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Wyświetlanie 1-2 z 2
Tytuł:
Influence of protein deficient diet, vitamin B2 supplementation and physical training on serum composition of polyunsaturated fatty acids (PUFAs) in rats
Autorzy:
Lewicka, Aneta
Lewicki, Sławomir
Kłos, Anna
Dębski, Bogdan
Kurył, Tomasz
Bertrandt, Jerzy
Powiązania:
https://bibliotekanauki.pl/articles/989797.pdf
Data publikacji:
2017
Wydawca:
Instytut Medycyny Wsi
Tematy:
protein malnutrition
vitamin b2
training
pufa
Opis:
Introduction. Prolonged shortages of protein in the diet significantly alter the composition and content of polyunsaturated fatty acids (PUFA) in tissues and body fluids. One of nutritional factors which may reduce negative effects of protein malnutrition might be vitamin B2 due to its influence on lipids metabolism. Objective. The aim of the study was to investigate the influence of low protein (LP) diet enriched with vitamin B2 on the content and composition of PUFA in the blood serum of rats treated with dosed physical exercise. Materials and method. The experiment was carried out for 3 months on 72 growing male Wistar rats divided into 5 groups. Animals were fed ad libitum on a diet with an energy value of 350 kcal/100 g, in which 4.5% of the energy was provided by protein. In the control diet, 20% of the energy was provided by protein. Two groups were fed the diet enriched with vitamin B2. The two groups of tested animals were trained for 5 days a week. Results. LP diet caused a decrease in α-linolenic acid (ALA) after 30 days, and a decrease in docosahexaenoic acid (DHA) after 60 days of experiment, compared with rats fed the control diet. After 60 and 90 days of the experiment, a significant decrease was noted in arachidonic acid (AA) in serum of trained rats, compared with sedentary rats fed the LP diet. Physical activity increased LA (mainly on day 30), EPA (on day 90) and reduced AA content (on day 90) in serum of rats fed the LP diet. B2 supplementation in the trained LP group did not change the EPA and AA dependence; however, there was a decrease in LA content in comparison to the non-supplemented trained group. Conclusions. Results of this study suggest that all investigated factors (protein deficiency, physical exercise and supplementation of vitamin B2) have significant impact on PUFA composition of serum in rats.
Źródło:
Annals of Agricultural and Environmental Medicine; 2017, 24, 2
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Extremely low-frequency electromagnetic field (ELF-EMF) induced alterations in gene expression and cytokine secretion in clear cell renal carcinoma cells
Autorzy:
Cios, Aleksandra
Ciepielak, Martyna
Lieto, Krystyna
Matak, Damian
Lewicki, Sławomir
Palusińska, Małgorzata
Stankiewicz, Wanda
Szymański, Łukasz
Powiązania:
https://bibliotekanauki.pl/articles/31340344.pdf
Data publikacji:
2024-05-21
Wydawca:
Instytut Medycyny Pracy im. prof. dra Jerzego Nofera w Łodzi
Tematy:
electromagnetic field
renal carcinoma
ADAM28
VEGFC
NCAM1
TGF-β1
Opis:
Background The study aimed to investigate the influence of extremely low-frequency electromagnetic fields (ELF-EMF) on clear cell renal cell carcinoma (ccRCC) by assessing alterations in gene expression and the secretion of cytokines and chemokines. Material and Methods Three ccRCC cell lines (786-O, 769-P, and CAKI-1) and a healthy HEK293 cell line were subjected to ELF-EMF exposure (frequency 50 Hz, magnetic field strength 4.5 mT) for 30 min daily for 5 days. The study examined the expression of ADAM28, NCAM1, and VEGFC genes, along with the secretion of 30 cytokines and chemokines. Results Notably, primary tumor-derived cell lines, but not those from metastatic sites, exhibited ADAM28 gene expression, which increased following ELF-EMF exposure. A statistically significant reduction in VEGFC gene expression was observed in 769-P cells after ELF-EMF exposure. Additionally, NCAM1 gene expression was upregulated in HEK293, 769-P, and 786-O cells, representing normal embryonic kidney cells and primary tumor cells, but not in CAKI-1 cells, which model metastatic sites. After EMF exposure, there was a statistically significant decrease in transforming growth factor β1 (TGF-β1) concentration in the cell culture supernatants of HEK293 and CAKI-1 cell lines, with no other significant changes in the secretion of tested cytokines. Conclusions Given the study’s findings and available research, caution is warranted when drawing conclusions about the potential inhibitory effect of ELF-EMF on ccRCC progression. Standardization of experimental models is imperative when assessing the effects of EMF in a human context.
Źródło:
Medycyna Pracy. Workers’ Health and Safety; 2024, 75, 2; 133-141
0465-5893
2353-1339
Pojawia się w:
Medycyna Pracy. Workers’ Health and Safety
Dostawca treści:
Biblioteka Nauki
Artykuł
    Wyświetlanie 1-2 z 2

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