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Wyszukujesz frazę "Michalak, Krystyna" wg kryterium: Autor


Wyświetlanie 1-4 z 4
Tytuł:
Evaluation of Resistance to Potato Virus Y of Cultivars in Pre-registration (Statutory) Trials
Autorzy:
Michalak, Krystyna
Chrzanowska, Mirosława
Powiązania:
https://bibliotekanauki.pl/articles/2199683.pdf
Data publikacji:
2017-06-20
Wydawca:
Instytut Hodowli i Aklimatyzacji Roślin
Tematy:
field test
growing-on test
greenhouse test
potato cultivar
PVY
Opis:
The paper describes testing potato cultivars for resistance to potato viruses in field and greenhouse condi-tions according to the scheme of the statutory trials. Seed tubers of tested cultivar are planted in the field in two replications with 30 tubers each. On both sides of each cultivar, 15 PVY-infected tubers, i.e. the source for PVY inoculation, are planted. The evaluation includes standard cultivars of known resistance levels to PVY. The progeny tubers are collected in autumn and growing-on testing is carried out in greenhouse condi-tions to estimate the number of plants infected with PVY. The other method is to evaluate the resistance of potato cultivars in greenhouse conditions. At present, the resistance of cultivars is evaluated only with respect to PVY, the most important potato virus. Two strains are used in the evaluation, i.e. PVYN-Wi and PVYNTN. For each testing cultivar, 10 plants are mechanically inoculated with each PVY strain. The analysis of the results makes it possible to classify the cultivars into four resistance groups on a 1-9 scale: highly resistant (with the resistance score of 8), resistant (with a value of 7), mid-resistant (with a value 5-6), and susceptible (with a value of 3-4). The ”9” rating is specified after the registration of the cultivars based on molecular tests, that validate the presence of a marker linked to extreme resistance (ER) gene Rysto.
Źródło:
Plant Breeding and Seed Science; 2017, 75; 63-70
1429-3862
2083-599X
Pojawia się w:
Plant Breeding and Seed Science
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Collection of Potato Viral Pathogens, Its Validation and Maintenance
Autorzy:
Yin, Zhimin
Michalak, Krystyna
Lebecka, Renata
Powiązania:
https://bibliotekanauki.pl/articles/2199676.pdf
Data publikacji:
2017-06-20
Wydawca:
Instytut Hodowli i Aklimatyzacji Roślin
Tematy:
potato viruses
in vitro plantlets
freeze-dried leaves
frozen leaves
Opis:
IHAR – PIB collection of potato viral pathogens has been maintained since 1970s. Currently there are 258 isolates in the collection representing 12 potato viruses, named PVY, PVM, PVS, PLRV, PVA, PVX, PAMV, BMYV, TBRV, AMV, CMV and TRV. The viruses are maintained in potato plants in vivo in the greenhouse (246 isolates), in potato plantlets cultured in vitro (12 isolates), in frozen leaves (two copies each of the 246 isolates) and in freeze-dried tissue (234 isolates).
Źródło:
Plant Breeding and Seed Science; 2017, 75; 57-62
1429-3862
2083-599X
Pojawia się w:
Plant Breeding and Seed Science
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Giant unilamellar vesicles - a perfect tool to visualize phase separation and lipid rafts in model systems
Autorzy:
Wesołowska, Olga
Michalak, Krystyna
Maniewska, Jadwiga
Hendrich, Andrzej
Powiązania:
https://bibliotekanauki.pl/articles/1040608.pdf
Data publikacji:
2009
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
phase separation
lipid rafts
model membranes
giant unilamellar vesicles
Opis:
Model systems such as black lipid membranes or conventional uni- or multilamellar liposomes are commonly used to study membrane properties and structure. However, the construction and dimensions of these models excluded their direct optical microscopic observation. Since the introduction of the simple method of liposome electroformation in alternating electric field giant unilamellar vesicles (GUVs) have become an important model imitating biological membranes. Due to the average diameter of GUVs reaching up to 100 µm, they can be easily observed under a fluorescent or confocal microscope provided that the appropriate fluorescent probe was incorporated into the lipid phase during vesicle formation. GUVs can be formed from different lipid mixtures and they are stable in a wide range of physical conditions such as pH, pressure or temperature. This mini-review presents information about the methods of GUV production and their usage. Particularly, the use of GUVs in studying lipid phase separation and the appearance and behavior of lipid domains (rafts) in membranes is discussed but also other examples of GUVs use in membrane research are given. The experience of the authors in setting up the GUV-forming equipment and production of GUVs is also presented.
Źródło:
Acta Biochimica Polonica; 2009, 56, 1; 33-39
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The interaction of new oxicam derivatives with lipid bilayers as measured by calorimetry and fluorescence spectroscopy
Autorzy:
Maniewska, Jadwiga
Gąsiorowska, Justyna
Szczęśniak-Sięga, Berenika
Michalak, Krystyna
Powiązania:
https://bibliotekanauki.pl/articles/1038386.pdf
Data publikacji:
2018
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
oxicam derivatives
NSAIDs analogues
piroxicam
1,2- benzothiazine derivatives synthesis
model lipid bilayers
differential scanning calorimetry (DSC)
fluorescence spectroscopy
Opis:
The purpose of the present work was to assess the ability of five new oxicam analogues to interact with the lipid bilayers. To characterize the interaction of newly synthesized NSAIDs (non-steroidal anti-inflammatory drugs) analogues with DPPC lipid bilayers the two following techniques were applied - differential scanning calorimetry (DSC) and fluorescence spectroscopy. The results obtained by these experimental approaches show that new oxicams analogues interact with the lipid model membranes under consideration. As demonstrated both in calorimetric and spectroscopic studies, the greatest influence on the thermotropic properties of the lipid membrane and on the quenching of fluorescence of Laurdan and Prodan was exerted by a derivative named PR47 containing in its structure a two-carbon aliphatic linker with a carbonyl group, as well as bromine and trifluoromethyl substituents.
Źródło:
Acta Biochimica Polonica; 2018, 65, 2; 185-191
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
    Wyświetlanie 1-4 z 4

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