2,34×1010) in relation to healthy children (3,31×108, p = 0,048) Biological treatment had an impact on the decrease in L. fermentum (4,76×109, p = 0.05).
    Źródło:
    World Scientific News; 2018, 104; 245-256
    2392-2192
    Pojawia się w:
    World Scientific News
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Social councils and committees as (not quite) present in local decision-making processes
    Społeczne rady i komisje jako (nie)obecny uczestnik lokalnego procesu decyzyjnego
    Autorzy:
    Pawłowska, Agnieszka
    Kmieciak, Robert
    Kołomycew, Anna
    Radzik-Maruszak, Katarzyna
    Antkowiak, Paweł
    Wójcik, Bartosz
    Sroka, Jacek
    Wojnicki, Jacek
    Powiązania:
    https://bibliotekanauki.pl/books/19233495.pdf
    Data publikacji:
    2021
    Wydawca:
    Wydawnictwo Naukowe Scholar
    Opis:
    Social councils and committees (SCs), the topic of this volume, cannot yet be called a well-researched matter, and – whether in Poland or internationally – very few analyses have focused on them, or have done so in a more than fragmentary fashion. Although they constitute a challenging subject, among other things due to their sometimes non-obligatory character, term limits, the availability of members, and diversity, we believe they deserve academic interest as well as the attention of the public and the third sector. SCs have the potential to become an important actor of the local decision-making process. This thread was the crucial element of our research, whose aim was primarily to define the impact of SCs on decision-making, as well as to describe the character of these entities, which in our view show features of participatory and deliberative solutions. Another major objective of our research was to identify the advantages of the participation of these bodies in the local decision-making process as well as to indicate the factors that hinder or prevent their potential as the participants of this process. From the Introduction
    Dostawca treści:
    Biblioteka Nauki
    Książka
      Wyświetlanie 1-4 z 4

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