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    Wyświetlanie 1-6 z 6
    Tytuł:
    Analysis of integrins and vascular endothelial growth factor isoforms mRNA expression in the canine uterus during perimplantation period
    Autorzy:
    Bukowska, D.
    Kempisty, B.
    Jackowska, M.
    Wozna, M.
    Antosik, P.
    Piotrowska, H.
    Jaskowski, J.M.
    Powiązania:
    https://bibliotekanauki.pl/articles/31799.pdf
    Data publikacji:
    2011
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    integrin
    vascular endothelial growth factor
    isoform
    mRNA expression
    dog
    uterus
    pregnancy
    bitch
    Opis:
    Integrins are the major receptors within the extracellular matrix (ECM) that mediate several functions connected with cell life and metabolism, such as cell adhesion, migration, cytoskeletal organization, proliferation, survival, and differentiation. A vascular endothelial growth factor (VEGF) is one of the most important angiogenic factors. It has been suggested that the expression of this gene may play crucial physiological roles in reproductive organs. All investigated endometrial tissues were isolated on day 10-12 after mating. Control bitches, used in this study, were in metestrus, which was determined according to the vaginal cytology and progesterone level in blood. Early pregnancy was verified by flushing the uterine horns with PBS. Total RNA was isolated from the bitches endometrium by means of the Chomczyński and Sacchi method, treated by DNase I, and reverse-transcribed into cDNA. A quantitative analysis of integrins α2b, β2 and β3, VEGF 164, 182 and 188 cDNA was performed by RT-PCR. In results we have shown an increased expression of all investigated genes (integrins α2b, β2 and β3, VEGF 164, 182, and 188) in pregnant bitches uterus as compared to non-pregnant females (P<0.001). Our results indicated that the expression of genes encoding integrins and vascular endothelial growth factors is different in relation to the time of the embryo implantation and it is increased in the first period of this process. This may be associated with the induction of specific mechanisms responsible for receptivity of uterus following the embryo attachment. In addition, all of investigated genes are up-regulated in a pregnancy-specific manner and the increased expression of these genes may regulate the uterus function during the implantation of canine embryos.
    Źródło:
    Polish Journal of Veterinary Sciences; 2011, 14, 2
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Differential expression of epidermal growth factor and transforming growth factor beta isoforms in dog endometrium during different periods of the estrus cycle
    Autorzy:
    Bukowska, D.
    Kempisty, B.
    Jackowska, M.
    Wozna, M.
    Antosik, P.
    Piotrowska, H.
    Jaskowski, J.M.
    Powiązania:
    https://bibliotekanauki.pl/articles/30107.pdf
    Data publikacji:
    2011
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    expression
    epidermal growth factor
    transforming growth factor-beta
    isoform
    dog
    endometrium
    different period
    oestrous cycle
    Opis:
    Both epidermal growth factor (EGF) and transforming growth factor (TGF) play an important physiological role in the processes of proliferation and differentiation of several different cell types. However, the expression profiles of these factors in domestic bitches endometrium are still poorly recognized. The aim of the present study was to identify and analyze the differential expression of these factors in various stages of the estrus cycle. Endometrial tissue from proestrus (n=17), estrus (n=10), day 10 diestrus (n=15), day 35 diestrus (n=18) and anestrus (n=25) was collected soon after ovariohysterectomy. Total RNA was isolated from the endometrium by means of Chomczyński and Sacchi method, treated by DNase I, and reverse- transcribed into cDNA. Quantitative analysis of EGF, TGFβ1, TGFβ2, and TGFβ3 cDNA was performed by real-time quantitative polymerase chain reaction (RT-PCR). EGF expression in canine endometrium was increased in the estrus stage as compared to proestrus (P<0.05), day 10 diestrus (P<0.05), day 35 diestrus (P<0.01) and anestrus (P<0.001). We also found the differences in EGF expression between day 10 and day 35 of estrus as well as between day 35 of estrus with anestrus (P<0.05, P<0.01, respectively). The TGFf1 transcript contents were also higher in estrus as compared to other stages (P<0.01). The TGFβ2 and TGFβ3 in the estrus stage was increased compared to proestrus, day 10 diestrus, day 35 diestrus and anestrus (P<0.05). We proved that expression of EGF and TGFβ transcript isoforms is related to the phase of estrus in bitches and therefore may be regulated by specific hormone concentrations during these periods. Our results confirm the hypothesis that these growth factors play a role in the regulation of biochemical changes in the endometrial tissues during the estrus cycle.
    Źródło:
    Polish Journal of Veterinary Sciences; 2011, 14, 2
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The in vitro culture supplements and selected aspects of canine oocytes maturation
    Autorzy:
    Bukowska, D.
    Kempisty, B.
    Piotrowska, H.
    Zawierucha, P.
    Brussow, K.P.
    Jaskowski, J.M.
    Nowicki, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/32078.pdf
    Data publikacji:
    2012
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    in vitro culture
    supplement
    dog
    oocyte
    maturation
    oestrous cycle
    bitch
    Opis:
    The maturation of oocytes is one of the most important steps determining their developmental competence. Due to the low percentage of oocytes of bitches that reach the MII stage, searching for reagents that may stimulate the growth and maturation of oocytes is still present in this species of mammals. The most important media supplements include gonadotropins (LH, FSH, hCG), growth factors (IGF, TGF, EGF, FGF), progesterone and follicular fluid. It is suggested that the supplement of EGF, and/or follicular cells may have an important influence on the percentage of cells that reach the MII stage. Despite plenty of research based on the improvement of bitch oocytes in vitro culture, the results obtained are still unsatisfactory. Moreover, in the long stages of canine oocytes maturation many molecular and morphological modifications (including changes in mitochondria structure and configuration in the cytoplasm) are involved. In this article, the influence of selected media supplements on the efficiency of bitch oocytes in vitro maturation was described. The molecular and morphological modifications during canine oocytes maturation were also considered in the text.
    Źródło:
    Polish Journal of Veterinary Sciences; 2012, 15, 1
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Nitrogen and Its Fractions in Composts from Wood Waste
    Azot i jego frakcje w kompostach z odpadów drzewnych
    Autorzy:
    Czekała, J.
    Wróblewska, H.
    Piotrowska, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/387837.pdf
    Data publikacji:
    2010
    Wydawca:
    Towarzystwo Chemii i Inżynierii Ekologicznej
    Tematy:
    odpady drzewne
    komposty
    frakcje azotu
    wood waste
    composts
    nitrogen fractions
    Opis:
    Composts produced on the basis of different wood-based industrial waste were analysed for total nitrogen (NT) and its fraction contents. The following nitrogen fractions were separated: water soluble (NWS), hydrolisable (NH) in sulphuric(VI) acid - 2.5 mol dm-3 and at the temperature 105 °C during 12 hours and non-hydrolisable (NNH). Results revealed that tested composts were differentiated by total nitrogen content not only, but also by its content in aqueous and acid extracts. Non-hydrolisable nitrogen represented from 36.22 % to 53.75 % of composts NT. Next, the percentage share of NH2o in NT varied from ca 7.95 % to 11.81 %.
    Komposty wytworzone z różnych odpadów produkcyjnych tworzyw drzewnych, poddano analizie na zawartość azotu ogólnego i jego frakcji. Wydzielono następujące frakcje azotu: wodnorozpuszczalną (NWR), hydrolizującą (NH) w roztworze kwasu siarkowego(VI) - 2,5 M i temperaturze 105 °C przez 12 godzin - i niehydrolizującą (NNH). Stwierdzono, że komposty różniły się nie tylko zawartością całkowitą azotu, ale również jego zawartością w ekstrakcie wodnym oraz kwaśnym. Azot niehydrolizujący stanowił od 36,22 % do 53,75 % azotu ogólnego kompostów. Z kolei udział azotu wodnorozpuszczalnego w zawartości całkowitej wynosił średnio od 7,95 % do 11,81 %.
    Źródło:
    Ecological Chemistry and Engineering. A; 2010, 17, 6; 585-591
    1898-6188
    2084-4530
    Pojawia się w:
    Ecological Chemistry and Engineering. A
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Silver nanoparticle accumulation by aquatic organisms – neutron activation as a tool for the environmental fate of nanoparticles tracing
    Autorzy:
    Asztemborska, M.
    Jakubiak, M.
    Książyk, M.
    Stęborowski, R.
    Polkowska-Motrenko, H.
    Bystrzejewska-Piotrowska, G.
    Powiązania:
    https://bibliotekanauki.pl/articles/146436.pdf
    Data publikacji:
    2014
    Wydawca:
    Instytut Chemii i Techniki Jądrowej
    Tematy:
    silver
    nanoparticles
    neutron activation
    Chironomid larvae
    Danio rerio
    Opis:
    Water environments are noted as being some of the most exposed to the infl uence of toxic nanoparticles (NPs). Therefore, there is a growing need for the investigation of the accumulation and toxicity of NPs to aquatic organisms. In our studies neutron activation followed by gamma spectrometry and liquid scintillation counting were used for studying the accumulation of silver nanoparticles (AgNPs) by freshwater larvae of Chironomus and fi sh Danio rerio. The infl uence of exposition time, concentration and the source of nanoparticles on the effi ciency of AgNP accumulation were studied. It was found that AgNPs are effi ciently accumulated by Chironomid larvae for the fi rst 30 hours of exposition; then, the amount of silver nanoparticles decreases. The silver content in larvae increases together with the NP concentration in water. Larvae which have accumulated AgNPs can be a source of nanoparticles for fi sh and certainly higher levels of Ag in the trophic chain. In comparison with water contamination, silver nanoparticles are more effi ciently accumulated if fi sh are fed with AgNP-contaminated food. Finally, it was concluded that the applied study strategy, including neutron activation of nanoparticles, is very useful technique for tracing the uptake and accumulation of NPs in organisms.
    Źródło:
    Nukleonika; 2014, 59, 4; 169-173
    0029-5922
    1508-5791
    Pojawia się w:
    Nukleonika
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Application of neutron activation for investigation of Fe3O4 nanoparticles accumulation by plants
    Autorzy:
    Bystrzejewska-Piotrowska, G.
    Asztemborska, M
    Stęborowski, R.
    Polkowska-Motrenko, H.
    Danko, B.
    Ryniewicz, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/146840.pdf
    Data publikacji:
    2012
    Wydawca:
    Instytut Chemii i Techniki Jądrowej
    Tematy:
    iron (II,III) oxide
    nanoparticles
    neutron activation
    plants
    Opis:
    As a result of the rapid development of nanotechnology and increasing application of nanoproducts in many areas of everyday life, there is a growing risk of production of nanowastes potentially dangerous for the environment. This makes it necessary to investigate the accumulation and toxicity of nanoparticles (NPs) at different trophic levels. In the studies neutron activation was applied for the investigation of iron (II,III) oxide nanoparticle (Fe3O4-NPs) accumulation by Lepidium sativum and Pisum sativum L. Plants were cultivated on growth medium contaminated with different concentrations (0.01-10 mmolźL-1) of Fe3O4-NPs. For the identification of the presence of Fe3O4-NPs in plant tissues gamma spectrometry following iron oxide (II,III) nanoparticles irradiation was applied. Both plant species were found to accumulate iron (II,III) oxide nanoparticles. The highest content of NPs was found in plant roots, reaching 40 g/kg for Pisum sativum L. More than 90% of accumulated NPs were found in roots. Accumulation of Fe3O4-NPs was found to depend on the concentration of nanostructures in the growth medium. The transfer factor for Lepidium sativum roots and shoots and Pisum sativum L. shoots decreased with increasing NP concentration in the medium; for Pisum sativum L. roots the tendency was reversed. Neutron activation of nanoparticles was shown to be a powerful tool for tracing the environmental fate of NPs and their uptake and accumulation in organisms.
    Źródło:
    Nukleonika; 2012, 57, 3; 427-430
    0029-5922
    1508-5791
    Pojawia się w:
    Nukleonika
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-6 z 6

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