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    Wyświetlanie 1-2 z 2
    Tytuł:
    Column chromatography as a useful step in purification of diatom pigments
    Autorzy:
    Tokarek, Wiktor
    Listwan, Stanisław
    Pagacz, Joanna
    Leśniak, Piotr
    Latowski, Dariusz
    Powiązania:
    https://bibliotekanauki.pl/articles/1038760.pdf
    Data publikacji:
    2016
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    column chromatography
    Phaeodactylum tricornutum
    fucoxanthin
    diadinoxanthin
    diatoxanthin
    thin layer chromatography
    Opis:
    Fucoxanthin, diadinoxanthin and diatoxanthin are carotenoids found in brown algae and most other heterokonts. These pigments are involved in photosynthetic and photoprotective reactions, and they have many potential health benefits. They can be extracted from diatom Phaeodactylum tricornutum by sonication, extraction with chloroform : methanol and preparative thin layer chromatography. We assessed the utility of an additional column chromatography step in purification of these pigments. This novel addition to the isolation protocol increased the purity of fucoxanthin and allowed for concentration of diadinoxanthin and diatoxanthin before HPLC separation. The enhanced protocol is useful for obtaining high purity pigments for biochemical studies.
    Źródło:
    Acta Biochimica Polonica; 2016, 63, 3; 443-447
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Results of sentinel lymph node biopsy in patients with breast cancer in 10-year own material of the 4th Military Teaching Hospital with Polyclinic in Wrocław
    Autorzy:
    Kabziński, Piotr
    Rac, Jacek
    Dorobisz, Tadeusz
    Pawłowski, Wiktor
    Ziomek, Agnieszka
    Chabowski, Mariusz
    Janczak, Dawid
    Leśniak, Michał
    Janczak, Dariusz
    Powiązania:
    https://bibliotekanauki.pl/articles/1394134.pdf
    Data publikacji:
    2016
    Wydawca:
    Index Copernicus International
    Tematy:
    breast cancer
    sentinel lymph node
    radioisotope
    lymphoscintigraphy
    Opis:
    At present, sentinel lymph node biopsy is a standard procedure to assess the advancement of breast cancer and cutaneous melanoma. The aim of the study was to assess the role of the sentinel lymph node biopsy in the treatment of patients with breast cancer in our own material. Material and methods. Analyzed was medical documentation of 258 patients with initially operable breast cancer, qualified for operation with sentinel lymph node biopsy in 2004-2014 in the Department of Surgery of the 4th Military Teaching Hospital. A few hours prior to the planned surgery, radioisotope (technitium-99 sulfur colloid) was applied in the area of tumor or under the areola. 1-2 hours after administering the tracer, the lymphoscintigraphy with the labelling of the sentinel lymph node on the skin was performed. Results. On the basis of the gathered material, obtained were the following parameters: sensitivity – 100%, and specificity – 94.6%. Four cases were false negative (5.5%). Conclusions. 1. Marking the sentinel lymph node in breast cancer, based on the single visualisation method with the use of radioisotope, is a useful and effective technique. 2. The factor influencing the results of the sentinel lymph node biopsy (true positive and negative results and false negative result) was the number of the excised lymph nodes except for the sentinel lymph node. 3. Patients with estrogen receptor expression had often metastases to sentinel lymph node (145 cases – 56%). 4. The false negative rate, i.e. 5.5% in our material, is within the limits of acceptability given in the literature. 5. The sentinel lymph node biopsy performed by the experienced surgical team is a reliable diagnostic tool with a low complication rate.
    Źródło:
    Polish Journal of Surgery; 2016, 88, 3; 130-135
    0032-373X
    2299-2847
    Pojawia się w:
    Polish Journal of Surgery
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-2 z 2

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