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    Wyświetlanie 1-14 z 14
    Tytuł:
    Characterization of disulfide exchange between DsbA and HtrA proteins from Escherichia coli
    Autorzy:
    Skórko-Glonek, Joanna
    Sobiecka-Szkatuła, Anna
    Lipińska, Barbara
    Powiązania:
    https://bibliotekanauki.pl/articles/1041221.pdf
    Data publikacji:
    2006
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    DsbA oxidoreductase
    disulfide exchange
    HtrA protease
    stoichiometry of HtrA-DsbA interaction
    kinetics of HtrA oxidation
    Opis:
    DsbA is the major oxidase responsible for generation of disulfide bonds in proteins of E. coli envelope. In the present work we provided the first detailed characterization of disulfide exchange between DsbA and its natural substrate, HtrA protease. We demonstrated that HtrA oxidation relies on DsbA, both in vivo and in vitro. We followed the disulfide exchange between these proteins spectrofluorimetrically and found that DsbA oxidizes HtrA with a 1 : 1 stoichiometry. The calculated second-order apparent rate constant (kapp) of this reaction was 3.3 × 104 ± 0.6 × 104 M-1s-1. This value was significantly higher than the values obtained for nonfunctional disulfide exchanges between DsbA and DsbC or DsbD and it was comparable to the kapp values calculated for in vitro oxidation of certain non-natural DsbA substrates of eukaryotic origin.
    Źródło:
    Acta Biochimica Polonica; 2006, 53, 3; 585-589
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Epidemiology of silicosis reported to the central register of occupational diseases over last 20 years in Poland
    Autorzy:
    Nowak-Pasternak, Joanna
    Lipińska-Ojrzanowska, Agnieszka
    Świątkowska, Beata
    Powiązania:
    https://bibliotekanauki.pl/articles/2152952.pdf
    Data publikacji:
    2022
    Wydawca:
    Instytut Medycyny Pracy im. prof. dra Jerzego Nofera w Łodzi
    Tematy:
    Polska
    occupational diseases
    epidemiology
    silica
    silicosis
    pneumoconioses
    Opis:
    Objectives The aim of the study was to investigate and assess the incidence of silicosis cases acknowledged as occupational diseases in Poland in 2000–2019. Material and Methods The cases of all medically recognized pneumoconioses, including silicoses, certified as occupational diseases were studied. The records were extracted from the Central Register of Occupational Diseases, the only official Polish central electronic data base of occupational diseases. Results During the period 2000–2019, 2066 confirmed cases of silicoses and 10 665 cases of other pneumoconioses including asbestosis and coal workers’ pneumoconiosis were reported to the Central Register of Occupational Diseases. Silicoses accounted for 12.8–21.2% of all pneumoconioses. The number of confirmed silicoses cases was growing along with the length of latency period and was the highest for the period of ≥40 years (513 cases). Over 70% of silicoses cases occurred after occupational exposure >20 years. The most workers who evolved silicosis were employed in manufacturing, predominantly casting of iron, mining and quarrying and construction. Conclusions The number of confirmed cases of silicosis in Poland decreased in 2000–2019 but the disease still remains an important health problem. Prevention is crucial to reduce further disease incidence. The medical monitoring standards of exposed workers should be improved. Developing new diagnosing guidelines with the use of other imaging examinations, like high-resolution computed tomography, has to be considered. The analysis should contribute into the implementation of silicosis preventative programmes, both at the enterprise and national level.
    Źródło:
    International Journal of Occupational Medicine and Environmental Health; 2022, 35, 5; 561-570
    1232-1087
    1896-494X
    Pojawia się w:
    International Journal of Occupational Medicine and Environmental Health
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Concept of health surveillance programme for workers exposed to respirable crystalline silica at present and in the past
    Autorzy:
    Nowak-Pasternak, Joanna
    Świątkowska, Beata
    Lipińska-Ojrzanowska, Agnieszka
    Powiązania:
    https://bibliotekanauki.pl/articles/23388842.pdf
    Data publikacji:
    2023-11-14
    Wydawca:
    Instytut Medycyny Pracy im. prof. dra Jerzego Nofera w Łodzi
    Tematy:
    epidemiology
    silicosis
    lung diseases
    occupational diseases
    prevention and control
    silicon dioxide
    Opis:
    In the paper authors present general assumptions of health surveillance programme concept for workers employed in respirable crystalline silica (RCS) exposure at present and in the past. There is no effective treatment for silicosis thus disease prevention is of paramount significance. For decades efforts of World Health Organization (WHO) and International Work Organization (ILO) have been focused on eliminating silicosis globally. Unfortunately silicosis is still one of the most lethal occupational diseases and the preventative programmes have not yet been successful. The authors identify main steps to complete an overview of RCS exposure and suggest lines of actions to be taken before launching the health surveillance programme. Introduction of the health surveillance programme would increase awareness of harmful health effects of the RCS exposure, emphasize the significance of preventive medical check-ups and early diagnostics of occupational diseases as well as the importance of using appropriate protective equipment. The programme development on a national level might be carried out with the cooperation of multiple backgrounds and institutions. This would allow for detailed planning, implementation, monitoring and effective evaluation of its results. Having a better and updated knowledge of silicosis epidemiology, early diagnostics, the possible sources of RCS occupational exposure and evaluation of undertaken preventive actions are crucial factors in disease prevention. The programme introduction would be of educational significance for all the stakeholders and the groups engaged in the project implementation, which would contribute to high effectiveness of the preventive activities and their improvement in the future.
    Źródło:
    Medycyna Pracy. Workers’ Health and Safety; 2023, 74, 4; 341-346
    0465-5893
    2353-1339
    Pojawia się w:
    Medycyna Pracy. Workers’ Health and Safety
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Cloning of the groE operon of the marine bacterium Vibrio harveyi using a lambda vector
    Autorzy:
    Kuchanny, Dorota
    Klein, Gracjana
    Krzewska, Joanna
    Czyż, Agata
    Lipińska, Barbara
    Powiązania:
    https://bibliotekanauki.pl/articles/1044880.pdf
    Data publikacji:
    1998
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Źródło:
    Acta Biochimica Polonica; 1998, 45, 1; 261-270
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Escherichia coli small heat shock proteins IbpA/B enhance activity of enzymes sequestered in inclusion bodies.
    Autorzy:
    Kuczyńska-Wiśnik, Dorota
    Żurawa-Janicka, Dorota
    Narkiewicz, Joanna
    Kwiatkowska, Joanna
    Lipińska, Barbara
    Laskowska, Ewa
    Powiązania:
    https://bibliotekanauki.pl/articles/1041504.pdf
    Data publikacji:
    2004
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    protein aggregation
    inclusion bodies
    IbpA/B
    Opis:
    Escherichia coli small heat shock proteins, IbpA/B, function as molecular chaperones and protect misfolded proteins against irreversible aggregation. IbpA/B are induced during overproduction of recombinant proteins and bind to inclusion bodies in E. coli cells. We investigated the effect of ΔibpA/B mutation on formation of inclusion bodies and biological activity of enzymes sequestered in the aggregates in E. coli cells. Using three different recombinant proteins: Cro-β-galactosidase, β-lactamase and rat rHtrA1 we demonstrated that deletion of the ibpA/B operon did not affect the level of produced inclusion bodies. However, in aggregates containing IbpA/B a higher enzymatic activity was detected than in the IbpA/B-deficient inclusion bodies. These results confirm that IbpA/B protect misfolded proteins from inactivation in vivo.
    Źródło:
    Acta Biochimica Polonica; 2004, 51, 4; 925-931
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Znaczenie kliniczne ekspresji neuropiliny-1 u chorych na raka jajnika
    Clinical signifi cance of neuropilin-1 expression in patients with ovarian cancer
    Autorzy:
    Klasa-Mazurkiewicz, Dagmara
    Narkiewicz, Joanna
    Milczek, Tomasz
    Lipińska, Barbara
    Emerich, Janusz
    Wydra, Dariusz
    Powiązania:
    https://bibliotekanauki.pl/articles/1030638.pdf
    Data publikacji:
    2009
    Wydawca:
    Medical Communications
    Tematy:
    angiogenesis
    lymphangiogenesis
    neuropilin
    ovarian cancer
    prognostic factors
    angiogeneza
    czynniki prognostyczne
    kluczowe: rak jajnika
    limfangiogeneza
    neuropilina
    Opis:
    Background: Angiogenesis is a key process in the development of a malignant tumor, enabling both growth of primary lesion and spread of metastases. Most potent stimulators of normal and pathological angiogenesis are proteins of the vascular endothelial growth factor (VEGF) family. Regulation of angiogenesis process is also mediated by neuropilin- 1 (NP-1), as coreceptor VEGFR-2. NP-1 plays a crucial role controlling both normal angiogenesis during embryonal development and pathological angiogenesis in malignant tumors. The aim of this paper was to assess NP-1 expression in ovarian cancer and to analyze correlations between NP-1 expression and selected clinical-pathological factors in a group of ovarian cancer patients. Material and method: Analyzed was the relative level of NP-1 in 168 surgical specimens collected during surgical procedures performed at the Department of Oncologic Gynecology of Medical University in Gdańsk. Tissue samples included: 32 healthy tissues, 42 benign tumors, 10 borderline tumors, 76 ovarian cancers, 8 metastatic tumors. Relative NP-1 level was assessed using Western blotting technology. Results: Overexpression of NP-1 was seen significantly more often in early clinical stages of ovarian cancer (p=0.01), in cancers other than serous (p=0.04) and in patients with peritoneal exudate (p=0.03). Log-rank test did not reveal any significant correlation between NP-1 expression and favorable response to chemotherapy, disease-free survival or total survival time. Conclusions. Elevated NP-1 level seen in initial clinical stages of ovarian cancer may indicate crucial role of neoangiogenesis in the early phase of tumor development.
    Źródło:
    Current Gynecologic Oncology; 2009, 7, 4; 237-247
    2451-0750
    Pojawia się w:
    Current Gynecologic Oncology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Graphene oxide modified with collagen i for myocardial tissue regeneration
    Autorzy:
    Jagiello, Joanna
    Gwiazda, Marcin
    Kijenska-Gawronska, Ewa
    Baran, Magdalena
    Lipinska, Ludwika
    Swieszkowski, Wojciech
    Powiązania:
    https://bibliotekanauki.pl/articles/284824.pdf
    Data publikacji:
    2019
    Wydawca:
    Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
    Źródło:
    Engineering of Biomaterials; 2019, 22, no.153; 30
    1429-7248
    Pojawia się w:
    Engineering of Biomaterials
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Can medical staff have visible tattoos? A survey study among students
    Autorzy:
    Paprocka-Lipińska, Anna
    Majeranowski, Alan
    Drozd-Garbacewicz, Małgorzata
    Mazanek, Małgorzata
    Hebdzińska, Maria
    Styszko, Joanna
    Powiązania:
    https://bibliotekanauki.pl/articles/895813.pdf
    Data publikacji:
    2020-01-09
    Wydawca:
    Gdański Uniwersytet Medyczny
    Tematy:
    health care professionals
    visible tattoos
    dress code
    Opis:
    Background: Tattoo is becoming a more and more common form of body decoration of people from representatives of various professions.The aim of this study was: what is the opinion of university students about medical staff having visible tattoos. Material and methods: The study was conducted among the students of four universities in Gdańsk. The questionnaire contained questions about the acceptance of visible tattoos in people working in medical professions. The respondents were also asked about their attitudes to tattoos as a form of body decoration and whether they had tattoos themselves. The statistical analysis was performed using IBM SPSS Statistics 25 software. Results: Filled-in questionnaires were obtained from 676 respondents, including 477 students of the medical university and 199 students of non-medical universities. The students who have tattoos themselves accept visible tattoos in healthcare professionals to a significantly greater extent (92%) than those who do not have tattoos, but the acceptance of visible tattoos in healthcare professionals in this group turned out to be very high too (75%). The respondents most readily accepted a visible tattoo on the skin of a laboratory diagnostician (75%). Conclusions: University students, regardless of their university profile, consider a visible tattoo as a completely acceptable phenomenon in healthcare professionals.
    Źródło:
    European Journal of Translational and Clinical Medicine; 2019, 2, 2; 23-26
    2657-3148
    2657-3156
    Pojawia się w:
    European Journal of Translational and Clinical Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Utilization of graphenebased materials and mesenchymal stem cells in regenerative medicine
    Autorzy:
    Sekuła-Stryjewska, Małgorzata
    Noga, Sylwia
    Byrska, Aleksandra
    Pabis, Marta
    Lelek, Joanna
    Jagiełło, Joanna
    Madeja, Zbigniew
    Lipińska, Ludwika
    Zuba-Surma, Ewa
    Powiązania:
    https://bibliotekanauki.pl/articles/284357.pdf
    Data publikacji:
    2019
    Wydawca:
    Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
    Źródło:
    Engineering of Biomaterials; 2019, 22, no.153; 55
    1429-7248
    Pojawia się w:
    Engineering of Biomaterials
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The DnaK chaperones from the archaeon Methanosarcina mazei and the bacterium Escherichia coli have different substrate specificities
    Autorzy:
    Żmijewski, Michal
    Skórko-Glonek, Joanna
    Tanfani, Fabio
    Banecki, Bogdan
    Kotlarz, Agnieszka
    Macario, Alberto
    Lipińska, Barbara
    Powiązania:
    https://bibliotekanauki.pl/articles/1040934.pdf
    Data publikacji:
    2007
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    archaeal DnaK quaternary structure
    archaeal Hsp70(DnaK)
    substrate-binding by archaeal DnaK
    Opis:
    Hsp70 (DnaK) is a highly conserved molecular chaperone present in bacteria, eukaryotes, and some archaea. In a previous work we demonstrated that DnaK from the archaeon Methanosarcina mazei (DnaKMm) and the DnaK from the bacterium Escherichia coli (DnaKEc) were functionally similar when assayed in vitro but DnaKMm failed to substitute for DnaKEc in vivo. Searching for the molecular basis of the observed DnaK species specificity we compared substrate binding by DnaKMm and DnaKEc. DnaKMm showed a lower affinity for the model peptide (a-CALLQSRLLS) compared to DnaKEc. Furthermore, it was unable to negatively regulate the E. coli σ32 transcription factor level under heat shock conditions and poorly bound purified σ32, which is a native substrate of DnaKEc. These observations taken together indicate differences in substrate specificity of archaeal and bacterial DnaKs. Structural modeling of DnaKMm showed some structural differences in the substrate-binding domains of DnaKMm and DnaKEc, which may be responsible, at least partially, for the differences in peptide binding. Size-exclusion chromatography and native gel electrophoresis revealed that DnaKMm was found preferably in high molecular mass oligomeric forms, contrary to DnaKEc. Oligomers of DnaKMm could be dissociated in the presence of ATP and a substrate (peptide) but not ADP, which may suggest that monomer is the active form of DnaKMm.
    Źródło:
    Acta Biochimica Polonica; 2007, 54, 3; 509-522
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Structural basis of the interspecies interaction between the chaperone DnaK(Hsp70) and the co-chaperone GrpE of archaea and bacteria
    Autorzy:
    Żmijewski, Michał
    Skórko-Glonek, Joanna
    Tanfani, Fabio
    Banecki, Bogdan
    Kotlarz, Agnieszka
    Macario, Alberto
    Lipińska, Barbara
    Powiązania:
    https://bibliotekanauki.pl/articles/1041069.pdf
    Data publikacji:
    2007
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    substrate-binding domain
    DnaK-GrpE complex
    archaeal Hsp70(DnaK)
    archaeal DnaK structure
    molecular chaperones
    ATPase domain
    Opis:
    Hsp70s are chaperone proteins that are conserved in evolution and present in all prokaryotic and eukaryotic organisms. In the archaea, which form a distinct kingdom, the Hsp70 chaperones have been found in some species only, including Methanosarcina mazei. Both the bacterial and archaeal Hsp70(DnaK) chaperones cooperate with a GrpE co-chaperone which stimulates the ATPase activity of the DnaK protein. It is currently believed that the archaeal Hsp70 system was obtained by the lateral transfer of chaperone genes from bacteria. Our previous finding that the DnaK and GrpE proteins of M. mazei can functionally cooperate with the Escherichia coli GrpE and DnaK supported this hypothesis. However, the cooperation was surprising, considering the very low identity of the GrpE proteins (26%) and the relatively low identity of the DnaK proteins (56%). The aim of this work was to investigate the molecular basis of the observed interspecies chaperone interaction. Infrared resolution-enhanced spectra of the M. mazei and E. coli DnaK proteins were almost identical, indicating high similarity of their secondary structures, however, some small differences in band position and in the intensity of amide I' band components were observed and discussed. Profiles of thermal denaturation of both proteins were similar, although they indicated a higher thermostability of the M. mazei DnaK compared to the E. coli DnaK. Electrophoresis under non-denaturing conditions demonstrated that purified DnaK and GrpE of E. coli and M. mazei formed mixed complexes. Protein modeling revealed high similarity of the 3-dimensional structures of the archaeal and bacterial DnaK and GrpE proteins.
    Źródło:
    Acta Biochimica Polonica; 2007, 54, 2; 245-252
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Changes in expression of serine proteases HtrA1 and HtrA2 during estrogen-induced oxidative stress and nephrocarcinogenesis in male Syrian hamster
    Autorzy:
    Zurawa-Janicka, Dorota
    Kobiela, Jaroslaw
    Stefaniak, Tomasz
    Wozniak, Agnieszka
    Narkiewicz, Joanna
    Wozniak, Michał
    Limon, Janusz
    Lipinska, Barbara
    Powiązania:
    https://bibliotekanauki.pl/articles/1040768.pdf
    Data publikacji:
    2008
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    17-β-estradiol
    HtrA proteases
    estrogen-induced carcinogenesis
    oxidative stress
    Opis:
    Serine proteases HtrA1 and HtrA2 are involved in cellular stress response and development of several diseases, including cancer. Our aim was to examine the involvement of the HtrA proteins in acute oxidative stress response induced in hamster kidney by estrogen treatment, and in nephrocarcinogenesis caused by prolonged estrogenization of male Syrian hamster. We used semi-quantitative RT-PCR to estimate the HtrA1 and HtrA2 mRNA levels in kidney tissues, and Western blotting to monitor the amount of the HtrA proteins. Within the first five hours following estrogen administration both HtrA1 mRNA and the protein levels were increased significantly. No changes in the expression of HtrA2 were observed. This indicates that HtrA1 may be involved in the response against oxidative stress induced by estrogen treatment in hamster kidney. During prolonged estrogenization, a significant reduction of the HtrA1 mRNA and protein levels was observed after 6 months of estradiol treatment, while the expression of HtrA2 was significantly elevated starting from the third month. This suggests an involvement of the HtrA proteins in estrogen-induced nephrocarcinogenesis in hamster. Using fluorescence in situ hybridization we localized the HtrA1 gene at the qb3-4 region of Syrian hamster chromosome 2, the region known to undergo a nonrandom deletion upon prolonged estrogenization. It is possible that the reduced level of HtrA1 expression is due to this chromosomal aberration. A full-length cDNA sequence of the hamster HtrA1 gene was obtained. It codes for a 50 kDa protein which has 98 and 96% identity with mouse and human counterparts, respectively.
    Źródło:
    Acta Biochimica Polonica; 2008, 55, 1; 9-20
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Immune response against HtrA proteases in children with cutaneous mastocytosis
    Autorzy:
    Renke, Joanna
    Kędzierska-Mieszkowska, Sabina
    Lange, Magdalena
    Nedoszytko, Bogusław
    Liberek, Anna
    Plata-Nazar, Katarzyna
    Renke, Marcin
    Wenta, Tomasz
    Żurawa-Janicka, Dorota
    Skórko-Glonek, Joanna
    Lipińska, Barbara
    Powiązania:
    https://bibliotekanauki.pl/articles/1038382.pdf
    Data publikacji:
    2018
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    HtrA proteases
    children
    cutaneous mastocytosis
    mast cells
    Opis:
    Mast cells play an important role in both, the innate and adaptive immunity, however, clonal proliferation of abnormal mast cells in various organs leads to mastocytosis. A skin variant of the disease, cutaneous mastocytosis (CM) is the most frequent form of mastocytosis in children. HtrA proteases are modulators of important cellular processes, including cell signaling and apoptosis, and are related to development of several pathologies. The above and the observation that mast cells constitutively release the HtrA1 protein, prompted us to investigate a possible involvement of the HtrA proteins in pediatric CM. Levels of the serum autoantibodies (IgG) against the recombinant HtrA proteins (HtrA1-4) in children with CM (n=36) and in healthy controls (n=62) were assayed. Anti-HtrA IgGs were detected using enzyme linked immunosorbent assay (ELISA) and Western-blotting. In the CM sera, levels of the anti-HtrA1 and anti-HtrA3 autoantibodies were significantly increased when compared to the control group, while the HtrA protein levels were comparable. No significant differences in the anti-HtrA2 IgG level were found; for the anti-HtrA4 IgGs lower levels in CM group were revealed. In healthy children, the IgG levels against the HtrA1, -3 and -4 increased significantly with the age of children; no significant changes were observed for the anti-HtrA2 IgG. Our results suggest involvement of the HtrA1 and HtrA3 proteins in pediatric CM; involvement of the HtrA4 protein is possible but needs to be investigated further. In healthy children, the autoantibody levels against HtrA1, -3 and -4, but not against HtrA2, increase with age.
    Źródło:
    Acta Biochimica Polonica; 2018, 65, 3; 471-478
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Graphene oxide-based biomaterials as a potential tool for cartilage tissue regeneration
    Autorzy:
    Szkaradek, Agnieszka
    Sekuła-Stryjewska, Małgorzata
    Noga, Sylwia
    Pabiś, Marta
    Łabędź-Masłowska, Anna
    Karnas, Elżbieta
    Kmiotek-Wasylewska, Katarzyna
    Jagiełło, Joanna
    Lipińska, Ludwika
    Zuba-Surma, Ewa
    Powiązania:
    https://bibliotekanauki.pl/articles/284610.pdf
    Data publikacji:
    2019
    Wydawca:
    Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
    Źródło:
    Engineering of Biomaterials; 2019, 22, no.153; 45
    1429-7248
    Pojawia się w:
    Engineering of Biomaterials
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
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