Autor: Pająk, Krzysztof. - Katalog OPAC zbiorów

Skocz do pozycji: 1.

Tytuł:: Native nucleic acid electrophoresis as an efficient alternative for genotyping method of influenza virus
Autorzy:: Pajak, Beata
Lepek, Krzysztof
Powiązania:: https://bibliotekanauki.pl/articles/1039250.pdf
Data publikacji:: 2014
Wydawca:: Polskie Towarzystwo Biochemiczne
Tematy:: gel electrophoresis
SSCP
influenza
minor genetic variants
Opis:: Influenza viruses are the worldwide major causative agents of human and animal acute respiratory infections. Some of the influenza subtypes have caused epidemics and pandemics among humans. The varieties of methods are available for the rapid isolation and identification of influenza viruses in clinical and environmental samples. Since nucleic acids amplification techniques such as RT-PCR have been adapted, fast and sensitive influenza type and subtype determination is possible. However, in some ambiguous cases other, more detailed assay might be desired. The genetic material of influenza virus is highly unstable and constantly mutates. It is known that single nucleotide polymorphisms (SNPs) results in resistance to commercially available anti-viral drugs. The genetic drift of the virus could also result in weakening of immune response to infection. Finally, in a substantial number of patients co-infection with various virus strains or types has been confirmed. Although the detection of co-infection or presence of minor genetic variants within flu-infected patients is not a routine procedure, a rapid and wide spectrum diagnostics of influenza virus infections could reveal an accurate picture of the disease and more importantly, is crucial for choosing the appropriate therapeutics and virus monitoring. Herein we present the evidences that native gel electrophoresis and MSSCP - a method based on multitemperature single strand conformation polymorphism could furnish a useful technique for minor variants, which escape discovery by conventional diagnostic assays.
Źródło:: Acta Biochimica Polonica; 2014, 61, 3; 479-483
0001-527X
Pojawia się w:: Acta Biochimica Polonica
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 2.

Tytuł:: Assessment of pharmacological prophylaxis for acute pancreatitis following ERCP in patients with choledoholithi
Autorzy:: Łubowska-Pająk, Ewa
Kołomecki, Krzysztof
Powiązania:: https://bibliotekanauki.pl/articles/1394242.pdf
Data publikacji:: 2015
Wydawca:: Index Copernicus International
Tematy:: acute pancreatitis
endoscopic retrograde cholangiopancreatography
Opis:: Endoscopic retrograde cholangiopancreatography (ERCP) is an effective tool in the diagnostics and treatment of bile duct diseases. Although minimally invasive, the procedure is associated with a risk of complications, with acute pancreatitis being the most serious. In recent years, high hopes have been placed on pharmacological prevention of acute pancreatitis after ERCP. The aim of the study was assessment of the efficacy of low-molecular-weight heparin and somatostatin in combination with diclofenac in the prevention of acute pancreatitis after ERCP. Material and methods. The study enrolled three groups of 30 patients diagnosed with cholelithiasis; group I: patients who received low-molecular-weight heparin prior to ERCP, group II: patients who received somatostatin and diclofenac, group III: control group. The study assessed the incidence of acute pancreatitis, hyperamylasemia and increased CRP levels. Results. Acute pancreatitis was observed in 13.3% of group I patients, 10% of group II patients and 16.7% of group III patients (no statistical significance). Hyperamylasemia was observed in 16.7% of group I patients, 16.7% of group II patients and 43.3% of group III patients. These differences were statistically significant. No significant differences were found in the occurrence of increased CRP levels among the study groups. Conclusions. No significant reduction in the occurrence of acute pancreatitis after ERCP was observed in patients who received pharmacological prophylaxis. A significant reduction in the occurrence of hyperamylasemia was found in drug-treated patients
Źródło:: Polish Journal of Surgery; 2015, 87, 12; 620-625
0032-373X
2299-2847
Pojawia się w:: Polish Journal of Surgery
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 3.

Tytuł:: Anomalous origin of the right coronary artery from the main pulmonary artery treated surgically in a 6-week-old infant. A case report and review of the literature
Nieprawidłowe ujście prawej tętnicy wieńcowej od pnia tętnicy płucnej leczone chirurgicznie u 6-tygodniowego niemowlęcia. Opis przypadku i przegląd piśmiennictwa
Autorzy:: Grabowski, Krzysztof
Karolczak, Maciej Aleksander
Zacharska-Kokot, Ewa
Mądry, Wojciech
Pająk, Jacek
Powiązania:: https://bibliotekanauki.pl/articles/1033284.pdf
Data publikacji:: 2018
Wydawca:: Medical Communications
Tematy:: echocardiographic diagnosis
Opis:: Background: In the present paper, the authors describe a case of a rare congenital defect – anomalous origin of the right coronary artery from the main pulmonary artery diagnosed in a 5-week-old infant who was deemed eligible for surgical treatment based solely on echocardiography. Such anatomical abnormalities of the coronary arteries are subtle and thus extremely difficult to visualize, especially in patients in whom permanent extensive damage of the cardiac muscle has not yet occurred. For this reason, the diagnosis is usually established when the disease is highly advanced, oftentimes only postmortem. In this paper, the authors present early echocardiographic evaluation, successful surgical treatment and post-operative echocardiographic examination. Case presentation: Fetal echocardiographic examinations demonstrated single vascular anomalies. The suspicion was verified after birth. Other congenital defects, including genetic defects, were also investigated. In the 5th week of life, cardiac ECHO showed abnormal origin of the right coronary artery from the trunk of the pulmonary artery. The child was deemed eligible for surgical treatment of the defect. The surgical translocation of the ostium of the right coronary artery to the aorta was done with success, and the child was discharged on the 14th day of hospitalization. Conclusion: The present case report demonstrates that careful examination of the child with precise echocardiographic examination makes it possible to establish an early diagnosis of this rare defect. This discrete defect can be treated early, before the symptoms, often connected with irreversible changes in the myocardium, appear.
W prezentowanej pracy autorzy opisują przypadek rzadkiej wady wrodzonej – nieprawidłowego ujścia prawej tętnicy wieńcowej od pnia tętnicy płucnej, rozpoznanego u 5-tygodniowego niemowlęcia, które zostało zakwalifikowane do leczenia kardiochirurgicznego wyłącznie na podstawie badania echokardiograficznego. Takie anatomiczne nieprawidłowości tętnic wieńcowych są subtelne, a przez to niezwykle trudne do wizualizacji, szczególnie u pacjentów, u których nie doszło jeszcze do trwałego, rozległego uszkodzenia mięśnia serca. Z tego względu diagnozę zazwyczaj stawia się, gdy choroba jest znacznie zaawansowana, nierzadko dopiero po śmierci. W niniejszej pracy autorzy opisują wczesne rozpoznanie na podstawie diagnostyki echokardiograficzej, skuteczne chirurgiczne leczenie i pooperacyjne badanie echokardiograficzne. Opis przypadku: Na podstawie badań echokardiograficznych u ciężarnej wysunięto podejrzenie nieprawidłowości układu naczyniowego u płodu. Po urodzeniu podejrzenie zostało potwierdzone. U noworodka rozpoznano również inne wrodzone wady i poszukiwano cech zespołu genetycznego. W 5. tygodniu życia badanie echokardiograficzne wykazało nieprawidłowe ujście prawej tętnicy wieńcowej od pnia tętnicy płucnej. Dziecko zakwalifikowano do leczenia chirurgicznego wady. Chirurgiczne przemieszczenie ujścia prawej tętnicy wieńcowej do aorty zostało wykonane z powodzeniem i dziecko wypisano w 14. dniu hospitalizacji. Wnioski: Opisany przypadek pokazuje, że dokładne badanie dziecka wraz z precyzyjnym badaniem echokardiograficznym pozwala ustalić wczesne rozpoznanie w rzadkich chorobach. Dyskretnie objawiająca się wada może być leczona wcześnie, zanim ujawnią się objawy, zazwyczaj związane z nieodwracalnymi zmianami w myocardium. Artykuł w wersji polskojęzycznej jest dostępny na stronie http://jultrason.pl/index.php/issues/volume-18-no-72
Źródło:: Journal of Ultrasonography; 2018, 18, 72; 71-76
2451-070X
Pojawia się w:: Journal of Ultrasonography
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 4.

Tytuł:: miRNA-146a-5p is upregulated in serum and cartilage samples of patients with osteoarthritis
Autorzy:: Skrzypa, Marzena
Szala, Dariusz
Gablo, Natalia
Czech, Joanna
Pajak, Justyna
Kopanska, Marta
Trzeciak, Mateusz
Gargasz, Krzysztof
Snela, Sławomir
Zawlik, Izabela
Powiązania:: https://bibliotekanauki.pl/articles/1391948.pdf
Data publikacji:: 2019
Wydawca:: Index Copernicus International
Tematy:: circulating microRNA
osteoarthritis
serum
cartilage
biomarker
Opis:: Introduction: Osteoarthritis (OA) is a widely prevalent joint disease leading to motor disability and pain. Appropriate indicators for identifying patients at risk for this progressive disease, identifying molecular events for detecting early phases of the disease, or biomarkers to screen for treatment responses, however, are lacking. Micro RNAs (miRNAs), which play crucial roles in OA, could be potential biomarkers of OA. Because circulating miRNA levels reflect the disease state, they may be useful for OA screening and as diagnostic tools, reducing the need for invasive procedures and minimizing the cost of current diagnostic methods. Materials and methods: The expression levels of 18 microRNAs (let-7e-5p, miR-21-5p, miR-93-5p, miR-101-3p, miR-103a-3p, miR-130a-3p miR-146a-5p, miR-16-5p, miR-193b-3p miR-199a-3p, miR-210-3p, miR-222-3p, miR-22-3p, miR-27a-3p, miR-27b-3p, miR-335-5p, miR-454-3p, and miR-98-5p) were analyzed by quantitative real-time polymerase chain reaction in the cartilage tissues and serum samples of 28 OA patients and were compared to those of 2 healthy controls. Results: Expression of microRNA-146a-5p was significantly upregulated in the cartilage (p=0.006) and serum (p=0.002) of OA patients. The expression levels of miR-146a-5p in the serum were positively correlated with those in the cartilage (Pearson correlation coefficient R=0.32; p=0.002). Conclusion: miR-146a-5p was significantly overexpressed in patients with OA, both in the articular cartilage tissue and serum, with a positive correlation between the levels in both types of samples. Therefore, the miR-146a-5p serum level could reflect the molecular processes in the cartilage, suggesting its clinical utility as a biomarker for OA management. Implementing noninvasive biomarker using serum miRNAs involves the analysis of the misregulated miRNAs linked to the cartilage pathology.
Źródło:: Polish Journal of Surgery; 2019, 91, 3; 1-5
0032-373X
2299-2847
Pojawia się w:: Polish Journal of Surgery
Dostawca treści:: Biblioteka Nauki

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