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    Wyświetlanie 1-5 z 5
    Tytuł:
    Kinetic intermediates of unfolding of dimeric prostatic phosphatase
    Autorzy:
    Kuciel, Radosława
    Mazurkiewicz, Aleksandra
    Dudzik, Paulina
    Powiązania:
    https://bibliotekanauki.pl/articles/1041089.pdf
    Data publikacji:
    2007
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    dimer
    prostatic phosphatase
    protein unfolding
    Opis:
    Kinetics of guanidine hydrochloride (GdnHCl)-induced unfolding of human prostatic acid phosphatase (hPAP), a homodimer of 50 kDa subunit molecular mass was investigated with enzyme activity measurements, capacity for binding an external hydrophobic probe, 1-anilinonaphtalene-8-sulfonate (ANS), accessibility of thiols to reaction with 5,5'-dithiobis(2-nitrobenzoic acid) (DTNB) and 2-(4'-maleimidylanilino)naphthalene-6-sulfonate (MIANS) and ability to bind Congo red dye. Kinetic analysis was performed to describe a possible mechanism of hPAP unfolding and dissociation that leads to generation of an inactive monomeric intermediate that resembles, in solution of 1.25 M GdnHCl pH 7.5, at 20°C, in equilibrium, a molten globule state. The reaction of hPAP inactivation in 1.25 M GdnHCl followed first order kinetics with the reaction rate constant 0.0715 ± 0.0024 min-1 . The rate constants of similar range were found for the pseudo-first-order reactions of ANS and Congo red binding: 0.0366 ± 0.0018 min-1 and 0.0409 ± 0.0052 min-1, respectively. Free thiol groups, inaccessible in the native protein, were gradually becoming, with the progress of unfolding, exposed for the reactions with DTNB and MIANS, with the pseudo-first-order reaction rate constants 0.327 ± 0.014 min-1 and 0.216 ± 0.010 min-1, respectively. The data indicated that in the course of hPAP denaturation exposure of thiol groups to reagents took place faster than the enzyme inactivation and exposure of the protein hydrophobic surface. This suggested the existence of a catalytically active, partially unfolded, but probably dimeric kinetic intermediate in the process of hPAP unfolding. On the other hand, the protein inactivation was accompanied by exposure of a hydrophobic, ANS-binding surface, and with an increased capacity to bind Congo red. Together with previous studies these results suggest that the stability of the catalytically active conformation of the enzyme depends mainly on the dimeric structure of the native hPAP.
    Źródło:
    Acta Biochimica Polonica; 2007, 54, 2; 371-377
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Immunoreactivity of proteolytic degradation products of human prostatic acid phosphatase
    Autorzy:
    Kuciel, Radosława
    Mazurkiewicz, Aleksandra
    Steuden, Iga
    Wasylewska, Ewa
    Szkudlarek, Jerzy
    Dulińska, Joanna
    Ostrowski, Włodzimierz
    Powiązania:
    https://bibliotekanauki.pl/articles/1045865.pdf
    Data publikacji:
    1988
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Źródło:
    Acta Biochimica Polonica; 1988, 35, 4; 319-330
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Microscopic fungi on Schoenoplectus lacustris in Plociczno and Plociowe lakes in Drawa National Park (northwest Poland)
    Autorzy:
    Mazurkiewicz-Zapałowicz, Kinga
    Rybińska, Aleksandra
    Łopusiewicz, Łukasz
    Powiązania:
    https://bibliotekanauki.pl/articles/952562.pdf
    Data publikacji:
    2017
    Wydawca:
    Polskie Towarzystwo Botaniczne
    Tematy:
    biodiversity
    microfungi
    schoenoplectus
    drawa national park
    Opis:
    In the period 2009–2011, the micromycetes causing symptoms on the leaves of Schoenoplectus lacustris (SL) from Płociczno and Płociowe lakes in Drawa National Park (DNP) were investigated. A total of 39 taxa of microscopic fungi and chromistan fungi were found. The taxonomic structure of the mycobiota was dominated by anamorphs of Ascomycota. In each year of the study, the SL fungal species richness was higher in Płociowe Lake than in Płociczno Lake. In all the years of study in both lakes, the fungal community on SL was composed of the following six dominant species: Alternaria alternata, Cladosporium herbarum, Fusarium incarnatum, F. sacchari, Gibberella avenacea, and Pleospora scirpicola. The species occurring exclusively in Płociczno Lake were Papulaspora immersa, Puccinia scirpi, and Trichothecium roseum, and those found only in Płociowe Lake were Acremoniella atra, Alternaria atra, Aspergillus niger, A. versicolor, Fusarium oxysporum, Gonatobotrys simplex, Massariosphaeria scirpina, Microascus brevicaulis, Penicillium chrysogenum, and Stagonospora aquatica. Only one confirmation of the occurrence of Puccinia scirpi and the lack of other specialized obligate parasites indicate the good health of SL in DNP. The facultative parasites, inter alia, Boeremia exigua, Chaetomium globosum, Fusarium culmorum, F. incarnatum, F. sacchari, Gibberella avenacea, and Stagonospora aquatica that are present on plants as part of the natural process of apoptosis may influence the health of SL.
    Źródło:
    Acta Mycologica; 2017, 52, 2
    0001-625X
    2353-074X
    Pojawia się w:
    Acta Mycologica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    A Possibility of Functioning Biogas Plant at a Poultry Farm
    Autorzy:
    Janczak, Damian
    Mazurkiewicz, Jakub
    Czekała, Wojciech
    Myszura, Magdalena
    Kozłowski, Kamil
    Jeżowska, Aleksandra
    Powiązania:
    https://bibliotekanauki.pl/articles/125450.pdf
    Data publikacji:
    2019
    Wydawca:
    Polskie Towarzystwo Inżynierii Ekologicznej
    Tematy:
    poultry waste
    biogas
    renewable energy
    environmental protection
    sustainable development
    animal production
    Opis:
    The biogas production constitutes one of renewable energy sources (RES) . In addition, wastes are preferred for energy production. In the case of some wastes, e.g. poultry manure, it is difficult to conduct anaerobic digestion in monofermentation. The aim of this work was to plan the biogas plant, in which the main substrate is the waste from a poultry farm. The scope of work included: preparation of a biogas plant technological project, determining the amount of biogas and methane that can be produced annually on the example of the selected poultry farm, performing the energy and financial calculations for the current conditions prevailing on the renewable energy market in Poland. The installation project assumed the location of a biogas plant at an existing poultry farm – the source of the substrate. The micro-biogas plant includes a fermentation tank with a capacity of 500 m3 and storage of digestate pulp with a capacity of 700 m3. The assumed power biogas plant will generate 112 kW of electricity and 120 kW of heat. The installation will operate in a single-stage mesophilic technology (39 °C), which will avoid incurring additional costs related to heating and the construction of additional fermentation tanks. The use of poultry manure by anaerobic digestion provides benefits through biogas technology. It is necessary to examine the technology in terms of biogas production, which is carried out under better sanitary and environmental conditions. This work was undertaken to investigate the environmentally friendly removal of poultry manure through biogas technology to obtain the best economic effect, and employ it further, e.g. as a fertilizer.
    Źródło:
    Journal of Ecological Engineering; 2019, 20, 11; 225-231
    2299-8993
    Pojawia się w:
    Journal of Ecological Engineering
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-5 z 5

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